The prevalence of antibodies against desmoglein 1 in endemic pemphigus foliaceous in Brazil

Background - Pemphigus foliaceus is an autoimmune skin disease mediated by autoantibodies against desmoglein 1. The endemic form is thought to ahve an environmental cause. The Terena reservation of Limao Verde in Mato Grosso do Sul, Brazil, is a recently identifies focus of the disease, with a prevalence of 3.4 percent in the population. We tested the hypothesis taht normal subjects living in an endemic area have antibodies agaisnt desmoglein 1. Methods - We used an enzyme-linked immunosorbent assay to defect antibodies agaisnt desmoglein 1 in serum samples from 60 patients with endemic pemphigus foliaceus (fogo selvagem) who lived in Limao Verde or elsewhere in Brazil, 372 normal subjects (without pemphigus foliaceus) from Limao Verde and surrounding location, and 126 normal subjects from the United States and Japan. Results - Antibodies against desmoglein 1 were detected in 59 of the 60 patients with fogo selvagem (98 percent) but in only 3 of the 126 normal subjects from the United States and Japan (2 percent). Antibodies were also detected in 51 of the 93 normal subjects from Limao Verde (55 percent) and in 54 of the 279 normal subjects from surrounding areas (19 percent). Serum samples obtained one to four years before the onset of disease were available for five patients; all five had antibodies in the initial serum serumsamples, and the onset of disease was associated with a marked increase in antibody values. Conclusions - The prevalence of antibodies against desmoglein 1 is high among normal subjects living in an area where fogo selvagem is endemic, and the onset of the disease is preceded by a sustained antibody response. These findings support the concept that the production of antibodies against desmoglein 1 is initiated by exporure to an unknown environmental agent

Comparison of black fly species (Diptera: simuliidae) on an amerindian reservation with a high prevalence of Fogo Selvagem to neighboring disease-free sites in the State of Mato Grosso do Sul, Brazil

Fogo selvagem is an autoimmune blistering skin disease that principally occurs among rural Brazilians living in geographically clumped disease foci. Exposure to hematophagous black flies possibly is related to the cause of the disease. We compared the occurrence, proportions, and richnees of simuliid species immatures and the biting activity of adult females within a recently discovered, high prevalence focus of fogo selvagem, the Limao Verde Terena Reservation, to that of neighboring regions with no reported cases of fogo selvagem. Nine black fly species were collected from 12 stream sites during 5 trips to the fogo selvagem focus. The species showed longitudinal (upstream-downstream) trends in occurrence, proportions, and richness, and the abundant simuliid immatures was greater at downstream sites. The most prevalent species at the focus, Simulium nigrimanum (Macquart), dominated the stream sites with highly abundant simuliid aseemblages, and was the most common black fly in human bait collections. This species was absent or in very low numbers in neighboring valleys and villages that did not have cases of fogo selvagem

Anti-desmoglein-1 antibodies in onchocersiasis, leishmaniosis and chagas disease suggest a posible etiological link to fogo selvagem

It presents a study that search for anti-desmoglein-1 antibodies in sera from parasitic (leishmaniosis, chagas diseases and onchocerciasis), and infectious disease wicth are prevalent in the same goegraphic regions of Brazil. Document in PDF format, required Acrobat Reader.