[Assessment of efficacy of allergen immunotherapy in children and adults with allergic rhinitis]. / Otsenka effektivnosti allergen-spetsificheskoi immunoterapii u detei i vzroslykh s allergicheskim rinitom.

The goal of the work toassessment of efficacy and safety of allergen immunotherapy in children and adults with allergic rhinitis, and to conduct a comparative assessment of the effectiveness of subcutaneous (SCIT) and sublingual variant (SLIT) immunotherapy. We have analyzed the medical documentation of patients during the application of allergen immunotherapy for 5 years in 710 patients with allergic rhinitis (AR). According to the results of the validated scale analysis, the clinical efficacy parameters of SCIT and SLIT after a year, 2, 3, and also after 4 and 5 years of therapy were comparable in both children and adults. At the same time, both SCIT and SLIT showed comparable efficacy in both adults and children. Systemic side effects were observed in 10% of children on SCIT and in 7% of children on SLIT. The frequency of systemic reactions in adults was 6% for SCIT, for SLIT it did not exceed 2%.

Electrophysiological correlations of morphological restructuring in experimental local ischemia of different severity in the rat sensorimotor cortex.

Local cerebrovascular disorders were modeled by reversible photochemical clotting of hemispheric cortical vessels. Mild ischemia led to reversible edema in the surface layers of the cortex: cytotoxic edema of the neuropile, primarily of the distal dendrites. This status led to an increase in the lower delta rhythm frequency band power. After administration of systemic anesthetic, delta rhythm appeared sooner in the ischemic foci than in intact cortical areas. More severe ischemia led to the appearance of dark and pyknotic neurons and reduction of oscillation power in all EEG spectrum bands. Restructuring of primarily dendrites caused by local moderate ischemia of the surface cortical layers at the early stage of neurodegenerative processes stimulated the inhibitory recovery processes.

Structural changes in dendritic loci and protective effect of neurotrophic factors from the cerebral cortex of animals with favorable outcome of experimental hemorrhagic stroke.

Structural changes in the sensorimotor cortex of the cerebral hemispheres were studied in rats with experimental hemorrhagic stroke in the internal capsule. Changes in the shape and decrease in the density distribution of spines on apical dendrites of layer V pyramidal neurons of the sensorimotor cortex were revealed. Some fractions isolated from the complex of neurotrophic factors of the cerebral cortex of animals with a favorable outcome of hemorrhagic stroke were shown to affect the distal and proximal dendritic loci. As differentiated from the "nonactive" fraction, the "active" fraction improved the behavior of rats surviving hemorrhagic stroke. The density distribution of spines (particularly in the distal region of apical dendrites of pyramidal neurons in layer V of the cerebral cortex) was stabilized after treatment with the "active" fraction. The "nonactive" fraction had a selective effect on the proximal loci.

[Influence of carbon dioxide on the impregnation of the nervous tissue by silver].

It has been shown that 4% carbon dioxide (CO2) in the air above reaction mixture inhibits the initiation of the formation of silver nanoparticles from complexes with biogenic amines (noradrenaline and serotonin). At the same concentration of CO2 in the air above solution of AgNO3, which is used for staining nerve tissues by the method of Golgi, neurons are preferentially stained, whereas at a concentration of 0.06%, vessels and poor neurons are stained. It is suggested that the entry of free silver ions to neurons is due to the inhibition of sites of initiation of silver nanoparticles in vessels at high CO2 concentrations, while the lack of inhibition leads to silver precipitation in vessels at low CO2 concentrations. It can be assumed that, for stable silver impregnation, the concentration of CO2 must be controlled.

[Isolation of highly purified cyclodextrin glucanotransferase from Bacillus sp. 1070]. / Poluchenie vysokoochishennoi tsiklodekstringliukantransferazy iz Bacillus sp. 1070]

Two chromatographic processes for purification of cyclodextringlucanotransferase (CGTase) from Bacillus sp. 1070 was carried out. The enzyme has been purified into 9.5 times on Butyl-Toyopearl and followed immobilized metal ion chromatography on Cu(II)-Iminodiacetic (IDA)-agarose. By the application of second purification scheme (chromatography on Butyl-Toyopearl and DEAE-Sephacel) the specific activity of CGTase has folded into 13.5 times. The purity of enzyme was shown to be approximately 90% by SDS-electrophoreses data. It was shown that isolated enzyme has two isoelectric points estimated as 5.1 and 5.3.