Changes in soil properties during iron mining and in rehabilitating minelands in the Eastern Amazon.

Open-cast iron mining causes drastic disturbances in soil properties. Recovery of soil chemical and physical properties is essential for successful revegetation and landscape rehabilitation. To identify changes in soil properties during the mining and revegetation process, soil samples were collected from undisturbed sites represented by forest and ferriferous savannas stocking above iron outcrops, called "cangas," in open-pit benches, and in rehabilitation chronosequences of iron waste piles in the Carajás Mineral Province (CMP), Eastern Amazon, Brazil. The samples were analyzed for chemical and physical properties. Our results showed that iron mining operations resulted in significant alteration of the chemical soil properties when forest and canga vegetation are suppressed to form open-pit benches or waste piles in the CMP. Mining substrates showed lower contents of soil organic matter (SOM) and nutrients than undisturbed areas of forests and cangas. In order to achieve the success of revegetation, nutrients have been added prior to plant establishment. We have demonstrated how soil fertility changes along with mineland rehabilitation, and the variation among chronosequence was attributable mainly due to contents of SOM, K, and B in the soil. The slight improvement of SOM found in rehabilitated waste piles reinforces the notion that recovery of soil quality can be a slow process in iron minelands in the CMP.

Human infection with Pseudoterranova cattani by ingestion of "ceviche" in Buenos Aires, Argentina.

Anisakidosis is an infection caused by larval nematodes that belong to several genera within the family Anisakidae. Anisakidosis has about 20000 cases reported to date, the vast majority (90%) in Japan. Usually, human anisakiosis is more common than human pseudoterranovosis in Japan and Europe, although in North America Pseudoterranova spp. is the more frequent. Cases of human pseudoterranovosis have been reported from Chile and Peru. We here report one of the few cases of human infection by Pseudoterranova cattani by consumption of "ceviche" in Buenos Aires, Argentina.

Influence of Mechanical and Chemical Degradation in the Surface Roughness, Gloss, and Color of Microhybrid Composites.

AIM: The aim of this study was to investigate the association of different degradations on the roughness, gloss, and color changes of microhybrid composites. MATERIALS AND METHODS: Ten specimens were prepared for Charisma, Amelogen Plus, Point 4, and Opallis resins. Surfaces were polished and baseline measurements of roughness, gloss, and color were recorded. Specimens were then submitted to chemical and mechanical challenges, and the specimens were reevaluated. Roughness and gloss were analyzed by Kruskal -Wallis and Dunn's test (p < 0.05). Color change (ΔE) was analyzed by one-way analysis of variance and Tukey's tests (p < 0.05). The initial and final data were compared using the Wilcoxon test (p < 0.05). Spearman test checked the correlation between the roughness and gloss (p < 0.05). RESULTS: Regarding surface roughness and gloss, there was no difference between composites before challenges. However, all composites showed a significant increase of roughness after challenges, with highest values for Charisma. The gloss was influenced by challenges, evidencing the best gloss for Point 4. Charisma showed the highest value of color change. There was no correlation between surface roughness and gloss for the initial analysis, and after the challenges. CONCLUSION: Composites were influenced by association of challenges, and Charisma showed the highest changes for roughness, gloss, and color. CLINICAL SIGNIFICANCE: The type of composite resin influenced the properties of materials, which are surface roughness, gloss, and color change. The dentist should be aware of the performance of different brands, to choose the correct required composite resin for each type of patient or region to be restored.

[Assessment of transmammary transmission of Trichinella patagoniensis in BALB/c mice]. / Evaluación de la transmisión galactógena de Trichinella patagoniensis en ratones BALB/c.

Since 1916 to date, it has been suspected that vertical transmission of parasites from the genus Trichinella could occur in pregnant or lactating women during the parenteral phase of infection. The aim of the present study was to evaluate the transmammary transmission of T. patagoniensis in BALB/c mice. Twenty 7-week-old BALB/c mice were distributed into two groups of 10 individuals each, depending on the time of gestation when they were infected, 15 or 18 days after detection of the vaginal plug. Each group was subdivided into two subgroups of 5 mice each, which were given an oral dose of 100 or 500 infective larvae respectively. Euthanasia and subsequent artificial digestion was performed in the pups and the dams. No T. patagoniensis L1 larvae were found in any of the offsprings analyzed. The observed results suggest that vertical transmission of T. patagoniensis would not be possible in BALB/c mice.

Bioaccumulation and effects of lanthanum on growth and mitotic index in soybean plants.

Rare earth elements such as lanthanum (La) have been used as agricultural inputs in some countries in order to enhance yield and improve crop quality. However, little is known about the effect of La on the growth and structure of soybean, which is an important food and feed crop worldwide. In this study, bioaccumulation of La and its effects on the growth and mitotic index of soybean was evaluated. Soybean plants were exposed to increasing concentrations of La (0, 5, 10, 20, 40, 80, and 160 µM) in nutrient solution for 28 days. Plant response to La was evaluated in terms of plant growth, nutritional characteristics, photosynthetic rate, chlorophyll content, mitotic index, modifications in the ultrastructure of roots and leaves, and La mapping in root and shoot tissues. The results showed that the roots of soybean plants can accumulate sixty-fold more La than shoots. La deposition occurred mainly in cell walls and in crystals dispersed in the root cortex and in the mesophyll. When La was applied, it resulted in increased contents of some essential nutrients (i.e., Ca, P, K, and Mn), while Cu and Fe levels decreased. Moreover, low La concentrations stimulated the photosynthetic rate and total chlorophyll content and lead to a higher incidence of binucleate cells, resulting in a slight increase in roots and shoot biomass. At higher La levels, soybean growth was reduced. This was caused by ultrastructural modifications in the cell wall, thylakoids and chloroplasts, and the appearance of c-metaphases.

Lead tolerance of water hyacinth (Eichhornia crassipes Mart. - Pontederiaceae) as defined by anatomical and physiological traits.

This study aimed at verifying the lead tolerance of water hyacinth and at looking at consequent anatomical and physiological modifications. Water hyacinth plants were grown on nutrient solutions with five different lead concentrations: 0.00, 0.50, 1.00, 2.00 and 4.00 mg L-1 by 20 days. Photosynthesis, transpiration, stomatal conductance and the Ci/Ca rate were measured at the end of 15 days of experiment. At the end of the experiment, the anatomical modifications in the roots and leaves, and the activity of antioxidant system enzymes, were evaluated. Photosynthetic and Ci/Ca rates were both increased under all lead treatments. Leaf anatomy did not exhibit any evidence of toxicity effects, but showed modifications of the stomata and in the thickness of the palisade and spongy parenchyma in the presence of lead. Likewise, root anatomy did not exhibit any toxicity effects, but the xylem and phloem exhibited favorable modifications as well as increased apoplastic barriers. All antioxidant system enzymes exhibited increased activity in the leaves, and some modifications in roots, in the presence of lead. It is likely, therefore, that water hyacinth tolerance to lead is related to anatomical and physiological modifications such as increased photosynthesis and enhanced anatomical capacity for CO2 assimilation and water conductance.

Copepod distribution and production in a Mid-Atlantic Ridge archipelago.

The Saint Peter and Saint Paul Archipelago (SPSPA) are located close to the Equator in the Atlantic Ocean. The aim of this study was to assess the spatial variations in the copepod community abundance, and the biomass and production patterns of the three most abundant calanoid species in the SPSPA. Plankton samples were collected with a 300 µm mesh size net along four transects (north, east, south and west of the SPSPA), with four stations plotted in each transect. All transects exhibited a tendency toward a decrease in copepod density with increasing distance from the SPSPA, statistically proved in the North. Density varied from 3.33 to 182.18 ind.m-3, and differences were also found between the first perimeter (first circular distance band) and the others. The total biomass varied from 15.25 to 524.50 10-3 mg C m-3 and production from 1.19 to 22.04 10-3 mg C m-3d-1. The biomass and production of Undinula vulgaris (Dana, 1849), Acrocalanus longicornis Giesbrecht, 1888 and Calocalanus pavo (Dana, 1849) showed differences between some transects. A trend of declining biodiversity and production with increasing distance from archipelago was observed, suggesting that even small features like the SPSPA can affect the copepod community in tropical oligotrophic oceanic areas.

Integration of land-sharing and land-sparing conservation strategies through regional networking: the Mesoamerican Biological Corridor as a lifeline for carnivores in El Salvador.

Nations with little remaining natural habitat and small extent are challenged when trying to achieve biodiversity targets. We show that the Central American nation of El Salvador cannot viably sustain populations of 87 % of its extant carnivores, especially in the case of large-bodied species with low population densities. Current land-sparing strategies will not suffice; therefore we propose that land-sharing strategies be implemented in tandem with protected areas to expand current conservation efforts via new regional networks. In Central America such a network can be established by linking international protected area systems in a way that implements the existing vision for the Mesoamerican Biological Corridor. Specifically, we propose a re-envisioning of the Mesoamerican Biological Corridor in which land-sharing practices are adopted throughout the agricultural matrix while ensuring formal protection of the remaining natural habitat. Such an integration of land-sparing and land-sharing could result in the creation of an effective network of protected areas, thereby increasing the probability of safeguarding species with populations that overlap national borders.

Urban dog densities reveal environmental inequities in Santiago, Chile.

Dogs can negatively affect the wellbeing of people and nature, but if this changes along a socioeconomic gradient, then social inequity might be at fault. Here, we identify environmental injustice at the city scale by modeling differences in the density of urban dog populations according to varying levels of socioeconomic development across municipalities of Chile's capital, Santiago. Our analysis demonstrates a strong relation between dog density and social inequity, specifically because dog density increases along with poverty, but decreases in municipalities with higher municipal income. We offer specific proposals to ameliorate and reverse this inequity. These results expose another aspect of the impacts people and nature are subjected to by dogs, adding a new social lens to address the dog problem worldwide.

Being with the patient: the use of "clinical evidence," reconsidered.

Our objective was to review the use in psychiatry of data arising from interaction with the patient, here called "clinical evidence." We conducted a clinical and historical review. Data from interactions with patients are increasingly marginalized in psychiatry, even as interactional data have an increasing role elsewhere in healthcare. Recommendations for training, clinical care, and administration are made.

Designing management options to reduce surface runoff and sediment yield with farmers: an experiment in south-western France.

To preserve the quality of surface water, official French regulations require farmers to keep a minimum acreage of grassland, especially bordering rivers. These agro-environmental measures do not account for the circulation of water within the catchment. This paper examines whether it is possible to design with the farmers agri-environmental measures at field and catchment scale to prevent soil erosion and surface water pollution. To support this participatory approach, the hydrology and erosion model STREAM was used for assessing the impact of a spring stormy event on surface runoff and sediment yield with various management scenarios. The study was carried out in collaboration with an agricultural committee in an area of south-western France where erosive runoff has a major impact on the quality of surface water. Two sites (A and B) were chosen with farmers to discuss ways of reducing total surface runoff and sediment yield at each site. The STREAM model was used to assess surface runoff and sediment yield under current cropping pattern at each site and to evaluate management scenarios including grass strips implementation or changes in cropping patterns within the catchment. The results of STREAM simulations were analysed jointly by farmers and researchers. Moreover, the farmers discussed each scenario in terms of its technical and economical feasibility. STREAM simulations showed that a 40 mm spring rainfall with current cropping patterns led to 3116 m3 total water runoff and 335 metric tons of sediment yield at site A, and 3249 m3 and 241 metric tons at site B. Grass strips implementation could reduce runoff for about 40% and sediment yield for about 50% at site A. At site B, grass strips could reduce runoff and sediment yield for more than 50%, but changes in cropping pattern could reduce it almost totally. The simulations led to three main results: (i) grass strips along rivers and ditches prevented soil sediments from entering the surface water but did not reduce soil losses, (ii) crop redistribution within the catchment was as efficient as planting grass strips, and (iii) efficient management of erosive runoff required coordination between all the farmers using the same watershed. This study shown that STREAM model was a useful support for farmers' discussions about how to manage runoff and sediment yield in their fields.

[Contribution of the PCR assay to the diagnosis of Mansonella ozzardi in endemic areas of Argentina]. / Aporte de la técnica de PCR en el diagnóstico de Mansonella ozzardi en zonas endémicas de la Argentina.

Mansonella ozzardi is a tissue-dwelling parasitic nematode, the causative agent of mansonelliasis in almost all Latin American countries. It has been described along the Argentine Yungas region. The microscopic diagnosis can yield false-negative test results at low microfilaremia levels. The aim of this study was to optimize the molecular diagnostic technique and compare it with the Knott's method and standard blood smear procedures (thin blood films and thick smears) in 92 blood samples of individuals from an endemic area. The PCR technique followed by the sequencing of the amplified product yielded 100 % sensitivity compared to the Knott's test, which is considered a reference method. Seven more cases of this parasitosis could only be identified with the molecular technique.

Selenium accumulation in lettuce germplasm.

Selenium (Se) is an essential micronutrient for animals and humans. Increasing Se content in food crops offers an effective approach to reduce the widespread selenium deficiency problem in many parts of the world. In this study, we evaluated 30 diverse accessions of lettuce (Lactuca sativa L.) for their capacity to accumulate Se and their responses to different forms of Se in terms of plant growth, nutritional characteristics, and gene expression. Lettuce accessions responded differently to selenate and selenite treatment, and selenate is superior to selenite in inducing total Se accumulation. At least over twofold change in total Se levels between cultivars with high and low Se content was found. Synergistic relationship between Se and sulfur accumulation was observed in nearly all accessions at the selenate dosage applied. The change in shoot biomass varied between lettuce accessions and the forms of Se used. The growth-stimulated effect by selenate and the growth-inhibited effect by selenite were found to be correlated with the alteration of antioxidant enzyme activities. The different ability of lettuce accessions to accumulate Se following selenate treatment appeared to be associated with an altered expression of genes involved in Se/S uptake and assimilation. Our results provide important information for the effects of different forms of Se on plant growth and metabolism. They will also be of help in selecting and developing better cultivars for Se biofortification in lettuce.

Nitrogen and phosphorus removal using a novel integrated system of natural zeolite and lime.

Nitrogen and phosphorus can cause eutrophication problems in receiving waters. These nutrients can be eliminated by different wastewater treatments but they tend to be costly or require complex operations. With these reasons in mind, this work set out to study the behavior of a novel combined or integrated system with zeolite and lime for the removal of these chemical species. With the integrated treatment, excellent removals-98 % NH(+) (4), 100 % total phosphorus (TP), 79 % chemical oxygen demand (COD), 71 % BOD(5), 99 % of total suspended solids (TSS) and 100 % of fecal coliforms-were obtained. A sludge production of 4.4 % and 4.8 % of the wastewater total volume treated with lime was achieved. The final liquid effluent of the integrated system of natural zeolite and lime had a composition which was suitable for its discharge into sewerage systems and marine or continental waters (according to Chilean regulations), as far as the concentrations of nitrogen, phosphorus and total coliforms were concerned. Validated curves with their corresponding equations were obtained in this study for the removal of ammonia nitrogen and total phosphorus. These equations can theoretically be applied to estimate the removal of both parameters in studies or practical applications undertaken with operating conditions similar to those used in this work.

Native Amazonian Canga Grasses Show Distinct Nitrogen Growth Responses in Iron Mining Substrates.

Native species may have adaptive traits that are advantageous for overcoming the adverse environmental conditions faced during the early stages of mine land rehabilitation. Here, we examined the nitrogen (N) growth responses of two native perennial grasses (Axonopus longispicus and Paspalum cinerascens) from canga in nutrient-poor iron mining substrates. We carried out vegetative propagation and recovered substantial healthy tillers from field-collected tussocks of both species. These tillers were cultivated in mining substrates at increasing N levels. The tillering rates of both species increased with the N application. Nonetheless, only in P. cinerascens did the N application result in significant biomass increase. Such growth gain was a result of changes in leaf pigment, stomatal morphology, gas exchanges, and nutrients absorption that occurred mainly under the low N additions. Reaching optimum growth at 80 mg N dm-3, these plants showed no differences from those in the field. Our study demonstrates that an input of N as fertilizer can differentially improve the growth of native grasses and that P. cinerascens plants are able to deposit high quantities of carbon and protect soil over the seasons, thus, making them promising candidates for restoring nutrient cycling, accelerating the return of other species and ecosystem services.

Association between irrigation thresholds and promotion of soil organic carbon decomposition in sandy soil.

Soil organic carbon (SOC) has a significant effect on the carbon cycle, playing a vital role in environmental services and crop production. Increasing SOC stock is identified as an effective way to improve carbon dioxide sequestration, soil health, and plant productivity. Knowing soil water is one of the primary SOC decomposition driver, periods in the crops growth stages with increased water movement might influence the SOC dynamics. Here, we evaluate the temporal effect of four precision irrigation thresholds ([Formula: see text], [Formula: see text], [Formula: see text], and [Formula: see text] kPa) in potato crop on SOC dynamics using the Partial Least Square algorithm and the Tea Bag Index in a sandy soil under potato production. The difference of SOC decomposition rate between the precision irrigation thresholds is developed in the second quarter of the growing season, between 38 and 53 days after planting. This critical period occurred in a stage of strong vegetative growth and rapid irrigation cycles. The precision irrigation threshold affected the decomposition rate of SOC. A faster decomposition of labile organic carbon was promoted by water excess ([Formula: see text] kPa). The dryer ([Formula: see text], [Formula: see text], and [Formula: see text] kPa) precision irrigation thresholds did not show any differences. The advancement of this knowledge may promote soil health conservation and carbon sequestration in agricultural soil.

Estimation of river flow using CubeSats remote sensing.

River flow characterizes the integrated response from watersheds, so it is essential to quantify to understand the changing water cycle and underpin the sustainable management of freshwaters. However, river gauging stations are in decline with ground-based observation networks shrinking. This study proposes a novel approach of estimating river flows using the Planet CubeSats constellation with the possibility to monitor on a daily basis at the sub-catchment scale through remote sensing. The methodology relates the river discharge to the water area that is extracted from the satellite image analysis. As a testbed, a series of Surface Reflectance PlanetScope images and observed streamflow data in Araguaia River (Brazil) were selected to develop and validate the methodology. The study involved the following steps: (1) survey of measurements of water level and river discharge using in-situ data from gauge-based Conventional Station (CS) and measurements of altimetry using remote data from JASON-2 Virtual Station (JVS); (2) survey of Planet CubeSat images for dates in step 1 and without cloud cover; (3) image preparation including clipping based on different buffer areas and calculation of the Normalized Difference Vegetation Index (NDVI) per image; (4) water bodies areas calculation inside buffers in the Planet CubeSat images; and (5) correlation analysis of CubeSat water bodies areas with JVS and CS data. Significant correlations between the water bodies areas with JVS (R2 = 88.83%) and CS (R2 = 96.49%) were found, indicating that CubeSat images can be used as a CubeSat Virtual Station (CVS) to estimate the river flow. This newly proposed methodology using CubeSats allows for more accurate results than the JVS-based method used by the Brazilian National Water Agency (ANA) at present. Moreover, CVS requires small areas of remote sensing data to estimate with high accuracy the river flow and the height variation of the water in different timeframes. This method can be used to monitor sub-basin scale discharge and to improve water management, particularly in developing countries where the presence of conventional stations is often very limited.

Combining genotype, phenotype, and environmental data to delineate site-adjusted provenance strategies for ecological restoration.

Despite the importance of climate-adjusted provenancing to mitigate the effects of environmental change, climatic considerations alone are insufficient when restoring highly degraded sites. Here we propose a comprehensive landscape genomic approach to assist the restoration of moderately disturbed and highly degraded sites. To illustrate it we employ genomic data sets comprising thousands of single nucleotide polymorphisms from two plant species suitable for the restoration of iron-rich Amazonian Savannas. We first use a subset of neutral loci to assess genetic structure and determine the genetic neighbourhood size. We then identify genotype-phenotype-environment associations, map adaptive genetic variation, and predict adaptive genotypes for restoration sites. Whereas local provenances were found optimal to restore a moderately disturbed site, a mixture of genotypes seemed the most promising strategy to recover a highly degraded mining site. We discuss how our results can help define site-adjusted provenancing strategies, and argue that our methods can be more broadly applied to assist other restoration initiatives.

Evaluation "in situ" of tag formation in dental enamel submitted to microabrasion technique. Effect of two etching times.

The objective of this study was to analyze the formation of resin tags on enamel surfaces submitted or not to enamel microabrasion technique. Thirteen undergraduate dental students received removable acrylic palatal appliances on which four sections from intact premolars were fixed, measuring 4.0 mm x 4.0 mm. Two sections received the application of a microabrasive system and the remaining sections did not receive any surface treatment. The patients were instructed to wear the acrylic palatal appliances for 4 months during the day. After this time, the sections were etched with 37% phosphoric acid for 15 seconds (sections 1 and 2) and 60 seconds (sections 3 and 4), followed by adhesive system and composite resin applications. All the sections were prepared for light microscopy analysis to observe the degree of resin penetration (x400). The results were submitted to a three-way analysis of variance with a significance level of 5%. The difference between groups was verified by the Tukey test, at a significance level of 5%. The results showed that microabraded enamel required a longer period of acid etching for longer resin tags.