RESUMEN
An LC/MS method was developed and validated for the quantitative determination and confirmation of antimycin-A (ANT-A) in water from lakes or streams. Three different water sample volumes (25, 50, and 250 mL) were evaluated. ANT-A was stabilized in the field by immediately extracting it from water into anhydrous acetone using SPE. The stabilized concentrated samples were then transported to a laboratory and analyzed by LC/MS using negative electrospray ionization. The method was determined to have adequate accuracy (78 to 113% recovery), precision (0.77 to 7.5% RSD with samples > or = 500 ng/L and 4.8 to 17% RSD with samples < or = 100 ng/L), linearity, and robustness over an LOQ range from 8 to 51 600 ng/L.
Asunto(s)
Antimicina A/química , Cromatografía Liquida/métodos , Espectrometría de Masas/métodos , Contaminantes Químicos del Agua/química , Agua/química , Estructura Molecular , Reproducibilidad de los Resultados , Sensibilidad y EspecificidadRESUMEN
Canada's population is increasing, and aging. These demographic patterns are accompanied by a growing awareness and evidence base of the benefits to society of leading a healthy and active life. The Canadian Academy of Lifestyle Medicine (CALM) was created to fill a knowledge gap in the Canadian public: how to lead a healthier and more active life. CALM aimed to address these challenges by confronting the lack of assistance modern medicine provides. As a diverse collaborative network using a lifestyle medicine philosophy, CALM's objective was to generate discussions and examine lifestyle medicine approaches to improving overall health and well-being for Canadians. CALM aimed to engage patients whose access to health care is through a physician and provide an innovative platform to support care and healthy decision making. Despite perceived widespread support, intense planning, and extensive development, CALM was slow to gain traction and realize its full potential. This article describes the experiences and lessons learned in creating CALM from the perspective of the leadership team. Although most CALM activities have ceased, virtual space and social media remain active so too does the work of the leadership team, striving to enable Canadians to develop behaviors that will improve their lifestyle, and their overall well-being.
RESUMEN
A flow-through, continuous exposure test system was developed to expose Daphnia magna to an unstable compound. 35% Perox-Aid is a specially formulated hydrogen peroxide (a highly oxidative chemical) product approved for use in U.S. aquaculture and therefore has the potential to be released from aquaculture facilities and pose a risk to aquatic invertebrates. The study objective was to assess the effects of 35% Perox-Aid on an aquatic invertebrate by evaluating the survival, growth, production, and gender ratio of progeny from a representative aquatic invertebrate continuously exposed to 35% Perox-Aid. The study design consisted of 6 treatment groups (10 test chambers each) with target hydrogen peroxide concentrations of 0.0, 0.32, 0.63, 1.25, 2.5, and 5.0 mg L(-1). The study was initiated with <24-h-old Daphnia (1 daphnid per chamber) that were exposed to hydrogen peroxide for 21 days. Hydrogen peroxide concentrations < or =1.25 mg L(-1) had no significant effect on Daphnia time to death compared to controls and no significant effect on the time to first brood production and the number of broods produced. Concentrations < or =0.63 mg L(-1) had no significant effect on the total number of young produced. Concentrations > or =0.32 mg L(-1) had a negative effect on Daphnia growth. Hydrogen peroxide had no significant effect on the gender ratio of young produced. All second generation Daphnia were female. A continuous discharge of hydrogen peroxide into aquatic ecosystems is not likely to affect cladocerans if the concentration is maintained at < or =0.63 mg L(-1) for less than 21 days.
Asunto(s)
Daphnia/efectos de los fármacos , Peróxido de Hidrógeno/toxicidad , Contaminantes Químicos del Agua/toxicidad , Animales , Acuicultura , Daphnia/fisiología , Femenino , Reproducción/efectos de los fármacos , Pruebas de Toxicidad Crónica/métodosRESUMEN
A simple method was developed for determining florfenicol concentration in a small volume (250 micro l) of plasma from five phylogenetically diverse species of freshwater fish. Florfenicol was isolated from the plasma matrix through C(18) solid-phase extraction and quantified by reversed-phase high-performance liquid chromatography with UV detection. The accuracy (84-104%), precision (%RSDAsunto(s)
Cromatografía Líquida de Alta Presión/métodos
, Tianfenicol/análogos & derivados
, Tianfenicol/sangre
, Animales
, Peces
, Reproducibilidad de los Resultados
, Especificidad de la Especie
, Espectrofotometría Ultravioleta
, Tianfenicol/farmacocinética
RESUMEN
Chloramine-T is a disinfectant being developed as a treatment for bacterial gill disease in cultured fish. As part of the drug approval process, a method is required for the confirmation of chloramine-T residues in edible fish tissue. The marker residue that will be used to determine the depletion of chloramine-T residues from the edible tissue of treated fish is para-toluenesulfonamide (p-TSA), a metabolite of chloramine-T. The development and validation of a procedure for the confirmation of p-TSA is described. Homogenized fish tissue is dried by mixing with anhydrous sodium sulfate, and the mixture is extracted with methylene chloride. The extract is passed through a silica gel solid-phase extraction column, from which p-TSA is subsequently eluted with acetonitrile. The acetonitrile extract is evaporated, and the oily residue is dissolved in hexane. The hexane solution is shaken with fresh acetonitrile. The acetonitrile solution is evaporated and the residue is redissolved in dilute potassium hydroxide solution. The aqueous solution is extracted with methylene chloride to further remove more of the fat co-extractive. The aqueous solution is reacted with pentafluorobenzyl bromide in presence of tetrabutylammonium hydrogensulfate. The resulting di-(pentafluorobenzyl) derivative of p-TSA is analyzed by gas chromatography/mass spectrometry. This method permits the confirmation of p-TSA in edible fish tissue at 20 ppb.
Asunto(s)
Residuos de Medicamentos/análisis , Carne/análisis , Sulfonamidas/análisis , Tolueno/análogos & derivados , Tolueno/análisis , Animales , Peces , Cromatografía de Gases y Espectrometría de MasasAsunto(s)
Enfermería de Urgencia , Servicio de Urgencia en Hospital , Capacitación en Servicio , Enfermeras Practicantes/provisión & distribución , Personal de Enfermería en Hospital/provisión & distribución , Educación de Postgrado en Enfermería , Enfermería de Urgencia/educación , Humanos , Perfil Laboral , Mentores , Michigan , Moral , Enfermeras Practicantes/educación , Rol de la Enfermera , Investigación en Administración de Enfermería , Personal de Enfermería en Hospital/educación , Grupo de Atención al Paciente , Desarrollo de Programa , Recursos HumanosAsunto(s)
Comportamiento del Consumidor , Sistemas de Información , Programas Controlados de Atención en Salud/tendencias , Modelos Organizacionales , Técnicas de Apoyo para la Decisión , Planes de Asistencia Médica para Empleados , Humanos , Programas Controlados de Atención en Salud/organización & administración , Innovación Organizacional , Estados UnidosRESUMEN
The point of departure of this study is the derailment of a train in Mississauga, Ontario, 10 Nov 1979 and the subsequent events. The purpose was not to investigate how and why the accident happened, but rather, to examine its consequences in order to suggest ways in which a society can be better prepared to prevent, and to respond to future emergencies.four major objectives were set for the study: 1) To record accurately, and in detail, the events following the derailment; 2) To analyze the response to the emergency of a) Government; b) Voluntary agencies; c) Private sector; d) The public; 3) To assess the soecial, health and economic cost of the evacuation; 4) To examine aspects of the institutional framework sur rounding the emergency, including: a) Emergency planning, b) Risk assessment; c) Insurance;d) Compensation. Some of the conclusions reached lead to suggestions for improving organisational responses to emergencies. The detailed information obtained from public surveys provides important data for modelling how the public will respond in an evacuation