[Proliferative activity of rat skin fibroblasts under the action of glucocorticoids and gestagens].

Temporal and concentration dependences of the effect of the new synthetic gestagen 17alpha-acetoxy-3beta-butanoyloxy-6-methyl-pregna-4,6-dien-20-one (ABMP) and reference glucocorticoids (dexamethasone and hydrocortisone) and progesterone on the proliferative activity of rat skin fibroblasts were examined using the MTT assay. The results demonstrated that ABMP along with glucocorticoids produced an antiproliferative action against fibroblasts, with the maximum (38%) suppression of the metabolic activity of cells observed after 5-day incubation at a drug concentration of 10(-5) M.

[Antistaphylococcal activity of the complex of natural cytokines]. / Protivostafilokokkovoe deistvie kompleksa prirodnykh tsitokinov.

The in vitro study of the action of the complex of natural cytokines (CNC), or preparation Superlymph, on different microbial test strains (Staphylococcus aureus, Escherichia coli, yeast-like fungi of the genus Candida) was carried out. CNC suppressed the growth of S. aureus test culture, depending on the concentration of the preparation. The inhibiting effect was observed in the presence of Ca and Mg bivalent cations in the medium. Under the chosen conditions of the experiment CNC did not inhibit the growth of E. coli test cultures, as well as test cultures of yeast-like fungi of the genus Candida. In addition to its antibacterial effect, Superlymph also produced some effect on the release of cathepsin G, the lysosomal enzyme of human leukocyte granules.

[Estradiol binding and lipid metabolism in the plasma membrane of myometrial cells during tumor growth]. / Sviazyvanie éstradiola i metabolizm lipidov v plazmaticheskikh membranakh kletok miometriia pri opukholevom roste.

The study was concerned with evaluation of the parameters of specific binding of 17-beta-estradiol to myometrial plasma membrane in tumor growth versus lipid profile of the membrane and A2 and C phospholipase activity. Tumor growth was shown to involve an increase in the association constant of plasma membrane, changes in the lipid profile and activity of the enzymes. The role of these factors in myometrial carcinogenesis is discussed.

[Steroid hormones and lysosomes]. / Steroidnye gormony i lizosomy.

The data on lysosomotropic effect of steroid hormones are reviewed. The effect of steroid hormones correlated with the type of tissues and dose of hormones. A possible role of lysosomes in realization of hormonal signal in the cell biological response is discussed.

Activity lysosomal enzymes in rat skin fibroblasts after treatment with progesterone and new gestagen ABMP.

The effects of a new synthetic gestagen 17alpha-acetoxy-3beta-butanoyloxy-6-methyl-pregna-4,6-dien-20-on (ABMP) and reference drug progesterone on rat skin fibroblasts were evaluated by variations in lysosomal enzyme activity (cathepsin D and beta-glucosidase). Our results suggest that ABMP exhibits lysosomotropic properties, which depended on its concentration and time of treatment. The direct effect of progesterone on lysosomal enzyme activity in skin fibroblasts was compared to the influence of systemic treatment with gestagens on skin lysosomes. The data indicate that local application of gestagen preparations holds much promise for the therapy of skin diseases accompanied by increased proliferation (e.g. psoriasis).

[Effect of estradiol on lipid peroxidation in skin]. / Vliianie éstradiola na perekisnoe okislenie lipidov v kozhe.

The effects of estradiol on intensity of lipid peroxidation in rats' derma and epiderma have been studied. More high level of lipid peroxidation, correlating with activity of water-solved antioxidants of these tissues in comparison with epidermis has been detected in derma. The level of lipid peroxidation decreased in proportion to hormone concentration when adding estradiol to tissue homogenates in vitro.

[Effect of hydrocortisone on the activity of lysosomal enzymes in skin]. / Vliianie gidrokortizona na aktivnost' lizosomal'nykh fermentov kozhi.

The mechanisms of glucocorticoids' effect on the functional activity of lysosomes in rats dermis and epidermis have been studied. The activity of enzymes in both parts of skin in females was more than in ones in males. Intraperitoneal injection of hydrocortisone doesn't change the functional activity of lysosomes in skin in doses less than 1 mg per 100 g of weight apart from the sex of rats. The hormone in dose 1 mg/100 g of weight causes statistically reliable releasing of lysosomal glycosidases from the epidermis and dermis of both of the sex of rats, and cathepsin D in females as well. In experiments in vitro the concentration of hormone 10(-8) M causes the fall of possibility of enzymatic fission of substrates in dermis, but the concentration 10(-3) M causes the increasing of the one on the contrary. Enzymatic alterations in the results of action of glucocorticoids in dermis and in epidermis as in males so in females of rats and the causes of their distinguishes have been discussed.

[Effect of hydrocortisone on lipid metabolism in the plasma membranes of hepatocytes]. / Vliianie gidrokortizona na metabolizm lipidov v plazmaticheskikh membranakh gepatotsitov.

Lipid composition of cell plasma membranes and hepatocyte lysosome phospholipase activity were studied. The data obtained have shown the phospholipid involvement in the realization of steroid hormone effect on the effector cells. The role of lysosomes in the same process is discussed.

[Lipoperoxidation in eye tissues exposed to hormones]. / Issledovanie perekisnogo okisleniia lipidov v tkaniakh glaza pri gormonal'nykh vozdeistviiakh.

The effect of hormones of varied action spectrum on lipoperoxidation of unsaturated fatty acids in the eye tissues has been studied. Both antioxidant and prooxidant action of diverse hormones on the sclera, cornea and ciliary body has been demonstrated with respect to the hormonal dose, exposure time and other factors. Potential participation of lipoperoxidation processes in the development of ophthalmopatholody is discussed.

[Effect of prostaglandin F2alpha on the lysosomal membranes of eye tissues]. / Vliianie prostaglandina F2alpha na membrany lizosom tkanei glaza

It was demonstrated that both in vitro and in intravenous injection of prostaglandin F2alpha there occurred labilization of the membranes of sclera and ciliary body lysosomes, in difference from the cornea in rabbits. Glycosidase activity appeared in the vitreous body under the effect of prostaglandin, which was absent under normal conditions.

[The cellular acid phosphatase activity in yeast-like fungi of the genus Candida exposed to ultrasound, polyene antibiotics and dyes]. / Izuchenie aktivnosti kisloi fosfatazy kletok drozhzhepodobnykh gribov roda Candida pri vozdeistvii na nikh ul'trazvuka, polienovykh antibiotikov i krasitelei.

The activity of one of the lysosomal membrane marker enzymes--acid phosphatase from the Candida yeast fungi on their exposure to ultrasound (US), polyenic antibiotics (amphotericin B and nystatin) dye antiseptics (ethacridine lactate, methylene blue), and their combinations was assayed. The impact of US and the drugs, in particular their combination, was found to be followed by activation of the fungal lysosomal apparatus function and increases in their catabolic processes. The highest rise in lysosomal catabolic activity was found when the polyenic antibiotics were used in combination with US, which reflects the higher damaging effect of this combination against Candida lysosomal membranes than the dyes and of these antibiotics and US alone. The studies provide strong evidence for the preference of the combined use of US and the polyenic antibiotics in candidiasis as a factor enhancing their fungicidal effect against Candida yeast fungi.

[Evaluation of the capacity of the thymus to accumulate testosterone and of the nature of its interaction with the thymocytes]. / Otsenka sposobnosti timusa akkumulirovat' testosteron i kharaktera vzaimodeistviia ego s timotsitami.

It was found out that thymus accumulated 3H-testosterone from blood at the same degree as liver, prostate and skeletal muscle. However, hormone was quickly eliminated from the thymus and prostate accumulated it. Thymocytes bound about 5% of testosterone accumulated by the organ. The dependence of binding of 3H-testosterone by thymocytes in vitro and the absence of competition between labelled and unlabelled forms of hormone were revealed. The results obtained make it possible to conclude that thymocytes lack the systems of specific binding of androgen.

[Responses of different types of connective tissue to hormone administration]. / Reaktsiia raznykh vidov soedinitel'noi tkani na vvedenie gormonov.

The lysosomal glycosidase activity of the eye tissues (the sclera and cornea), the bone tissues and cartilage were studied. The intraperitoneal injection of tyrocalcitonine (TCT), deoxycorticosterone (DOCS), hydrocortisone (HC), and somatotropic hormone (STH) influenced both the activity of beta-galactosidase, beta-glucosidase, and hyaluronidase, the the functional state of thy lysosomal membranes of the connective tissues under investigation. GC and STH caused stabilization, whereas DOCS and large doses of TCT--a labilizing effect on the lysosomal membranes and tissues understudy. The absolute activity of the enzymes in the homogenates decreased after the HC and STH injection. DOCS produced an opposite effect.

[Enzymes of cyclic nucleotide degradation in eye tissues during experimental ocular herpes]. / Fermenty raspada tsiklicheskikh nukleotidov v tkaniakh glaza pri éksperimental'nom oftal'mpgerpese.

Activity of phosphodiesterases disintegrating cAMP and cGMP in the cornea, sclera and ciliary body was investigated in health and in different stages of experimental herpetic keratitis. The problems concerning the role of the cyclase system in the pathogenesis of herpetic keratitis and the possibility of applying some of the drugs to the disease treatment are discussed.

[Incorporation of labeled cortisol into different types of connective tissue]. / Vkliuchenie mechenogo kortizola v raznye vidy soedinitel'noi tkani.

The properties of early (BFUen) and late (CFUen) erythropoietin-independent progenitors were studied in bone marrow plasma clot cultures of mice. Syngeneic serum was used as a stimulant of colony formation. It was revealed that serum from polycytemic mice did not alter the efficacy of cloning the erythroid progenitors. Varying effects produced on erythropoiesis in vivo did not significantly change the number of CFUen and BFUen, with the exception of posttransfusion polycytemia where the number of CFUen showed a statistically significant rise. Assessment of radiosensitivity and proliferative activity of the erythroid progenitors showed that for CFUen D0=206 rad and n=1.3; for BFUen D0=118 rad and n=1.4; the proportion of proliferating CFUen was 42% +/- 12.5 and that of BFUen 46% +/- 4.5. The data obtained confirm the hypothesis of the erythropoietin-independent nature of the erythroid progenitors expressed under the culture conditions used.

[Distribution of labeled cortisol in the tissues and media of the eye]. / Raspredelenie mechenogo kortizola v tkaniakh i sredakh glaza

Incorporation of cortizol-3H and its uptake by the tissues and media of the eye was studied to expose the target tissue for the given hormone. Cortizol distribution was investigated in the sclera, ciliary body, cornea, iris, the lens capsule, aqeous humour, and the vitreous body. It was shown that the eye tissues and media had different intensity of cortizol incorporation, as well as different character of its elimination. The sclera, cornea, ciliary body and the lens capsule proved to be the target tissues for cortizol.

[The effect of ultrasound, the polyene antibiotic amphotericin B and their combination on the biomembranes of yeast-like fungi in the genus Candida]. / Vliianie ul'trazvuka, polienovogo antibiotika amfoteritsina B i ikh sochetaniia na biomembrany drozhzhepodobnykh grinov roda Candida.

The effect of ultrasound (US) and the antibiotic amphotericin (A-B) on the structure of Candida albicans membranes. The most expression of destructive processes and appearance of free radicals in mycetic cells was observed in combination of US and A-B by chemiluminescence and ultrastructural analyses. The mechanisms of action of US and A-B on mycetic cells were discussed in the article. The expedience of the combination of US and A-B in candidiasis as a factor, increasing fungicidal effect on the candidiasis agent of Candida albicans.

[The lipid spectrum of the skin in psoriasis]. / Lipidnyi spektr kozhi pri psoriaze.

The study was undertaken to examine the lipid composition of and the activity of phospholipase A2 in the skin of patients with psoriasis. There were increases in the levels of phosphatidylcholine, phosphatidylethanolamine and the activity of phospholipase A2 in psoriatic papule as compared to the normal skin. A possible role of glucocorticoids and the revealed abnormalities in the pathogenesis of psoriasis is discussed.

[Effect of dexamethasone on skin phospholipid spectrum in laboratory animals]. / Vliianie deksametazona na fosfolipidnyi spektr kozhi laboratornykh zhivotnykh.

The effect of dexamethasone on content of phospholipids in rats' and rabbits' skin has been studied. It has been shown that the present of phospholipids in total content of lipids was more in skin of rats than skin of rabbits. Phosphocholine and phosphatidylethanolamine predominated among phospholipids in human skin as well. Dexamethasone changed both the total content of phospholipids and its fractions. The role of observed alterations to the regulation of cell process has been discussed.

[Corneal lysosomes in experimental ophthalmoherpes]. / Lizosomy rogovitsy pri éksperimental'nom oftal'mogerpese.

Activity and intracellular distribution of lysosomal glucosidases (beta-glucosidase and beta-galactosidase) of the cornea as well as membrane permeability were studied in experimental herpetic keratitis. It was demonstrated that the action of herpes simplex entailed labilization of the lysosomal membranes of the cornea together with the decreased strength of lysosomal association with the enzymes thereby favouring the increased enzyme-substrate contact and excess destruction of cellular and exocellular glycosaminoglycans. The data obtained suggest that lysosomes participate in the pathogenesis of herpetic keratitis.