RESUMEN
Fossils of soft tissues provide important records of early animals and embryos, and there is substantial evidence for a role for microbes in soft tissue fossilization. We are investigating the initial events in interactions of bacteria with freshly dead tissue, using marine embryos as a model system. We previously found that microbial invasion can stabilize embryo tissue that would otherwise disintegrate in hours or days by generating a bacterial pseudomorph, a three dimensional biofilm that both replaces the tissue and replicates its morphology. In this study, we sampled seawater at different times and places near Sydney, Australia, and determined the range and frequency of different taphonomic outcomes. Although destruction was most common, bacteria in 35% of seawater samples yielded morphologypreserving biofilms. We could replicate the taphonomic pathways seen with seawater bacterial communities using single cultured strains of marine gammaproteobacteria. Each given species reproducibly generated a consistent taphonomic outcome and we identified species that yielded each of the distinct pathways produced by seawater bacterial communities. Once formed,bacterial pseudomorphs are stable for over a year and resist attack by other bacteria and destruction by proteases and other lytic enzymes. Competition studies showed that the initial action of a pseudomorphing strain can be blocked by a strain that destroys tissues. Thus embryo preservation in nature may depend on contingent interactions among bacterial species that determine if pseudomorphing occurs.We used Artemia nauplius larvae to show that bacterial biofilm replacement of tissue is not restricted to embryos, but is relevant for preservation of small multicellular organisms. We present a model for bacterial selfassembly of largescale threedimensional tissue pseudomorphs, based on smallscaleinteractions among individual bacterial cells to form local biofilms at structural boundaries within the tissue. Localbiofilms then conjoin to generate the pseudomorph.
Asunto(s)
Bacterias/metabolismo , Biopelículas , Fósiles , Agua de Mar/microbiología , Aerobiosis , Anaerobiosis , Animales , Artemia/fisiología , Fenómenos Fisiológicos Bacterianos , Evolución Biológica , Citoplasma/metabolismo , Gammaproteobacteria/metabolismo , Larva , Lípidos/análisis , Erizos de Mar/fisiología , Microbiología del AguaRESUMEN
Axonemes of motile eukaryotic cilia and flagella have a conserved structure of nine doublet microtubules surrounding a central pair of microtubules. Outer and inner dynein arms on the doublets mediate axoneme motility [1]. Outer dynein arms (ODAs) attach to the doublets at specific interfaces [2-5]. However, the molecular contacts of ODA-associated proteins with tubulins of the doublet microtubules are not known. We report here that attachment of ODAs requires glycine 56 in the beta-tubulin internal variable region (IVR). We show that in Drosophila spermatogenesis, a single amino acid change at this position results in sperm axonemes markedly deficient in ODAs. Moreover, we found that axonemal beta-tubulins throughout the phylogeny have invariant glycine 56 and a strongly conserved IVR, whereas nonaxonemal beta-tubulins vary widely in IVR sequences. Our data reveal a deeply conserved physical requirement for assembly of the macromolecular architecture of the motile axoneme. Amino acid 56 projects into the microtubule lumen [6]. Imaging studies of axonemes indicate that several proteins may interact with the doublet-microtubule lumen [3, 4, 7, 8]. This region of beta-tubulin may determine the conformation necessary for correct attachment of ODAs, or there may be sequence-specific interaction between beta-tubulin and a protein involved in ODA attachment or stabilization.
Asunto(s)
Secuencia de Aminoácidos , Axonema/ultraestructura , Drosophila/fisiología , Dineínas/metabolismo , Espermatogénesis/fisiología , Tubulina (Proteína)/química , Animales , Axonema/metabolismo , Drosophila/metabolismo , Drosophila/ultraestructura , Glicina/química , Masculino , Datos de Secuencia Molecular , Conformación Proteica , Espermatozoides/metabolismo , Espermatozoides/ultraestructura , Tomografía Computarizada por Rayos X , Tubulina (Proteína)/genética , Tubulina (Proteína)/metabolismoRESUMEN
Fossilized embryos with extraordinary cellular preservation appear in the Late Neoproterozoic and Cambrian, coincident with the appearance of animal body fossils. It has been hypothesized that microbial processes are responsible for preservation and mineralization of organic tissues. However, the actions of microbes in preservation of embryos have not been demonstrated experimentally. Here, we show that bacterial biofilms assemble rapidly in dead marine embryos and form remarkable pseudomorphs in which the bacterial biofilm replaces and exquisitely models details of cellular organization and structure. The experimental model was the decay of cleavage stage embryos similar in size and morphology to fossil embryos. The data show that embryo preservation takes place in 3 distinct steps: (i) blockage of autolysis by reducing or anaerobic conditions, (ii) rapid formation of microbial biofilms that consume the embryo but form a replica that retains cell organization and morphology, and (iii) bacterially catalyzed mineralization. Major bacterial taxa in embryo decay biofilms were identified by using 16S rDNA sequencing. Decay processes were similar in different taphonomic conditions, but the composition of bacterial populations depended on specific conditions. Experimental taphonomy generates preservation states similar to those in fossil embryos. The data show how fossilization of soft tissues in sediments can be mediated by bacterial replacement and mineralization, providing a foundation for experimentally creating biofilms from defined microbial species to model fossilization as a biological process.
Asunto(s)
Bacterias/crecimiento & desarrollo , Biopelículas , Evolución Biológica , Embrión no Mamífero/microbiología , Fósiles , Aerobiosis , Anaerobiosis , Animales , Anthocidaris/embriología , Autólisis , Bacterias/genética , ADN Bacteriano , Embrión no Mamífero/ultraestructura , Microscopía Electrónica , MineralesRESUMEN
Adult echinoderms possess a highly diverged, pentaradial body plan. Developmental mechanisms underlying this body plan are completely unknown, but are critical in understanding how echinoderm pentamery evolved from bilateral ancestors. These mechanisms are difficult to study in indirect-developing species; in this study, we use the direct-developing sea urchin Heliocidaris erythrogramma, whose accelerated adult development can be perturbed by NiCl(2). We introduce a new nomenclature for the adult echinoderm axes to facilitate discussion of the radially symmetric body plan and the events required to pattern it. In sea urchins, the adult oral-aboral axis is often conflated with the long axes of the five rays; we identify these as distinct body axes, the proximodistal (PD). In addition, we define a circular axis, the circumoral (CO), along which the division into five sectors occurs. In NiCl(2)-treated larvae, aspects of normal PD pattern were retained, but CO pattern was abolished. Milder treatments resulted in relatively normal juveniles ranging from biradial to decaradial. NiCl(2) treatment had no effect either on mesodermal morphology or on the ectodermal gene expression response to an inductive mesodermal signal. This suggests that the mesoderm does not mediate the disruption of CO patterning by NiCl(2). In contrast, mesodermal signaling may explain the presence of PD pattern in treated larvae. However, variations in appendage pattern suggest that ectodermal signals are also required. We conclude that CO patterning in both germ layers is dependent on ectodermal events and PD patterning is controlled by mutual ectoderm-mesoderm signaling.
Asunto(s)
Erizos de Mar/embriología , Animales , Tipificación del Cuerpo , Embrión no Mamífero , Regulación del Desarrollo de la Expresión Génica , Erizos de Mar/genética , Erizos de Mar/metabolismo , Transducción de SeñalRESUMEN
Using Drosophila spermatogenesis as a model, we show that function of the beta-tubulin C-terminal tail (CTT) is not independent of the body of the molecule. For optimal microtubule function, the beta-tubulin CTT and body must match. beta2 is the only beta-tubulin used in meiosis and spermatid differentiation. beta1-tubulin is used in basal bodies, but beta1 cannot replace beta2. However, when beta1 is co-expressed with beta2, both beta-tubulins are equally incorporated into all microtubules, and males exhibit near wild type fertility. In contrast, co-expression of beta2beta1C and beta1beta2C, two reciprocal chimeric molecules with bodies and tails swapped, results in defects in meiosis, cytoskeletal microtubules, and axonemes; males produce few functional sperm and few or no progeny. In these experiments, all the same beta-tubulin parts are present, but unlike the co-assembled native beta-tubulins, the "trans" configuration of the co-assembled chimeras is poorly functional. Our data thus reveal essential intra-molecular interactions between the CTT and other parts of the beta-tubulin molecule, even though the CTT is a flexible surface feature of tubulin heterodimers and microtubules. In addition, we show that Drosophila sperm tail length depends on the total tubulin pool available for axoneme assembly and spermatid elongation. D. melanogaster and other Drosophila species have extraordinarily long sperm tails, the length of which is remarkably constant in wild type flies. We show that in males of experimental genotypes that express wild type tubulins but have half the amount of the normal tubulin pool size, sperm tails are substantially shorter than wild type.
Asunto(s)
Axonema/metabolismo , Espermatogénesis , Espermatozoides/fisiología , Tubulina (Proteína)/metabolismo , Secuencia de Aminoácidos , Animales , Axonema/ultraestructura , Citoesqueleto/metabolismo , Drosophila melanogaster/fisiología , Masculino , Microscopía Electrónica de Transmisión , Datos de Secuencia Molecular , Espermatogénesis/genética , Espermatozoides/ultraestructura , Tubulina (Proteína)/genéticaRESUMEN
To understand the role of body axes in the evolution of larval form, we use the two sea urchins in the genus Heliocidaris, which have distinctly different larval morphologies. Heliocidaris tuberculata is an indirect-developing sea urchin, which forms a pluteus larva, whereas its sister species, Heliocidaris erythrogramma, exhibits direct development and forms a nonfeeding, ovoid larva. Changes along all three larval axes underlie the differences in larval form associated with each developmental mode. Nodal signaling has recently been implicated as important in establishing the dorsal-ventral (D-V) and left-right (L-R) axes in the indirect-developing sea urchin Paracentrotus lividus. However, because of changes in morphology and timing of morphogenetic events associated with the D-V and L-R axes, respectively, in H. erythrogramma, it was unclear whether nodal played the same roles during direct development. We show that the expression patterns and functions of nodal during H. erythrogramma development are similar to its roles in indirect-developing sea urchins in both D-V and L-R axes formation. However, there are profound changes in gene expression downstream of nodal signaling along the D-V axis and major heterochronies in the execution of the function of nodal along the L-R axis. These highly modified events are linked to the dramatic modifications of larval morphology that have occurred during the evolution of direct development in H. erythrogramma.
Asunto(s)
Anthocidaris/crecimiento & desarrollo , Anthocidaris/metabolismo , Evolución Biológica , Regulación del Desarrollo de la Expresión Génica , Proteína Nodal/genética , Proteína Nodal/metabolismo , Animales , Anthocidaris/genética , Proteína Goosecoide/metabolismo , Hibridación in Situ , Larva/anatomía & histología , Larva/crecimiento & desarrollo , Fenotipo , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa , Transducción de Señal , Factores de TiempoRESUMEN
During the development of the compound eye of Drosophila several signaling pathways exert both positive and inhibitory influences upon an array of nuclear transcription factors to produce a near-perfect lattice of unit eyes or ommatidia. Individual cells within the eye are exposed to many extracellular signals, express multiple surface receptors, and make use of a large complement of cell-subtype-specific DNA-binding transcription factors. Despite this enormous complexity, each cell will make the correct developmental choice and adopt the appropriate cell fate. How this process is managed remains a poorly understood paradigm. Members of the CREB binding protein (CBP)/p300 family have been shown to influence development by (1) acting as bridging molecules between the basal transcriptional machinery and specific DNA-binding transcription factors, (2) physically interacting with terminal members of signaling cascades, (3) acting as transcriptional coactivators of downstream target genes, and (4) playing a key role in chromatin remodeling. In a screen for new genes involved in eye development we have identified the Drosophila homolog of CBP as a key player in both eye specification and cell fate determination. We have used a variety of approaches to define the role of CBP in eye development on a cell-by-cell basis.
Asunto(s)
Proteínas de Drosophila , Drosophila melanogaster/genética , Ojo/metabolismo , Regulación del Desarrollo de la Expresión Génica , Proteínas Nucleares/metabolismo , Transactivadores/metabolismo , Animales , Animales Modificados Genéticamente/genética , Animales Modificados Genéticamente/metabolismo , Proteína de Unión a CREB , Cromatina/genética , Cromatina/metabolismo , Drosophila melanogaster/embriología , Drosophila melanogaster/metabolismo , Embrión no Mamífero/metabolismo , Desarrollo Embrionario , Ojo/anatomía & histología , Ojo/crecimiento & desarrollo , Femenino , Masculino , Proteínas Nucleares/genética , Transactivadores/genéticaRESUMEN
Drosophila melanogaster sperm tubulins are posttranslationally glutamylated and glycylated. We show here that axonemes are the substrate for these tubulin C-terminal modifications. Axoneme architecture is required, but full length, motile axonemes are not necessary. Tubulin glutamylation occurs during or shortly after assembly into the axoneme; only glutamylated tubulins are glycylated. Tubulins in other testis microtubules are not modified. Only a small subset of total Drosophila sperm axoneme tubulins have these modifications. Biochemical fractionation of Drosophila sperm showed that central pair and accessory microtubules have the majority of poly-modified tubulins, whereas doublet microtubules have only small amounts of mono- and oligo-modified tubulins. Glutamylation patterns for different beta-tubulins experimentally assembled into axonemes were consistent with utilization of modification sites corresponding to those identified in other organisms, but surrounding sequence context was also important. We compared tubulin modifications in the 9 + 9 + 2 insect sperm tail axonemes of Drosophila with the canonical 9 + 2 axonemes of sperm of the sea urchin Lytichinus pictus and the 9 + 0 motile sperm axonemes of the eel Anguilla japonica. In contrast to Drosophila sperm, L. pictus sperm have equivalent levels of modified tubulins in both doublet and central pair microtubule fractions, whereas the doublets of A. japonica sperm exhibit little glutamylation but extensive glycylation. Tubulin C-terminal modifications are a prevalent feature of motile axonemes, but there is no conserved pattern for placement or amount of these
Asunto(s)
Axonema/fisiología , Drosophila melanogaster/fisiología , Microtúbulos/química , Cola del Espermatozoide/ultraestructura , Espermatozoides/fisiología , Tubulina (Proteína)/metabolismo , Anguilla , Animales , Axonema/ultraestructura , Ácido Glutámico/metabolismo , Masculino , Microscopía Electrónica de Transmisión , Microtúbulos/ultraestructura , Erizos de Mar , Cola del Espermatozoide/química , Cola del Espermatozoide/fisiología , Espermatozoides/ultraestructura , Tubulina (Proteína)/química , Tubulina (Proteína)/aislamiento & purificaciónRESUMEN
The relationship between the primary structure of the beta-tubulin C-terminal tail (CTT) and axoneme structure and function is explored using the spermatogenesis-specific beta2-tubulin of Drosophila. We previously showed that all beta-tubulins used for motile 9 + 2 axonemes contain a conserved sequence motif in the proximal part of the CTT, the beta-tubulin axoneme motif. The differential ability of tubulin isoforms and abilities of beta2-tubulin C-terminal truncations to form axonemes led us to hypothesize that the axoneme motif is essential for axoneme formation and the distal half of the CTT was less important. The studies we report here indicate that it is not that simple. Unexpectedly, some changes in the core sequence of the axoneme motif did not disrupt formation of motile axonemes. And, while deletion of the distal CTT did not disrupt the ability to produce functional sperm [Popodi et al., Cell Motil Cytoskeleton 2005;62:48-64], changing the amino acid sequence in this region can. Thus both regions are important. The deep conservation of the axoneme motif in all eukaryotic groups implies that the presence of the sequence motif confers a functional advantage. The central pair is the axoneme structure most sensitive to perturbations in tubulin molecules; we hypothesize central pair assembly is facilitated by the presence of this motif. Our data reveal that beta2-tubulin has robust properties for axoneme assembly, and that axonemal specializations are embedded in both the CTT and the body of the beta2 molecule.
Asunto(s)
Axonema/metabolismo , Drosophila melanogaster/metabolismo , Tubulina (Proteína)/metabolismo , Secuencia de Aminoácidos , Animales , Axonema/ultraestructura , Dimerización , Drosophila melanogaster/citología , Drosophila melanogaster/ultraestructura , Fertilidad , Masculino , Mitosis , Datos de Secuencia Molecular , Proteínas Mutantes/metabolismo , Mutación/genética , Procesamiento Proteico-Postraduccional , Motilidad Espermática , Espermátides/citología , Espermátides/ultraestructura , Testículo/citología , Testículo/ultraestructura , Tubulina (Proteína)/química , Tubulina (Proteína)/ultraestructuraRESUMEN
The recent discovery of apparent fossils of embryos contemporaneous with the earliest animal remains may provide vital insights into the metazoan radiation. However, although the putative fossil remains are similar to modern marine animal embryos or larvae, their simple geometric forms also resemble other organic and inorganic structures. The potential for fossilization of animals at such developmental stages and the taphonomic processes that might affect preservation before mineralization have not been examined. Here, we report experimental taphonomy of marine embryos and larvae similar in size and inferred cleavage mode to presumptive fossil embryos. Under conditions that prevent autolysis, embryos within the fertilization envelope can be preserved with good morphology for sufficiently long periods for mineralization to occur. The reported fossil record exhibits size bias, but we show that embryo size is unlikely to be a major factor in preservation. Under some conditions of death, fossilized remains will not accurately reflect the cell structure of the living organism. Although embryos within the fertilization envelope have high preservation potential, primary larvae have negligible preservation potential. Thus the paleo-embryological record may have strong biases on developmental stages preserved. Our data provide a predictive basis for interpreting the fossil record to unravel the evolution of ontogeny in the origin of metazoans.
Asunto(s)
Embrión no Mamífero/fisiología , Fósiles , Erizos de Mar/embriología , Animales , Muerte , Fertilización , Modelos AnimalesRESUMEN
Stereoblastic embryos from the Doushantuo Formation of China exhibit occasional asynchronous cell division, with diminishing blastomere volume as cleavage proceeded. Asynchronous cell division is common in modern embryos, implying that sophisticated mechanisms for differential cell division timing and embryonic cell lineage differentiation evolved before 551 million years ago. Subcellular structures akin to organelles, coated yolk granules, or lipid vesicles occur in these embryos. Paired reniform structures within embryo cells may represent fossil evidence of cells about to undergo division. Embryos exhibit no evidence of epithelial organization, even in embryos composed of approximately 1000 cells. Many of these features are compatible with metazoans, but the absence of epithelialization is consistent only with a stem-metazoan affinity for Doushantuo embryos.
Asunto(s)
Embrión no Mamífero/citología , Fósiles , Invertebrados/embriología , Animales , Blastómeros/citología , División Celular , Linaje de la Célula , Forma de la Célula , China , Embrión no Mamífero/ultraestructura , Desarrollo Embrionario , Epitelio/embriología , Gástrula/citología , Gástrula/ultraestructura , Microscopía Electrónica de Rastreo , Microscopía Electrónica de Transmisión , Orgánulos/ultraestructura , Tomografía Computarizada por Rayos XRESUMEN
We have used Drosophila testis-specific beta2-tubulin to determine sequence requirements for different microtubules. The beta2-tubulin C-terminal tail has unique sperm-specific functions [Dev Biol 158:267-286 (2003)] and is also important for forming stable heterodimers with alpha-tubulin, a general function common to all microtubules [Mol Biol Cell 12(7):2185-2194 (2001)]. beta-tubulins utilized in motile 9 + 2 axonemes contain a C-terminal sequence "axoneme motif" [Science 275 (1997) 70-73]. C-terminal truncated beta2-tubulin cannot form the sperm tail axoneme. Here we show that a partially truncated beta2-tubulin (beta2Delta7) containing only the proximal portion of the C-terminal tail, including the axoneme motif, can support production of functional motile sperm. We conclude that these proximal eight amino acids specify the binding site for protein(s) essential to support assembly of the motile axoneme. Males that express beta2Delta7, although they are fertile, produce fewer sperm than wild type males. Beta2Delta7 causes a slightly increased error rate in spermatogenesis attributable to loss of stabilizing properties intrinsic to the full-length C-terminal tail. Therefore, beta2Delta7 males would be at a selective disadvantage and it is likely that the full-length C-terminus would be essential in the wild and in evolution.
Asunto(s)
Proteínas de Drosophila/fisiología , Cola del Espermatozoide/fisiología , Tubulina (Proteína)/fisiología , Secuencia de Aminoácidos , Animales , Proteínas de Drosophila/genética , Drosophila melanogaster , Masculino , Microscopía Electrónica , Mutagénesis Sitio-Dirigida , Procesamiento Proteico-Postraduccional , Motilidad Espermática/fisiología , Espermatogénesis/genética , Testículo/metabolismo , Tubulina (Proteína)/genéticaRESUMEN
The Drosophila Centrosomin (Cnn) protein is an essential core component of centrosomes in the early embryo. We have expressed a Cnn-GFP fusion construct in cleavage stage embryos, which rescues the maternal effect lethality of cnn mutant animals. The localization patterns seen with GFP-Cnn are identical to the patterns we see by immunofluorescent staining with anti-Cnn antibodies. Live imaging of centrosomes with Cnn-GFP reveals surprisingly dynamic features of the centrosome. Extracentrosomal particles of Cnn move radially from the centrosome and frequently change their direction. D-TACC colocalized with Cnn at these particles. We have named these extrusions 'flares'. Flares are dependent on microtubules, since disruption of the microtubule array severs the movement of these particles. Movement of flare particles is cleavage-cycle-dependent and appears to be attributed mostly to their association with dynamic astral microtubules. Flare activity decreases at metaphase, then increases at telophase and remains at this higher level of activity until the next metaphase. Flares appear to be similar to vertebrate PCM-1-containing 'centriolar satellites' in their behavior. By injecting rhodamine-actin, we observed that flares extend no farther than the actin cage. Additionally, disruption of the microfilament array increased the extent of flare movement. These observations indicate that centrosomes eject particles of Cnn-containing pericentriolar material that move on dynamic astral microtubules at a rate that varies with the cell cycle. We propose that flare particles play a role in organizing the actin cytoskeleton during syncytial cleavage.
Asunto(s)
Centrosoma/metabolismo , Proteínas de Drosophila , Proteínas de Homeodominio/metabolismo , Animales , División Celular , Centrosoma/química , Drosophila/citología , Drosophila/embriología , Embrión no Mamífero/citología , Embrión no Mamífero/ultraestructura , Microscopía por Video , Microtúbulos/metabolismoRESUMEN
We made hybrid crosses between closely and distantly related sea urchin species to test two hypotheses about the evolution of gene regulatory systems in the evolution of ontogenetic pathways and larval form. The first hypothesis is that gene regulatory systems governing development evolve in a punctuational manner during periods of rapid morphological evolution but are relatively stable over long periods of slow morphological evolution. We compared hybrids between direct and indirect developers from closely and distantly related families. Hybrids between eggs of the direct developer Heliocidaris erythrogramma and sperm of the 4-million year distant species H. tuberculata, an indirect developer, restored feeding larval structures and paternal gene expression that were lost in the evolution of the direct-developing maternal parent. Hybrids resulting from the cross between eggs of H. erythrogramma and sperm of the 40-million year distant indirect-developer Pseudoboletia maculata are strikingly similar to hybrids between the congeneric hybrids. The marked similarities in ontogenetic trajectory and morphological outcome in crosses of involving either closely or distantly related indirect developing species indicates that their regulatory mechanisms interact with those of H. erythrogramma in the same way, supporting remarkable conservation of molecular control pathways among indirect developers. Second, we tested the hypothesis that convergent developmental pathways in independently evolved direct developers reflect convergence of the underlying regulatory systems. Crosses between two independently evolved direct-developing species from two 70-million year distant families, H. erythrogramma and Holopneustes purpurescens, produced harmoniously developing hybrid larvae that maintained the direct mode of development and did not exhibit any obvious restoration of indirect-developing features. These results are consistent with parallel evolution of direct-developing features in these two lineages.