Simultaneous inhibition of XIAP and survivin expression on EMT and invasion of human pancreatic cancer cells / 中南大学学报(医学版)

OBJECTIVE@#To investigate the simultaneous inhibition of X-linked inhibitor of apoptosis protein (XIAP) and survivin expression on epithelial-mesenchymal transition (EMT) and invasiion of pancreatic cancer cells Panc-1, and its mechanism.@*METHODS@#On the established human pancreatic cancer cells Panc-1-XS, the expression of XIAP and survivin was inhibited simultaneously. Cell invasion and migration were detected by Transwell chamber experiments and scratch test, and the expression of epithelial marker E-cadherin, mesenchymal markers Slug, phosphatase and tensin homolog deleted on chromosome ten (PTEN) and P-Akt protein was determined by Western blot.@*RESULTS@#Cell invasion and migration of Panc-1-XS cells decreased significantly, accompanied by significantly upregulated protein expression of E-cadherin, and significantly declined protein expression of the Slug, indicating increased mesenchymal-epithelial conversion (MET); and increased protein expression of PTEN, and declined protein expression of P-Akt.@*CONCLUSION@#Simultaneously inhibiting the expression of XIAP and survivin can partially reverse EMT phenotype of pancreatic cancer Panc-1 cells, which then significantly reduces the cell invasion and migration of Panc-1 cell lines. This process may be regulated by PTEN/PI3K/Akt signaling pathway.

Experimental study of low molecular weight heparin inducing acinar cell apoptosis in severe acute pancreatitis / 中国医师杂志

Objective To explore the mechanism of LMWH therapy for SAP.Methods 48 wistar rats were random divided into 3 groups,sham group(S group),severe acute pancreatitis group(SAP group)and LMWH therapy group(H group).Serum amylase,IL-6,acinar cell apoptosis and the activity of NF-κB were detected and compared.Results The expression of amylase and IL-6 in SAP group was significantly higher than that in H group(P＜0.01).The apoptosis index of acinar cell in SAP group wag significantly lower than that in H group(P＜0.01),while the activity of NF-κB in SAP groupwas stronger than that in H group.Conclusions LMWH therapy may ameliorate SAP by inducing acinar cell apoptosis through suppressing the activity of NF-κB.

Autologous vein graft as a conduit for repairing the recurrent laryngeal nerve deficit / 中国普通外科杂志

Objective To evaluate the result of autologous vein graft used as a conduit for repairing the iatrogenic recurrent laryngeal nerve(RLN) deficit.Methods Iatrogenic RLN deficit was repaired by using antogeneous vein graft as a conduit in 17 patients. RLN deficit occurred in right side in 5 cases, in left side in 12. Results The RLN injuries were located within 2 cm below the point of RLN entering throat in 16 cases(94 1%), near the inferior thyroid artery in 1(5 9%). The length of RLN deficit was between 0 5～4 cm. Among the 17 cases, 16(94 1%) were followed up for more than 1 5 years. Of them, normal phonation was achieved in 12 cases(75 0%), significantly improved in 3(18 8%) and slightly improved in 1(6 2%).Among the 15 cases who received indirect or direct laryngoscopy, the affected vocal cord movement entirely recovered in 4 cases(26 7%), partially recovered in 3(20 0%), unchanged in 8(53 3%) Conclusions Autogeneous vein graft is ideal for repairing RLN deficit.

Problems concerning the diagnosis and treatment of thyroid cancer / 中国普通外科杂志

Objective To study the approach to improving the diagnosis and treatment of thyroid cancer(TC). Methods Problems regarding to the diagnosis and treatment of TC had arisen based on the combination of auther's clinical experience and recent literatures review. Results and Conclusions (1) In the diagnosis of TC, attention should be paid to the following conditions: cystocarcinoma, multicentral cancer, bilateral TC, coexist of benign thyroid diseases with TC, and the microcancer of thyroid etc. (2) Biopsy should be done during and after operation for the thyroid nodule needed to be operated, to avoid misdiagnosis of TC. (3) Except undifferentiated carcinoma, the choice of treatment of TC is surgery. The choice of operative procedure should be according to the pathological type and clinical stage of TC, and patient's condition. (4) Thyroid hormone suppression therapy should be administrated after operation. (5) Posterative radiotherapy can promote the long term survival rate. (6) Reoperation should be performed immediately, if the thyroid nodule is TC and comfired by pathological examination posteratively, and a subtotal thyroidectomy or nodule local resection performed on for that nodule initially, to avoid the residual cancer and cancer recurrence in the residual thyroid tissues.

Caspase 3 Activity of Ischemia Reperfusion Injury in Rats and The Protective Mechanism of Ischemic Preconditioning / 中国医师杂志

Objective To investigate Caspase 3 activity in the ischemia reperfusion injury in rats and the protective effect of ischemic preconditioning and its possible mechanism.Methods 30 rats were randomly assigned to ischemia reperfusion (IR) group, ischemic preconditioning(IP) group,sham-operation(S)group.The serum aspartate transaminase (AST),alanine transaminase(ALT),liver caspase 3 activity,and apoptosis index(AI) of hepatocytes were examined in the three groups at 3 hours after repersusion.Results The serum AST,ALT,liver caspase 3 activity and apoptotic hepatocytes were significantly higher in both IP and IR groups than those in S group(P

Clinical study on the effect of normothermic liver ischemic preconditioning on the expression of apoptosis regulating genes / 中国普通外科杂志

Objective To explore the expression of apoptosis regulating genes C jun and bcl X L after normothermic liver ischemic preconditioning and its clinical significance. Methods 16 cases of liver cancer were randomly divided into ischemic reperfusion(IR) group and ischemic preconditioning (IP) group (8 cases in each). The samples of venous blood were drawn before IR or IP procedure and 30 minute after reperfusion for testing ALT, AST and LDH. Meanwhile, samples of liver tissue were taken for study of hepatocellular apoptosis, expressions of C-jun mRNA、 Bcl-X L mRNA and PCNA and morphologic changes. Results The levels of ALT、 AST、 LDH and AI in IR group were significantly higher than those in IP group (P

The influence of RhoC on the expression of angiogenic factors in HCC cells / 中国普通外科杂志

Objective To study the influence of pcDNA3.1-RhoC on the expression of endogenous angiogenic factors in HCC cells.Methods The reconstructed plasmid pcDNA3.1-RhoC was transfected into HepG2 cells, and expression of VEGF and bFGF was detected with the RT-PCR and immunohistochemical stain. HepG2 cells transfected with pcDNA3.1-RhoC or pcDNA3.1 were implanted into nude mice to observe the tumor occurrence rate.Results HepG2 cells transfected with pcDNA3.1/RhoC showed higher expression of RhoC . The expression of RhoC enhanced the expression of VEGF and bFGF(P

Treatment of Hepatic Cavernous Hemangioma:A Report of 47 Cases / 中国医师杂志

Objective To summrize the effects of various treatment methods,including operation,transcatheter arterial embolization(TAE),and percutaneous ethanol injection(PEI) on hepatic cavernous hemangioma(HCH),in order to select an optimal therapy strategy for HCH.MethodsClinic data of 47 patients with 58 hepatic hemangiomas treated in our hospital in recent 10 years were analyzed retrospectively.Results There were 26 males and 21 females with mean age 42?2 5 years(10～72 years old)in this series.The diameters of these lesions were 3～21 5cm(mean diameter 8 5?1 8cm).Of them,obvious symptoms appeared in 39 patients,malignancy could not excluded in 6,and the size of HCH increased rapidly in 2.Operations were performed in 40 patients,including 2 patients failed to PEI;TAE was conducted in 7cases.There was no mortality or major morbidity occurred in the operative patients,except right pleual effusion in one case and subdiaphragmatic fluid collections in another case(2/40).Neither is mortality nor morbidity seen in the TAE treated case.Conclusions Operation is an effective procedure for HCH.But for more diffuse lesions or lesions located in special region,TAE is a more favorable alterative selection.Because PEI has risk of hemorrhage and hardener injection under ultrasonography guide has uncertain outcome,so it should be chosen cautiously.

Ultrastructure Alteration of the Gallbladder in Rabbits With Gallstone Before and After Cholecystolithotomy / 中国医师杂志

Objective To explore the relationship between the ultrastructure and gallstone recurrence after cholecystolithotomy.Methods By implanting human cholesterol gallstone into the gallbladder of rabbits, the ultrasructure of the gallbladder was observed with scanning eleetron microscope and transmission electron microscope,3 months before and after cholecystolithotomy.Results ln the stone-implanted group,the epithelium cells were massively damaged,nuclei karyopyknosis,mitochondria destroyed, and phagolysosomes increased. After the gallstone removal, the epitheliune cells did not recover completely where exposed connective tissues and covering surface by mucus. In cytoplasm, the lysosome decreased, and mucus granules secretion increased and actively secreted into the gallbladder lumen.Conclusions The pathologic changes of chronic cholecystitis caused by implanting stones in rabbits is similar to that of the chronic calculous cholecystitis in human.The basic cause of gallstone recurrence after cholecystolithotomy might be that the ultrastructural changes not be restored and the mucus secretion increased obviously in gallbladder.

Clinical Study of Treatment of Low Molecular Weight Heparin / 中国医师杂志

Objective To explore the treatment effects of low molecular weight heparine (LMWH) on severe acute pancreatitis (SAP). Methods Random analysis were made on the clinical features and therapeutic effect on the control treatment group (CT) in 65 cases and LMWH treatment group (LT) in 77 cases.Results 70(90 9%) cases were cured in LT group,and 48(73 8%) cases were cured in CT group. The incidence of complications in the LT group (28 6%, 22patients 45 times) was significantly lower than that in the CT group (60%,39patients 79 times)(P

The Effect of Nitric Oxide on IL -1? and TNF-? in Liver Ischemic Precondition in Rats / 中国医师杂志

Objective To study the effects of nitric oxide (NO) synthesis on serum interleukin 1? (IL-1?) and tumor necrosis factor ? (TNF-?) levels in liver ischemic preconditioning (IP) in rats. Methods 169 rats were randomly divided into ischemia/reperfusion (I/R) group, IP group,L-arginine plus IP (L-Arg+IP) group and sham operation group (S group). The concentrations of plasma NO, serum IL-1? and TNF-? were measured at 2h, 24h and 1 week after models were set up. Results ⑴ Plasma NO concentrations: In group IP and group L-Arg+IP,the NO concentration at all the 3 time points were significantly higher than those in group S (P0 05). Conclusions Liver ischemic preconditioning could down-regulate the levels of blood IL-1? and TNF-?, which may relate to the increase of NO synthesis. The increase of NO synthesis could enlarge this down-regulation effect, and further enhance the protective effect of IP on the liver I/R injury.

Comparison of the protective effect of ischemic preconditioning and Caspase inhibitor on ischemia reperfusion injury of the liver in rats / 中国普通外科杂志

Objective To compare the protective effect of ischemic preconditioning(IP) and caspase inhibitor on ischemia reperfusion(IR) injury of the liver in rats,and to investigate the possible mechanism. Methods SD rats subjected to 60 or 120 minutes of 70% hepatic ischemia and 3 hours of reperfusion,respectively,then divided randomly into 6 groups: (1)Ischemia/ reperfusion group 1 (IR 1 group);(2)IR 2 group;(3)Ischemia preconditioning group 1(IP 1 group);(4)IP 2 group; (5)Caspase inhibitor treatment group1(T 1 group);(6)T 2 group.Serum levels of aspartate aminotransferase (AST) and alanine aminotransfernase (ALT),and liver Caspase-3 activity, hepatocytes apoptosis,and animal seven-day(7 d) survive rate were determined. Results Both ischemic preconditioning and Caspase inhibitor treatment could protect the liver against IR injury,and their protective effect in IP 1 group and T 1 group was equally at 3h reperfusion after 60min ischemia. At 3h reperfusion after 120min ischemia, the effect of inhibiting Caspase-3 activity and decreasing hepatocyte apoptosis in T 2 group were significantly superior to those in IP 2 group(P0.05). Conclusions Both IP and Caspase inhibitor treatment can protect the liver against IR injury in rats,and the protective effect is no significant difference between the two methods.However IP is more simple?safe and cheaper comparing with Caspase inhibitor treatment.

Clinical application of ischemic preconditioning on patients with primary hepatic carcinoma underging hepatectomy / 中国普通外科杂志

0.05).(2)Serum ALT,AST and LDH:After reperfusion,the values of ALT,AST,and LDH in IP group were significantly lower than those in I-R group(P

The Clinical Study of Kansui Root Therapy for Severe Acute Pancereatitis / 中国医师杂志

0.05); ⑵Serum TNF? and IL-6 levels in kansui root group were significantly lower than those in control group at the third, 7th and 14th days after treatment(P0.05). Conclusion ⑴kansui root therapy was effective for SAP, ⑵kansui root therapy ameliorated SAP possibly by decreasing the levels of serum TNF? and IL-6, ⑶kansui root therapy improved microcirculation of pancreas possibly by decreasing the ratio of TXA 2/PGI 2, and ⑷kansui root treating SAP may be multi-pathway and multi-target.

Experimental Study of Low Molecular Weight Heparin Therapy for Kidney Injury in Severe Acute Pancreatitis / 中国医师杂志

Objective To investigate the effect of low molecular weight heparin (LMWH) on kidney injury in severe acute pancreatitis (SAP). Methods SD rats were randomly divided into 3 groups: Sham operation (S) group,SAP group, and LMWH treatment (LT) group. In LT group LMWH was administrated 4 hours after SAP model establishment. The levels of serum amylase, creatinine (Cr) and tumor necrosis factor alpha (TNF-?), renal morphological change, and the expression of p65 mRNA and protein in renal specimens were determined 24 hours after model establishment or LMWH treatment. Results The levels of serum amylase,Cr,TNF-? in SAP group were significantly higher than those in S group and LT group (all P0.05). The expression of p65 protein and mRNA of renal tissues in SAP group was significantly higher than that in S group and LT group (all P

Treatment of spontenous rupture of hepatocellular carcinoma(a report of 64 cases) / 中国普通外科杂志

Objective To study the treatment of sponteneous rupture of hepatocellular carcinoma(SRHCC).Methods The clinical data of 64 patients with SRHCC treated by non-operation or operation were analysed retrospectively.Results The mean survival time of 14 patients treated by conservative therapy was 7 days(12 hours～12 days), the mean surival time of 31 patients treated by packing and 6 patients treated by ligation of hepatic artery was 11 days(4 hours-3 months)and 2.5 months(8 days-6 months);and the 1-month survival rate was 3.2% and 66.7% respectively.1 patient underwent transhepatic artery embolism surviving 9 days.The 1-year survival rate of 12 patients treated by hepatectomy was 66.7%;the mean survival time was 36 months(4 days-25 years and 3 months).Of the 12 patients,11 survived for over one and a half months whose liver function was Child-Pugh grade A before operation; 1 died of liver function failure 4 days after operation,whose liver function was Child-Pugh grade B before operation.Conclusions Emergent hepatectomy should be reserved for patients with an easily resectable lesion whose liver function is in Child-Pugh grade A.Early two-stage or delayed hepatectomy would be suitable for those patients who are unsuitable for emergent operation.Conservative therapy and other therapies may be used for patients with terminal stage of SRHCC.

Low molecular weight heparin in the treatment of severe acute pancreatitis / 中国普通外科杂志

Objective To investigate the effects of low molecular weight heparin (LMWH) on severe acute pancreatitis(SAP). Methods Retrospective analysis was made on the clinical features and therapeutic effect on the routine treatment (RT) group in 48 cases and LMWH treatment(LT) group in 31 cases. Results 28 (90.3%) out of 31 cases were cured while 3 (9.7%) died in LT group. 35(72.9%) out of 48 cases were cured whereas 15 (27.1%) died in RT group. The complications occurring in LT group (32.3%,10 patients 18 times ) was significantly lower than that in the RT group (62.5%,30 patients 59 times)(P

Primary abdominal aortic aneurysm-intestinal fistula(a case report and literature review) / 中国普通外科杂志

Objective To study the diagnosis and treatment of abdominal aortic anurysm-intestinal fistula(AAA-IF). Methods The diagnosis and treatment of one patient with AAA-IF was reported, while a literateres review of AAA-IF was made. Results A 50 years old femal patient abmitted due to upper abdominal pain for two month, and an AAA was found in the explore laparotomy. The patient suddenly occurred severe upper abdominal pain accompanied with shock and massive upper gastrointestinal bleeding 13 days after the operation. The preoperative suspected diagnosis was AAA-IF. An AAA-IF was ound during emergent operation. AAA-IF resection with artifial vessel replacement, resection of the forth portion of duodenal and upper portion of jejunal were performed together. The patient recovered eventurely except high body temperature. After vigovous antibiotics therapy, the patient recovered. Conclusions AAA-IF is a rare disordor, the mortality can be as high as over 30%. The key points of redecing mortality are that the diagnosis and treatment should be in time; postoperative vigorous antibiotics should be given, because the AAA-IF patient tends to occur septic complications due to the communication occurred between the AAA and intestinal tract.

Construction of RhoC gene vector and its expression in eucaryotic cells / 中国普通外科杂志

Objective To establish an experimental model for exploring the role of RhoC gene in the invasiveness and metastasis of hepatocellular carcinoma.Method The RhoC gene was digested with restricted enzyme Hind III and XbaI,and direct cloned to pcDNA3.1.The recombinant vector (pcDNA3.1 RhoC) and the vector alone (pcDNA3.1) were transfected into HEPG2 cells with LIPFECTAMINETMReagent.After selected with hygromycin,resistant cloneies was obtained.The transcription and translation of RhoC gene were analysed with the reverse transcription PCR and immunohistochemical stain.Results The recombinant vectort (pc DNA3.1 Rhoc) express steadily in HerpG2 cells.Conclusions The modified tumor cells(HEPG2 RhoC) could be used to study the effect of RhoC protein on the invasiveness and metastasis of hepatocellular carcinoma.

Influence of obstructive jaundice on macrophage activity and bacterial translocation / 中国普通外科杂志

Objective To investigate the influence of bile duct ligation ( BDL ) on macrophage ( M ) activity and bacterial translocation in mice . Methods After 7 days of BDL , the levels of serum total bilirubin(TB) , albumin(Alb) , glutamic - oxaloacetic transaminase(AST) , creatinine(Cr) , alkaline phosphatase(ALP) and nitrogen oxide(NO) producted by peritoneal macrophages were measured . The mesenteric lymph nodes were cultured for bacteria . The liver and kidney tissues were histopathologically examined. Results The levels of serum iTB,AST,AKP in BDL group were significantly higher than those in control group(P