Establishment and identification of the gill cell line from the blunt snout bream (Megalobrama amblycephala) and its application in studying gill remodeling under hypoxia.

To probe the mechanisms of gill remodeling in blunt snout bream under hypoxic conditions, we selected gill tissue for primary cell culture to establish and characterize the first blunt snout bream gill cell line, named MAG. The gill cells were efficiently passaged in M199 medium supplemented with 8% antibiotics and 15% fetal bovine serum at 28 °C, exhibiting primarily an epithelial-fibroblast mixed type. Additionally, the MAG cells (17th generation) were subjected to four experimental conditions-normoxia, hypoxia 12 h, hypoxia 24 h, and reoxygenation 24 h (R24h)-to evaluate the effects of hypoxia and reoxygenation on MAG cells during gill remodeling. We found that the MAG cell morphology underwent shrinkage and mitochondrial potential gradually lost, even leading to gradual apoptosis with increasing hypoxia duration and increased reactive oxygen species (ROS) activity. Upon reoxygenation, MAG cells gradually regain cellular homeostasis, accompanied by a decrease in ROS activity. Analysis of superoxide dismutase (SOD), glutathione (GSH), lactate dehydrogenase (LDH), catalase (CAT), anti-superoxide anion, and other enzyme activities revealed enhanced antioxidant enzyme activity in MAG cells during hypoxia, aiding in adapting to hypoxic stress and preserving cell morphology. After reoxygenation, the cells gradually returned to normoxic levels. Our findings underscore the MAG cells can be used to study hypoxic cell apoptosis during gill remodeling. Therefore, the MAG cell line will serve as a vital in vitro model for exploring gill remodeling in blunt snout bream under hypoxia.

Kinetic and mechanistic investigation of geosmin and 2-methylisoborneol degradation using UV-assisted photoelectrochemical.

The taste and odor (T&O) problem represented by 2-methylisoborneol (2-MIB) and geosmin (GSM) in water is the multiple undesirable substances in the drinking water and the aquatic industry. In this study, the UV-assisted photoelectrochemical, a prospective advanced oxidation process (AOP), was evaluated for the degradation of 2-MIB and GSM. In contrast to UV photochemical and electrochemical, the degradation ratio of GSM (2-MIB) increase to 96% (95%) in 25 min. The removal ratio and rate depended on reaction time, electrolyte concentration, current density, and water quality parameters (e.g. pH, HCO3-, natural organic matter, and tap water). Among these parameters, a high concentration of electrolyte and acidic solutions could accelerate the rate and increase the ratio, while alkaline conditions and the impurity content had negative effects. Furthermore, the significant role of various reactive species (e.g. HO∙, Cl, ClO, etc) were highlighted by scavenging experiments. Complex free radicals exist was further verified by electron paramagnetic resonance spectroscopy (EPR) experiments. The intermediates were identified and the possible degradation pathways during the UV-assisted photoelectrochemical reactions of both compounds were proposed. Overall, the UV-assisted photoelectrochemical is beneficial to the removal of GSM and 2-MIB in water.