RESUMEN
BACKGROUND: It is important to identify the behavior of colorectal cancer (CRC) individually, so more accurate laboratory index is urgently demanded. Chaperonin are key molecules in tumor cell cycle. Our study aims at revealing the expression and correlation of chaperonin containing TCP1 complex 6A (CCTζ-1) in CRC. METHODS: Fifty-eight patients with CRC admitted from 2005 to 2008 were selected. CCTζ-1 expression in cell, tumor, and non-tumor colorectal tissues was detected by Western blot, and their protein was localized by immunohistochemical stain. After HCT116 cells were transfected with CCTζ-1 siRNA, real-time PCR, and Western blot were used to examine gene expression. Cell multiplication and apoptosis were examined by Cell Counting Kit-8 and Annexin V kit. RESULTS: CCTζ-1 ptotein expression was detected in 51 of 58 (87.9%) CRC specimens, which was much higher than those in normal mucosa (P < 0.01), and it was correlated with tumor invasion (P < 0.01) and tumor size (P < 0.05). The levels of CCTζ-1 mRNA and protein were inhibited by CCTζ-1 siRNA in HCT116 cells transfected with CCTζ-1 siRNA, which resulted in growth arrest but not apoptosis. CONCLUSION: CCTζ-1 could be a new prognostic marker for CRC and involve in tumorigenesis. CCTζ-1 inhibition in vivo might therefore become a new therapy for CRC.
Asunto(s)
Proliferación Celular , Chaperonina con TCP-1/metabolismo , Neoplasias Colorrectales/metabolismo , Regulación Neoplásica de la Expresión Génica , Proteínas de Neoplasias/metabolismo , Anciano , Chaperonina con TCP-1/antagonistas & inhibidores , Chaperonina con TCP-1/genética , Neoplasias Colorrectales/genética , Neoplasias Colorrectales/patología , Femenino , Células HCT116 , Humanos , Técnicas In Vitro , Masculino , Persona de Mediana Edad , Proteínas de Neoplasias/antagonistas & inhibidores , Proteínas de Neoplasias/genética , ARN Mensajero/metabolismo , ARN Interferente PequeñoRESUMEN
Prof. XIE Xi-liang in his early years was a disciple of Mr. CHENG Dan-an. He has been engaged in clinical work and teaching for over 60 years with rich experience, and he pays attention to application of acupuncture, moxibustion and medicine for different persons, especially pays attention to moxibustion methods, such as direct moxibustion, with less points selected, and good results, convenient manipulation, moxibustion of one session only for 3-5 min. He teaches the patients and their family for self-moxibustion at home, saving time. This method has better short-term and long-term therapeutic effects for hypo-immune patients, pain diseases, and it is suitable to treatment of diseases and health-care.