Rep-PCR Analyses Reveal Genetic Variation of Ralstonia solanacearum Causing Wilt of Solanaceaous Vegetables in Bangladesh.

Ralstonia solanacearum, a soil-borne and seed-borne plant pathogenic bacterium, causes bacterial wilt to several important crop plants causing substantial economic losses. To provide population information on this pathogen for developing effective control strategies, Rep-PCR was used to analyze the genetic variation of 18 representative isolates of R. solanacearum collected in Bangladesh. Phenotypic analyses revealed that all eighteen isolates belong to biotype 3 with wide diversity in aggressiveness on eggplant, tomato, and chili. Rep-PCR studies utilizing the REP, ERIC, and BOXIR primers showed a wide variation at the genetic level among the R. solanacearum isolates used in this study. Dendrogram constructed using REP, ERIC, and BOXIR primers based on banding patterns implied that R. solanacearum isolates were genetically diversified and distributed in four clusters at 83%, 80%, and 63% similarity index, respectively. The genetic relationship assayed by rep-PCR highlighted a wide range of genetic variation but no relation among geographical origin, aggressiveness, and phylogenetic groups of R. solanacearum isolates. These results conceded that other molecular markers related to virulence gene(s) might reveal the complex relationship among geographical origin, aggressiveness, and phylogenetic groups.

Reducing stress concentration on the cup rim of hip implants under edge loading.

High stress concentration under edge loading on the cup rim contact due to micro-separation causes accelerated striping wear, fracture, and fatigue in hip implant components. While continuous effort is devoted into improving bearing design and surgical procedure to tackle the problem, the concern still has remained forcing biomedical engineers to seek for new and alternative solutions. The current paper aims to investigate the effect of a new geometry "spline" introduced at the cup's rim corner to minimise stress concentration under edge loading. Three-dimensional finite element modelling of a metal-on-metal hip implant is developed, where contact pressure, von Mises stress, and strain are predicted for three spline geometries, ie, equivalent characteristic arc radius (R = 0.5, 1.0, and 1.5 mm) at four micro-separations (of 1.0, 1.5, 2.0, and 2.5 mm) simulating edge loading on the rim contact via the application of a constant vertical load of 3 kN. The efficacy of the spline is compared with that of circular arc and sharp corner (ie, no arc) geometries. Overall, the spline outperforms both sharp corner and circular arc in reducing contact pressure, stress, and strain. The benefit of the spline over the circular arc is quite promising at larger micro-separation but fairly marginal at smaller arc radius and micro-separation. The findings indicate that, as an alternative to the circular fillet, the spline can be considered a potential geometry to be incorporated at the rim corner of the cup.