RESUMEN
Clinical symptoms and haematological disorders in patients suffering from lymphoplasmatic/lymphoplasmocytoidal lymphoma were presented, 82 patients were examined (median age of 63), all of the in clinical stage IV (ANN Arbor scale). In all of the patients bone marrow was invaded. The most common symptom was enlargement of peripheral lymph nodes (86%) less often abdominal ones (21%). Splenomegalia was found in 55% and hepatomegalia in 49% of patients 35% of patients had anaemia and 38% thrombocytopenia. Close to 1/3 of patients dermatoses were found, in most cases allergic ones. In 7% another cancer was found.
Asunto(s)
Leucemia Linfocítica Crónica de Células B/diagnóstico , Adulto , Anciano , Anciano de 80 o más Años , Anemia/diagnóstico , Femenino , Humanos , Ganglios Linfáticos , Masculino , Persona de Mediana Edad , Estudios Retrospectivos , Trombocitopenia/diagnósticoRESUMEN
Minimal residual disease (MRD) can be detected only by sophisticated methods, e.g. immunologic. The purpose of the study was to detect MRD in leukemia patients (AL) being in hematological and clinical remission (CR) by immunophenotyping of bone marrow cells and to define the role of the immunologic method in the detection of MRD. Bone marrow samples from 31 patients with ALL and AML and from 18 volunteers were taken several times. Staining of the cells was performed by double color immunofluorescence (IF) and double color immunoenzyme assay (APAAP). The detection of MRD was done by the comparison of the percent of positive cells between AL and normal cases. Each pair of the antigens (CD) was estimated in its usefulness in MRD detection. The statistical analyses were evaluated with a t-Student's test.
Asunto(s)
Antígenos CD/análisis , Biomarcadores de Tumor/análisis , Médula Ósea/química , Inmunofenotipificación/métodos , Leucemia-Linfoma Linfoblástico de Células Precursoras/diagnóstico , Adolescente , Adulto , Anciano , Niño , Preescolar , Femenino , Humanos , Masculino , Persona de Mediana Edad , Neoplasia ResidualRESUMEN
The term minimal residual disease (MRD) describes leukemia cells present in bone marrow at a level below that detectable by conventional methodology in patients being in complete hematological and clinical remission (CR). The purpose of the study was, the detection of MRD in leukemia patients (AL-MRD+), the correlation between AL-MRD+ and the chosen hematological and clinical parameters and the follow up, and the prognosis. Bone marrow samples from 31 patients with ALL and AML, and from 18 volunteers were taken several times. Staining of cells was performed by double color immunofluorescence (IF), using flow cytometer and double color immunoenzyme assay (APAAP). The statistical analysis were evaluated with a t-Student's test and chi-squared test.
Asunto(s)
Antígenos CD/análisis , Biomarcadores de Tumor/análisis , Médula Ósea/química , Leucemia Mieloide Aguda/diagnóstico , Leucemia-Linfoma Linfoblástico de Células Precursoras/diagnóstico , Adolescente , Adulto , Anciano , Distribución de Chi-Cuadrado , Niño , Preescolar , Femenino , Humanos , Masculino , Persona de Mediana Edad , Neoplasia Residual , PronósticoAsunto(s)
Arginasa/sangre , Leucemia Linfocítica Crónica de Células B/enzimología , Linfocitos/enzimología , Arginasa/efectos de los fármacos , Activación Enzimática , Humanos , Leucemia Linfocítica Crónica de Células B/tratamiento farmacológico , Linfocitos/efectos de los fármacos , Manganeso/farmacología , Estadificación de NeoplasiasRESUMEN
Among 82 patients with L-LP in clinical stage IV, 14% were found to have monoclonal gammapathy and 22% polyclonal gammapathy. The other classes of immunoglobulins were not affected. In 78%, cell immunity was impaired. Decrease in T cell count (CD2+ and CD3+) in blood was found. Particularly T helper cells (CD4+), to a lesser degree T suppressor cells (CD8+) were affected. The life time of patients varied from 3 to 120 months. 60% of deaths were caused by infection.