RESUMEN
BACKGROUND & AIMS: Platelet-derived growth factor (PDGF) is the most potent stimulus for proliferation and migration of stellate cells. PDGF receptor ß (PDGFRß) expression is an important phenotypic change in myofibroblastic cells that mediates proliferation and chemotaxis. Here we analyzed the relationship between PDGFRß expression, hemodynamic deterioration, and fibrosis in CCl(4)-treated rats. Thereafter, we investigated the effects produced by an adenovirus encoding a dominant-negative soluble PDGFRß (sPDGFRß) on hemodynamic parameters, PDGFRß signaling pathway, and fibrosis. METHODS: Mean arterial pressure, portal pressure, PDGFRß mRNA expression, and hepatic collagen were assessed in 6 controls and 21 rats induced to hepatic fibrosis/cirrhosis. Next, 30 fibrotic rats were randomized into three groups receiving iv saline and an adenovirus encoding for sPDGFRß or ß-galactosidase. After 7days, mean arterial pressure, portal pressure, serum sPDGFRß, and hepatic collagen were measured. RESULTS: CCl(4)-treated animals for 18weeks showed a significantly higher increase in PDGFRß mRNA compared to those treated for 13weeks and control rats. In CCl(4)-treated rats, the fibrous tissue area ranged from moderate to severe fibrosis. A direct relationship between the degree of fibrosis, hemodynamic changes, and PDGFRß expression was observed. Fibrotic rats transduced with the adenovirus encoding sPDGFRß showed increased mean arterial pressure, decreased portal pressure, lower activation of the PDGFRß signaling pathway, and reduced hepatic collagen than fibrotic rats receiving ß-galactosidase or saline. CONCLUSIONS: PDGFRß activation closely correlates with hemodynamic disorders and increased fibrosis in CCl(4)-treated rats. Adenoviral dominant negative soluble PDGFRß improved fibrosis. As a result, the hemodynamic abnormalities were ameliorated.
Asunto(s)
Adenoviridae/genética , Colágeno/metabolismo , Hemodinámica/fisiología , Cirrosis Hepática/metabolismo , Cirrosis Hepática/fisiopatología , Hígado/metabolismo , Presión Portal/fisiología , Receptor beta de Factor de Crecimiento Derivado de Plaquetas/metabolismo , Actinas/metabolismo , Animales , Tetracloruro de Carbono/efectos adversos , Modelos Animales de Enfermedad , Progresión de la Enfermedad , Técnicas In Vitro , Hígado/irrigación sanguínea , Cirrosis Hepática/inducido químicamente , Masculino , Ratas , Ratas Wistar , Receptor beta de Factor de Crecimiento Derivado de Plaquetas/genética , Transducción de Señal/fisiología , Transducción Genética , beta-Galactosidasa/genética , beta-Galactosidasa/metabolismoRESUMEN
Noncollinear second harmonic generation from a Bacteriorhodopsin (BR) oriented multilayer film was systematically investigated by varying the polarization state of both fundamental beams. Both experimental results and theoretical simulations, show that the resulting polarization mapping is an useful tool to put in evidence the optical chirality of the investigated film as well as the corresponding multipolar contributions to the nonlinear.
Asunto(s)
Bacteriorodopsinas/química , Bacteriorodopsinas/ultraestructura , Membranas Artificiales , Modelos Biológicos , Modelos Químicos , Simulación por Computador , Luz , Dinámicas no Lineales , Refractometría , Dispersión de RadiaciónRESUMEN
The kinetics of electrogenic events associated with the different steps of the light-induced proton pump of bacteriorhodopsin is well studied in a wide range of time scales by direct electric methods. However, the investigation of the fundamental primary charge translocation phenomena taking place in the functional energy conversion process of this protein, and in other biomolecular assemblies using light energy, has remained experimentally unfeasible because of the lack of proper detection technique operating in the 0.1- to 20-THz region. Here, we show that extending the concept of the familiar Hertzian dipole emission into the extreme spatial and temporal range of intramolecular polarization processes provides an alternative way to study ultrafast electrogenic events on naturally ordered biological systems. Applying a relatively simple experimental arrangement based on this idea, we were able to observe light-induced coherent terahertz radiation from bacteriorhodopsin with femtosecond time resolution. The detected terahertz signal was analyzed by numerical simulation in the framework of different models for the elementary polarization processes. It was found that the principal component of the terahertz emission can be well described by excited-state intramolecular electron transfer within the retinal chromophore. An additional slower process is attributed to the earliest phase of the proton pump, probably occurring by the redistribution of a H bond near the retinal. The correlated electron and proton translocation supports the concept, assigning a functional role to the light-induced sudden polarization in retinal proteins.
Asunto(s)
Bacteriorodopsinas/química , Electrones , Halobacterium salinarum/química , Protones , Radiación , Simulación por Computador , Transporte de Electrón , Cinética , Óptica y Fotónica , Bombas de Protones , Factores de TiempoRESUMEN
BACKGROUND & AIMS: Increased activity of the vascular Akt/eNOS signaling pathway is involved in the hemodynamic and renal complications developed by patients and rats with cirrhosis and ascites. This occurs in the setting of impaired Akt/eNOS activity within the cirrhotic liver. Here we assessed the feasibility of selectively inhibiting vascular eNOS without further impairing the intrahepatic activity of this enzyme. Ultimately, we sought to determine whether endothelial transduction of a constitutively inactive mutant of Akt (AA-Akt) improves circulatory function and sodium excretion in cirrhotic rats with ascites. METHODS: First, we administered recombinant adenoviruses that encode the ß-galactosidase gene (ß-gal) to 5 control rats and 5 cirrhotic rats with ascites and analyzed their tissue distribution by chemiluminescence. Next, urine samples were obtained from 18 cirrhotic rats with ascites and then the animal randomly received saline or adenoviruses containing the ß-gal or the AA-Akt genes. Following a 24-h urine collection period, hemodynamic studies were performed and tissue samples were obtained to analyze Akt and eNOS expressions. RESULTS: No ß-gal activity was detected in the liver of cirrhotic rats compared to that of controls. This was paralleled by increased ß-gal activity in other territories such as the thoracic aorta. AA-Akt transduction improved systemic hemodynamics, splanchnic perfusion pressure and renal excretory function in comparison with cirrhotic rats transduced with ß-gal adenoviruses or receiving saline. Moreover, the AA-Akt transgene did not modify portal pressure. CONCLUSIONS: Inactivation of extrahepatic vascular Akt and the concomitant decrease in nitric oxide expression ameliorate systemic hemodynamics and renal excretory function in experimental cirrhosis.
Asunto(s)
Cirrosis Hepática Experimental/enzimología , Cirrosis Hepática Experimental/terapia , Proteínas Proto-Oncogénicas c-akt/antagonistas & inhibidores , Adenoviridae/genética , Animales , Ascitis/etiología , Ascitis/fisiopatología , Bovinos , Células Cultivadas , Células HEK293 , Hemodinámica , Humanos , Circulación Hepática , Cirrosis Hepática Experimental/fisiopatología , Masculino , Proteínas Mutantes/genética , Natriuresis , Óxido Nítrico Sintasa de Tipo III/antagonistas & inhibidores , Proteínas Proto-Oncogénicas c-akt/genética , Proteínas Proto-Oncogénicas c-akt/fisiología , Ratas , Ratas Wistar , Proteínas Recombinantes/genética , Transducción GenéticaRESUMEN
BACKGROUND AND AIMS: The extent and molecular mechanisms governing plasma extravasation and formation of ascites in cirrhosis are unknown. Vascular endothelial growth factor-A (VEGF-A) and angiopoietin-2 (Ang-2) are endogenous substances with powerful vascular permeability effects. We assessed regional blood flow, vascular leakage, mRNA and tissular expression of VEGF-A and Ang-2 and vascular permeability following VEGF receptor 2 blockade in control and cirrhotic rats to define the vascular territories showing altered vascular permeability in cirrhosis and to determine whether VEGF-A and Ang-2 are involved in this phenomenon. METHODS: Arterial blood flow was analysed with the coloured microsphere method. Vascular leakage was measured and visualised with the dye Evan's Blue and colloidal carbon techniques, respectively. VEGF-A and Ang-2 expression were determined by real-time polymerase chain reaction (RT-PCR), immunohistochemistry and western blot. The effect on vascular permeability induced by VEGFR(2) blockade was assessed by administration of the receptor inhibitor SU11248. RESULTS: Arterial blood flow was increased in the mesentery, pancreas and small intestine but not in the kidney and spleen of cirrhotic rats as compared to controls. Increased vascular leakage was observed in the mesentery and liver, where colloidal carbon spread from microvessels to the adjacent fibrotic tracts. Increased hepatic and mesenteric expression of VEGF-A and Ang-2 was found in cirrhotic rats as compared to controls. Blockade of VEGFR(2) markedly reduced hepatic and mesenteric vascular leakage in cirrhotic rats. CONCLUSIONS: Enhanced endothelial permeability is restricted to the hepatic and mesenteric vascular beds in cirrhotic rats with ascites and VEGF-A and Ang-2 are key factors in the signalling pathways regulating this dysfunction.
Asunto(s)
Angiopoyetina 2/metabolismo , Cirrosis Hepática/metabolismo , Hígado/metabolismo , Factor A de Crecimiento Endotelial Vascular/metabolismo , Angiopoyetina 1/análisis , Angiopoyetina 2/análisis , Angiopoyetina 2/genética , Animales , Permeabilidad Capilar/efectos de los fármacos , Carbono , Combinación de Medicamentos , Endotelio Vascular/metabolismo , Indoles/farmacología , Hígado/irrigación sanguínea , Masculino , Mesenterio/irrigación sanguínea , Mesenterio/metabolismo , Microvasos , Páncreas/irrigación sanguínea , Páncreas/metabolismo , Povidona , Pirroles/farmacología , ARN Mensajero/análisis , Ratas , Ratas Wistar , Coloración y Etiquetado , Sunitinib , Factor A de Crecimiento Endotelial Vascular/análisis , Factor A de Crecimiento Endotelial Vascular/genética , Receptor 2 de Factores de Crecimiento Endotelial Vascular/análisis , Receptor 2 de Factores de Crecimiento Endotelial Vascular/antagonistas & inhibidoresRESUMEN
The light-activated proton-pumping bacteriorhodopsin and chloride ion-pumping halorhodopsin are compared. They belong to the family of retinal proteins, with 25% amino acid sequence homology. Both proteins have seven alpha helices across the membrane, surrounding the retinal binding pocket. Photoexcitation of all-trans retinal leads to ion transporting photocycles, which exhibit great similarities in the two proteins, despite the differences in the ion transported. The spectra of the K, L, N and O intermediates, calculated using time-resolved spectroscopic measurements, are very similar in both proteins. The absorption kinetic measurements reveal that the chloride ion transporting photocycle of halorhodopsin does not have intermediate M characteristic for deprotonated Schiff base, and intermediate L dominates the process. Energetically the photocycle of bacteriorhodopsin is driven mostly by the decrease of the entropic energy, while the photocycle of halorhodopsin is enthalpy-driven. The ion transporting steps were characterized by the electrogenicity of the intermediates, calculated from the photoinduced transient electric signal measurements. The function of both proteins could be described with the 'local access' model developed for bacteriorhodopsin. In the framework of this model it is easy to understand how bacteriorhodopsin can be converted into a chloride pump, and halorhodopsin into a proton pump, by changing the ion specificity with added ions or site-directed mutagenesis.
Asunto(s)
Bacteriorodopsinas/química , Bombas Iónicas/química , Bombas de Protones/química , Secuencia de Aminoácidos , Cloruros/química , Halorrodopsinas , Datos de Secuencia Molecular , Fotoquímica , Conformación Proteica , Bases de Schiff/química , Alineación de SecuenciaRESUMEN
The consequences of replacing Asp-85 with glutamate in bacteriorhodopsin, as expressed in Halobacterium sp. GRB, were investigated. Similarly to the in vitro mutated and in Escherichia coli expressed protein, the chromophore was found to exist as a mixture of blue (absorption maximum 615 nm) and red (532 nm) forms, depending on the pH. However, we found two widely separated pKa values (about 5.4 and 10.4 without added salt), arguing for two blue and two red forms in separate equilibria. Both blue and red forms of the protein are in the two-dimensional crystalline state. A single pKa, such as in the E. coli expressed protein, was observed only after solubilization with detergent. The photocycle of the blue forms was determined at pH 4.0 with 610 nm photoexcitation, and that of the red forms at pH 10.5 and with 520 nm photoexcitation, in the time-range of 100 ns to 1 s. The blue forms produced no M, but a K- and an L-like intermediate, whose spectra and kinetics resembled those of blue wild-type bacteriorhodopsin below pH 3. The red forms produced a K-like intermediate, as well as M and N. Only the red forms transported protons. Specific perturbation of the neighborhood of the Schiff base by the replacement of Asp-85 with glutamate was suggested by (1) the shift and splitting of the pKa for what is presumably the protonation of residue 85, (2) a 36 nm blue-shift in the absorption of the all-trans red chromophore and a 25 nm red-shift of the 13-cis N chromophore, as compared to wild-type bacteriorhodopsin and its N intermediate, and (3) significant acceleration of the deprotonation of the Schiff base at pH 7, but not of its reprotonation and the following steps in the photocycle.
Asunto(s)
Bacteriorodopsinas/química , Ácido Aspártico/química , Transporte Biológico , Escherichia coli/metabolismo , Glutamatos/química , Ácido Glutámico , Halobacterium/genética , Halobacterium/metabolismo , Concentración de Iones de Hidrógeno , Cinética , Mutación , Fotoquímica , Protones , Espectrofotometría Ultravioleta , Difracción de Rayos XRESUMEN
The kinetics of photoelectric and optical signals were measured on samples containing oriented purple membranes immobilized in a poly(vinyl alcohol) film and on purple membranes introduced into a PVA-H2O mixture. The bacteriorhodopsin photocycle in the PVA-H2O mixture was complete. The only observed changes were the slowing down of the optical and electrical signals in relation to the M412-O640 and O640-bRall-trans steps. In the PVA film the O640 intermediate disappeared and a negative photoelectric signal appeared.
Asunto(s)
Bacteriorodopsinas/química , Halobacterium/análisis , Alcohol Polivinílico/química , Conductividad Eléctrica , Cinética , Luz , Sustancias Macromoleculares , Espectrofotometría , AguaRESUMEN
The light-driven chloride pump, halorhodopsin, binds and transports chloride across the membrane, and to a lesser extent nitrate. Binding and transport kinetics, and resonance Raman spectra of the retinal Schiff base, with these anions suggest the existence of two mutually exclusive binding sites. One of these may be the uptake site, and the other the release site during the transport. Plausible locations can be suggested for these sites, because halorhodopsin is a small protein with few buried positively charged residues, and the primary structure of a second pigment with similar function has recently become available for comparison.
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Bacteriorodopsinas/metabolismo , Cloruros/metabolismo , Canales Iónicos/metabolismo , Proteínas de la Membrana/metabolismo , Transporte Biológico Activo , Canales de Cloruro , Halobacterium/metabolismo , Halorrodopsinas , Nitratos/metabolismoRESUMEN
The light-sensitive protein, bacteriorhodopsin (BR), is monolithically integrated with an InP-based amplifier circuit to realize a novel opto-electronic integrated circuit (OEIC) which performs as a high-speed photoreceiver. The circuit is realized by epitaxial growth of the field-effect transistors, currently used semiconductor device and circuit fabrication techniques, and selective area BR electro-deposition. The integrated photoreceiver has a responsivity of 175 V/W and linear photoresponse, with a dynamic range of 16 dB, with 594 nm photoexcitation. The dynamics of the photochemical cycle of BR has also been modeled and a proposed equivalent circuit simulates the measured BR photoresponse with good agreement.
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Bacteriorodopsinas/química , Electroquímica/instrumentación , Modelos Químicos , Fotoquímica/instrumentación , Semiconductores , Transductores , Amplificadores Electrónicos , Bacteriorodopsinas/efectos de la radiación , Simulación por Computador , Electroquímica/métodos , Electrónica , Diseño de Equipo , Análisis de Falla de Equipo , Luz , Fotoquímica/métodos , Reproducibilidad de los Resultados , Sensibilidad y Especificidad , Transistores ElectrónicosRESUMEN
This paper summarizes the results found in our laboratory investigating the ultrafast light-induced charge separation in bacteriorhodopsin. A special technique was elaborated for dried oriented samples of long term stability. An upper limit of 21 ps was found by a direct electric method for the early charge separation processes. A permanent electric field on the surface of illuminated samples was demonstrated. The potential application of such samples as ultrafast optoelectric signal transducers is discussed.
Asunto(s)
Bacteriorodopsinas/química , Transducción de Señal , ElectroquímicaRESUMEN
The proposition is that the masterswitch of life and death is invested in the physiological role of the pineal gland which by its neurohormonal influence and its neuronal connections in the brain gears hypothalamic noradrenaline levels to which the function of the autonomic nervous system (ANS) and breathing are integrated. Sudden death in an apparently healthy infant can be compared to a well known event, to what happens to a plant if you close the masterswitch which distributes energy. The plant stops to function. It is not yet realized that the human plant also functions only as long as the masterswitch of its biological organization in the brain in charge of energy substrate availability--BOESA--to which breathing, the need of O2, is also geared, is functioning.
Asunto(s)
Sistema Nervioso Autónomo/fisiología , Glándula Pineal/fisiología , Respiración , Muerte Súbita del Lactante/etiología , Envejecimiento , Sistema Nervioso Autónomo/crecimiento & desarrollo , Homeostasis , Humanos , Lactante , Recién Nacido , Modelos Biológicos , Glándula Pineal/crecimiento & desarrollo , Sueño/fisiologíaRESUMEN
The contention that torpor during hibernation might not be a kind of sleep but a kind of sleep deprivation may be correct, as it can be conceptually explained. Sleep is part of life and cannot be part of the torpor state, which has no reactions. The state of torpor has a time limit and, if this is not observed, death follows by freezing. The evolutional differences particularly in pineal gland physiology may explain the differences between the blueprints governing vital reflexes in humans and hibernators.
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Nivel de Alerta , Hibernación/fisiología , Glándula Pineal/fisiología , Sueño , AnimalesRESUMEN
BACKGROUND: Liver biopsy is the reference standard to assess liver fibrosis in chronic hepatitis C. AIM: To validate and compare the diagnostic performance of non-invasive tests for prediction of liver fibrosis severity and assessed changes in extracellular matrix markers after antiviral treatment. METHODS: The performances of Forns' score, AST to platelet ratio index (APRI), FIB-4 index and Enhanced Liver Fibrosis (ELF) score were validated in 340 patients who underwent antiviral therapy. These scores were determined 24 weeks after treatment in 161 patients. RESULTS: Forns' score, APRI, FIB-4 and ELF score showed comparable diagnostic accuracies for significant fibrosis [area under the receiver operating characteristic curve (AUROC) 0.83, 0.83, 0.85 and 0.81, respectively]. To identify cirrhosis, FIB-4 index showed a significantly better performance over APRI and ELF score (AUROC 0.89 vs. 0.83 and 0.82, respectively). ELF score decreased significantly in patients with sustained virological response (SVR) (P < 0.0001) but remained unchanged in nonresponders. Non-1 hepatitis C virus (HCV) genotype, baseline lower HCV RNA, glucose, hyaluronic acid and higher cholesterol levels were independently associated with SVR. CONCLUSIONS: Simple panel markers and ELF score are accurate at identifying significant fibrosis and cirrhosis in chronic hepatitis C. A decrease in ELF score after antiviral treatment reflects the impact of viral clearance in hepatic extracellular matrix and probably in the improvement of liver fibrosis.
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Hepatitis C Crónica/tratamiento farmacológico , Interferón-alfa/uso terapéutico , Cirrosis Hepática/tratamiento farmacológico , Hígado/patología , Ribavirina/uso terapéutico , Adolescente , Adulto , Anciano , Anciano de 80 o más Años , Antivirales/uso terapéutico , Biomarcadores/sangre , Métodos Epidemiológicos , Femenino , Hepatitis C Crónica/sangre , Humanos , Interferón alfa-2 , Cirrosis Hepática/sangre , Masculino , Persona de Mediana Edad , Recuento de Plaquetas , Proteínas Recombinantes , Adulto JovenRESUMEN
BACKGROUND AND AIMS: Anandamide is an endocannabinoid that evokes hypotension by interaction with peripheral cannabinoid CB1 receptors and with the perivascular transient receptor potential vanilloid type 1 protein (TRPV1). As anandamide has been implicated in the vasodilated state in advanced cirrhosis, the study investigated whether the mesenteric bed from cirrhotic rats has an altered and selective vasodilator response to anandamide. METHODS: We assessed vascular sensitivity to anandamide, mRNA and protein expression of cannabinoid CB1 receptor and TRPV1 receptor, and the topographical distribution of cannabinoid CB1 receptors in resistance mesenteric arteries of cirrhotic and control rats. RESULTS: Mesenteric vessels of cirrhotic animals displayed greater sensitivity to anandamide than control vessels. This vasodilator response was reverted by CB1 or TRPV1 receptor blockade, but not after endothelium denudation or nitric oxide inhibition. Anandamide had no effect on distal femoral arteries. CB1 and TRPV1 receptor protein was higher in cirrhotic than in control vessels. Neither CB1 mRNA nor protein was detected in femoral arteries. Immunochemistry showed that CB1 receptors were mainly in the adventitia and in the endothelial monolayer, with higher expression observed in vessels of cirrhotic rats than in controls. CONCLUSIONS: These results indicate that anandamide is a selective splanchnic vasodilator in cirrhosis which predominantly acts via interaction with two different types of receptors, CB1 and TRPV1 receptors, which are mainly located in perivascular sensory nerve terminals of the mesenteric resistance arteries of these animals.
Asunto(s)
Ácidos Araquidónicos/farmacología , Bloqueadores de los Canales de Calcio/farmacología , Cirrosis Hepática Experimental/fisiopatología , Arterias Mesentéricas/efectos de los fármacos , Vasodilatación/efectos de los fármacos , Animales , Relación Dosis-Respuesta a Droga , Endocannabinoides , Expresión Génica , Canales Iónicos/genética , Canales Iónicos/fisiología , Cirrosis Hepática Experimental/metabolismo , Masculino , Arterias Mesentéricas/fisiopatología , Alcamidas Poliinsaturadas , ARN Mensajero/genética , Ratas , Ratas Wistar , Receptor Cannabinoide CB1/genética , Receptor Cannabinoide CB1/fisiología , Canales Catiónicos TRPVRESUMEN
The electric response signal of dark adapted bacteriorhodopsin excited by a laser flash was studied. By a subtraction method the electric signal corresponding to the bacteriorhodopsin containing 13-cis retinal could be separated. This electric signal contains three components, corresponding to three steps in the charge movement induced by the laser flash. The lifetimes of these steps are correlated with the photocycle of the bacteriorhodopsin containing 13-cis retinal.
Asunto(s)
Bacteriorodopsinas/fisiología , Luz , Retinaldehído/fisiología , Retinoides/fisiología , Adaptación Biológica , Electricidad , Halobacterium , Rayos LáserRESUMEN
In dried oriented samples of purple membrane isolated from Halobacterium halobium, the photoelectric activity decreases and the light adaptation vanishes when the water content of the sample is lowered. In the photocycle the first steps of the proton movement were accelerated with decreasing humidity, while the last steps of the photocycle could not be observed. From the analysis of the photoelectric signal we conclude that at low humidities the protons move forward in the L decay and return to their original place during M decay.
Asunto(s)
Bacteriorodopsinas , Carotenoides , Halobacterium/fisiología , Electrofisiología , Halobacterium/ultraestructura , Potenciales de la Membrana , Fotoquímica , Protones , AguaRESUMEN
In dried oriented samples of purple membranes isolated from Halobacterium halobium the Arrhenius parameters of the photocycle showed an abrupt change at a water content of approximately 80 H2O molecules per bacteriorhodopsin molecule. This makes probable the existence of a water-dependent conformational change of the protein. This result underlines the importance of water in the proton-conduction mechanism inside the protein. The effect of the external electric potential on the rate constants of the photoelectric signals was also measured. The data demonstrate that the membrane potential affects the steps of the proton transport during the photocycle.
Asunto(s)
Bacteriorodopsinas , Carotenoides , Electroquímica , Halobacterium/metabolismo , Fotoquímica , TermodinámicaRESUMEN
Dielectric dispersion effects were studied in purple membranes of different hydration levels. The capacitance and conductivity were measured over the frequency range of 10(2) Hz to 10(5) Hz. With increase in the hydration level, the conductivity increases sharply above the critical hydration of hc = 0.06 g H2O/g protein. This critical hydration is close to the extent of the first continuous strongly bound water layer and is interpreted as the threshold for percolative proton transfer. The capacitance increases continuously with increasing hydration and a larger increase above the water content of 0.1 g H2O/g protein can be seen only at low frequencies. Maxwell-Wagner relaxation also appears above this hydration, showing the presence of a bulk water phase.
Asunto(s)
Bacteriorodopsinas/metabolismo , Conductividad Eléctrica , Agua/metabolismo , Equilibrio HidroelectrolíticoRESUMEN
Fourier transform infrared difference spectroscopy has been used to study the effect of water on the conformation of bacteriorhodopsin. The infrared spectra as a function of water content show a conformational change at about 0.06 g H2O/g bacteriorhodopsin. By an interference method the thickness of the sample was measured and shows similar behavior as a function of water content. This study gives insight into the process of water absorption by purple membrane. The observations are in good agreement with those found for other proteins.