Evaluation of flow-cytometric three-parameter analysis for EGFR quantification and DNA assessment in human bladder carcinomas.

Flow-cytometric multi-parameter staining is an excellent method for defining tumour subpopulations. This provides further understanding of tumour heterogeneity and defines the biological relevance of tumour subpopulations. A method of quantifying the epidermal growth factor receptor (EGFR) in parallel with DNA staining, which was previously established in bladder carcinoma cell lines, was applied to twenty-five biopsies of urothelium and urothelial neoplasms. Uro5, a surface glycoprotein, was used to identify urothelial cells. Objective quantification of receptor content via flow cytometry was achieved with beads of defined numbers of antigen-binding sites, and receptor numbers obtained from urothelial and nonurothelial cells were compared with staining intensity in a three-step immunoperoxidase detection of the EGFR. The data obtained matched the immunohistochemical findings and were more sensitive in the low range (ca. 5x103) of receptors. Parallel definition of the proliferative fraction and DNA-ploidy of tumour cells means that this method satisfies the requirements of objective quantification for oncological diagnosis.

Endosteal implants in the irradiated lower jaw.

Since 1990 Endosteal implants have been inserted in the irradiated lower jaw at our clinic. IMZ implants have been used for dental rehabilitation in 26 patients (21 male, 5 female) suffering from squamous cell carcinomas stage T2-T4 136. The implants were either placed in local bone and soft tissue (group 1, n = 60 implants), or in local bone after marginal mandibulectomy and transplanted soft tissue (group 2, n = 26 implants), or in transplanted bone and soft tissue (group 3, n = 52 implants). Life-table analysis according to Kaplan-Meier demonstrated a 3-year implant survival rate of 87.8% in Group 1, 69.1% in Group 2 and 58.3% in Group 3. There was no statistical significant difference in the amount of marginal bone loss and the degree of marginal infection between the three groups (P > 0.29). Major complications: A mandibular fracture passing through an empty implant socket 8 months after implant loss (Group 2) was caused by postradiation-osteonecrosis; implant removal and bone resection was mandatory. The poor results of the bone graft group may be explained by two patients, in whom simultaneous placement of implants in nonvascularized bone grafts was carried out, intraoral tissue breakdown led to graft failure and loss of the implants (n = 10).

MR imaging of the inner ear and cerebellopontine angle: comparison of three-dimensional and two-dimensional sequences.

OBJECTIVE: The aim of the study was to compare the ability of three-dimensional (3D) T2-weighted turbo spin-echo and gadolinium-enhanced 3D T1-weighted gradient-echo sequences with two-dimensional (2D) T2-weighted turbo spin-echo and gadolinium-enhanced T1-weighted spin-echo sequences to reveal anatomic and pathologic structures of the inner ear and cerebellopontine angle. SUBJECTS AND METHODS: Thirty-one patients underwent axial 2D T2-weighted turbo spin-echo and 3D T2-weighted turbo spin-echo MR imaging, axial and coronal 2D T1-weighted spin-echo MR imaging before and after i.v. injection of gadopentetate dimeglumine, and gadolinium-enhanced axial 3D T1-weighted gradient-echo MR imaging. The visualization of anatomic and pathologic structures on the different sequences was evaluated. Statistical analysis was performed from the data obtained from the visual evaluation of the anatomic structures on the different sequences. Signal-to-noise and contrast-to-noise ratios were calculated for the gadolinium-enhanced 3D T1-weighted gradient-echo and 2D T1-weighted spin-echo sequences, and statistical evaluation was performed. RESULTS: The 3D sequences enabled excellent visualization of 94% of all evaluated anatomic structures, and the 2D sequences enabled excellent visualization in only 3% of these structures. Pathologic structures were revealed in all cases by one or both of the 3D sequences. Diagnosis in all patients could be made by using the combination of the 3D T2-weighted turbo spin-echo and the gadolinium-enhanced 3D T1-weighted gradient-echo sequences. However, the 2D sequences failed to show pathologic structures in three patients. We found a significant statistical difference for the visualization of anatomic structures with the 3D and 2D sequences (p < .0001) and no significant statistical difference for the signal-to-noise and contrast-to-noise ratios with the 3D T1-weighted gradient-echo and 2D T1-weighted spin-echo sequences. CONCLUSION: The 3D sequences revealed anatomic structures significantly better than did the 2D sequences and showed pathologic structures considerably more often than did the 2D sequences in all patients. MR imaging of the inner ear and cerebellopontine angle performed with 3D T2-weighted turbo spin-echo and gadolinium-enhanced 3D T1-weighted gradient-echo sequences provided the most accurate imaging leading to diagnosis in cases of abnormality.