[The indices of cellular-vascular, coagulatory haemostasis and fibrinolysis in arterial hypertension of patients of different age groups].

The contrastive analysis of the indices of cellular and vascular, coagulatory haemostasis and fibrinolysis in arterial hypertension (AH) was held among the patients of different age groups. 69 patients with AH were researched. The average age of afflicted people in the first blockette is 50,9+/-0,7 years, in the second blockette - 70,7+/-1,5. In order to evaluate the condition of haemostasis the afflicted people were researched in the following indices: amount of thrombocytes, hemolysate-aggregative test (HAT), leukocytic-thrombocytic aggregation (LTA), activity of Villebrand factor (FV), inactivation index of thrombin (IIT), activated partial thromboplastin time (APTT), prothrombin index (PTI), fibrinogen, antithrombin III (AIII), Hageman-dependent fibrinolysis (HDF), lupous anticoagulants (LA), autocoagulatory test (ACT). All the examined patients with AH showed considerable abnormalities in cellular and vascular and coagulatory links of haemostasis and the system of fibrinolysis. It is observed, that aged afflected people with AH have progressive hyperaggregation of thrombocytes, the activity of Villebrand factor increases, the rise of coagulatory link activity of haemostasis and the exhaustion of anticoagulants' products is more considerable, the subsequent fibrinolysis depression is observed, but hyperfibrinogenemia accrues. The received results show the heterogeneity of AH in different age groups.

[Association of age-dependent magnetosensitivity dynamics with arterial hypertension].

The purpose of the research was to define magneto sensitivity degree in males depending on age and presence or absence of arterial hypertension. It has been shown, that at aging in the examined groups the number of males possessing high magneto sensitivity degree according to hypotensive type of cardiovascular reaction to magnetic test is decreasing (30,2% in children and up to 0% in males of senile age) and increasing (2,2 up to 36,0%, correspondingly) - according to hypertensive type. In the group of middle aged males with arterial hypertension the number of high magneto sensitive persons on hypertensive type was 5 times higher as compared to the group of practically healthy males of similar age. The results obtained demonstrate that at aging reactions of cardiovascular system on external physical impacts essentially change, dependence of an organism on changes of the geomagnetic field amplifies and probability of risk of hypertonic crisis development during the fluctuations of the Earth magnetic field increases.

[Effect of cytoprotection on the oxidative processes and endothelial function in elderly patients with ischemic heart disease].

The aim of the study was to investigate the effects of cytoprotectional drug Mildronate ("Grindex", Latvia) on some parameters of oxidative processes and endothelial function in elderly patients with coronary heart disease (CHD). One hundred seventeen elderly CHD patients were included into controlled study. The criteria to include the patients into the study were: men and women upwards 60 years old with CHD, with heart failure FC II or III (in accord, NYHA classification), with arterial hypertension (AH), without diabetes mellitus. All patients were randomized into 2 groups: 1) 67 patients 75.4 +/- 0.5 years old were treated with Mildronate 500 mg/day against traditional basal therapy during 12 weeks (the main group) and 2) 50 patients 74.0 +/- 0.6 years old were treated only traditional basal therapy during 12 weeks (the compare group). The blood lipid profile parameters, such as total cholesterol (CH), triglycerides (TG), low density lipoprotein cholesterol (LDL-CH), high density lipoprotein CH (HDL-CH), LDL antioxidant potential (concentration of alpha-tocopherol and retinol in LDL), initial level of lipid peroxidation (LPO) products in LDL, LDL resistance to oxidation in vitro and the blood level of NO metabolites were evaluated before and after 4 and 12 weeks of the study. Effect of Mildronate on the blood lipid profile parameters in elderly CHD patients was not revealed. The initial level of LPO products in LDL was decreased and LDL resistance to oxidation in vitro was increased in main group patients after 12 weeks of study in comparison with the same parameters before the study and with compare group patients (p < 0.05). The blood level of NO metabolites was 1.5-fold higher in main group patients after 12 weeks of study in comparison with the same parameters before the study and with compare group patients (p < 0.05). Thus, in elderly CHD patients the several antiatherogenic effects of Mildronate, such as the decreasing activity of LPO processes in LDL and the increasing synthesis/secretion of blood NO, were revealed.

[Blood lipids, oxidative resistance of low density lipoproteins, concentration of fat-soluble antioxidants in older people and longevity in Novosibirsk]. / Lipidy krovi, okislitel'naia rezistentnost' lipoprotinov nizkoi plotnosti, kontsentratsii zhirorastvorimykh antioksidantov u liudei starcheskogo vozrasta i dolgozhitelei g. Novosibirska.

The aim of the study was to evaluate the blood lipid profile, oxidative-antioxidative status of low density lipoproteins (LDL) in old people and long-living people in Novosibirsk. Eighty nine persons from Novosibirsk Veteran House (23 men and 66 women) at the age of 83-105 (average 92.0 +/- 4.0 years old) were included into the study. They were divided into two groups. The group of old people included 12 person at the age of 75-89. The group of long-living people included 77 person at the age of 90-105. The control group consisted of 90 healthy subjects at the age of 36-59. TCH, CH-HDL and TG were measured using biochemical enzyme methods. LDL were isolated from serum by heparin-precipitation method. The initial level of peroxidation lipid (POL) products in LDL and oxidative resistance of precipitated LDL in vitro were evaluated. Concentrations of liposoluble antioxidants (a-tocopherol and retinol) in precipitated LDL were measured by spectro-fluorimetrical methods. The level of CH-HDL was low (< 40 mg/dl) in old and long-living people. In old people the initial level of POL products in LDL was higher in comparison with healthy subjects of control group (p < 0.0001) and long-living people (p < 0.01). In old people and long-living people the oxidative resistance of LDL in vitro was lower (p < 0.0001) in comparison with control group. Moreover, in long-living people the oxidative resistance of LDL in vitro was also lower (p < 0.01) in comparison with old people. The concentration of retinol in LDL was higher only in long-living people compared to the subjects of control group. The concentration of a-tocopherol in LDL was lower in old people and in long-living people (p < 0.05) in comparison with the subjects of control group.

[Polymorphism of the gene of angiotensin-converting enzyme and apolipoprotein E gene in long-livers of Novosibirsk city]. / Polimorfizm gena angiotensin-prevrashchaiushchego fermenta i gena apolipoproteina E u dolgozhitelei goroda Novosibirska.

The levels of polymorphism of genes of angiotensin converting enzyme (ACE) and apolipoprotein E (Apo E) were studied in elderly and long-living people in Novosibirsk. The results of the study in the investigated group (97 subjects) were compared with polymorphism of these genes in Novosibirsk population group aged 25-64 who were investigated in MONICA Project survey and had DNA data base formed. Frequency of D/D genotype among senile and long-living men was 5.9%. It is 5 times lower than in men 55-64 years of age (p = 0.04). Similar decrease of this gene frequency was also found in women of the same age. In men older than 83 years of age 4 times lowering of 3/4 genotype of Apo E gene and 2 times increasing of frequency of 2/3 genotype were revealed when comprising frequency of these genotypes in people of middle age. In subjects of senile age and long-livers of both sexes genotype 4/4 was not revealed. Lipid levels were more favorable in women with genotype 2/3 of Apo E gene (comparatively lower mean level of total cholesterol and higher level of HDL cholesterol) if compared with genotypes 3/3 and 3/4.

[Functional polymorphism of p53 and CCR5 genes in the long-lived of the Siberian region]. / Funktsional'nyi polimorfizm genov p53 i CCR5 u dolgozhitelei Sibirskogo regiona.

The P53 protein is a key regulator of modified-cell apoptosis. The functional oligonucleotide polymorphism of the p53 gene causes the substitution of arginine (Arg) for praline (Pro) in the codon 72. A reduced apoptotic activity of p53 and, as a consequence, development of oncology pathology is associated with the above polymorphism. CCR5 is a compound transmembrane receptor-protein, which apart from chemokines, binds with some molecules and is a coreceptor for HIV-1. 32 bp deletion within the CCR5 encoding region results in the loss of the protein's receptor function. It has been demonstrated that the transmission of the "external" (in respect to cell) stimulus, via the CCR5 system, induces expression of the p53 gene and initiates apoptosis. Allele variants and p53 and CCR5 genotypes (separately and in combinations) were investigated, within the present case study, for 131 long-livers from Novosibirsk and Tyumen Regions. A trend was detected towards accumulation of the p53 Pro alleles in association with the CCR5del32 allele in the study group, which, as the authors believe, can enhance the genome resistance to variable factors that cut the life span.

[Effect of amiodarone on autonomic status and its efficacy in the treatment of different variants of paroxysmal atrial fibrillation]. / Vliianie amiodarona na vegetativnyi status i ego éffektivnost' v lechenii raznykh variantov paroksizmal'noi fibrilliatsii predserdii.

Association of autonomic status and efficacy of therapy with amiodarone was studied in 90 patients with paroxysmal atrial fibrillation of ischemic (n=45, mean age 62 years) and nonischemic (n=45, mean age 38 years) origin. Autonomic status was assessed by measurement of heart rate variability. Electrophysiological and echocardiographical studies were also conducted before and after 4 weeks of amiodarone therapy. Treatment with amiodarone was accompanied by substantial lowering of the tone of autonomic nervous system in patients with adrenergic type of paroxysmal atrial fibrillation while there was no dynamics of parasympathetic tone in patients with vagal or mixed types. Positive association between amiodarone therapy and decrease of left atrial dimension and improvement of left ventricular contractility was also observed. It was concluded that the use of amiodarone was justified in adrenergic but not in vagal or mixed variants of paroxysmal atrial fibrillation.

[Determination of myoglobin concentration in blood in the diagnosis of unstable (progressive) angina pectoris]. / Opredelenie kontsentratsii mioglobina v krovi v diagnostike nestabil'noi (progressiruiushchei) stenokardii.

A survey of 180 patients with unstable angina pectoris has demonstrated that transient hypermyoglobinemia detected in 58% of patients is a more informative parameter than those obtained with electrographic and enzyme immunoassay used in the diagnosis of focal alterations in the heart muscle in the disease. Measurement of serum myoglobin levels by rapid radioimmunoassay in these patients is of predictive value as it allows a group of subjects at high risk for myocardial infarction to be identified.

[Comparative analysis of parameters of corrected and uncorrected QT-interval dispersion of magneto-, electrocardiography and isomagnetic maps in patients with ischemic heart disease]. / Sravnitel'nyi analiz parametrov korrigirovannoi i nekorrigirovanno dispersii intervala Q-T magnito-, élektrokardiografii i izomagnitnykh kart u bol'nykh ishemicheskoi bolezn'iu serdtsa.

AIM: To compare possibilities of magnetocardiography (MCG) and electrocardiography for assessment of regional dispersion of ventricular recovery time using parameters of corrected and uncorrected QT-interval dispersion (DQTs and DQT). MATERIAL: Twenty three patients with class II angina pectoris including 11 patients with history of myocardial infarction (MI) and 13 practically healthy subjects. RESULTS: Mean DQT and DQTc were significantly higher (p<0.005) in patients than in healthy subjects according to both techniques. Values of DQT and DQTc obtained by MCG were higher in patients with history of MI compared with those without MI (p=0.006 and 0.02, respectively). There was a significant positive correlation between age and DQT and DQTc determined by electrocardiography. Mean number of T-wave dipoles was significantly higher in patients than in healthy subjects. Substantial positive correlation was found between number of T-wave dipoles on isomagnetic maps and age in both patients and healthy people. CONCLUSION: The method of MCG gave supplementary information on the state of ventricular depolarization in patients with ischemic heart disease.

[Cardiovascular function and reserve potentials in chronic alcoholism patients]. / Funktsional'noe sostoianie i rezernye vozmozhnosti serdechno-sosudistoi sistemy u bol'nykh khronicheskim alkogolizmom.

In examination of 68 patients with chronic alcoholism some changes in ECG at rest were recorded in 14% of the cases. The time and physical exercise threshold as well as the volume of work were much lower in the alcoholic patients than in healthy persons. These parameters were in reverse correlation with a time-period of alcohol consumption. The elongation of an electric ventricular systole in alcoholic patients was more frequently revealed at the height of bicycle ergometry testing, being in reverse correlation with a "double product" reflecting indirectly oxygen consumption by the myocardium. A conclusion was made of the reduction of the potentialities of the cardiovascular system of alcoholic patients and of a necessity of graded physical exercise for their assessment.

[The diagnostic potentials of magnetocardiography in the combined examination of patients with cardiomyopathies]. / Diagnosticheskie vozmozhnosti magnitokardiografii pri kompleksnom obsledovanii bol'nykh kardiomiopatiiami.

Diagnostic potential of magnetocardiography (MCG) was elucidated in comparison to ECG and echo-CG in 8 patients with cardiomyopathy (CMP) versus 28 healthy males. Elements of normal MCG curve were characterized in relation to incidence rate. Changes in MCG curve were considered with regard to hypertrophy of basal interventricular septum. In left ventricular hypertrophy MCG showed shifts corresponding to the degree of ventricular dilation. MCG diagnostic opportunities are shown in detection of right ventricular hypertrophy in the presence of the right His bundle blockade in patients with dilated CMP.

[Galstena efficacy in patients with biliary dyskinesia, chronic cholecystitis and cholelithiasis]. / "Galstena" u patsientov s diskineziei zhelchnovyvodiashchikh putei, khronicheskim kholetsistitom i zhelchno-kamennoi bolezn'iu.

The response of inflammation, bile secretion, lipid metabolism, LDL sensitivity to oxidation was studied in patients with bile duct dyskinesia (BDD), chronic cholecystitis (CC) and cholelithiasis (CL) before and after a course of therapy with galstena. The treatment improved general condition in 90% of the patients, dyspeptic symptoms relieved or stopped. Galstena relieved general or local inflammation, reduced mean levels of total cholesterol and LDL cholesterol, raised HDL cholesterol. In patients with BDD and cholelithiasis galstena did not change normal sensitivity of LDL to oxidation. A galstena course in BDD and CC patients significantly diminished bile lithogenicity and improved gall bladder contractility.

[Magnetocardiographic studies in the clinical diagnosis of heart diseases]. / Magnitokardiograficheskie issledovaniia v klinicheskoi diagnostike serdechnykh patologii.

The paper provides evidence for the diagnostic value of magnetocardiography employed in the clinical diagnosis of heart diseases. It concludes that it may be used in mass surveys of the general population.

[Magnito-, electro- and echocardiography in detecting symptoms of the "hypertensive heart"]. / Magnito-, élektro- i ekhokardiograficheskie metody v vyiavlenii priznakov "gipertonicheskogo serdtsa".

The study compared diagnostic potential of magnetocardiography (MCG), electrocardiography (ECG) and echocardiography (echo-CG) in 18 patients with arterial hypertension (AH). 32 healthy males served as control. Elements of MCG from normal subjects have been analyzed morphologically in 36 points of precordial leads. Left ventricular hypertrophy was registered at echo-CG, MCG, ECG in 11 (61%), 16 (84%) and 7 (34%) of the AH patients, respectively. Left atrial hypertrophy was discovered primarily by echo-CG and MCG. Defects in ventricular repolarization were recorded by MCg in 7 (39%) patients basing on MCG, echo-CG and rarely ECG signs of left ventricular hypertrophy. MCG is recommended as an effective tool in diagnosis of "hypertensive heart".

[Method of isolating carbamazepine from cadaveric material and its determination by fluorescent polarization immunoassay]. / Metodika izolirovaniia karbamazepina iz trupnogo materiala i opredeleniia ego metodom fliuorestsentno-poliarizatsionnogo immunoanaliza.

Optimal conditions for carbamazepine isolation from cadaveric liver have been determined. The authors recommend the material infusion with alcohol acidified with sulfuric acid to pH 1 three times for an hour. Conditions for measuring the isolated drug quantity have been developed. Fluorescent polarization immunoanalysis in the TDX system has been used for the first time. Carbamazepine detection limit for 50 g of the liver was found to be 2.5 x 10(-7) g.