U12-dependent intron splicing in plants.

U12-dependent (U12) introns have persisted in the genomes of plants since the ancestral divergence between plants and metazoans. These introns, which are rare, are found in a range of genes that include essential functions in DNA replication and RNA metabolism and are implicated in regulating the expression of their host genes. U12 introns are removed from pre-mRNAs by a U12 intron-specific spliceosome. Although this spliceosome shares many properties with the more abundant U2-dependent (U2) intron spliceosome, four of the five small nuclear RNAs (snRNAs) required for splicing are different and specific for the unique splicing of U12 introns. Evidence in plants so far indicates that splicing signals of plant U12 introns and their splicing machinery are similar to U12 intron splicing in other eukaryotes. In addition to the high conservation of splicing signals, plant U12 introns also retain unique characteristic features of plant U2 introns, such as UA-richness, which suggests a requirement for plant-specific components for both the U2 and U12 splicing reaction. This chapter compares U12 and U2 splicing and reviews what is known about plant U12 introns and their possible role in gene expression.

Functional and psychosocial effects of multimodality limb-sparing therapy in patients with soft tissue sarcomas.

We have documented functional and psychosocial changes in patients with extremity soft tissue sarcomas who have undergone multimodality limb-sparing treatments. In 88 patients, parameters related to economic status, sexual activity, pain, limb function, and global quality of life (QOL) were recorded prior to surgery and every 6 months postoperatively. Changes from the preoperative assessment for every parameter were analyzed in each patient. Six months after surgery, there was a decrease in employment status, sexual activity, and in limb function in a significant number of patients. At 12 months, these decreases were still evident. Despite these changes, global QOL measured by a standardized test showed at least some improvement in a significant proportion of patients at 12 months. These findings highlight the difficulty in defining QOL. It could not be ascertained if radiation therapy and/or chemotherapy were causative factors in specific changes because of the small numbers of patients in each subgroup. However, among 60 patients with high-grade sarcomas, significant wound problems developed in 10 of 33 who received postoperative radiation therapy in combination with adjuvant doxorubicin and cyclophosphamide chemotherapy compared with one of 27 patients who received adjuvant chemotherapy alone (P = .016). Also, among high-grade sarcoma patients with 12-month follow-up, six of 19 patients who received radiation therapy and chemotherapy developed joint contractures compared with zero of 15 patients who received chemotherapy alone (P less than .04). The combination of postoperative radiation therapy and chemotherapy appeared to be associated with significantly more tissue-related injury in patients with high-grade sarcomas compared with chemotherapy alone.

Choriocarcinomatous metaplasia of a metachronous adenocarcinoma of the colon.

A second primary colonic adenocarcinoma developed in a 68-year-old man following resection of a rectal adenocarcinoma. Choriocarcinomatous change was found in the metachronous lesion with liver metastases and elevated beta-human choriogonadotrophin (HCG) serum titres.

The giant hand phenomenon.

The "giant hand" is a form of recognized spatial disorientation. An experienced fighter pilot described the events leading up to ejection from his out-of-control aircraft. Investigation of the mishap found no flight control problems and attributed the accident to the giant hand phenomenon. A survey of tactical aircrews indicated that this form of spatial disorientation is not an unusual occurrence. Past training of aircrew to deal with this problem has been inadequate. Earlier reports of this phenomenon recommended attempting to recover the aircraft by grasping the stick using only the thumb and index finger rather than the entire hand. Further investigation to include simulation of this phenomenon in advanced spatial disorientation trainers should contribute to aviation safety.

A test of thumb and index finger control in overcoming a visual analogue of the giant hand illusion.

Anecdotal evidence suggests that a thumb and index finger grip might facilitate recovery from the manifestation of spatial disorientation known as the giant hand phenomenon. Sixteen pilots volunteered as subjects in an experiment that compared the effectiveness of the thumb and index finger versus the whole hand technique to overcome a visually-induced analogue of the giant hand phenomenon. Thumb and index finger control produced greater stability overall, but did not overcome the specific tracking bias induced by a background visual roll stimulus. Various hypotheses are discussed as to why the thumb and index finger technique was ineffective in the present instance.

East meets West: a comparison of eastern block/western aeromedical practices.

Under the auspices of the European Command (EUCOM) Military-to-Military Exchange Program, the authors participated in 13 trips to visit aeromedical facilities of nine Eastern European nations (Albania, Belarus, Bulgaria, Czech Republic, Hungary, Lithuania, Poland, Romania, and Slovakia). In addition, eight of these Eastern European nations visited United States Air Force (USAF) aeromedical facilities. This article highlights the similarities and differences noted between the USAF and Eastern Europe in the practice of aerospace medicine. Flight surgeons from both Eastern Europe and the USAF address issues such as physiologic stresses of flight (acceleration, hypoxia, etc.) and lifestyle stresses (rest, diet, alcohol, cigarettes, etc.). Eastern European Flight Surgeons do not regularly fly. The Eastern European approach to medical standards and screening for aviation applicants is much stricter and more comprehensive than ours. Several of the nations visited had active research programs at their central aeromedical institute emphasizing aircrew selection and retention standards. With the exception of the Czech Republic, Eastern European nations did not routinely grant waivers for chronic medical conditions such as hypertension in aircrew. Soviet-built aircraft had many unique features such as an outside-in attitude indicator and an auto-recovery system.