Using comparative genomics to develop a molecular diagnostic for the identification of an emerging pest Drosophila suzukii.

Drosophila suzukii (Spotted Wing Drosophila) has recently become a serious invasive pest of fruit crops in the USA, Canada, and Europe, leading to substantial economic losses. D. suzukii is a direct pest, ovipositing directly into ripe or ripening fruits; in contrast, other Drosophilids utilize decaying or blemished fruits and are nuisance pests at worst. Immature stages of D. suzukii are difficult to differentiate from other Drosophilids, posing problems for research and for meeting quarantine restrictions designed to prevent the spread of this pest in fruit exports. Here we used a combined phylogenetic and bioinformatic approach to discover genetic markers suitable for a species diagnostic protocol of this agricultural pest. We describe a molecular diagnostic for rapid identification of single D. suzukii larva using multiplex polymerase chain reaction. Our molecular diagnostic was validated using nine different species of Drosophila for specificity and 19 populations of D. suzukii from different geographical regions to ensure utility within species.

Drosophila suzukii (Diptera: Drosophilidae) and its Potential Impact to Wine Grapes During Harvest in Two Cool Climate Wine Grape Production Regions.

Drosophila suzukii (Matsumura) is a global pest attacking various berry crops. D. suzukii lays eggs in damaged and in intact wine grape berries of the most soft-skinned varieties. Here, we describe the relative host utilization of different wine grape cultivars grown in Northern Italy and Oregon. Assessments of host berry utilization were performed in both field and laboratory settings. Results were correlated to physiological changes occurring during grape berry development starting at véraison and concluding during harvest. We found that oviposition increased with an increase in sugar content and a decrease of acidity levels. Oviposition increased with a decrease of penetration force. Penetration force, as a measure of skin hardness, is a critical component of host selection among the D. suzukii-exposed cultivars. We demonstrated that incised berries are more favorable for D. suzukii oviposition and as a nutrient substrate. Increased presence on wine grapes, as indicated by egg laying and increased longevity, was observed for flies that were exposed to incised berries as opposed to fully intact berries. D. suzukii flies can be found feeding on damaged wine grapes during the harvest period, especially when the skins of berries are negatively impacted due to cracking, disease, hail injury, and bird damage. Such an increase of feeding and oviposition may increase the likelihood of spoilage bacteria vectoring due to D. suzukii.

The Effects of Kernel Feeding by Halyomorpha halys (Hemiptera: Pentatomidae) on Commercial Hazelnuts.

Halyomorpha halys Stål, the brown marmorated stink bug (Hemiptera: Pentatomidae), is an invasive pest with established populations in Oregon. The generalist feeding habits of H. halys suggest it has the potential to be a pest of many specialty crops grown in Oregon, including hazelnuts, Corylus avellana L. The objectives of this study were to: 1) characterize the damage to developing hazelnut kernels resulting from feeding by H. halys adults, 2) determine how the timing of feeding during kernel development influences damage to kernels, and 3) determine if hazelnut shell thickness has an effect on feeding frequency on kernels. Adult brown marmorated stink bugs were allowed to feed on developing nuts for 1-wk periods from initial kernel development (spring) until harvest (fall). Developing nuts not exposed to feeding by H. halys served as a control treatment. The degree of damage and diagnostic symptoms corresponded with the hazelnut kernels' physiological development. Our results demonstrated that when H. halys fed on hazelnuts before kernel expansion, development of the kernels could cease, resulting in empty shells. When stink bugs fed during kernel expansion, kernels appeared malformed. When stink bugs fed on mature nuts the kernels exhibited corky, necrotic areas. Although significant differences in shell thickness were observed among the cultivars, no significant differences occurred in the proportions of damaged kernels based on field tests and laboratory choice tests. The results of these studies demonstrated that commercial hazelnuts are susceptible to damage caused by the feeding of H. halys throughout the entire period of kernel development.

Gumming Up The Works: Field Tests of a New Food-Grade Gum as Behavioral Disruptor for Drosophila suzukii (Diptera: Drosophilidae).

Drosophila suzukii Matsumura is an economically important pest of small and stone fruits. Its establishment in the Americas and Europe marked an important turning point in crop management programs. Ten years after its first detection, an effective integrated pest management program has yet to be developed and pesticides are mainly used to control this pest. Here we test a new behavioral control tool, with the aim to develop an alternative pest control strategy. A food-grade gum matrix, was evaluated under controlled and open field conditions for its ability to attract the pest and protect the ripening fruit. Here, we report that the gum effectively reduces fruit infestation when used under managed conditions. We show that a single point source can affect D. suzukii behavior over a 3.6 m radius and last for up to 21 d. Open field data reveal that the efficacy of the gum is significantly impacted by water content. We discuss these results in respect to the future implications for D. suzukii management, along with important considerations on gum mechanism of action, possible application strategies and economic suitability for growers.

Effects of powdery mildew fungicide programs on twospotted spider mite (Acari: Tetranychidae), hop aphid (Hemiptera: Aphididae), and their natural enemies in hop yards.

Twospotted spider mite, Tetranychus urticae Koch (Acari: Tetranychidae), and hop aphid, Phorodon humuli (Schrank) (Hemiptera: Aphididae), are the most important arthropod pests of hop (Humulus lupulus L.) in the Northern Hemisphere. A potential barrier for greater adoption of conservation biological control strategies for spider mites and hop aphid is the extensive use of fungicides for management of hop powdery mildew, Podosphaera macularis (Wallr.:Fr.) U. Braun & S. Takamatsu. Field studies conducted in experimental plots in Oregon and Washington in 2005 and 2006 quantified the effects of powdery mildew fungicide programs (i.e., sulfur, paraffinic oil, and synthetic fungicides) on arthropod pests and natural enemies on hop. Fungicide treatment significantly affected spider mite populations in all four studies. Multiple applications of sulfur fungicides applied before burr development resulted in 1.4-3.3-fold greater spider mite populations during summer. Near the cessation of the sulfur applications, or after a lag of 20-30 d, spider mite populations increased significantly faster on sulfur treated plants compared with water-treated plants in three of four experiments. The effect of paraffinic oil on spider mites was varied, leading to exacerbation of spider mites in Oregon and Washington in 2005, suppression of mites in Oregon in 2006, and no significant effect compared with water in Washington in 2006. Significant relative treatment effects for cone damage due to spider mite feeding were detected in Oregon in 2005 in plots treated with sulfur and paraffinic oil compared with water and synthetic fungicides. Mean populations of hop aphids were similar among treatments in Oregon, although sulfur treatment suppressed hop aphid populations in Washington in 2005 and 2006. Populations of individual predacious insect species and cumulative abundance of macropredators were not consistently suppressed or stimulated by treatments in all trials. However, predatory mite abundance in Washington was affected by fungicide treatments, with plots treated with sulfur consistently having 10-fold fewer phytoseiids per leaf compared with the other treatments. Based on the results of these studies, powdery mildew fungicide programs that minimize or eliminate applications of sulfur and paraffinic oil would tend to conserve predatory mites and minimize the severity of spider mite outbreaks. However, mechanisms other than direct or indirect toxicity to phytoseiid mites likely are associated with exacerbation of spider mite outbreaks on hop.

Direct residue analysis of systemic insecticides and some of their relevant metabolites in wines by liquid chromatography - mass spectrometry.

A direct large volume injection (DI-LVI) high performance liquid chromatography - tandem mass spectrometry (HPLC-MS/MS) method was developed and validated for the quantitative determination of 16 systemic insecticides and their main plant metabolites. The assays were conducted on commercial red and white wines made from grapes grown in major wine-producing regions nationally and internationally. Using a 1:20 dilution and an injection volume of 800µL, a limit of quantitation (LOQ) of 1µgL-1 for all analytes was achieved. Matrix-matched standards (MM) were used for accurate quantitation. Imidacloprid (IMI) and methoxyfenozide (MET) were the most frequently detected parent insecticides in the wines reaching concentrations of 1-132µgL-1. Two important plant metabolites imidacloprid-olefin (IMI-OLE) and spirotetramat-enol (SPT-EN) were found at higher concentrations. In five samples SPT-EN was detected in the mgL-1 range with a maximum concentration of 16.3mgL-1 measured in a conventional white wine sample. Most "organic" wines contained no detectable or low insecticide residues, except for one sample, which showed the highest IMI (14.7µgL-1) and IMI-OLE (331µgL-1) concentrations. Considering the maximum residue limit (MRL) definition for the different insecticides, three "conventional" wine samples were non-compliant for SPT. This study highlights the importance to determine both parent and metabolite forms of systemic insecticides in the finished product.

Early diagnosis of spinal epidural metastases using out-patient computed tomographic myelography.

Twenty patients with known malignancies, back pain, abnormal roentgenograms of the spine, and normal neurological examinations were evaluated by outpatient computed tomographic (CT) myelography to determine the presence and extent of epidural tumor. Spinal CT following the intrathecal administration of low doses of water soluble contrast agents provided high quality diagnostic information. Three patients experienced adverse effects from this procedure which were mild and easily managed in the outpatient setting. Epidural tumor was identified in 15 of 20 (75%) patients. Patients were followed for 9-27 months following myelography. The 14 patients with epidural tumor treated with local radiation experienced pain relief and only one of these patients developed signs or symptoms of recurrent epidural tumor in the treated site. This study documents the high incidence of epidural tumor in selected patients without neurological deficits and the excellent palliative results of non-emergent, carefully planned radiation therapy. It also demonstrates that high resolution CT myelography can be performed safely in an outpatient setting in patients at high risk for epidural tumor. Outpatient myelography facilitates the early diagnosis of epidural tumor and provides needed information on the extent of the tumor for radiation treatment planning while conserving health care resources. For these reasons, outpatient CT myelography should be considered in selected patients with cancer who are at high risk for epidural metastases.

Improvements urban and rural women wish to see in their health care services.

This study asked 2000 women randomly selected from the community to comment on their health services. Three-quarters did so; one-quarter were satisfied with currently available services but 45% specified changes they would like to see. The criticisms fell into three broad groups: (a) hospital services where delays, centralisation and referral paths were criticised; (b) general practice where doctor-patient communication was mentioned; and (c) cost of certain health services. Women who criticised currently available services tended to be younger, better educated with higher socioeconomic status, to be in paid employment and to have more often poor health or a close relative with poor health than the women who made no comment. These findings are seen as constructive comments from a thoughtful and informed group of health consumers.

Variety and views in general practice.

Ninety general practitioners described their practices and gave their views on their work. The differences between urban and rural general practices were found to be greater than those between single practices, partnerships and group practices. Rural doctors were on call more frequently (50% being on call more than 11 nights per month and 54% at least every third weekend), had lower adult fees, were more likely to have hospital appointments (54%) and expressed more concern about their independence than urban doctors. Most of the general practitioners (94.5%) said that their surgeries usually ran over time (44% up to an hour, 5.5% longer), longer consultations and extra patients being seen as the prime causes. The general practitioners particularly enjoyed their involvement with people and the variety in their work (34% and 22% of comments respectively), but felt overworked and found difficulties with particular people (33% and 20% of comments respectively). In their general comments the general practitioners wanted to see an improved image for family doctors (23%) and more support for doctors (17%). Fifteen percent of the comments affirmed the quality of general practice.

Mothers' perceptions of access to medical care for children in Otago.

Three hundred urban and 350 rural mothers were surveyed about their subjective perceptions of the availability of a doctor for their child. A third of rural mothers and a quarter of urban mothers expressed concern about lack of access. Shared parenting, a high socioeconomic status of mother and her partner and further training on leaving school, were associated with a greater satisfaction with existing services. Mothers with chronic psychiatric morbidity were more likely to express concern. Approximately one quarter of mothers expressed satisfaction with services overall. Specific improvements for child health services included reduced costs and waiting times together with an increase in the range of services available.

Which women seek help for their psychiatric problems?

The avenues of help used by adult women with minor psychiatric morbidity were investigated in a random community survey from five Otago electoral rolls. A random subsample (n = 314) was subsequently interviewed in their homes. Information on a wide range of sociodemographic items pertinent to women's social roles was collected. Psychiatric illness was determined using a recognised structured diagnostic interview. Some 7.8% of the original population was found to have depressive, anxiety or phobic disorders at the symptom intensity level likely to be found in a psychiatric outpatient clinic. Of these women, just over one-third only (35%) said that they had sought help for their illness from any source. Most (86%) getting help received it from their general practitioner. However, unlike overseas studies where virtually all persons with psychiatric disorder consult their general practitioners for some reason, 13% reported that they had not visited their general practitioners at all in the preceding six months. Alternative therapies were infrequently sought. There was no evidence that health services were being inappropriately used by women with nonmedical problems. The results show that the majority of women are not getting treatments they require for their psychiatric illnesses. The low level of help seeking was particularly marked for young women. The findings suggest poor community awareness of the symptoms of minor psychiatric morbidity and the associated psychosocial consequences.

Screening for psychiatric morbidity in men and women.

This paper describes the use of the general health questionnaire (GHQ) to screen a random sample of men for psychiatric morbidity. The results are contrasted with those from the earlier Otago Women's Health Survey, an investigation into the sociodemographic determinants of psychiatric morbidity in Otago women. The level of psychiatric morbidity found in the men was equal to that found in the women which is in contrast to most overseas studies where men have been found to have lower levels of psychiatric morbidity to women. Significant differences were found in male and female demographic subgroups. High GHQ scores were found in separated, widowed and divorced men, men in higher socioeconomic status groups and those unemployed. High GHQ scores were found among the women aged 18-34, women who had never married, those in lower socioeconomic status groups and those unemployed. This study illustrates that gender needs to be considered alongside traditional sociodemographic factors when studying psychiatric morbidity and symptomatology.

Developmental parameters and seasonal phenology of Calepitrimerus vitis (Acari: Eriophyidae) in wine grapes of western Oregon.

Developmental parameters of protogyne Calepitrimerus vitis (Nalepa) (Acari: Eriophyidae) were determined at 12, 15, 17, 22, 25, 28, 31, and 34 °C to better understand seasonal activity, population growth, and ultimately more effectively manage pest mites in wine grapes. Net reproductive rate (R(o)) was greater than zero at all temperatures with the maximum R(o) (9.72) at 25 °C. The lowest estimated R(o) (0.001) occurred at 34 °C. There was a gradual decrease in mean generation time (T) as temperatures increased from 17 to 31 °C. The shortest and longest generation time was recorded at 31 °C (T = 5.5 d) and 17 °C (T = 17.5 d). Rates of natural increase were lowest at 17°C (0.035) and increased with increasing temperatures, respectively. The peak rate of natural increase value (0.141) was at 25 °C. Estimations for minimum and maximum developmental thresholds were 10.51 and 39.19 °C, respectively, while the optimum developmental temperature was 26.9 °C. The thermal constant for egg to adult development was estimated at 87.7DD. The highest fecundity was observed at 25 °C. These parameters indicated that mites begin feeding at the onset of shoot growth when tissue is most susceptible in spring. Historical weather data showed that vines are in this susceptible growth stage for longer periods in the cool Willamette Valley compared with warmer Umpqua and Applegate/Rogue Valley regions. Estimation of degree-days indicated when deutogyne mites move to overwintering refuge sites. Degree-day accumulations indicated up to 14 generations per growing season.

Impact of sexual and physical abuse on women's mental health.

The level of psychiatric symptomatology was assessed with the General Health Questionnaire and the Present State Examination in a random community sample of women. Subsequently it was ascertained which of the women had been the victims of sexual or physical abuse, in either childhood or adult life. Women with a history of being abused were significantly more likely to have raised scores on both measures of psychopathology and to be identified as psychiatric cases. 20% of women who had been exposed to sexual abuse as a child were identified as having psychiatric disorders, predominantly depressive in type, compared with 6.3% of the non-abused population. Similar increases in psychopathology were found in women who had been physically or sexually assaulted in adult life. These findings indicate that the deleterious effects of abuse can continue to contribute to psychiatric morbidity for many years.

PIP: A study conducted in New Zealand examined the relation between women's mental health and past experiences of sexual and physical abuse in a randomly selected community sample. The level of psychiatric symptomatology was assessed using the General Health Questionnaire (GHQ) and the Present State Examination (PSE). About 13.1% of the women interviewed and 9.9% of the original random sample reported having some form of sexual abuse as a child. About 4.6% of the subjects interviewed and some 3.5% of the original sample reported experiencing sexual abuse as an adult. About 20.1% of the interviewed sample and 16.2% of the original sample identified themselves as having been physically abused as an adult. These women with histories of abuse have higher GHQ and PSE scores than the nonabused and were more likely to be identified as psychiatric cases. Psychiatric disorders were identified in 20% of women who experienced childhood sexual abuse; similar increases in psychopathology were found in women who had been abused as an adult. The results of this study indicate that the harmful effects of abuse can continue to contribute to the psychiatric morbidity of women for many years.

A study of women who refused to participate in a community survey of psychiatric disorder.

The bias resulting from nonresponse causes problems for all epidemiological research. This study investigated the characteristics of those who refused to participate in a community survey of female psychiatric disorder. The demographic characteristics of refusers were similar to those previously reported. When compared to responders, those who refused tended to be older, never married and not widowed, of lower socioeconomic status and urban dwellers. No differences in physical illness requiring hospitalization or inpatient and outpatient psychiatric illness, as assessed through hospital records, were discerned between refusers and responders. Reasons for refusal are discussed. The bias problem may get worse as changing community attitudes to privacy and patients' rights lead to greater refusal rates.

Marriage, motherhood and psychiatric morbidity in New Zealand.

The prevalence of psychiatric disorder, as assessed by both the GHQ-28 and the short PSE, is described for a random community sample of New Zealand women. In contrast to previous studies, married and widowed women and mothers showed lower rates than the never married and childless women. A plausible explanation is provided by available analysis of New Zealand gender roles. Such an explanation would reconfirm the importance of socio-cultural factors in community psychiatric disorder.

Validity of the GHQ-28 in New Zealand women.

The GHQ-28 was validated against the short PSE in a New Zealand community study of female psychiatric morbidity. The GHQ-28 total scores were significantly correlated with the PSE scores. Higher coefficients were obtained using the scoring method of Goodchild and Duncan-Jones than with the standard scoring method. In this data set, the 3/4 cutoff had the best sensitivity and specificity. The correlations of the GHQ-28 subscales with ICD diagnostic classes and ad hoc PSE sub-scores were also statistically significant. Because the distribution of the GHQ-28 scores is positively skewed, non-parametric statistics may be preferable to the traditional Pearson's correlation coefficient. Overall, the results from this study confirm the GHQ-28 to be a valid and practical screen for presence or absence of psychiatric disorder in New Zealand women.

Alcohol-related problems in New Zealand women.

As total alcohol consumption has increased this half century in most developed countries, alcohol-related problems have become more frequent. Most research has either studied only men or failed to mention gender. This study examined the prevalence of alcohol problems and their socio-demographic associations in a random sample of New Zealand women. Women of younger age, who were unmarried, well educated, in employment, with child care support and who lived in rural communities saw themselves as having more problems with alcohol. Women who had experienced physical or sexual abuse as adults had increased rates of alcohol problems as did those with more psychiatric morbidity as assessed by the General Health Questionnaire and the short Present State Examination. However, women with multiple social roles, particularly caring responsibilities, were less likely than women with one or two social roles to view themselves as having alcohol problems. The data provided no support for the role strain hypothesis of alcohol abuse. It is argued that the findings support a social explanation for alcohol problems based on varying social sanctions on drinking and alcohol availability rather than a psychoanalytic one of unconscious conflicts over femininity, sexuality or female social roles.

Otago Women's Health Survey 30-month follow-up. I: Onset patterns of non-psychotic psychiatric disorder.

Women who showed new psychiatric morbidity after a 30-month follow-up of a random community sample of New Zealand women were compared with those who were free of psychiatric disorder at follow-up. There were 25 new cases among the 215 women who were originally not psychiatric cases and who were re-interviewed. Using the weighted back population data to obtain prevalence figures for the general population, 6.9% became new cases over the two and a half years. Those who developed psychiatric disorder initially showed more psychiatric symptoms at a subdiagnostic level. Baseline factors that preceded the onset of psychiatric disorder were being separated or divorced, coming from a large family, having poor social networks, living alone, having few social role responsibilities such as paid employment or motherhood, and having poor physical health. An additional cross-sectional association at follow-up was poor financial security. Good social networks were closely linked with the number of a woman's social roles and appeared to protect her against the onset of psychiatric disorder. Consistent with the initial cross-sectional study, the follow-up data provide no support for marriage and child-care being risk factors for female psychiatric disorder. However, in New Zealand, these factors indicate social integration and are associated with superior mental health.