Exploring the vulnerability of the coastal wetlands of India to the changing climate and their adaptation strategies.

Changing climate exerts significant pressure on coastal wetland fishery, ecosystems and livelihood at the eco-regional level. In the present study, an indicator and scoring-based vulnerability assessment framework was developed to assess climate change-induced potential impacts and vulnerability of the coastal wetlands through stakeholder perception and primary data collection on ecology and fisheries. The study was conducted in 12 coastal wetlands in the Gangetic estuarine regions of eastern India. Analysis of rainfall, temperature and extreme climatic events was also carried out. The climate variability in the region was analysed and discussed in the context of ecosystem health and sustainability indicators. The results indicated that anthropogenic stress has a greater influence on ecosystem services than climatic stress. However, the nature and degree of vulnerability of fishers to climate change vary with their capacity to adapt to such changes and alternate sources of livelihood. Altogether, 34 fish species were documented, belonging to 11 taxonomic orders, and categorized as per intensity of resilience using selected criteria. Perception-based climate-resilient adaptation strategies have also been discussed. Developing appropriate adaptation policies and implementing climate-resilient practices and management can minimize vulnerability and increase the adaptive capacity of fishers ensuring sustainable livelihood options.

A new snake eel species of the genus Ophichthus (Anguilliformes: Ophichthidae) from the southeast coast of India, Bay of Bengal, with the taxonomic account of Ophichthus chilkensis.

A new species of the ophichthid eel of the family Ophichthidae is described based on five specimens collected from the Mudasalodai fish landing center, off Cuddalore coast, southeast coast of India, Bay of Bengal. Ophichthus naevius sp. nov. is distinguished from its congeners by having a unique color pattern: dorsal body with numerous dense dark spots or patches, ventral body pale yellowish green, dorsal-fin origin just before pectoral-fin tip, vertebral formula: 12-14/52-53/134-138, and teeth on jaw uniserial and pointed. The study also reports the range extension and molecular evidence of Ophichthus chilkensis from South India. Molecular analyses were performed for both species, and their phylogenetic relationship suggests that the new species exhibits 10.2% genetic divergence with its congener Ophichthus sangjuensis, followed by Ophichthus brevicaudatus (10.4%), and Ophichthus sp. 1 (11.8%) also forms the closest clade in both Bayesian inference and maximum likelihood (ML) tree. Similarly, according to the topology of the ML tree, the species O. chilkensis forms a clade with Ophichthus sp. 5, Ophichthus remiger, Ophichthus frontalis, Ophichthus sp. 6, and Ophichthus rex, suggesting that it would be the genetically closest congener.

The concept of "state fishes of India": Current status, knowledge gaps, and strategic plans for conservation and sustainable utilization.

The concept of "state fishes of India" highlights the importance and significance of the prioritized fish species distributed across various states within the country. This review article systematically documents the current status of state fishes from various perspectives, identifies the prevailing knowledge gaps, and also highlights the issues and strategic plans essential for the conservation and sustainable utilization of these valuable genetic resources. A total of 8357 publications were checked for the consolidated information on state fish species, and the appropriate items were selected under eight categories: biology, physiology and nutrition, aquaculture, habitat and environmental parameters, genetics and biotechnology, harvest and postharvest, fish health management, and others. The synthesized information was used to present the current status of research and development on state fish species. The knowledge gaps that are to be addressed are also depicted under the perspectives of fisheries management and conservation aquaculture. Based on the findings, strategic plans for the targeted conservation programmes are proposed and discussed under various in situ and ex situ conservation measures. Further, the departmental processes involved in the declaration, the importance of stakeholder involvement, namely, local communities and policymakers, in fostering effective conservation measures, and planning for utilization of these valuable fish genetic resources are also indicated.

Schistura sonarengaensis, a new species of cave-dwelling loach (Teleostei: Nemacheilidae) from Meghalaya, northeast India.

A new species of nemacheilid loach, Schistura sonarengaensis sp. nov., is described from three cave-dwelling populations (Barak-Surma-Meghna drainage) in the South Garo Hills district of Meghalaya, India. The new species possesses prominent eyes but is easily distinguished from all the congeners of the genus Schistura from Barak-Surma-Meghna and adjacent rivers drainages of northeast Indian (except S. syngkai) in having 13-26 vertically elongated to circular mid-lateral black blotches (brownish in life) overlayered on a grayish-black mid-lateral stripe on a dull white or pale-beige (golden brown in life) body. However, it can be easily distinguished from S. syngkai in having a complete (vs. incomplete) lateral line with more 72-89 (vs. 19-42) lateral-line pored scales, greater pre-dorsal length (48.5-53.1 vs. 41.9-44.1 %SL), a wider body at dorsal-fin origin (11.3-16.7 vs. 9.4-10.3 %SL), greater dorsal (18.1-21.1 vs. 15.1-17.0 %SL) and lateral (20.9-24.1 vs. 17.4-18.9 %SL) head length, a wider head (14.5-18.5 vs. 11.6-13.3 %SL), and moderately forked (vs. emarginated) caudal fin. Further, molecular analysis confirms the distinctiveness of S. sonarengaensis sp. nov. from its congeners found in northeast India by significant divergences with uncorrected genetic distance ranging from 3.7% to 17.3% in the mitochondrial cytochrome c oxidase subunit I (COI) gene dataset. The phylogenetic position of the new species with its sister species was evaluated using maximum likelihood and Bayesian analysis. The species delimitation approaches assemble species by automatic partitioning (ASAP) and Poisson tree processes (PTP) utilized for testing species assignments consistently identified our test group as a distinct species from its sister species. Although the new species lacks typical morphological adaptations usually associated with a subterranean life, such as complete absence (or vestigial presence) of eyes and pigmentation, it exhibits a reduction of pigmentation when compared to the epigean congeners.

Insulin-like growth factor II, a marker gene for determining the optimum dietary protein level in clownfish Amphiprion ocellaris.

In vivo study was conducted to determine the role of insulin-like growth factors (IGF-II) related to dietary protein utilization. For this early juvenile stage of marine false clownfish Amphiprion ocellaris, 300 numbers of 20-day-old larvae (initial body weight of 18.2±0.027 mg/fish) were used as an experimental animal. Animals were fed for 12 weeks with different dietary protein levels (35, 40, 45, 50, and 55%), using Spirulina maxima as a major protein source. Proximate compositions and amino acid profile of the formulated diet were analyzed by the standard methods. Eventually, significant (P<0.05) mean, body weight gain, absolute growth rate, specific growth rate, and feed conversion ratio were obtained in the fishes fed with 50% of dietary protein, whereas, poor growth performance was noticed in 35% of the dietary protein level fed juveniles. The growth-responsible gene IGF-II expression studies showed the significant upregulation in the growth of the juveniles at 2.05±0.11 for 40%, 3.13±0.20 for 45%, 4.97±0.13 for 50%, and 4.33± 0.24 for 55%, which were higher than the control group 35%. The study concluded that 50% of dietary protein level is optimal for better growth indices in clownfish; A. ocellaris juveniles and IGF-II can be used as a potential marker gene to assess the growth indices in A. ocellaris.

Exploring vulnerabilities of inland fisheries in Indian context with special reference to climate change and their mitigation and adaptation: a review.

Tropical inland capture fisheries are susceptible to a series of vulnerabilities such as habitat destruction, biodiversity loss, pollution, overfishing, invasive species and anthropogenic climate change. A comprehensive review of the impact of climatic uncertainties on Indian inland fisheries has not been adequately attempted yet. Recent approaches emphasizing ecosystem-based management in a regional context, specific to inland fisheries for combating climatic changes, have not been reported to date. The paper presents a critical bibliometric review of the climatic vulnerabilities faced by Indian inland fishery resources and various adaptive and mitigation strategies put forward by the country for the sustainability of the resources. In this communication, a systematic review of the impact of climate change and other stressors on various inland ecosystems of the subcontinent and the ecosystem-based management strategies adopted in India is presented and discussed.

A new species of the conger eel genus Macrocephenchelys from deep waters of the Arabian Sea.

A new species of the genus Macrocephenchelys is described herein based on a single specimen collected from the deep-sea trawl landing at Kalamukku fish landing centre, Kerala coast, Arabian Sea. The new species is distinguished by having a dorsal-fin origin behind the middle of pectoral fin, a larger head, shorter trunk, larger gill opening, dorsal surface of body with dark-brown colour and ventral surface of head and belly with numerous patches of melanophores before anus, vertebrae 14-30-151. The new species shares most of the characteristics with Macrocephenchelys brevirostris but differs from it by having a more anterior dorsal-fin origin (vs. over the tip or slightly behind the pectoral-fin tip), larger head [15.3% total length (TL) vs. 10.5%-13.9% TL, 53.2% pre-anal length (PAL) vs. 35.8%-47.6% PAL], shorter trunk length (13.6% TL vs. 14.4%-20.6% TL, 47.3% PAL vs. 52.4%-66.2% PAL); further it shows 7.9%-8.1% genetic divergence from the sequences of M. brevirostris.

Altitudinal and seasonal distribution of benthic macroinvertebrates in River Tons - a tributary of Yamuna River, Uttarakhand, India.

The main tributary of the Yamuna, the Tons River, exhibits altitudinal changes in its macroinvertebrate community's diversity, abundance, and composition. Between May 2019 and April 2021, the study was conducted in the upper section of the river. A total of 48 numbers of taxa from 34 families and ten orders were recorded during the investigation. At this elevation of 1150 to 1287 m, the two most predominant orders are Ephemeroptera (32.9%) and Trichoptera (29.5%). During the premonsoon season, they had the lowest macroinvertebrate density (250-290 individuals/m2), and the post-monsoon season had the highest density (600-640 individuals/m2). During the post-monsoon season, the maximum larval forms (60%) of various insect orders were predominant. The findings indicated that lower altitudes (1150-1232 m) have higher macroinvertebrate abundance than higher ones. The diversity of dominance is shallow at site-I (0.0738) and strong at the site-IV during the premonsoon season (0.03837). Taxa richness, as measured by the Margalef index (D), peaked in the spring season (January to March) at 6.9 and reached its lowest point (5.74) in the premonsoon season (April to May). Only 16 taxa were discovered in site-I and site-II, but 39 taxa were discovered at low altitudes (site-IV, 1100 m) (1277-1287 m). The Tons River contains a total of 12 and 13 genera, respectively, that belong to the orders Ephemeroptera and Trichoptera, according to qualitative study of the macroinvertebrates. The current study supports the use of macroinvertebrates as bioindicator species for monitoring biodiversity and assessing the health of ecosystems.

Combining stakeholder perception and ecological approaches for assessing vulnerability of floodplain wetlands in changing climate: a regional study.

Wetland fisheries are most vulnerable to climate and anthropogenic change, and therefore, vulnerability assessment is essential for the formulation of prudent management strategies. In the present study, vulnerability assessment was carried out in nine floodplain wetlands from three districts of West Bengal using stakeholder perception and ecological conditions. In absence of long-term time series data on ecology and fisheries of wetlands, stakeholder perception study was carried out to assess the vulnerability status to climate change. Phased interviews were conducted using structured questionnaires to study the stakeholder perception on climatic variability and changes in ecology and fisheries of wetlands over the past 15 years. Climate data used for climate trend analysis was procured from Indian Meteorological Department. Ecological parameters were studied from the wetlands seasonally from January 2018 to December 2018. Analysis of climatic variables for last three decades revealed a warming trend and decreasing rainfall in the study area. The temperature anomaly ranged from + 0.07 to + 0.31 °C while rainfall anomaly ranged from - 61.41 to - 372.62 mm. Respondents showed high level of consensus (75.3%) on climate change awareness. The respondents were aware of the fact that climate anomaly affects ecology and fisheries of wetlands in most of the cases with consensus ranging from 67.8 to 94.7%. The studied wetlands showed 2 to 81.28% reduction in depth, 21.52-61.29% reduction in species diversity, and 30-95% macrophyte infestation. The stakeholder perception-based vulnerability scores ranged from 18 to 31 and indicated 6 out of 9 wetlands (66.6%) as highly vulnerable and the rest as moderately vulnerable. The ecological vulnerability scores ranged from 17 to 21 and indicated 5 out of 9 wetlands (55.6%) as highly vulnerable and 4 wetlands in the moderately vulnerable range. Although both the approaches used in the present study categorized the studied wetlands as moderately to highly vulnerable, the category of some of the wetlands varied in both the approaches. This suggests that utilizing a single approach may not give precise vulnerability status of an ecosystem. Use of combined approaches for construction of a composite vulnerability index covering different aspects impacted by climate change might present a better picture of the vulnerability status and aid in formulation of effective mitigation/management plan.

Inland heat waves (IHWs) and associated impacts on hydro-biology of aquatic ecosystems in lower Ganga basin, India.

The present study assessed the occurrence and impact of heat waves on the ecology of two ecosystems namely Bhomra wetland and Ganga River stretch, India. The water samples collected from these ecosystems were analyzed for estimating the hydrological and biological variables during heat wave. The inland heat index (IHI) was derived from the climatic variables, relative humidity and temperature. The study indicated the predominant and periodic occurrence of inland heat waves (IHW) with indices ranging from 34.8 to 42.8 °C and 35.9 to 43.5 °C at the Bhomra and Ganga River stretch respectively during the summer months (March-June). The first two components of the principal component analysis of physico-chemical parameters and heat index explained 45.6% and 59% of the variation in the Bhomra and Ganga River stretch respectively. PCA showed a similar pattern in variation of IHWs and dissolved oxygen, nutrients, hardness and alkalinity, but a distinct pattern with conductivity and TDS in the wetland. IHW exhibited a similar pattern of variation with TDS, conductivity, dissolved oxygen, pH and hardness and distinct pattern with alkalinity, phosphate and nitrate in the river stretch. The first two components of PCA of IHI and plankton abundance explained 89% of the variation and IHI had a similar pattern of variation with the abundance of diatoms and a diverse pattern of variation with blue-green and green algae in the studied ecosystems which might affect the food availability of the associated fishes. The study suggests that IHW influences the water quality and primary producers and also summarizes the impact of IHW on ecosystem services and necessitates mitigation measures.

Blood Pressure Variation with Altitudes in Children: A Cross-Sectional Observational Study from Himalayan Hills.

OBJECTIVES: We aimed to evaluate blood pressure (BP) in Indian children who had similar demographic characteristics but hailed from different altitudes. METHODS: BP of school going children, aged 5 to 12 years, at five different locations varying in altitude (near sea level: n = 425; 2000 feet: n = 244; 4000 feet: n = 248; 6000 feet: n = 242 and 8000 feet: n = 250) was measured in a mountainous district in Himalaya. Systolic blood pressure (SBP) and diastolic blood pressure (DBP) were recorded by aneroid sphygmomanometer in the sitting posture using a calibrated instrument and four trained resident doctors. The average of three readings was taken. RESULTS: Of the 1229 children whose data were analyzed, 50.4% were boys. SBP showed a progressive rise from 99.5 (4.00) mmHg [mean (standard deviation)] at near sea level to 106.7 (4.17) mmHg at 8000 feet. Similarly, DBP showed a rise from 60.1 (3.67) mmHg to 66.8 (4.58) mmHg over the same altitude range. Analysis of covariance for BP variation with altitude, with age as covariate, indicated a modest but statistically significant rise in both SBP and DBP with altitude. Higher BP values was noted among children residing at higher than at lower altitude (0.8% at sea level to 18.8% at 8000 feet). Correlations between SBP and DBP values and height and weight, though positive and significant at p < 0.01 level, were weak. CONCLUSIONS: These data will help in correct interpretation of BP readings in children residing at high altitude.

BAC-FISH Based Physical Map of Endangered Catfish Clarias magur for Chromosome Cataloguing and Gene Isolation through Positional Cloning.

Construction of a physical chromosome map of a species is important for positional cloning, targeted marker development, fine mapping of genes, selection of candidate genes for molecular breeding, as well as understanding the genome organization. The genomic libraries in the form of bacterial artificial chromosome (BAC) clones are also a very useful resource for physical mapping and identification and isolation of full-length genes and the related cis acting elements. Some BAC-FISH based studies reported in the past were gene based physical chromosome maps of Clarias magur (magur) to understand the genome organization of the species and to establish the relationships with other species in respect to genes' organization and evolution in the past. In the present study, we generated end sequences of the BAC clones and analyzed those end sequences within the scaffolds of the draft genome of magur to identify and map the genes bioinformatically for each clone. A total of 36 clones mostly possessing genes were identified and used in probe construction and their subsequent hybridization on the metaphase chromosomes of magur. This study successfully mapped all 36 specific clones on 16 chromosome pairs, out of 25 pairs of magur chromosomes. These clones are now recognized as chromosome-specific makers, which are an aid in individual chromosome identification and fine assembly of the genome sequence, and will ultimately help in developing anchored genes' map on the chromosomes of C. magur for understanding their organization, inheritance of important fishery traits and evolution of magur with respect to channel catfish, zebrafish and other species.

Comparative assessment of carbon sequestration potential of different types of wetlands in lower Gangetic basin of West Bengal, India.

Wetlands provide a great ecological service by accumulating and sequestering carbon in their soils and thus help in mitigating climate change caused due to global warming. However, the capacity and efficiency of different types of wetlands vary considerably depending upon the nature of the wetland, hydrology, biogeochemistry, climatic condition, and many other factors. In the present paper, we have studied the carbon accumulation and sequestration in three different wetlands, one sewage fed, and two floodplain oxbow lakes in the West Bengal state of India. The selected wetlands vary in terms of ecological regimes such as water volume, depth, link channel, agricultural runoffs, primary productivity, macrophyte coverage, and fishery. The carbon accumulation in the wetlands, which varied from 48.53 to 143.17 Mg/ha up to 30-cm depth of soil, was much higher than that in the corresponding upland sites. The difference was much higher in the floodplain wetlands. So the study revealed that wetlands are better carbon sinks than the corresponding reference sites and the carbon sequestration potential varies according to the type of wetlands. A positive correlation was also observed between macrophyte coverage and the amount of C stored in the wetlands.

Dynamics of phytoplankton community in seasonally open and closed wetlands in the Teesta-Torsa basin, India, and management implications for sustainable utilization.

The present study deals with the broader understanding of phytoplankton assemblage pattern and their ecohydrological interactions in two ecologically distinct floodplain wetlands of Teesta - Torsa basin, India. Analyses of data revealed significant seasonal variations (p ≤ 0.05) of ten water variables (temperature, transparency, pH, conductivity, total dissolved solids, dissolved oxygen, total hardness, total alkalinity, PO4 - P, and SiO4 - Si) in both the wetlands; however, no significant variation was observed among the sampling stations. In total, 128 species of phytoplankton were recorded (118 species belonging to 94 genera in seasonally open; 103 species belonging to 86 genera in closed wetland). Four algal groups, viz. Cyanophyceae, Coscinodiscophyceae, Bacillariophyceae, and Chlorophyceae, were the dominant quantitative component, remarkably influencing the total phytoplankton population in both the wetlands, contributing ~ 87% of total phytoplankton. Species Aulacoseira granulata alone contributed 12 - 41% and 8 - 34% to the total phytoplankton in the seasonally open and closed wetland, respectively, and indicated high organic load in both the wetlands. Altogether thirty-six and thirty-one phytoplankton taxa appeared as major indicators across the seasons for seasonally open and closed wetland, respectively. The indicator taxa (Aulacoseira, Oscillatoria, Dolichospermum, Spirogyra, Synedra, Nitzschia, Navicula, Euglena, Phacus) in both the wetlands hinted that the wetlands are under pollution pressure. The assemblage structure of phytoplankton was related to transparency, NO3 - N, PO4 - P, SiO4 - Si, total dissolved solids, and temperature as evident from BIO - ENV. Furthermore, the marginal test also selected similar variables (depth, transparency, conductivity, PO4 - P, SiO4 - Si) for seasonally open and the variables such as depth, conductivity, total dissolved solids, total alkalinity, and NO3 - N for the closed wetland. The study showed that the seasonal riverine connectivity greatly influences the variations in phytoplankton community in the seasonally open wetland.

Modeling pre-spawning fitness and optimal climate of spotted snakehead Channa punctata (Bloch, 1793) from a Gangetic floodplain wetland of West Bengal, India.

The spawning and well-being of fish in an ecosystem are closely linked to climatic cues, viz., temperature and rainfall. Reduced fitness can affect the reproductive performance and lead to skipped spawning. Benchmarking the threshold fitness required for a fish population to achieve readiness for spawning, and understanding how climatic parameters influence the fitness will aid in predicting the fate of its reproductive success in future climatic conditions. This study determined the threshold condition factor pre-spawning fitness (Kspawn50) at which 50% of the female Channa punctata population can be deemed fit for spawning. The optimal climate within which pre-spawning fitness is attained by this species under Indian climatic conditions was also identified. The study was conducted from June 2015 to September 2016, covering two spawning seasons (June-August) in a Gangetic floodplain wetland of West Bengal, India. The non-parametric Kaplan-Meier method (survival fit) was used for estimation of pre-spawning fitness. "Ready to spawn" females were classified based on binary coding of the gonadal maturity stages. The thermal and precipitation range within which spawning fitness is achieved was identified by using the locally weighted smoothing technique. Female C. punctata pre-spawning fitness (Kspawn50) ranged from 1.26 to 1.39 with an estimated median of 1.29 units. Temperatures between 29 and 32 °C and rainfall above 100 mm were conducive to attaining the requisite pre-spawning fitness in C. punctata. This is the first study benchmarking the pre-spawning fitness and optimal climate for C. punctata. Understanding spawning requirements can inform the climate change-induced impacts on reproductive plasticity and evolutionary adaptations of snakeheads in the Ganga river basin.

Pattern of the state of eutrophication in the floodplain wetlands of eastern India in context of climate change: a comparative evaluation of 27 wetlands.

The floodplain wetlands in different regional settings vary with time and space in terms of function and geomorphological diversity. In recent decades, these eco-sensitive waterbodies have been exposed to a wide range of anthropogenic threats and climatic changes. Therefore, assessment of these ecological and environmental threats is prerequisite to understand the state of ecosystem and to develop a sustainable management strategy for conservation of wetland biodiversity and fisheries enhancement. This paper discusses the region-specific pattern of trophic state index (TSI) of the 27 floodplain wetlands in West Bengal, India. Carlson TSI and Lamparelli TSI methods were used to determine a better approach based on historical and continuous dataset and to delineate the interrelationship among historical climatic variability for sustainable management of the resources. The study revealed that agro-climatic divisions do not unveil any significant impact on the TSI calculated using Carlson TSI as well as Lamparelli TSI method. The TSI scores for the two methods were significantly different (p < 0.01) for different zones based on wetland habitat types. The TSI scores revealed most of the wetlands to be in mesotrophic state. Principal component analysis (PCA) indicated that TSI scores were having similar pattern of variation with rainfall and water temperature. The present study also conveys fundamental information on ecological status based on the trophic state, which will aid to develop region-specific strategies for sustainable fisheries enhancement.

Surface Enhanced Raman Scattering Study of 1-H-2 (tolylazo) Imidazole (TaiH) Induced by Uncoupled Plasmon of Silver Nano Particles.

Raman and SER spectra of 1-H-2 (tolylazo) imidazole (Tai H) are reported for the first time. Monomolecular layer of TaiH is formed on silver nano particles (SNPs) at a concentration of 4.93×10-6 M in Ag-sol. TaiH molecules are adsorbed on to SNPs the through the imidazole N atom and undergo trans-to-cis dark isomerization as evidenced by the appearance of signature cis-peaks and the UV-Vis absorption spectra. Stable non-aggregated SNPs are resulted by the interaction of TaiH and the particle size is significantly reduced. DFT calculations based on single Ag-atom model are in excellent agreement with experimental observations.

Climato-environmental influence on breeding phenology of native catfishes in River Ganga and modeling species response to climatic variability for their conservation.

The main objectives of the present study were to quantify the environmental, especially temperature and rainfall, effects on breeding phenology of selected catfish species and to predict changes in breeding phenology of the selected species in relation to climatic variability for the Ganga River Basin. The study showed that changes in rainfall pattern may have the most profound effect on gonad maturation and breeding of Mystus tengara and Mystus cavasius followed by the effect of increased water temperature due to rising air temperature. Indication of region-specific adaptation was noticed in reproductive phenology of Eutropiichthys vacha based on local trends of warming climate. The other habitat parameters, such as dissolved oxygen, alkalinity, nitrate, and phosphate, were correlated with gonad maturity and spawning. Climatic variability may bring region-specific changes in breeding phenology of fish species in the Ganga River. Under a warming climate, changes in precipitation pattern manifested into riverine flow pulse may be the key driver in dictating breeding phenology. Our study indicates E. vacha as a climate sensitive species that may be selected as a target species for climate change impact studies.

Benchmarking pre-spawning fitness, climate preferendum of some catfishes from river Ganga and its proposed utility in climate research.

The concept of threshold condition factor (Fulton), beyond which more than 50% of the female fish population may attain readiness for spawning coined as pre-spawning fitness (K spawn50), has been proposed in the present article and has been estimated by applying the non-parametric Kaplan-Meier method for fitting survival function. A binary coding strategy of gonadal maturity stages was used to classify whether a female fish is "ready to spawn" or not. The proposed K spawn50 has been generated for female Mystus tengara (1.13-1.21 units), M. cavasius (0.846-0.945 units), and Eutropiichthys vacha (0.716-0.799 units). Information on the range of egg parameters (fecundity, egg weight, egg diameter) expected at the pre-spawning stage was also generated. Additional information on species-specific thermal and precipitation window (climate preferendum) within which K spawn50 is attained was also generated through the LOESS smoothing technique. Water temperatures between 31 and 36 °C (M. tengara), 30 and 32 °C (M. cavasius), and 29.5 and 31 °C (E. vacha) and monthly rainfall between 200 and 325 mm (M. tengara), > 250 mm (M. cavasius), and around 50 mm and between 350 and 850 mm (E. vacha) were found to be optimum for attainment of K spawn50. The importance of parameterization and benchmarking of K spawn50 in addition to other conventional reproductive biology parameters has been discussed in the present article. The purposes of the present study were fulfilled by generating baseline information and similar information may be generated for other species replicating the innovative methodology used in this study.

Reproductive biology of Indian Silurid catfish Ompok pabda in river Gomti.

Reproductive biology of an Indian Silurid catfish, Ompok pabda were determined in a moderately impacted tropical River Gomti in India. Samples offish were collected monthly between May 2008 to April 2009 in two zones; upstream site at Mishrikh, Sitapur and midstream site at Lucknow. Length at first maturity calculated through maturity curve was 12.9 cm (males) and 13.9 cm (females) in total length. The gonado-somatic index indicated that spawning generally occurred between June - September and sex ratio showed a predominance of females, representing 54.3% of the total sample. The fecundity was found to vary from 2460 to 5986 nos, with mean of 4330 +/- 799 for the fish with total length of 11.5-20.0 cm. The relationship of fecundity with total length and total body weight of the fish was Log F = 0.374 Log L+ 0.37, r2 = 0.999 and Log F = 0.337 Log W-0.40, r2 = 0.966. The well defined traits of O. pabda from the Ganges basin provide basic information which can be further used for species conservation planning.