RESUMEN
AIM: To investigate if the nucleoside analogue lamivudine (LAM), a potent inhibitor of HBV replication, could restore the function of dendritic cells derived from patients with chronic hepatitis B (CHB) in an Asian population. METHODS: Dendritic cells (DCs) derived from mononuclearcytes of patients with chronic HBV infection were cultured in the presence of IL-4, granulocyte-macrophage colony-stimulating factors (GM-CSF) and gradient concentrations of LAM (0-2 mmol/L). Cell morphology was observed under light microscopy. Cell surface molecules, including HLA-DR, CD80, CD83, and CD1alpha, were analyzed with flow cytometry. The concentrations of IL-6 and IL-12 in the supernatant were assayed by ELISA. T cell proliferation was assayed by methyl thiazolyl tetrazolium (MTT). RESULTS: The expression of CD1alpha on DC treated with 0.5 mmol/L LAM (LAM-DC 0.5 mmol/L) was significantly higher than that of DC untreated with LAM (54.1 +/- 4.21 vs 33.57 +/- 3.14, P < 0.05), and so was the expression of CD83 (20.24 +/- 2.51 vs 12.83 +/- 2.12, P < 0.05) as well as the expression of HLA-DR (74.5 +/- 5.16 vs 52.8 +/- 2.51, P < 0.05). Compared with control group, LAM-DC group (0.5 mmol/L) secreted significantly more IL-12 (910 +/- 91.5 vs 268 +/- 34.3 pg/mL, P < 0.05), had lower levels of IL-6 in the culture supernatant (28 +/- 2.6 vs 55 +/- 7.36 pg/mL, P < 0.05), markedly enhanced the stimulatory capacity in the allogeneic mixed leukocyte reaction (MLR) (1.87 +/- 0.6 vs 1.24 +/- 0.51, P < 0.05). CONCLUSION: The lower expression of phenotypic molecules and impaired allogeneic mixed lymphocyte reaction function of dendritic cells derived from patients with HBV infection could be restored in vitro by incubation with LAM.
Asunto(s)
Células Dendríticas/efectos de los fármacos , Virus de la Hepatitis B/efectos de los fármacos , Hepatitis B Crónica/tratamiento farmacológico , Lamivudine/farmacología , Inhibidores de la Transcriptasa Inversa/farmacología , Antígenos CD/metabolismo , Asia , Forma de la Célula/efectos de los fármacos , Células Cultivadas , Células Dendríticas/inmunología , Células Dendríticas/patología , Células Dendríticas/virología , Relación Dosis-Respuesta a Droga , Antígenos e de la Hepatitis B/sangre , Virus de la Hepatitis B/crecimiento & desarrollo , Hepatitis B Crónica/inmunología , Hepatitis B Crónica/patología , Humanos , Inmunofenotipificación , Interleucina-12/metabolismo , Interleucina-6/metabolismo , Lamivudine/uso terapéutico , Activación de Linfocitos , Prueba de Cultivo Mixto de Linfocitos , Inhibidores de la Transcriptasa Inversa/uso terapéutico , Linfocitos T/inmunología , Factores de Tiempo , Replicación Viral/efectos de los fármacosRESUMEN
PURPOSE: To investigate the expression of αB-crystallin and its possible role of anti-apoptosis in oral verrucous carcinoma. METHODS: The expression of αB-crystallin and activated caspase-3 was detected in oral verrucous carcinoma, oral squamous carcinoma and normal mucosa by immunohistochemistry, and their relationship was investigated. SPSS 16.0 software package was used for statistical analysis. Nonparametric test and spearman correlation test were performed. RESULTS: The expression of αB-crystallin in oral verrucous carcinoma and oral squamous carcinoma was significantly higher than that in normal mucosa(P<0.05). And in oral verrucous carcinoma, the increase of expression of αB-crystallin coincided with the decrease of expression of activated caspase-3(P<0.05). CONCLUSION: αB-crystallin may play a role of anti-apoptosis by inhibiting the activation of caspase-3 in oral verrucous carcinoma.