RESUMEN
A latex particle immunoassay has been developed for the quantification of choriogonadotropin in human serum using two monoclonal antibodies specific for the beta-chain of the hormone. The assay, based on optical counting of monomeric particles, was achieved in 40 min and the calibration curve was linear between 10 and 200 IU/l. Intra- and interassay precisions at three different levels of the curve varied between 3.3 and 10.9%. The method was validated by comparison with two different radioimmunoassays and correlation coefficients of 0.97-0.99 were obtained.
Asunto(s)
Gonadotropina Coriónica/sangre , Anticuerpos Monoclonales , Especificidad de Anticuerpos , Calibración , Humanos , Inmunoensayo/métodos , Pruebas de Fijación de Látex/métodos , Pepsina A , Reproducibilidad de los ResultadosRESUMEN
We describe here two latex immunoassays for total thyroxine (T4) and total triiodothyronine (T3). These homogeneous 60 min assays are quantified by optically counting the monomeric particles remaining after agglutination. When precision is assessed, both methods display coefficients of variation of 3-7% for within-run assays and 4-10% for between-run assays. The accuracy of the methods, as tested by dilution and spike recovery experiments, was found to be satisfactory. Two correlation studies were carried out to compare the present method with leading commercial methods. The coefficients obtained were: r = 0.92 and r = 0.93 with 150 sera for T3, and r = 0.95 (150 sera) and r = 0.93 (108 sera) for T4.
Asunto(s)
Tiroxina/análisis , Triyodotironina/análisis , Relación Dosis-Respuesta Inmunológica , Humanos , Inmunoensayo/métodos , Pruebas de Fijación de LátexRESUMEN
During an immune response, the increase in binding affinity of antibodies is followed by a fall. Lymphocytes bearing autoanti-idiotypic receptors were detected during a normal immune response. The kinetics of appearance and disappearance of such lymphocytes led us to propose a network model to explain the changes occurring in antibody properties during an immune response.
Asunto(s)
Anticuerpos Antivirales/biosíntesis , Afinidad de Anticuerpos , Sitios de Unión de Anticuerpos , Linfocitos/inmunología , Animales , Especificidad de Anticuerpos , Autoanticuerpos , Cinética , Conejos , Linfocitos T/inmunología , Virus del Mosaico del Tabaco/inmunologíaRESUMEN
Pregnancy-specific beta 1-glycoprotein (SP1) was assayed by particle-counting immunoassay in serum from 86 healthy blood donors and 236 patients with various types of gammopathy. A concentration of 1 microgram/L was taken as the upper normal limit. Abnormally high values were found in one of 10 patients with monoclonal gammopathy of undetermined significance, in 65% of 152 patients with multiple myeloma, in 84% of 64 patients with Waldenström's macroglobulinemia, and in seven of 10 patients with monoclonal gammopathies associated with other myeloproliferative disorders. In a study of 90 myeloma patients, the SP1 value correlated (p less than 0.001) with the concentration of beta 2-microglobulin in serum, a value which had been corrected for possible renal dysfunction, but not with the concentration of the monoclonal component. SP1 was detected by direct immunofluorescence in myeloma cells of bone-marrow smears from six of 10 patients with myelomatosis. These six patients had serum SP1 values greater than 1 microgram/L, whereas the four patients with fluorescence-negative myeloma cells had SP1 values less than 1 microgram/L.
Asunto(s)
Paraproteinemias/sangre , Proteínas Gestacionales/análisis , Glicoproteínas beta 1 Específicas del Embarazo/análisis , Microglobulina beta-2/análisis , Adulto , Anciano , Electroforesis en Gel de Agar , Femenino , Técnica del Anticuerpo Fluorescente , Humanos , Inmunoensayo/métodos , Inmunoglobulina A/análisis , Inmunoglobulina G/análisis , Inmunoglobulina M/análisis , Masculino , Persona de Mediana Edad , Mieloma Múltiple/sangre , Macroglobulinemia de Waldenström/sangreRESUMEN
Circulating concentrations of leucocyte elastase were measured in 16 adult patients undergoing cardiopulmonary bypass (CPB) with a flat-sheet membrane oxygenator. Eight patients (Group I) received the calcium channel blocker nifedipine (9 micrograms.kg-1 x h-1) during CPB. Eight patients (Group II) did not receive any calcium channel blocker during surgery and served as the control group. Elastase concentrations were measured at 7 time points: 2 before, 2 during, and 3 after CPB. The bypass procedure was associated with elevation in elastase concentrations (P < 0.001). Comparing to baseline values elastase concentrations were significantly elevated (P < 0.05) 60 min after the start of CPB and on all measurements done after CPB. Elastase concentrations correlated with the duration of CPB (rs = 0.76, P < 0.001), and were not influenced by nifedipine infusion as revealed by comparing the two groups. This study demonstrates moderate elastase release during CPB with a flat-sheet membrane oxygenator and fails to confirm inhibition of elastase release by nifedipine infusion during CPB.
Asunto(s)
Puente Cardiopulmonar , Leucocitos/enzimología , Nifedipino/farmacología , Elastasa Pancreática/sangre , Anciano , Femenino , Humanos , Masculino , Persona de Mediana EdadRESUMEN
Irradiated rabbits grafted with allogeneic lymph node, spleen and bone marrow cells from a donor rabbit hyperimmunized against TMV synthesize high affinity antibodies, displaying mainly recipient allotypic specificities, after antigen boosting. By contrast, recipient rabbits from non-immune donors synthesize antibodies of lower affinity. It is suggested that the differentiation of new emerging host B cells is specifically influenced by the presence of donor-memory cells.
Asunto(s)
Anticuerpos Antivirales/biosíntesis , Afinidad de Anticuerpos , Sueros Inmunes , Inmunización Pasiva , Absorción , Animales , Inmunodifusión , Alotipos de Inmunoglobulinas , Transfusión de Linfocitos , Conejos , Bazo/inmunología , Virus del Mosaico del Tabaco/inmunología , Trasplante Homólogo , Rayos XRESUMEN
Rabbits hyperimmunized with tobacco mosaic virus synthesize very heterogeneous antibodies. Despite this, specific anti-idiotypic sera recognizing a large part (70%) of these antibodies can be raised in rabbits matched for allotypic specificities a1, a2, a3, b4, b5, b6, c7, and b9. Different rabbits synthesize antibodies with different idiotypic specificities. However, in the serum of a single rabbit antibody fractions of different isoelectric pH share some idiotypic specificities. The results show that, at least in certain cases, antibodies against one antigen are not simply a random collection of immunoglobulin which happen to fit with this antigen, but that some definite relationship exists between the products of different clones which have been activated by antigen. These findings are discussed in the light of network concepts of the immune system.
Asunto(s)
Anticuerpos Antivirales/análisis , Virus del Mosaico del Tabaco/inmunología , Animales , Especificidad de Anticuerpos , Células Clonales/inmunología , Epítopos , Alotipos de Inmunoglobulinas , Fragmentos Fab de Inmunoglobulinas/análisis , Cadenas kappa de Inmunoglobulina , Focalización Isoeléctrica , ConejosRESUMEN
Irradiated rabbits were grafted with a mixture of bone marrow, lymph node and spleen cells from donors hyperimmunized against TMV. Recipient and donors were characterized by different a allotypic specificities. Antibodies synthesized in the recipients display allotypic markers from the recipients but idiotypic specificities cross-reactive with those of donor antibodies. The results show that the differentiation of new host B cells is influenced by the presence of donor memory cells and are interpreted in the light of network concepts.
Asunto(s)
Anticuerpos Antivirales/biosíntesis , Alotipos de Inmunoglobulinas , Idiotipos de Inmunoglobulinas , Inmunoglobulinas/biosíntesis , Transfusión de Linfocitos , Animales , Sitios de Unión de Anticuerpos , Reacciones Cruzadas , Cobayas , Sueros Inmunes , Conejos , Bazo/inmunología , Virus del Mosaico del Tabaco/inmunología , Trasplante Homólogo , Rayos XRESUMEN
Common variable immunodeficiency (CVI) patients require regular intravenous immunoglobulin substitution therapy (IVGG). We studied eight patients; four of whom had adverse reactions to IVGG, and found that those coincided with elevated tumor necrosis factor-alpha (TNF-alpha) levels during infusion. Those reactions and TNF production were abolished by switching from one IVGG preparation to another in two patients. Reappearance of adverse reactions after switching preparation was preceded by a progressive rise in peak TNF levels in one patient.
Asunto(s)
Inmunodeficiencia Variable Común/tratamiento farmacológico , Inmunodeficiencia Variable Común/metabolismo , Inmunoglobulinas Intravenosas/efectos adversos , Inmunoglobulinas Intravenosas/uso terapéutico , Factor de Necrosis Tumoral alfa/metabolismo , Adolescente , Adulto , Anciano , Anciano de 80 o más Años , Aspirina/uso terapéutico , Niño , Relación Dosis-Respuesta a Droga , Femenino , Humanos , MasculinoRESUMEN
Intracytoplasmic lamellar organelles identical in ultrastructure to surfactant-containing lamellar bodies found in type II pneumocytes, have been demonstrated in other tissues, in synoviocytes and mesothelial cells, in a distribution pattern which reflects the systemic expression of rheumatoid disease. Antibodies raised against surfactant protein A (SP-A), exhibit a ranking of tissue reactivity in area, intensity and density of cells which also parallels the frequency and degree of pathological involvement characteristic of rheumatoid disease, showing in ascending order of immunopositivity, lacrymal and salivary epithelia, pulmonary parenchyma, mesothelium and synoviocytes. Maximal tissue reactivity to anti-SP-A antibodies was found in the synovium of 55 rheumatoid patients exhibiting classical histopathological appearances of RA, in a pattern of immunostaining identical to that obtained with ML30, an antibody to mycobacterial heat shock protein 65kDa which, in turn, cross-reacted with SP-A in dot blot testing.
Asunto(s)
Artritis Reumatoide/patología , Orgánulos/ultraestructura , Proteolípidos/análisis , Surfactantes Pulmonares/análisis , Membrana Serosa/ultraestructura , Membrana Sinovial/ultraestructura , Epitelio/química , Epitelio/ultraestructura , Humanos , Microscopía Inmunoelectrónica , Proteína A Asociada a Surfactante Pulmonar , Proteínas Asociadas a Surfactante Pulmonar , Membrana Serosa/química , Membrana Sinovial/químicaRESUMEN
The intra- and extracellular distribution and relative density of lamellar bodies (LBs) were determined by electron microscopy in synovial biopsies from 20 non-rheumatoid arthritis (RA) patients. LBs were found on the synovial surface, in intimal cells, throughout intimal matrix, in blood vessel walls, in endothelial cytoplasm and within vascular lumena. Lamellar profiles were observed in type B synoviocytes within rough endoplasmic reticulum (RER), in association with the Golgi apparatus, and embedded in electron dense matrix (projection cores) in multivesicular bodies. Exocytotic release of mature LBs into intimal matrix was observed. In type A synoviocytes the outer lamellae of LBs were frequently found in contiguity with the limiting membrane of lysosomes. An in vitro investigation of the ultrastructural features of LB formation in cultured type B synoviocytes (from 3 non-RA patients) gave results similar to those obtained in biopsies. These studies provide ultrastructural evidence of synoviocyte activity in secreting and degrading phospholipid lubricant in a sophisticated system whose function and pathological derangements are largely unknown.
Asunto(s)
Orgánulos/ultraestructura , Membrana Sinovial/ultraestructura , Humanos , Microscopía Electrónica , Orgánulos/metabolismo , Membrana Sinovial/citologíaRESUMEN
In vitro incubation of human cytomegalovirus (Towne strain) with 8 U/ml human recombinant myeloperoxidase plus sodium chloride and glucose nearly abolished viral infectivity. To assay the effect on intracellular infection, cell toxicity of the enzymes was first studied. Even the high dose of 16 U/ml of recombinant myeloperoxidase plus 10 mU/ml glucose oxidase did not decrease MRC5 cell growth. By contrast, this dose reduced proliferation of activated THP1 cells. Even half of the myeloperoxidase dose proved slightly toxic to these cells. Non-cytotoxic concentrations of the reagents were used to monitor their effect on cytomegalovirus infection. In MRC5 cells, even the low dose of 4 U/ml myeloperoxidase plus glucose oxidase inhibited synthesis of cytomegalovirus early antigens, as tested by immunofluorescence. Viral release in the supernatant was decreased by 4 logs. In THP1 cells, which produce endogenously hydrogen peroxide, myeloperoxidase alone (8 U/ml) decreased the formation of early and late antigens by 53 and 44%, respectively.
Asunto(s)
Antivirales/farmacología , Infecciones por Citomegalovirus/virología , Citomegalovirus/efectos de los fármacos , Peroxidasa/farmacología , Proteínas Recombinantes/farmacología , División Celular , Línea Celular , Citomegalovirus/patogenicidad , Fibroblastos/fisiología , Fibroblastos/virología , Humanos , Peróxido de Hidrógeno/metabolismo , Macrófagos/fisiología , Macrófagos/virología , Monocitos/fisiología , Monocitos/virología , Peroxidasa/genética , Proteínas Recombinantes/genética , Replicación Viral/efectos de los fármacosRESUMEN
Characterization of the spontaneous immune response that frequently occurs in tumor-bearing animals, as well as immunization using dendritic cells pulsed with tumor antigens, suggests that a limiting factor of the tumor-specific immune response may be a defect in the co-stimulatory signal that is required for optimal activation of T cells. In this work, we describe a new approach to improve the antigen-presenting capacity of tumor cells, which does not require a source of purified tumor-associated antigen. We fused P815 mastocytoma cells with bone marrow-derived dendritic cells. We obtained one hybrid that displayed the phenotypic and functional properties of dendritic cells and expressed mRNA coding for the tumor-associated antigen P815 A/B. Injections of irradiated hybrid cells prevented the growth of preimplanted mastocytoma and induced long-lasting tumor resistance.