RESUMEN
The molecular characterization of post-receptor actors involved in insect olfactory transduction has yet to be understood. We have investigated the presence of a Transient Receptor Potential (TRP) channel in the peripheral olfactory system of the moth Spodoptera littoralis. A cDNA encoding a Lepidopteran TRP channel (TRPgamma) was identified by analysis of a male-antennal EST database and subsequently cloned by RACE PCR. In adult males, the TRPgamma transcript was detected in antennae, at the base of olfactory sensilla. Moreover, TRPgamma was observed in antennae in both pupal and adult stages. This work is the first step in understanding the involvement of TRPgamma in signalling pathways involved in the development and function of the insect olfactory system.
Asunto(s)
Estructuras Animales/metabolismo , Vías Olfatorias/metabolismo , Transducción de Señal , Spodoptera/metabolismo , Canales de Potencial de Receptor Transitorio/metabolismo , Secuencia de Aminoácidos , Animales , Clonación Molecular , Perfilación de la Expresión Génica , Regulación del Desarrollo de la Expresión Génica , Masculino , Datos de Secuencia Molecular , Filogenia , Análisis de Secuencia de ADN , Canales de Potencial de Receptor Transitorio/química , Canales de Potencial de Receptor Transitorio/genéticaRESUMEN
In the aim of the characterization of the molecular actors of insect olfactory transduction, we have cloned the full cDNA encoding a Spodoptera littoralis diacylglycerol kinase (DGK) named SlDGK. In male adults, SlDGK transcript was detected predominantly in the brain and in the olfactory sensilla trichodea located on the antennae. SlDGK expression was first detected at day 3 of the pupal stage, then reached a maximum at the end of this stage and was maintained at this level during the adult period. These data provide the first molecular characterization of a DGK potentially involved in the regulation of signalling pathways responsible for the establishment and/or the functioning of the olfactory system in Lepidoptera.