RESUMEN
AIM: Determination of serogroup and PCR-genotyping of Vibrio cholerae non-O1/non-O139 strains isolated from surface basins and sewages of Rostov-on-Don city in 2003 - 2008. MATERIALS AND METHODS: Seven hundred strains of V. cholerae non-O1/non-O139 serogroups were studied in reaction of slide-agglutination with array of 80 diagnostic sera for non-O1/non-O139 serogroups. Selective screening of strains representing dominating serogroups was performed for extended number of genetic determinants of pathogenicity factors. RESULTS: It was established that V. cholerae belonging to serogroups O53, O67, O75, and O76 are dominating in water ecosystems of Rostov-on-Don city at this time. All studied strains were characterized by lack of cholera toxin genes and toxin-coregulated pili but had different combinations of genes of additional virulence factors. There was no correlation between genotypic characteristics and serogroup. CONCLUSION: The study showed that change of serologic landscape of V. cholerae non-O1/non-O139 occurred in water objects in studied area during last decades. Necessity of dynamic surveillance for circulation of V. cholerae non-O1/non-O139 in aquatic environment with widening of studied spectrum of their biological features was demonstrated.
Asunto(s)
Cólera/virología , Monitoreo del Ambiente , Vibrio cholerae no O1/clasificación , Vibrio cholerae/clasificación , Microbiología del Agua , Cólera/epidemiología , Toxina del Cólera/genética , Monitoreo Epidemiológico , Fimbrias Bacterianas/genética , Genes Bacterianos , Humanos , Federación de Rusia/epidemiología , Serotipificación , Vibrio cholerae/genética , Vibrio cholerae/aislamiento & purificación , Vibrio cholerae O139/clasificación , Vibrio cholerae O139/genética , Vibrio cholerae O139/aislamiento & purificación , Vibrio cholerae no O1/genética , Vibrio cholerae no O1/aislamiento & purificación , Factores de Virulencia/genéticaRESUMEN
The results of the serotyping of 244 V. cholerae non O1/O139 cultures isolated from patients in Uzbekistan in 2000 and 2001 are presented. All isolates were studied by the method of molecular probing and in the polymerase chain reaction for the presence of virulence genes and for sensitivity to phages ctx+, ctx- and hemolytic activity. The use of monoreceptor O-sera O2-O83 made it possible to determine vibrios of 32 serogroups with the dominating role in the etiology of acute enteric diseases belonging to serogroups O18, O62, O82, O37. Genes ctx AB were detected in none of the isolates, 5 of them contained gene tcp A. A group of cultures, sensitive to phage ctx+ and belonging mainly to enteropathogenic serogroups, was detected.