Behavioral intention to use distance teaching in the pandemic era.

This study aimed at exploring the impact of post-epidemic era on teachers' behavioral intention of distance education. In this study, purposive sampling method was used to enroll 390 teachers in colleges and universities, high schools and vocational schools, and junior high and elementary schools to be the research subjects for the questionnaire survey. A total of 360 questionnaires were collected for statistics, and AMOS 23.0 statistical software was used to analyze the correlation between variables. Meanwhile, a structural equation model was used to analyze and verify the impact of the technology acceptance model, information system success model, and diffusion of innovations theory. According to the results, the system quality of distance teaching platforms certainly affected perceived usefulness, and perceived usefulness affected teachers' attitudes toward using distance teaching systems. In addition, perceived ease of use had an impact on perceived usefulness, and trialability had an impact on teachers' attitudes toward using distance teaching systems. Finally, teachers' attitude toward using a distance teaching system also affected their behavioral intention. According to the results, suggestions were made in this study to teachers and students for distance teaching. Additionally, the main contribution of this study lies in providing specific strategies for further distance teaching through empirical results in combination with on-site teaching.

The impact of online teaching in behavior intention for college students in Taiwan.

This paper aims to explore the change of learning mode of college students from physical courses to online courses due to the impact of the COVID-19 pandemic. The questionnaire survey method is used to conduct research on the behavior intentions of college students in online teaching under the pandemic. A total of 600 questionnaires are distributed, and 530 questionnaires are collected, for a recovery rate of 88%. A total of 493 supported questionnaires are received, for an effective recovery rate of 93%. Descriptive statistics of data analysis are used to analyze the distribution of background variables of college students, and a structural equation model is used to analyze and verify the impact of external variables (trust, convenience, perceived critical mass) on the technology acceptance model (perceived usefulness, perceived ease of use, attitude, and behavior intention). The results found no significant impact of the trust of college students in online teaching on the perceived usefulness, and significant impact of trust on the perceived ease of use. There is significant impact of convenience on perceived usefulness, and no significant impact of convenience on perceived ease of use. There is no significant impact of perceived critical mass on perceived usefulness, and significant impact of perceived critical mass on perceived ease of use. There is significant impact of perceived ease of use on perceived usefulness, and significant impact of perceived usefulness on attitude. There is significant impact of perceived ease of use on attitude, and significant impact of attitude on behavior intention. Based on the research results, practical suggestions and research suggestions are proposed in this research, which can be used as a reference for college students to use online courses for learning.

A hybrid-membrane migration method to isolate high-purity adipose-derived stem cells from fat tissues.

Human adipose-derived stem cells (hADSCs) exhibit heterogeneous characteristics, indicating various genotypes and differentiation abilities. The isolated hADSCs can possess different purity levels and divergent properties depending on the purification methods used. We developed a hybrid-membrane migration method that purifies hADSCs from a fat tissue solution with extremely high purity and pluripotency. A primary fat-tissue solution was permeated through the porous membranes with a pore size from 8 to 25 µm, and the membranes were incubated in cell culture medium for 15-18 days. The hADSCs that migrated from the membranes contained an extremely high percentage (e.g., >98%) of cells positive for mesenchymal stem cell markers and showed almost one order of magnitude higher expression of some pluripotency genes (Oct4, Sox2, Klf4 and Nanog) compared with cells isolated using the conventional culture method.

Purification of human adipose-derived stem cells from fat tissues using PLGA/silk screen hybrid membranes.

The purification of human adipose-derived stem cells (hADSCs) from human adipose tissue cells (stromal vascular fraction) was investigated using membrane filtration through poly(lactide-co-glycolic acid)/silk screen hybrid membranes. Membrane filtration methods are attractive in regenerative medicine because they reduce the time required to purify hADSCs (i.e., less than 30 min) compared with conventional culture methods, which require 5-12 days. hADSCs expressing the mesenchymal stem cell markers CD44, CD73, and CD90 were concentrated in the permeation solution from the hybrid membranes. Expression of the surface markers CD44, CD73, and CD99 on the cells in the permeation solution from the hybrid membranes, which were obtained using 18 mL of feed solution containing 50 × 104 cells, was statistically significantly higher than that of the primary adipose tissue cells, indicating that the hADSCs can be purified in the permeation solution by the membrane filtration method. Cells expressing the stem cell-associated marker CD34 could be successfully isolated in the permeation solution, whereas CD34⁺ cells could not be purified by the conventional culture method. The hADSCs in the permeation solution demonstrated a superior capacity for osteogenic differentiation based on their alkali phosphatase activity, their osterix gene expression, and the results of mineralization analysis by Alizarin Red S and von Kossa staining compared with the cells from the suspension of human adipose tissue. These results suggest that the hADSCs capable of osteogenic differentiation preferentially permeate through the hybrid membranes.