Summary of the best evidence for sedation monitoring in children / 中国实用护理杂志

Objective:To search, evaluate and integrate the available evidence on pediatric sedation monitoring, and summarize the best evidence.Methods:Up To Date, BMJ Best Practice, Canadian Medical Association Clinical Practice Guidelines (CMA Infobase), Registered Nurses′ Association of Ontario (RNAO), National institute for Health and Care Excellence (NICE), Evidence-Based Medicine, Cochrane Library, Yimaitong, PubMed, Embase, Wanfang Database, CNKI were searched to collect evidence related to the monitoring of pediatric sedation, including clinical decision-making, guidelines, expert consensus, and evidence-related original research. The search time limit was from the establishment of the database to August 2020. The quality of the literature was evaluated by the suitable evaluated tool based on their types. The level and recommedation grade of the evidence were appraised by the suitable tools of JBI.Results:A total of 13 articles were finally included. Twenty best evidences were summarized, including three aspects of monitoring content, monitoring timing, and monitoring tools.Conclusions:The research summarized the best evidence for pediatric sedation monitoring, and provided evidence-based references for clinical practice and standard formulation. Most of the evidence was form foreign resource. It is suggested that clinical departments or institutions should fully consider the clinical situation. At the same time, high-quality randomized controlled trials should be conducted one step further.

Correlations Between Single Nucleotide Polymorphisms, Cognitive Dysfunction, and Postmortem Brain Pathology in Alzheimer's Disease Among Han Chinese / 神经科学通报·英文版

In this study, the distribution of five Alzheimer's disease (AD)-related single nucleotide polymorphisms (SNPs) in the Han population was examined in combination with the evaluation of clinical cognition and brain pathological analysis. The associations among SNPs, clinical daily cognitive states, and postmortem neuropathological changes were analyzed in 110 human brains from the Chinese Academy of Medical Sciences/Peking Union Medical College (CAMS/PUMC) Human Brain Bank. APOE ε4 (OR = 4.482, P = 0.004), the RS2305421 GG genotype (adjusted OR = 4.397, P = 0.015), and the RS10498633 GT genotype (adjusted OR = 2.375, P = 0.028) were associated with a higher score on the ABC (Aβ plaque score, Braak NFT stage, and CERAD neuritic plaque score) dementia scale. These results advance our understanding of the pathogenesis of AD, the relationship between pathological diagnosis and clinical diagnosis, and the SNPs in the Han population for future research.

Identification of a HPGD mutation in three families affected with primary hypertrophic osteoarthropathy / 中华医学遗传学杂志

<p><b>OBJECTIVE</b>To detect mutation of HPGD gene among three pedigrees affected with primary hypertrophic osteoarthropathy (PHO) by DNA sequencing and high-resolution melting (HRM) analysis.</p><p><b>METHODS</b>Genomic DNA was extracted from peripheral blood samples collected from the pedigrees. PCR and direct sequencing were carried out to identify potential mutations of the HPGD gene. Amplicons containing the mutation spot were generated by nested PCR. The products were then subjected to HRM analysis using the HR-1 instrument. Direct sequencing was carried out in family members and healthy individuals to confirm the result of HRM analysis.</p><p><b>RESULTS</b>A homozygous mutation c.310_311delCT was detected in 2 affected probands, while a heterozygous mutation c.310_311delCT was detected in the third proband. HRM analysis of the fragments encompassing HPGD exon 3 showed 3 curve patterns representing three different genotypes, i.e., the wild type, the c.310_311delCT homozygote, and the c.310_311delCT heterozygote. Result of DNA sequencing was consistent with that of the HRM analysis and phenotype of the subjects.</p><p><b>CONCLUSION</b>The c.310_311delCT mutation may be the most prevalent mutation among Chinese population. HRM analysis has provided an optimized method for genetic testing of HPGD mutation for its simplicity, rapid turnover and high sensitivity.</p>

Efficacy of Brief Behavioral Therapy for Insomnia （BBT-I） in patients suffering Paradoxical insomnia / 中风与神经疾病杂志

@#To explore the effect of BBT-I on subjective insomnia and its influencing factors. Methods 60 primary insomnia patients were collected in outpatient department，and divided into two groups：Subjective insomnia group （SI group） and non-subjective insomnia group （NSI group） by sleep data collected by standard polysomnography monitoring and Pittsburgh Sleep Quality Index （PSQI），sleep diaries during the intervention were collected，and the Pre Sleep Arousal Scale（PSAS），Brief Version of Dysfunctional Beliefs and Attitudes About Sleep （DBAS） and the Sleep Hygiene Practice Scale（SHPS） were collected to evaluate the influencing factors related to sleep attitudes，beliefs and behaviors of patients. All patients received BBT-I for 4 weeks，and collected the sleep diaries and PSQI 1 week before and 1 week after treatment. Results Compared with NSI group，SI group had higher arousal index（P<0.05）；compared with NSI group，SI group had lower scores in factor 3 indicated as drug belief and factor 4 representing anticipation of sleep in DBAS，that is，there were more irrational beliefs in SI （P<0.05）.Intragroup comparison showed that there were statistically significant differences insleep latency、sleep efficiency，daytime function and PSQI score in SI group before and after treatment （P<0.05），and there were statistically significant differences in SE difference before and after intervention between SI group and NSI group （P<0.05）.In the subjective insomnia group，daytime functional difference before and after intervention was negatively correlated with the first factor in DBAS（r=0.270，P=0.037）. daytime functional difference before and after intervention was negatively correlated with PSAS（r=0.268，P=0.039）；The difference of sleep latency before and after intervention was negatively correlated with physiological stimulation of PSAS（r =0.288，P=0.042）. Conclusion Compared with nonsubjective patients，subjective insomnia patients have higher arousals and more irrational beliefs and attitudes.BBT-I may improve sleep latency/、sleep efficiency，daytime function and sleep sensation in subjective insomnia patients.