RESUMEN
During the last decade the number of investigations on the preparation and application of more effective drug release systems on the basis of nanocarriers from biocompatible and biodegradable polymers are considerably increasing. This is notably in force for practically water insoluble drugs to be applied in liquid forms (eye solutions for an example). The aim of the work presented was the preparation of model poly(vinyl acetate) and poly(styrene) nanosupports for indometacin and their potential inclusion in eye drops. The polymers are synthesized as nanosized latex by a radical polymerization of the monomers in the presence of indometacin. It is proved that the low polymerization temperature and initiator used do not influence indometacin structure and properties. The nanoparticles were characterized by attenuated total reflection Fourier transform infrared spectroscopic analyses, atomic force microscopy, scanning electron microscopy and transmission electron microscopy. The size of the latex particles was around 200 nm, determined by the scan electron microscopy. The indometacin delivery rate from the supports discussed in aqueous solutions was determined at pH 7.4. The change of this rate, in comparison with that for a pure drug substance, was established also as well as its dependence on the nature of the carrier.
Asunto(s)
Antiinflamatorios no Esteroideos/química , Indometacina/química , Microesferas , Nanopartículas/química , Poliestirenos/química , Polivinilos/química , Antiinflamatorios no Esteroideos/administración & dosificación , Portadores de Fármacos , Indicadores y Reactivos , Indometacina/administración & dosificación , Cinética , Microscopía de Fuerza Atómica , Microscopía Electrónica de Rastreo , Microscopía Electrónica de Transmisión , Soluciones Oftálmicas , Tamaño de la Partícula , Solubilidad , Espectrofotometría Ultravioleta , Espectroscopía Infrarroja por Transformada de FourierRESUMEN
Complexes formed between DNA and cationic polymers are attracting increasing attention as novel synthetic vectors for delivery of genes. We are trying to improve biological properties of such complexes by oriented self-assembly of DNA with cationic-hydrophilic block copolymers, designed to enshroud the complex within a protective hydrophilic polymer corona. Poly(L-lysine) (pLL) grafted with range of hydrophilic polymer blocks, including poly(ethylene glycol) (pEG), dextran and poly[N-(2-hydroxypropyl)methacrylamide] (pHPMA), shows efficient binding to DNA and mediates particle self-assembly and inhibition of ethidium bromide/DNA fluorescence. The complexes formed are discrete and typically about 100 nm diameter, viewed by atomic force microscopy. Surface charges are slightly shielded by the presence of the hydrophilic polymer, and complexes generally show decreased cytotoxicity compared with simple pLL/DNA complexes. pEG-containing complexes show increased transfection activity against cells in vitro. Complexes formed with all polymer conjugates showed greater aqueous solubility than simple pLL/DNA complexes, particularly at charge neutrality. These materials appear to have the ability to regulate the physicochemical and biological properties of polycation/DNA complexes, and should find important applications in packaging of nucleic acids for specific biological applications.
Asunto(s)
ADN/metabolismo , Marcación de Gen/métodos , Genes Sintéticos , Vectores Genéticos/síntesis química , Vectores Genéticos/metabolismo , Polilisina/genética , Polilisina/metabolismo , Secuencia de Aminoácidos , Animales , Biopolímeros/metabolismo , Biopolímeros/toxicidad , Bovinos , Línea Celular Transformada , Electroforesis en Gel de Agar , Feto , Vectores Genéticos/toxicidad , Humanos , Riñón , Neoplasias Hepáticas , Microscopía de Fuerza Atómica , Datos de Secuencia Molecular , Peso Molecular , Tamaño de la Partícula , Polilisina/análogos & derivados , Solubilidad , Espectrometría de Fluorescencia , Propiedades de Superficie , Transfección/efectos de los fármacos , Células Tumorales Cultivadas , Agua/metabolismoRESUMEN
Cationic polymers can self-assemble with DNA to form polyelectrolyte complexes capable of gene delivery, although biocompatibility of the complexes is generally limited. Here we have used A-B type cationic-hydrophilic block co-polymers to introduce a protective surface hydrophilic shielding following oriented self-assembly with DNA. Block co-polymers of poly(ethylene glycol)-poly-L-lysine (pEG-pLL) and poly-N-(2-hydroxypropyl)methacrylamide-poly(trimethylammonioethyl methacrylate chloride) (pHPMA-pTMAEM) both show spontaneous formation of complexes with DNA. Surface charge measured by zeta potential is decreased compared with equivalent polycation-DNA complexes in each case. Atomic force microscopy shows that pHPMA-pTMAEM/DNA complexes are discrete spheres similar to those formed between DNA and simple polycations, whereas pEG-pLL/DNA complexes adopt an extended structure. Biological properties depend on the charge ratio of formation. At optimal charge ratio, pEG-pLL/DNA complexes show efficient transfection of 293 cells in vitro, while pHPMA-pTMAEM/DNA complexes are more inert. Both block co-polymer-DNA complexes show only limited cytotoxicity. Careful selection of block co-polymer structure can influence the physicochemical and biological properties of the complexes and should permit design of materials for specific applications, including targeted delivery of genes in vivo.
Asunto(s)
ADN/química , Terapia Genética/métodos , Vectores Genéticos/química , Vectores Genéticos/ultraestructura , Conformación de Ácido Nucleico , Polímeros/síntesis química , Supervivencia Celular , Células Cultivadas , ADN/ultraestructura , Electroforesis en Gel de Agar , Galactosidasas/genética , Regulación de la Expresión Génica , Humanos , Microscopía de Fuerza Atómica , Estructura Molecular , Propiedades de Superficie , TransfecciónRESUMEN
Poly(ethylene glycol)-dextran (PEG-DEX) conjugates have been used as a combined stabilizer and surface modifier to produce resorbable poly(DL-lactide-co-glycolide) (PLG) microparticles by an emulsification/solvent evaporation technique. The use of PEG or dextran polymers alone was incapable of producing microparticles. Particle size measurements revealed smaller mean particle sizes (480 nm) and improved polydispersity when using a 1.2% PEG substituted conjugate relative to a 9% substituted material (680 nm). PLG microparticles modified by post-adsorbed PEG-DEX conjugates flocculated in 0.01 M salt solutions, whereas PLG microparticles prepared using PEG-DEX as a surfactant were stable in at least 0.5 M NaCl solutions. Surface modification of PLG microparticles was confirmed by zeta potential measurements and surface analysis using X-ray photoelectron spectroscopy. The presence of surface exposed dextran was confirmed by an immunological detection method using a dextran-specific antiserum in an enzyme-linked immunosorbent assay. The findings support a model in which the PEG component of the PEG-DEX conjugate provides an anchor to the microparticle surface while the dextran component extends from the particle surface to contribute a steric stabilization function. This approach offers opportunities for attaching hydrophilic species such as targeting moieties to biodegradable microparticles to improve the interaction of drug carriers and vaccines with specific tissue sites.
Asunto(s)
Materiales Biocompatibles/metabolismo , Dextranos/metabolismo , Sistemas de Liberación de Medicamentos , Ácido Láctico/metabolismo , Polietilenglicoles/metabolismo , Ácido Poliglicólico/metabolismo , Polímeros/metabolismo , Vacunas/administración & dosificación , Materiales Biocompatibles/química , Dextranos/química , Portadores de Fármacos , Estabilidad de Medicamentos , Emulsiones , Ensayo de Inmunoadsorción Enzimática , Microscopía Electrónica de Rastreo , Microesferas , Tamaño de la Partícula , Polietilenglicoles/química , Copolímero de Ácido Poliláctico-Ácido Poliglicólico , Solventes , Espectrometría por Rayos X , Propiedades de Superficie , VolatilizaciónRESUMEN
This paper describes the synthesis and evaluation of biodegradable derivatives of poly-L-glutamic acid as suitable vectors for gene therapy. When mixed with DNA the new polymers self assemble and form polyelectrolyte complexes. The formation of the complexes and determination of their stability towards disruption by serum albumin was monitored by Ethidium bromide (EtBr) fluorescence spectroscopy. All polymers were able to form complexes and their size, determined by photon correlation spectroscopy, was between 84.5+/-2 nm and 96. 7+/-1.6 nm, depending on the type of polymer and the charge ratio. All complexes were stable towards serum albumin. The results from the biodegradability tests, using tritosomes, show that the polymers are biodegradable and the rate of degradation is influenced by the number of charged groups in the side chains. Haemolysis and red blood cell (RBC) agglutination were assessed and compared to poly(L-lysine) (pLL) and polyethyleneimine (pEI). RBC agglutination was monitored with optical microscopy. Results show that the new polymers are less toxic than pLL and pEI. Preliminary transfection studies show that the polymers are suitable vectors for gene delivery.
Asunto(s)
Terapia Genética/métodos , Vectores Genéticos/química , Ácido Poliglutámico/química , Absorciometría de Fotón , Aglutinación/efectos de los fármacos , Aminas/química , Fenómenos Químicos , Química Física , Eritrocitos/efectos de los fármacos , Etidio/química , Colorantes Fluorescentes/química , Expresión Génica , Hemólisis/efectos de los fármacos , Humanos , Técnicas In Vitro , Ensayo de Materiales , Tamaño de la Partícula , Polímeros/síntesis química , Piridinas/química , TransfecciónRESUMEN
Amphiphilic AB and ABA block copolymers have been prepared from poly (ortho esters) and poly (ethylene glycol). Such block copolymers readily form micellar dispersions in water, or buffers. The CMC is in the range of 3 x 10(-4)-5 x 10(-4) g/l which is a value low enough to assure retention of micelle integrity upon intravenous injection. The size, as determined by dynamic light scattering was in the 40-70 nm range. The micelles can be stored in lyophilized form for at lest 8 months and easily reconstituted to the original properties. The micelles are stable in PBS at pH 7.4 and 37 degrees C for 3 days and in a citrate buffer at pH 5.5 and 37 degrees C for 2 h. Stability in the presence of bovine serum albumin depends on the structure of the block copolymer and especially the length of the POE block.
Asunto(s)
Neoplasias/metabolismo , Poliésteres/química , Polietilenglicoles/química , Portadores de Fármacos , Estabilidad de Medicamentos , Concentración de Iones de Hidrógeno , Espectroscopía de Resonancia Magnética , Micelas , Peso Molecular , Neoplasias/tratamiento farmacológico , Tamaño de la Partícula , Albúmina Sérica Bovina/químicaRESUMEN
The aim of this study was to evaluate the use of cationic-hydrophilic copolymers for self-assembly with antisense oligonucleotides targeted to the bcl-2 mRNA in order to improve their biocompatibility and modulation of their pharmacokinetics for greater therapeutic usefulness. Examination of the ability of poly(trimethylammonioethyl methacrylate chloride)-poly[N-(2-hydroxypropyl)methacrylamide] (pHPMA-b-pTMAEM) block copolymers to condense the oligonucleotide by fluorescence and electrophoresis techniques showed that complexes were formed more efficiently than with copolymers containing poly(ethylene glycol) blocks grafted onto the backbone of poly(L-lysine) (pLL-g-pEG). In addition, the copolymer pTMAEM-b-pHPMA produced oligonucleotide complexes with the most favourable physicochemical properties appropriate for in vivo applications. The complexes were small (approximately 36 nm in diameter), with low surface charge as measured by zeta potential, relatively stable to physiological salt conditions and could be formed at a DNA concentration of 500 microg/ml. Complex formation with the copolymer pTMAEM-b-pHPMA or pLL-g-pEG reduced the urinary clearance of the oligonucleotide after intravenous injection into mice. However after 30 min, the oligonucleotide complexes were cleared from the bloodstream. These results indicate that for the systemic delivery of oligonucleotides the polymer-derived complexes are not stable enough for prolonged circulation. Instead, these complexes may be more suitable for localised in vivo applications.