RESUMEN
Some cytological and morphological characteristics of haploid and dihaploid plants of winter rape obtained via anther culture were studied. It was shown that in haploid plants the number of chloroplasts in stomata guard cells and the size of the stomata guard cells themselves were much smaller, and the number of stomata per unit area was greater than in doubled haploids and diploids. Haploids were also characterized by a smaller size of petals and anthers, and in general, a smaller flower compared to dihaploids and diploids.
Asunto(s)
Brassica napus/citología , Brassica napus/genética , Cromosomas de las Plantas/genética , Diploidia , Haploidia , Brassica napus/crecimiento & desarrollo , Cloroplastos/genética , Cloroplastos/ultraestructura , Flores/citología , Flores/genética , Flores/crecimiento & desarrollo , Polen/citología , Polen/genética , Polen/crecimiento & desarrolloRESUMEN
Regeneration ability in callus cultures from anthers of two hybrid genotypes of oil flax was studied on N6 and LMA-1 nutrient media at various concentrations of cytokinine 6-benzylamynopurine (BAP). It was shown that callus grew and developed better at BAP concentrations of 2 mg/l, comparing with 4 and 6 mg/l. Shoot and root regeneration was observed in F1 genotype 6-8-gnezdny x M22 only and did not depend on BAP concentration in the medium and on the medium composition itself. Transfer onto fresh medium often stimulated dedifferentiation of the regenerated structures.
Asunto(s)
Adenina/análogos & derivados , Medios de Cultivo/química , Medios de Cultivo/farmacología , Lino/fisiología , Regeneración , Adenina/farmacología , Compuestos de Bencilo , Técnicas de Cultivo , Lino/embriología , Cinetina , Reguladores del Crecimiento de las Plantas/farmacología , PurinasRESUMEN
Influence of selection of F1 high temperature tolerant pollen on the structure of BC1 segregating populations was studied for a number of agronomically important traits in oil flax. It was found that heat treatment of heterogeneous pollen population considerably changed genetic composition of plant population not only for tolerance to drought but for duration of seedling emergence--flowering period and quantity and inclination angle of side shoots as well. It is supposed that genes which confer these traits are at least partially linked to the genes responsible for pollen sensitivity to high temperature.