Molecular epidemiological and serological studies of hantavirus infection in northern Vietnam.

The distribution of anti-hantavirus antibodies in humans and rodents in northern Vietnam was examined. In total, 837 serum samples from healthy humans (617) and patients with fever (220), living in six different areas were screened for IgG antibodies against Hantaan or Seoul virus (SEOV) by ELISA, IFA, and Western blot analysis. Antibody-positive sera were identified in 7/617 (1.1%) healthy donors, 5/150 port workers in the port of Hai Phong, and 2/185 residents of Ha Nam Province. In comparison, positive sera were detected in 5/220 (2.3%) fever patients in the provinces of Ha Nam (1/58) and Thanh Hoa (4/146). Antibody-positive Rattus norvegicus were found in the provinces of Ha Nam (7/52) and Thanh Hoa (1/67), in Haibatrung District (7/43) in Hanoi, and in Hai Phong Port (21/62), while antibody-positive R. rattus (2/17) were found in Hai Phong Port. Part of the Gc region from the viral genome was amplified by RT-PCR using lung tissue samples from R. norvegicus in Haibatrung (2/7) and Hai Phong Port (7/9), but not from R. rattus (0/2). Viral sequences were located in the SEOV clade and formed a single lineage with Indonesian SEOV, suggesting that Vietnamese SEOV is part of a distinct lineage among Asian SEOVs.

Preliminary study on serology of hantaviruses in rats at Lao Cai and Hoa Binh

By ELISA assay, 26 sample of rats (Lao Cai: 14 samples, Hoa Binh: 12 samples) were studies in the year 2002. Serum-positive results in suspective rats of Lao Cai accounted for 28.57%, Hoa Binh 25%. The rate of antihantanvirus antibody was 26.92% in both provinces, Positive serum sample in ELISA technique were verified with mediate immunofluorescein technique giving 14.2% positive in Lao Cai samples. The results were consistent with the epidemiological characteristics of Hantan fever, an endemic of mountain regions

Detection of a hantavirus infected patient in Hai Duong province, in 2004

A study was conducted on 18 patients with Dengue hemorrhagic fever like symptoms in July 2004 at Hai Duong province. All 18 serum samples of these patients were negative to Dengue serology tests. ELISA tests had been used after that and shown the presence of antibodies (IgG) against Hantaviruses in one of 18 patients. Moreover, anti-hantavirus antibodies un this patient was also detected by using immunochromatographic test of Standard Diagnostics Korean company

Serological investigation on hantavirus in rodent of Hanam province in 2002

42 samples of serum of Rattus novergicus in Ha Nam province were tested for Hantaan virus by ELISA and IFA methods. Results: 4/42 samples (9.25%) had anti-bodies against Hantaan virus. With IFA method, 2/4 samples were confirmed again. It is necessary to supervise serum of people in community and unknown fever

Surveillance of Hantaan virus serology in Rattus Novergicus rodent in Thanh Hoa province, 2003

Hantaan virus is the pathogene of 2 forms of high mortality of hemorrhagic fever with kidney syndrome and lung syndrome. By using the 4 techniques of enzyme addhesion ELISA, immue fluorescemce, and western blot, 61 samply of serum of Rattus Novergicus, in Thanh Hoa, year 2003, ELISA rat serum was (+) and 9/61 were suspected. More over, by immunofluoresceime and western blot, antihantavirus antibody was confirmed on 6/9 samples. These 6 sample from the rats, were studied by WB technique with specific serum SEO, in 2 samples, 51 KDa band was found. Results concluded that in Rattus Novergicus from Thanh Hoa, anti Hantaan virus antibody was detected.