Use of oligonucleotides conjugated to gold nanoparticles and streptavidin for amplification of optical biosensor signal during detection of telomeric repeats.

Hybridization of telomeric repeats with a complementary oligonucleotide probe was studied by the surface plasmon resonance method. Conjugation of the probe with streptavidin and gold nanoparticles was shown to amplify the signal at similar concentrations of this probe (by 60 and 300 times, respectively). Nanoparticles can be used for biosensor signal amplification in studying the telomerase activity of malignant cells.

[Identification of an alien chromosome in the common wheat line multi 6R].

The chromosome of Agropyron intermedium (Host) Beauv. substituting chromosome 6D has been identified in the karyotype of the Triticum aestivum L. line Multi 6R with the use of C-banding. The alien chromosome, temporarily designated 6Ag1, contains gene(s) of the resistance to the Saratov population of Puccinia recondita Rob. ex. Desm. f. tritici. It has been demonstrated that the resistance gene(s) is(are) highly efficient and that chromosome 6Ag1 is preferably transferred through gametes.

Genetic control for resistance to leaf rust in wheat-Agropyron lines: Agro 139 and Agro 58.

Leaf rust resistance lines of Triticum aestivum carry highly effective Lr genes from Agropyron intermedium (Host) Beauv. This Agro 58 and Agro 139 resistance segregated independently of Agropyron leaf-rust resistance genes Lr-19, Lr-24 and Lr-9 from Ae. umbellulata. Monosomic analysis showed that the Lr gene in Agro 139 was incorporated into wheat chromosome 6D. C-banding analysis could not determine the C-banding pattern of A. intermedium in wheat -Agropyron lines Agro 58 and Agro 139. It is assumed that the transfers occurred from the euchromatin regions of the Agropyron chromosomes to the euchromatin regions of the wheat chromosomes. It is suggested that the Lr gene from Agro 139 be designated LrAg (i)-1 and the Lr gene from Agro 58 designated LrAg (i)-2.