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1.
J Environ Manage ; 312: 114913, 2022 Jun 15.
Artículo en Inglés | MEDLINE | ID: mdl-35306418

RESUMEN

A novel electrochemical active biofilms constructed wetland (NEAB-CW) was built to enhance the treatment efficiency for domestic sewage under low temperature environment (0-15 °C). In NEAB-CW, the traditional matrixes were replaced with conductive layer, in which laid stainless steel mesh tubes (SSMT) and added slow-release oxygen matrixes (SROM) and zero-valent iron rod (IR) were used to build a bioelectrochemical activity biofilms system. According to the results of 180 d experiment, the removal efficiencies of COD, NH4+-N and TP of NEAB-CW were 1.52 and 2.21, 2.97 and 1.68, 3.95 and 1.76 times higher than the CW without SROM and IR at 10-20 and 0-10 °C, respectively. The transverse and longitudinal electric potential (EP) variations in NEAB-CW improved microbial activities under low temperature by enhancing the electron transfer efficiency, resulting in higher and stable EP and electron currents density, as well as protein-like contents secreted from biofilms. The pollutant-degrading microorganisms (e.g., Clostridia, Simplicispira), low temperature-resistant microorganisms (e.g., Psychrobacter, Acinetobacter), and electrochemical active microorganisms (e.g., Negativicutes, Gammaproteobacteria) obviously accumulated in NEAB-CW under low temperature environment to generate electricity and degrade pollutants. The results provided a good choice to treat domestic sewage at 0-15 °C by using NEAB-CW.


Asunto(s)
Fuentes de Energía Bioeléctrica , Contaminantes Ambientales , Purificación del Agua , Biopelículas , Aguas del Alcantarillado , Temperatura , Aguas Residuales/química , Humedales
2.
Sci Total Environ ; 881: 163423, 2023 Jul 10.
Artículo en Inglés | MEDLINE | ID: mdl-37062319

RESUMEN

Excessive accumulation of extracellular polymeric substances (EPS) in constructed wetland (CW) substrate can lead to bio-clogging and affect the long-term stable operation of CW. In this study, a microbial fuel cell (MFC) was coupled with air-photocathode to mitigate CW bio-clogging by enhancing the micro-electric field environment. Because TiO2/biochar could catalyze and accelerate oxygen reduction reaction, further promoting the gain of electric energy, the electricity generation of the tandem CW-photocatalytic fuel cell (CW-PFC) reached 90.78 mW m-3. After bio-clogging was mitigated in situ in tandem CW-PFC, the porosity of CW could be restored to about 62.5 % of the initial porosity, and the zeta potential of EPS showed an obvious increase (-14.98 mV). The removal efficiencies of NH4+-N and chemical oxygen demand (COD) in tandem CW-PFC were respectively 31.8 ± 7.2 % and 86.1 ± 6.8 %, higher than those in control system (21.1 ± 11.0 % and 73.3 ± 5.6 %). Tandem CW-PFC could accelerate the degradation of EPS into small molecules (such as aromatic protein) by enhancing the electron transfer. Furthermore, microbiome structure analysis indicated that the enrichment of characteristic microorganisms (Anaerovorax) for degradation of protein-related pollutants, and electroactive bacteria (Geobacter and Trichococcus) promoted EPS degradation and electron transfer. The degradation of EPS might be attributed to the up-regulation of the abundances of carbohydrate and amino acid metabolism. This study provided a promising new strategy for synergic mitigation and prevention of bio-clogging in CW by coupling with MFC and photocatalysis.


Asunto(s)
Fuentes de Energía Bioeléctrica , Humedales , Aguas Residuales , Electrodos , Electricidad
3.
Front Genet ; 13: 851391, 2022.
Artículo en Inglés | MEDLINE | ID: mdl-35571024

RESUMEN

Epidermal growth factor receptor tyrosine kinase inhibitors (EGFR-TKIs) are widely used for patients with EGFR-mutated lung cancer. Despite its initial therapeutic efficacy, most patients eventually develop drug resistance, which leads to a poor prognosis in lung cancer patients. Previous investigations have proved that non-coding RNAs including long non-coding RNAs (lncRNAs), circular RNAs (circRNAs), and microRNAs (miRNAs) contribute to drug resistance by various biological functions, whereas how they regulate EGFR-TKI resistance remains unclear. In this study, we examined gene expression using the microarray technology on gefitinib-resistant NSCLC cells to obtain differentially expressed (DE) lncRNAs and mRNAs. A total of 45 DE-lncRNAs associated with overall survival and 1799 target DE-mRNAs were employed to construct a core lncRNA-miRNA-mRNA network to illustrate underlying molecular mechanisms of how EGFR-TKI resistance occurs in NSCLC. We found that target DE-mRNAs were mainly enriched in pathways involved in EGFR-TKI resistance, especially the target DE-mRNAs regulated by LINC01128 were significantly enriched in the PI3K/Akt signaling pathway, where the synergy of these target DE-mRNAs may play a key role in EGFR-TKI resistance. In addition, downregulated LINC01128, acting as a specific miRNA sponge, decreases PTEN via sponging miR-25-3p. Furthermore, signaling reactions caused by the downregulation of PTEN would activate the PI3K/Akt signaling pathway, which may lead to EGFR-TKI resistance. In addition, a survival analysis indicated the low expression of LINC01128, and PTEN is closely related to poor prognosis in lung adenocarcinoma (LUAD). Therefore, the LINC01128/miR-25-3p/PTEN axis may promote EGFR-TKI resistance via the PI3K/Akt signaling pathway, which provides new insights into the underlying molecular mechanisms of drug resistance to EGFR-TKIs in NSCLC. In addition, our study sheds light on developing novel therapeutic approaches to overcome EGFR-TKI resistance in NSCLC.

4.
Bioresour Technol ; 365: 128142, 2022 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-36257526

RESUMEN

A single-chamber microbial fuel cell-microbial electrolytic cell with a novel constructed wetland system was proposed for synergistic degradation of congo red and reduction in emissions of greenhouse gases. The closed-circuit system showed higher chemical oxygen demand and congo red removal efficiencies by 98 % and 96 % on average, respectively, than traditional constructed wetland. It could also significantly reduce the emissions of CH4 and N2O (about 52 % CO2-equivalents) by increasing the electron transfer. Microbial community analysis demonstrated that the progressive enrichment of dye-degrading microorganisms (Comamonas), electroactive bacteria (Tolumonas, Trichococcus) and denitrifying microorganisms (Dechloromonas) promoted pollutant removal and electron transfer. Based on gene abundance of xenobiotics biodegradation, the congo red biodegradation pathway was described as congo red â†’ naphthalene and alcohols â†’ CO2 and H2O. In summary, the single-chamber closed-circuit system could significantly improve the degradation of congo red and reduce the emissions of greenhouse gases by influencing electron transfer and microbial activity.


Asunto(s)
Gases de Efecto Invernadero , Gases de Efecto Invernadero/análisis , Humedales , Compuestos Azo/metabolismo , Rojo Congo , Dióxido de Carbono/análisis , Óxido Nitroso/análisis , Metano/análisis
5.
Oncol Lett ; 21(2): 122, 2021 Feb.
Artículo en Inglés | MEDLINE | ID: mdl-33552243

RESUMEN

Ovarian cancer (OC) is a common malignant tumor of the female reproductive system. Long non-coding RNAs (lncRNAs) play an important role in OC occurrence and development. Thus, the function and potential mechanism of lncRNA small nucleolar RNA host gene 3 (SNHG3) was explored in the development of OC. The expression of SNHG3, microRNA (miR)-139-5p and Notch homolog 1, translocation-associated (Drosophila) (Notch1) in OC were detected by RT-qPCR or western blot assay. In addition, CCK-8 and wound-healing assays were used to detect OVCAR3 proliferation and migration ability. The targeting relationship of miR-139-5p with SNHG3 or Notch1 was verified through luciferase reporter assay. Rescue experiments were performed to confirm whether SNHG3 could mediate OVCAR3 proliferation and migration through miR-139-5p and Notch1. In OC tissues and cell lines, the expression of SNHG3 and Notch1 were significantly increased, and the expression of miR-139-5p was significantly decreased. SNHG3 inhibition suppressed the proliferation and migration of OVCAR3 cells. Luciferase reporter experiment confirmed that miR-139-5p could target SNHG3 and Notch1. Transfection of miR-139-5p inhibitor significantly reversed the inhibitory effect of SNHG3 knockdown on OVCAR3 proliferation and migration. Moreover, SNHG3 inhibition or miR-139-5p mimic abolished the promotion of Notch1 overexpression on OVCAR3 proliferation and migration. In conclusion, SNHG3 could accelerate the proliferation and migration of OC cells by regulating miR-139-5p and Notch1.

6.
Biosci Rep ; 39(5)2019 05 31.
Artículo en Inglés | MEDLINE | ID: mdl-31028132

RESUMEN

Background Esophageal cancer (ESCA) is one of the most common cancers in the digestive tract. Approximately 300000 people on an average die of ESCA per year worldwide. The determination of key microRNAs for the prognosis of ESCA is of indispensable significance in the clinical treatment. Methods The differentially expressed microRNAs were screened by analyzing The Cancer Genome Atlas (TCGA) database. By using the survival data of the database, we analyzed correlation between patients' survival time and miR-550a expression levels. Differential expression analysis and gene set enrichment analysis were performed using the targeted data. Results It was found that patients with high miR-550a expression levels had shorter survival time. Data mining and signal pathway enrichment analysis of TCGA database showed that abnormal miR-550a expressions affected the recurrence of tumors by the muscle system regulation. Conclusions Through the proposed investigation, miR-550a is found to be a potential biomarker as well as non-coding therapeutic target for esophagus cancer. These results suggest that miR-550a may serve as a therapeutic target and predictor for ESCA survival.


Asunto(s)
Biomarcadores de Tumor/metabolismo , Bases de Datos de Ácidos Nucleicos , Neoplasias Esofágicas/metabolismo , Neoplasias Esofágicas/mortalidad , MicroARNs/metabolismo , ARN Neoplásico/metabolismo , Biomarcadores de Tumor/genética , Minería de Datos , Supervivencia sin Enfermedad , Neoplasias Esofágicas/genética , Femenino , Humanos , MicroARNs/genética , Músculos/metabolismo , ARN Neoplásico/genética , Tasa de Supervivencia
7.
Phys Med Biol ; 62(5): 1810-1830, 2017 03 07.
Artículo en Inglés | MEDLINE | ID: mdl-28052045

RESUMEN

The beam hardening effect can induce strong artifacts in CT images, which result in severely deteriorated image quality with incorrect intensities (CT numbers). This paper develops an effective and efficient beam hardening correction algorithm incorporated in a filtered back-projection based maximum a posteriori (BHC-FMAP). In the proposed algorithm, the beam hardening effect is modeled and incorporated into the forward-projection of the MAP to suppress beam hardening induced artifacts, and the image update process is performed by Feldkamp-Davis-Kress method based back-projection to speed up the convergence. The proposed BHC-FMAP approach does not require information about the beam spectrum or the material properties, or any additional segmentation operation. The proposed method was qualitatively and quantitatively evaluated using both phantom and animal projection data. The experimental results demonstrate that the BHC-FMAP method can efficiently provide a good correction of beam hardening induced artefacts.


Asunto(s)
Algoritmos , Intensificación de Imagen Radiográfica/métodos , Tomografía Computarizada por Rayos X/métodos , Artefactos , Fantasmas de Imagen , Tomografía Computarizada por Rayos X/normas
8.
Artículo en Zh | WPRIM | ID: wpr-821171

RESUMEN

@#[Abstract] Objective: To investigate the effects of forkhead box transcription factor (FOXK2) overexpression on the proliferation, migration, invasion and adhesion of human ovarian cancer SK-OV-3 cells and its related molecular mechanism. Methods: The open reading frame (ORF) of FOXK2 was cloned into lentivirus expression vector, which was then enveloped in HEK293T cells and transfected into human ovarian cancerSK-OV-3cells.TheoverexpressionefficiencywasdetectedbyqPCRandWesternblotting.Theproliferation, migration, invasion and adhesion of SK-OV-3 cells were detected by CCK-8, Scratch-healing, Transwell and Cell adhesion assays respectively, and the expressions of epithelial-mesenchymal transition (EMT) markers were detected by qPCR. Results: The FOXK2 overexpression vector was constructed successfully and packaged into lentivirus, which was then transfected into SK-OV-3 cells. After transfection, the expression of FOXK2 was significantly increased (P<0.01); the proliferation, migration and invasion of SK-OV-3 cells were significantly reduced while the adhesion ability was significantly increased (P<0.05 or P<0.01); and the expression levels of E-cadherin and β-catenin were significantly increased while that of vimentin and fibronection were significantly decreased (all P<0.01). Conclusion: Overexpression of FOXK2 in SK-OV-3 cells leads to a significant decrease in proliferation, migration and invasion but increase in adhesion. The molecular mechanism may be related to the reversion of the EMT process in tumor cells, suggesting that FOXK2 may be a potential target for the diagnosis and treatment of ovarian cancer.

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