Association of fasting glucose with subclinical cerebrovascular disease in older adults without Type 2 diabetes.

AIMS: To examine how fasting glucose and glucose tolerance are related to magnetic resonance imaging-assessed indicators of subclinical cerebrovascular disease and brain atrophy and their variation according to age, sex and education. METHODS: Participants in the present study were 172 healthy, community-dwelling older adults. An oral glucose tolerance test was administered and magnetic resonance imaging performed. Fasting, 2-h, and 2-h area-under-the-curve glucose levels, their associations with subclinical cerebrovascular disease and brain atrophy, and their respective interactions with age, sex and education were examined. RESULTS: A positive association between fasting glucose and subclinical cerebrovascular disease (but not brain atrophy) emerged; this association was more pronounced for participants with < 12 years of education; however, glucose tolerance was not related to subclinical cerebrovascular disease or brain atrophy. CONCLUSIONS: Findings revealed a potential link between fasting glucose levels and the presence of subclinical cerebrovascular disease indicators - white matter hyperintensities and silent brain infarction - in older adults without diabetes and with an education level below high school. Additional research is needed to confirm these associations and to determine the need for interventions aimed at closely monitoring and preventing elevated glucose levels in this population to reduce the prevalence of subclinical cerebrovascular disease.

Pyrene mineralization by Mycobacterium sp. strain KMS in a barley rhizosphere.

To determine whether the soil Mycobacterium isolate KMS would mineralize pyrene under rhizosphere conditions, a microcosm system was established to collect radioactive carbon dioxide released from the labeled polycyclic aromatic hydrocarbon. Microcosms were designed as sealed, flow-through systems that allowed the growth of plants. Experiments were conducted to evaluate mineralization of 14C-labeled pyrene in a sand amended with the polycyclic aromatic hydrocarbons degrading Mycobacterium isolate KMS, barley plants, or barley plants with roots colonized by isolate KMS. Mineralization was quantified by collecting the 14CO2 produced from 14C-labeled pyrene at intervals during the 10-d incubation period. Roots and foliar tissues were examined for 14C incorporation. Mass balances for microcosms were determined through combustion of sand samples and collection and quantification of 14CO2 evolved from radiolabeled pyrene. No pyrene mineralization was observed in the sterile control systems. Greater release of 14CO2 was observed in the system with barley colonized by KMS than in microcosms containing just the bacterium inoculum or sterile barley plants. These findings suggest that phytostimulation of polycyclic aromatic hydrocarbons mineralization could be applied in remediation schemes.

Theory and application of landfarming to remediate polycyclic aromatic hydrocarbons and mineral oil-contaminated sediments; beneficial reuse.

When applying landfarming for the remediation of contaminated soil and sediment, a fraction of the soil-bound contaminant is rapidly degraded; however, a residual concentration may remain, which slowly degrades. Degradation of polycyclic aromatic hydrocarbons (PAHs) and mineral oil can be described using a multi-compartment model and first-order kinetics, in which three degradable fractions are distinguished; (1) rapid, (2) slowly, and (3) very slowly degradable. Using this model populated with data from long-term experiments (initiated in 1990), it is shown that time frames from years to decades can be necessary to clean the soil or sediment to obtain a target below regulatory guidelines. In passive landfarms without active management, three principal potentially limiting factors can be identified: (1) availability of appropriate microorganisms, (2) supply of oxygen for the biodegradation process, and (3) bioavailability of the pollutants to the microorganisms. Bioavailable PAHs and mineral oil are readily biodegradable contaminants under aerobic conditions, and presence and activity of microorganisms are not problems. The other two factors can be limiting and are theoretically described. Using these descriptions, which are in agreement with field experiments of 10 to 15 yr, it is shown if and when optimization of the biodegradation process is an option. Because a long time period is necessary to degrade the slowly and very slowly degradable fractions, passive landfarming should be combined with beneficial use of the land area. Examples include the development of natural environments, use in constructions, growing of biomass for energy production, including biofuels, and use as cover for landfills.

Sustainable land application: an overview.

Man has land-applied societal nonhazardous wastes for centuries as a means of disposal and to improve the soil via the recycling of nutrients and the addition of organic matter. Nonhazardous wastes include a vast array of materials, including manures, biosolids, composts, wastewater effluents, food-processing wastes, industrial by-products; these are collectively referred to herein as residuals. Because of economic restraints and environmental concerns about land-filling and incineration, interest in land application continues to grow. A major lesson that has been learned, however, is that the traditional definition of land application that emphasizes applying residuals to land in a manner that protects human and animal health, safeguards soil and water resources, and maintains long-term ecosystem quality is incomplete unless the earning of public trust in the practices is included. This overview provides an introduction to a subset of papers and posters presented at the conference, "Sustainable Land Application," held in Orlando, FL, in January 2004. The USEPA, USDA, and multiple national and state organizations with interest in, and/or responsibilities for, ensuring the sustainability of the practice sponsored the conference. The overriding conference objectives were to highlight significant developments in land treatment theory and practice, and to identify future research needs to address critical gaps in the knowledge base that must be addressed to ensure sustainable land application of residuals.

Fate of pyrene in contaminated soil amended with alternate electron acceptors.

Creosote-contaminated soil samples from the Libby Ground Water Contamination Superfund Site in Libby, MT, were amended with the potential alternate electron acceptors (AEA) nitrate (KNO3), manganese oxide (MnO2), and amorphous iron oxyhydroxide (FeOOH) and incubated at low oxygen tensions (0-6% O2). The fate of 14C-pyrene was evaluated with respect to the different soil amendments. The fate of 14C from the radiolabeled pyrene with regard to mineralization and bound residue formation within soil humic fractions was not significantly different from controls for the iron and manganese amended soils. Nitrate amendments appeared to stimulate 14C-pyrene mineralization at a level of 170 mg NO3-N kg(-1), and inhibit mineralization at 340 mg NO3-N kg(-1). The stimulatory effect did not appear to be the result of nitrate serving as an electron acceptor. Although AEA amendments did not significantly affect the rate or extent of 14C-pyrene mineralization, results of oxygen-deprived incubations (purged with N2) indicate that AEA may be utilized by the microbial community in the unsaturated contaminated soil system.

Pentachlorophenol and phenanthrene biodegradation in creosote contaminated aquifer material.

Contamination of the subsurface environment at the Libby Superfund Site, Montana, includes polycyclic aromatic hydrocarbons and f1p4achlorophenol due to accidental spills and improper disposal of wood preserving wastes. Biodegradation is a treatment technology gaining wide application in the treatment of hazardous waste sites. A microcosm study was conducted to evaluate the effect of temperature, sampling depth, nutrient addition, and oxygen on the biodegradation potential of phenanthrene and pentachlorophenol in aquifer samples using radiolabeled chemicals. Mineralization of phenanthrene reached 14% but was less than 1% for pentachlorophenol over the 56 day incubation period. Phenanthrene mineralization in microcosms at 10 degrees C was not significantly different from those at 20 degrees C. This may have been due to microbial community acclimation to lower temperatures at the site. Average volatilization was less than 2% for both phenanthrene and pentachlorophenol. After 56 days, most of the radiolabeled chemical was either solvent extractable or soil bound.

Hycrest crested wheatgrass accelerates the degradation of pentachlorophenol in soil.

We investigated the effects of vegetation on the fate of pentachlorophenol (PCP) in soil using a novel high-flow sealed test system. Pentachlorophenol has been widely used as a wood preservative, and this highly toxic biocide contaminates soil and ground water at many sites. Although plants are known to accelerate the rates of degradation of certain soil contaminants, this approach has not been thoroughly investigated for PCP. The fate of [14C]PCP, added to soil at a concentration of 100 mg/kg, was compared in three unplanted and three planted systems. The plant used was Hycrest, a perennial, drought-tolerant cultivar of crested wheatgrass [Agropyron desertorum (Fischer ex Link) Schultes]. The flow-through test system allowed us to maintain a budget for 14C-label as well as monitor mineralization (breakdown to 14CO2) and volatilization of the test compound in a 155-d trial. In the unplanted systems, an average of 88% of the total radiolabel remained in the soil and leachate and only 6% was mineralized. In the planted system, 33% of the radiolabel remained in the soil plus leachate, 22% was mineralized, and 36% was associated with plant tissue (21% with the root fraction and 15% with shoots). Mineralization rates were 23.1 mg PCP mineralized kg-1 soil in 20 wk in the planted system, and for the unplanted system 6.6 mg PCP kg-1 soil for the same time period. Similar amounts of volatile organic material were generated in the two systems (1.5%). Results indicated that establishing crested wheatgrass on PCP-contaminated surface soils may accelerate the removal of the contaminant.

In situ microbial detection in Mojave Desert soil using native fluorescence.

We report on the use of a portable instrument for microbial detection in the Mojave Desert soil and the potential for its use on Mars. The instrument is based on native fluorescence and employs four excitation wavelengths combined with four emission wavelengths. A soil dilution series in which known numbers of Bacillus subtilis spores were added to soil was used to determine the sensitivity of the instrument. We found that the fluorescence of the biological and organic components of the desert soil samples studied can be as strong as the fluorescence of the mineral component of these soils. Using the calibration derived from B. subtilis spores, we estimated that microbial content at our primary sampling site was 10(7) bacteria per gram of soil, a level confirmed by phospholipid fatty acid analysis. At a nearby site, but in a slightly different geological setting, we tested the instrument's ability to map out microbial concentrations in situ. Over a ∼50 m diameter circle, soil microbial concentrations determined with the B. subtilis calibration indicate that the concentrations of microorganisms detected varies from 10(4) to 10(7) cells per gram of soil. We conclude that fluorescence is a promising method for detecting soil microbes in noncontact applications in extreme environments on Earth and may have applications on future missions to Mars.

Diversity of soil mycobacterium isolates from three sites that degrade polycyclic aromatic hydrocarbons.

AIMS: This paper investigates the diversity of polycyclic aromatic hydrocarbon (PAH)-degrading mycobacterium isolates from three different sites within United States: Montana, Texas and Indiana. METHODS AND RESULTS: All five mycobacterium isolates differed in chromosomal restriction enzyme-fragmentation patterns; three isolates possessed linear plasmids. The DNA sequence between the murA and rRNA genes were divergent but the sequence upstream of nidBA genes, encoding a dioxygenase involved in pyrene oxidation, was more highly conserved. Long-chain fatty acid analysis showed most similarity between three isolates from the same Montana site. All isolates were sensitive to rifampicin and isoniazid, used in tuberculosis treatment, and to syringopeptins, produced by plant-associated pseudomonads. Biofilm growth was least for isolate MCS that grew on plate medium as rough-edged colonies. The patterns of substrate utilization in Biolog plates showed clustering of the Montana isolates compared with Mycobacterium vanbaalenii and Mycobacterium gilvum. CONCLUSION: The five PAH-degrading mycobacterium isolates studied differ in genetic and biochemical properties. SIGNIFICANCE AND IMPACT OF THE STUDY: Different properties with respect to antibiotic susceptibility, substrate utilization and biofilm formation could influence the survival in soil of the microbe and their suitability for use in bioaugmentation.

Polycyclic aromatic hydrocarbon-degrading Mycobacterium isolates: their association with plant roots.

Five environmental mycobacterium isolates that degrade polycyclic aromatic hydrocarbons (PAHs) were associated with barley root surfaces after growth of the seedlings from inoculated seed. Mycobacterium cells were detected along the total root length for four of these isolates. These PAH-degrading mycobacterium strains had hydrophilic cell surfaces, whereas one strain, MCS, that was hydrophobic had reduced association along the root length with no cells being detected from the root tips. The root-tip-competent strain, KMS, was competitive for its root association in the presence of the root-colonizing pseudomonad, Pseudomonas putida KT2440. All mycobacterium strains utilized simple sugars (fructose, glucose) and the trisaccharide 6-kestose, present in barley root washes, for planktonic growth, but they differed in their potential for biofilm formation under in vitro conditions. Mineralization of pyrene by the KMS strain occurred when the components in the barley root wash were amended with labeled pyrene suggesting to us that mineralization could occur in plant rhizospheres containing such mycobacterium strains.

Humic acid toxicity in biologically treated soil contaminated with polycyclic aromatic hydrocarbons and pentachlorophenol.

Contaminated soil from a land treatment unit at the Libby Groundwater Superfund Site in Libby, MT, was amended with 14C pyrene and incubated for 396 days to promote biodegradation and the formation of soil-associated bound residues. Humic and fulvic acids were extracted from the treated soil microcosms and analyzed for the presence of pyrene residues. Biologic activity promoted 14C association with the fulvic acid fraction, but humic acid-associated 14C did not increase with biologic activity. The Aboatox flash toxicity assay was used to assess the toxicity of humic and fulvic acid fractions. The fulvic acid gave no toxic response, but the humic acid showed significant toxicity. The observed toxicity was likely associated with pentachlorophenol, a known contaminant of the soil that was removed by solvent extraction of the humic acid and that correlated well with toxicity reduction.

Soil remediation techniques at uncontrolled hazardous waste sites. A critical review.

The objective of this critical review is to address soil remediation techniques at uncontrolled hazardous waste sites with regard to the following areas: 1) important regulatory and technical issues and information needs concerning soil remediation at uncontrolled hazardous waste sites; 2) approaches for selection of remediation techniques; and 3) the current state of knowledge regarding soil remediation techniques, including applications and limitations. The areas identified above are addressed with regard to current information, selected milestone publications, and specific applications of technologies to provide a synthesis of the topic. The information concerning current issues, approaches, and soil remediation techniques presented was critically reviewed in order to: 1) identify deficiencies in current approaches; 2) develop a conceptual framework for remediation; and 3) recommend improved approaches for selection of remediation technologies.

Extraction of polycyclic aromatic hydrocarbons from spiked soil.

A homogenization method was evaluated for extracting polycyclic aromatic hydrocarbons (PAHs) from soils. Fifteen PAHs were spiked and recovered from 2 soils at concentrations ranging from 1 to 1000 micrograms/g, using the homogenization method and a Soxhlet extraction method. Each extraction method performed well in removing the 15 PAHs from both soils over a broad range of concentrations. In general, Soxhlet extraction yielded slightly but significantly (P less than 0.05) higher recoveries than did the homogenization method. The homogenization method, however, was easy to use, and the extraction step turnaround time was less than 15 min/sample. The method should be suitable for other applications requiring the extraction of hydrophobic organic compounds from soils.

Reovirus removal and inactivation by slow-rate sand filtration.

Laboratory column studies were conducted at the Utah Water Research Laboratory, Logan, Utah, to evaluate reovirus removal from drinking water supplies by slow-rate sand filtration (SSF). Columns, constructed to simulate a full-scale SSF field operation, were inoculated with reovirus at ca. 1,000-times-greater concentrations than those typically found in domestic sewage. Reovirus removal and inactivation were investigated as functions of filter maturity and other filter sand characteristics. Reovirus removal studies demonstrated that the SSF process is capable of reducing reovirus in influent water by a minimum of 4 log concentration units under certain conditions of water quality, flow rate, and sand bed construction. Infectious reovirus was not detected in effluent samples from any of the sand beds studied, after inoculation of the SSF columns; therefore, removal efficiencies were not affected significantly by characteristics, including age, of the two filter sands evaluated. Studies conducted with radioactively labeled reovirus demonstrated that reovirus removed from influent water was distributed throughout the entire length of the filter beds. Concentrations of reovirus in the filter sands decreased with increasing bed depth. The greatest removal occurred in the top few centimeters of all sand beds. No infectious reovirus could be detected in clean or mature sand bed media, indicating that reoviruses were inactivated in the filter.

Isolation and characterization of polycyclic aromatic hydrocarbon-degrading Mycobacterium isolates from soil.

Bioremediation of soils contaminated with wood preservatives containing polycyclic aromatic hydrocarbons (PAHs) is desired because of their toxic, mutagenic, and carcinogenic properties. Creosote wood preservative-contaminated soils at the Champion International Superfund Site in Libby, Montana currently undergo bioremediation in a prepared-bed land treatment unit (LTU) process. Microbes isolated from these LTU soils rapidly mineralized the (14)C-labeled PAH pyrene in the LTU soil. Gram staining, electron microscopy, and 16S rDNA-sequencing revealed that three of these bacteria, JLS, KMS, and MCS, were Mycobacterium strains. The phylogeny of the 16S rDNA showed that they were distinct from other Mycobacterium isolates with PAH-degrading activities. Catalase and superoxide dismutase (SOD) isozyme profiles confirmed that each isolate was distinct from each other and from the PAH-degrading mycobacterium, Mycobacterium vanbaalenii sp. nov, isolated from a petroleum-contaminated soil. We find that dioxygenase genes nidA and nidB are present in each of the Libby Mycobacterium isolates and are adjacent to each other in the sequence nidB-nidA, an order that is unique to the PAH-degrading mycobacteria.