RESUMEN
DNA synthesis and mitosis in frog lens epithelium are abolished by hypophysectomy and restored by somatomedin C. Both growth hormone and triiodothyronine also restore lens cell proliferation in vivo but not in vitro. Somatomedin-like activity in frog serum is diminished by hypophysectomy and is restored by growth hormone and triiodothyronine.
Asunto(s)
Ciclo Celular/efectos de los fármacos , Hipofisectomía , Cristalino/citología , Somatomedinas/farmacología , Animales , Anuros , Replicación del ADN/efectos de los fármacos , Células Epiteliales , Hormona del Crecimiento/farmacología , Factor I del Crecimiento Similar a la Insulina , Interfase/efectos de los fármacos , Metamorfosis Biológica , Mitosis/efectos de los fármacos , Somatomedinas/sangre , Triyodotironina/farmacologíaRESUMEN
We developed an animal model in the rabbit eye with intravitreally injected heterotransplanted bovine retinal pigment epithelial cells to test a pharmacological agent which would reduce extracellular matrix formation. Results with 20 and 60 micrograms of cis-hydroxyproline showed a 48% decrease in the rate of traction retinal detachments.
Asunto(s)
Hidroxiprolina/uso terapéutico , Enfermedades de la Retina/tratamiento farmacológico , Cuerpo Vítreo , Animales , Bovinos , Colágeno/metabolismo , Matriz Extracelular/metabolismo , Oftalmopatías/complicaciones , Oftalmopatías/tratamiento farmacológico , Oftalmopatías/metabolismo , Oftalmopatías/patología , Masculino , Procedimientos Quirúrgicos Oftalmológicos , Epitelio Pigmentado Ocular/citología , Epitelio Pigmentado Ocular/trasplante , Conejos , Desprendimiento de Retina/prevención & control , Enfermedades de la Retina/complicaciones , Enfermedades de la Retina/metabolismo , Enfermedades de la Retina/patología , Estereoisomerismo , Cuerpo Vítreo/efectos de los fármacos , Cuerpo Vítreo/ultraestructuraRESUMEN
A model of proliferative vitreoretinopathy (PVR) is described. This is the first reported model based on the transplantation of heterologous retinal pigment epithelium cells into the vitreous cavity of rabbits. Freshly isolated cells or short-term primary cultures of bovine pigment epithelium are employed. Strands develop in the vitreous cavity and, with a frequency now approaching 100%, traction retinal detachments develop by 3-4 weeks. Light and electron microscopic features of this model parallel those found for transplanted autologous RPE cells; there are no overt signs of rejection. Examination of the strands should prove useful in identifying strategies of therapeutic value. This model permits such studies, since retinal detachments in adequate numbers are readily produced. The model resembles human PVR.