RESUMEN
Substances for manufacture of parenteral drugs require control by the "Bacterial Endotoxin" (BE) index with the LAL-test. The aim of the study was to show possible applicability of organic solvents in BE determination by the gel-thromb test in case of water-insoluble pharmaceutical substances. The results confirmed that ethyl alcohol practically had no effect on the endotoxin activity. In the routine assays it is advisable to test solutions of the substances at the alcohol concentration of 6% below.
Asunto(s)
Endotoxinas/análisis , Prueba de Limulus , Preparaciones Farmacéuticas/análisis , Control de Calidad , Sensibilidad y Especificidad , SolubilidadAsunto(s)
Inyecciones Intraperitoneales/métodos , Inyecciones Intravenosas/métodos , Preparaciones Farmacéuticas/administración & dosificación , Pruebas de Toxicidad Aguda/normas , Animales , Antibacterianos/administración & dosificación , Cefepima , Cefalosporinas/administración & dosificación , Relación Dosis-Respuesta a Droga , Inyecciones Intraperitoneales/normas , Inyecciones Intravenosas/normas , Ratones , Factores de TiempoAsunto(s)
Antibacterianos/toxicidad , Animales , Antibacterianos/administración & dosificación , Antibacterianos/normas , Cefazolina/administración & dosificación , Cefazolina/normas , Cefazolina/toxicidad , Cefoperazona/administración & dosificación , Cefoperazona/normas , Cefoperazona/toxicidad , Ceftriaxona/administración & dosificación , Ceftriaxona/normas , Ceftriaxona/toxicidad , Contaminación de Medicamentos , Dosificación Letal Mediana , Ratones , Farmacopeas como Asunto , Pruebas de Toxicidad AgudaAsunto(s)
Ácido Fólico/análisis , Análisis de los Alimentos , Acetatos , Bioensayo , Queso/análisis , Precipitación Química , Enterococcus faecalis , Fermentación , Fluorometría , Métodos , Permanganato de Potasio , Cloruro de Sodio , Glycine max/análisis , Ácido Tricloroacético , Tirosina , Verduras/análisisRESUMEN
Dynamics of distribution of anabolic steroidal hormone methandrostenolone and routes of its elimination from the organism of Wistar rats were studied by using methods of radioisotopes and high-performance liquid chromatography. Methandrostenolone metabolites were shown to be excreted mainly in the urine. Methandrostenolone metabolism is a complicated process in the course of which redistribution of metabolites among various organs occurs. The anabolic effect of methandrostenolone is supposed to be due to the formation of its metabolites.
Asunto(s)
Metandrostenolona/farmacocinética , Animales , Biotransformación , Cromatografía Líquida de Alta Presión , Heces/análisis , Masculino , Metandrostenolona/análisis , Ratas , Ratas Endogámicas , Factores de Tiempo , Distribución Tisular , TritioRESUMEN
For studying distribution of drugs and their metabolites in organs of experimental animals by the method of radioactive indicators with recording of measurements of the compounds under study by means of a liquid-scintillaton counter, there is described a simple and available method of complete solubilization of a biological tissue. The method makes it possible to dissolve up to 10 g of a biological tissue without preliminary lyophilization.
Asunto(s)
Farmacocinética , Conteo por Cintilación/métodos , Animales , Solubilidad , Distribución TisularRESUMEN
Metabolism of the anabolic steroidal hormone methandrostenolone in Wistar rats was studied by high performance liquid chromatography (HPLC) and redioisotope techniques. The conditions for isolation of methandrostenolone metabolites by HPLC were developed. Reduction of the double bonds was shown to be an early stage in methandrostenolone biotransformation. The main portion of the metabolites was excreted with urine as glucuronide conjugates. No sulfoesters were found.