RESUMEN
Controversial results regarding the presence and role of human papillomavirus in the development of oesophageal squamous cell carcinoma have been published. We used multiple broad-spectrum polymerase chain reactions to identify HPV DNA in oesophageal carcinomas from a low-incidence area. Paraffin embedded- and snap-frozen specimens from oesophageal cancer tissues of 63 patients were examined with a PCR technique with several primer pairs, capable of detecting most known HPV types. In none of the oesophagus cancer tissues could HPV DNA be detected. The role of HPV in this type of carcinoma in a low incidence area remains unclear.
Asunto(s)
Carcinoma de Células Grandes/virología , Carcinoma de Células Escamosas/virología , Neoplasias Esofágicas/virología , Infecciones Tumorales por Virus/complicaciones , Adulto , Anciano , Carcinoma de Células Grandes/epidemiología , Carcinoma de Células Escamosas/epidemiología , ADN Viral/aislamiento & purificación , Neoplasias Esofágicas/epidemiología , Femenino , Humanos , Incidencia , Masculino , Persona de Mediana Edad , Países Bajos/epidemiología , Papillomaviridae/aislamiento & purificación , Reacción en Cadena de la PolimerasaRESUMEN
The localization of human papillomavirus type 16 (HPV-16) DNA throughout the cervix uteri of women with cervical intraepithelial neoplasia (CIN) was studied by utilizing the polymerase chain reaction technique directly on histologically defined sections of paraffin-embedded cervical tissue obtained by conizations. HPV-16 DNA was detected only in the sections that contained CIN lesions and/or koilocytes. No HPV-16 DNA was detected in sections that contained only normal epithelium. This is in accordance with HPV-16 playing a role in the development of CIN lesions.