AQP4 regulates ferroptosis and oxidative stress of Muller cells in diabetic retinopathy by regulating TRPV4.

Diabetic retinopathy (DR) is a common microvascular complication that causes visual impairment or loss. Aquaporin 4 (AQP4) is a regulatory protein involved in water transport and metabolism. In previous studies, we found that AQP4 is related to hypoxia injury in Muller cells. Transient receptor potential cation channel subfamily V member 4 (TRPV4) is a non-selective cation channel protein involved in the regulation of a variety of ophthalmic diseases. However, the effects of AQP4 and TRPV4 on ferroptosis and oxidative stress in high glucose (HG)-treated Muller cells are unclear. In this study, we investigated the functions of AQP4 and TRPV4 in DR. HG was used to treat mouse Muller cells. Reverse transcription quantitative polymerase chain reaction was used to measure AQP4 mRNA expression. Western blotting was used to detect the protein levels of AQP4, PTGS2, GPX4, and TRPV4. Cell count kit-8, flow cytometry, 5,5',6,6'-tetrachloro-1,1,3,3'-tetraethylbenzimidazolyl carbocyanine iodide staining, and glutathione (GSH), superoxide dismutase (SOD), and malondialdehyde (MDA) kits were used to evaluate the function of the Muller cells. Streptozotocin was used to induce DR in rats. Haematoxylin and eosin staining was performed to stain the retina of rats. GSH, SOD, and MDA detection kits, immunofluorescence, and flow cytometry assays were performed to study the function of AQP4 and TRPV4 in DR rats. Results found that AQP4 and TRPV4 were overexpressed in HG-induced Muller cells and streptozotocin-induced DR rats. AQP4 inhibition promoted proliferation and cell cycle progression, repressed cell apoptosis, ferroptosis, and oxidative stress, and alleviated retinal injury in DR rats. Mechanistically, AQP4 positively regulated TRPV4 expression. Overexpression of TRPV4 enhanced ferroptosis and oxidative stress in HG-treated Muller cells, and inhibition of TRPV4 had a protective effect on DR-induced retinal injury in rats. In conclusion, inhibition of AQP4 inhibits the ferroptosis and oxidative stress in Muller cells by downregulating TRPV4, which may be a potential target for DR therapy.

DEAR model in overweight endometrial cancer patients undergoing fertility-sparing treatment: A randomized controlled trial.

OBJECTIVE: To evaluate the effects of DEAR weight management in overweight patients undergoing fertility-sparing treatment for endometrial cancer or atypical hyperplasia. METHODS: Women with endometrial cancer or atypical hyperplasia who received fertility-sparing treatment and had a body mass index of >25 kg/m2 were randomly allocated to the DEAR (DEAR weight management) and control (self weight management) groups. Body morphology and composition, glycolipid metabolism, and tumor outcomes were assessed in both groups before and at 3 and 6 months after intervention. RESULTS: Overall, 72 subjects were included (36 in each group). Following intervention, the DEAR group showed significantly lower median body weight (69.45 vs. 78.05), body mass index (26.19 vs. 29.15), lipid accumulation index (29.21 vs. 57.86), body fat mass (24.00 vs. 29.30), visceral fat area (112.5 vs. 133.3), and glycolipid metabolic indices (except high density lipoprotein) than the control group (P < 0.05) and showed a decreasing trend. The test group achieved significantly higher complete remission (88.46% vs. 57.14%; P < 0.05); the time to complete remission did not differ significantly (P > 0.05). CONCLUSIONS: DEAR weight management can improve the studied parameters and complete remission rates in this population. REGISTRATION: NCT06169449.

Surface-engineered Mo2B: a promising electrode material for constructing Ohmic contacts with blue phosphorene for electronic device applications.

The Schottky barrier between a metal and a semiconductor plays an important role in determining the transport efficiency of carriers and improving the performance of devices. In this work, we systematically studied the structure and electronic properties of heterostructures of blue phosphorene (BP) in contact with Mo2B based on density functional theory. The semiconductor properties of BP are destroyed owing to strong interaction with bare Mo2B. The effect of modifying Mo2B with O and OH on the contact properties was investigated. A p-type Schottky contact can be obtained in BP/Mo2BO2. The height of the Schottky barrier can be modulated by interlayer distance to realize a transition from a p-type Schottky contact to a p-type Ohmic contact in BP/Mo2BO2. The BP/Mo2B(OH)2 forms robust Ohmic contacts, which are insensitive to interlayer distance and external electric fields due to the Fermi level pinning effect. Our work provides important clues for contact engineering and improvement of device performance based on BP.

Increased TIGIT expressing NK cells with dysfunctional phenotype in AML patients correlated with poor prognosis.

AML is the most common blood cancer in adults with a high relapse and an overall poor survival rate. NK cells have been demonstrated to have the capacity to eradicate AML blast, and an impaired NK cell function is involved in AML development and progression. Immune checkpoints are involved in immune escape in various cancers. Immune checkpoints blockade therapy mainly aimed to unleash CD8+T cells function, but NK cells have emerged as new target. However, immune checkpoints profile on NK cells has not been observed in AML patients. Here, we studied the immune checkpoints expression of NK cells from AML patients at initial diagnosis and found increased PD-1, TIGIT and TIM-3 expression compared to NK cells from healthy donors. Further analysis showed that TIGIT expressing NK cells from AML patients had a dysfunctional phenotype, as TIGIT+NK cells exhibit lower antileukemia effect, cytokine production and degranulation compared to TIGIT-NK cells. TIGIT blockade could significantly enhance the function of NK cells. Moreover, AML patients with high frequency of TIGIT+NK cells had higher frequency of poor prognosis risk. Further analysis found that IL-10 upregulated TIGIT expression on NK cells. Thus, TIGIT blockade alone or in combination with other therapy might be potential strategy to treat AML.

Electrosynthesis of Ionic Covalent Organic Frameworks for Charge-Selective Separation of Molecules.

Covalent organic frameworks (COFs) have emerged as potent material platforms for engineering advanced membranes to tackle challenging separation demands. However, the synthesis of COF membranes is currently hampered by suboptimal productivity and harsh synthesis conditions, especially for ionic COFs with perdurable charges. Herein, ionic COFs with charged nanochannels are electrically synthesized on conductive supports to rapidly construct composite membranes for charge-selective separations of small molecules. The intrinsic charging nature and strong charge intensity of ionic COFs are demonstrated to collectively dominate the membrane growth. Spontaneous repairing to diminish defects under the applied electric field is observed, in favor of generating well-grown COF membranes. Altering electrosynthetic conditions realizes the precise control over the membrane thickness and thus the separation ability. Electrically synthesized ionic COF membranes exhibit remarkable molecular separation performances due to their relatively ordered and charged nanochannels. With these charge-selective pathways, the membranes enable the efficient sieving of charged and neutral molecules with analogous structures. This study reveals an electrical route to synthesizing COF thin films, and showcases the great potential of ionic nanochannels in precise separation based on charge selectivity.

LncRNA MALAT1 aggravates the retinal angiogenesis via miR-320a/HIF-1α axis in diabetic retinopathy.

Diabetic retinopathy (DR) is one of the most serious microvascular complications of diabetes and an important cause of blindness in adults. In previous study, we found that miR-320a alleviated the damage of muller cells in DR. In this study, we mainly explored the mechanism of lncRNA MALAT1 on retinal angiogenesis in DR by regulating miR-320a/HIF-1α. The expression of MALAT1 and miR-320a was detected by RT-qPCR, and the expression of HIF-1α was detected by Western blot. The superoxide anion level, invasion, angiogenesis, and vascular permeability of mouse retinal microvascular endothelial cells (MRMECs) co-cultured with muller cells were evaluated by dihydroethidium, transwell, angiogenesis and immunofluorescence assay. In order to analyze the targeting relationship between miR-320a and MALAT1 or HIF-1α, we performed dual luciferase reporter gene, fluorescence in situ hybridization (FISH), RNA immunoprecipitation (RIP) and RNA pulldown experiments. The results should that MALAT1 and HIF-1α were highly expressed and miR-320a was low expressed in high glucose (HG)-induced muller cells, and MALAT1 could competitively bind with HIF-1α. Knocking down miR-320a inhibited MRMECs invasion angiogenesis, and vascular permeability by targeting miR-320a. Overexpression of miR-320a down regulated HIF-1α and inhibited the invasion, angiogenesis, and vascular permeability of MRMECs. In conclusion, MALAT1 inhibits HIF-1α expression and MRMECs angiogenesis in DR through spongy miR-320a.

A theoretical study of laser-induced ultrafast spin dynamics in trigonal monopyramidal iron and nickel complexes.

We present a first-principles study of the geometries, electronic structures, and laser-induced ultrafast spin dynamics in four trigonal monopyramidal complexes [tpat-BuFe]-, [tcmat-BuFe]-, [tpat-BuNi]-, and [tcmat-BuNi]- [tpa: tris-(pyrrolylmethyl)amine; tcma: tris(carbamoyl-methyl)amine; t-Bu: tert-butyl]. It is found that the low-lying level distribution of the four structures is similar, however, their spin and charge localization differs substantially. Detailed analysis demonstrates that the iron complexes have much more singly spin localized states located in the low energy region, while the nickel complexes have more charge-transfer (CT) states and more states with spin equally distributed between the Ni and the ligands. Affected by these features, more ultrafast spin-crossover (SCO) scenarios are achieved in the two iron complexes, and better CT dynamics is obtained in nickel complexes. In particular, for the CT scenarios combined with spin bifurcation, the charge is transferred from the tpa/tcma ligand to the Fe/Ni atoms, while spin-density transfer occurs in the opposite direction. Among the scenarios illustrated in the paper, the SCO processes turn out to be more complicated since they involve many more intermediate states and exhibit relatively low fidelity. In addition, the transferability of each scenario is analyzed from the absorption spectra of the initial and final states. All these results can provide significant insights into the electronic and magnetic natures of the four complexes, guide the experimental realization of the relevant scenarios, and thus promote their applications in molecular spintronics.

A UCMPs@MIL-100 based thermo-sensitive molecularly imprinted fluorescence sensor for effective detection of ß-lactoglobulin allergen in milk products.

In this study, a thermo-sensitive molecularly imprinted fluorescence sensor was developed for the specific detection of ß-Lactoglobulin (ß-LG) allergen in milk products. The metal-organic frameworks (MIL-100) with a high specific surface area was coated on the surface of upconversion micro-particles (UCMPs). As the core, an imprinted polymer layer allowing for swelling and shrinking with response to temperature was prepared, which exhibited high adsorption and mass transfer capabilities for ß-LG allergen. The fluorescence intensity of UCMPs@MIL-100@MIP decreased linearly with the concentration of ß-LG in the range of 0.1-0.8 mg mL-1, and the limit of detection was 0.043 mg mL-1. The imprinting factor reached 3.415, which indicated that excellent specificity of the UCMPs@MIL-100@MIP for ß-LG allergen. In the analysis of ß-LG allergen in actual milk samples, the proposed UCMPs@MIL-100@MIP fluorescence sensor produced reliable and accurate results (recovery: 86.0-98.4%, RSD: 2.8-6.8%), closely related to the results of standard HPLC method (correlation coefficient: 0.9949), indicating that its feasibility in the detection of ß-LG allergen.

M2 macrophages secrete CXCL13 to promote renal cell carcinoma migration, invasion, and EMT.

OBJECTIVE: M2 macrophages are associated with a poor prognosis in a variety of malignancies. There are, however, few relevant investigations in clear cell renal cell carcinoma (ccRCC). METHODS: The expression of M2 macrophages in ccRCC tissues was first discovered using immunohistochemistry in this study. Then, M2 macrophages were created in vitro to see how they affected the proliferation, migration, invasion, and EMT of ccRCC cells. Using qPCR and prognostic analysis identifies important chemokine. Antibody neutralization tests confirmed the chemokine's involvement and function. Pathway inhibitors confirmed the main pathway of M2 macrophages in ccRCC. Finally, qPCR and IHC were used to confirm the expression of chemokine receptors in ccRCC tissues. RESULTS: The presence of M2 macrophages was linked to a poor outcome in ccRCC. M2 macrophages enhanced the proliferation, migration, invasion, and EMT of ccRCC lines in vitro. CXCL13 was identified as the main chemokine by prognostic analysis and qPCR tests. CXCL13 neutralizing antibodies can inhibit the stimulation of M2 macrophages in ccRCC lines' proliferation, migration, invasion, and EMT. M2 macrophages and CXCL13 may activate the Akt pathway in ccRCC lines, and Akt inhibitors decrease ccRCC lines proliferation, migration, invasion, and EMT. CXCR5 expression is a poor prognostic factor for renal cell carcinoma, according to qPCR and immunohistochemistry. In vivo experiments further proved that CXCL13 secreted by M2 macrophages can promote tumor proliferation. CONCLUSIONS: M2 macrophages in the immunological milieu secrete CXCL13, which promotes ccRCC proliferation, migration, invasion, and EMT. Our findings contribute to a better understanding of the function of the tumor microenvironment in the incidence and progression of ccRCC, and they may point to novel therapeutic targets for ccRCC.

microRNA-320a prevent Müller cells from hypoxia injury by targeting aquaporin-4.

Müller cells are closely related to diabetic retinopathy (DR). Aquaporin-4 (AQP4) can effectively promote the diffusion of water across cellular membranes. However, the dynamic balance of water plays key role in many diseases, such as cerebral edema. Meanwhile, the unusual expression and distribution of AQP4 in the retina are the significant causes of ocular hypertension and reperfusion injury. To explore the functional significance between microRNA-320a (miR-320a) and AQP4 in pathological hypoxia-induced DR related retinal edema, we hypothesized that miR-320a regulates AQP4 expression and internalization to relieve the edema of Müller cells under the pathological retinal hypoxia stress by targeting AQP4, thereby attenuate the damage of Müller cells. Results demonstrated that miR-320a mimics inhibited the expressions of AQP4 in Müller cells. Furthermore, overexpression miR-320a protected Müller cells by suppressing superoxide anion. In addition, overexpression miR-320a markedly attenuated hypoxia-induced injury, significantly increased the cell viability, and promoted the internalization of AQP4. Furthermore, miR-320a can also regulate the stable anchoring of AQP4 on the cell membrane. Our study indicated that miR-320a may be a potential modulator which can mediate AQP4 expression and attenuate the hypoxia damage of Müller cells. In conclusion, miR-320a may be a potential target for DR therapy by targeting AQP4.

Fabrication and evaluation of a label-free piezoelectric immunosensor for sensitive and selective detection of amantadine in foods of animal origin.

A label-free piezoelectric immunosensor was fabricated and applied to the detection of the antiviral drug amantadine (AM) in foods of animal origin. Experimental parameters associated with the fabrication and measurement process were optimized and are discussed here in detail. The proposed piezoelectric sensor is based on an immunosuppression format and uses a portable quartz crystal microbalance (QCM) chip. It was found to provide a good response to AM, with a sensitivity and limit of detection (LOD) of 33.9 and 1.3 ng mL-1, respectively, as well as low cross-reactivity (CR, < 0.01%) with AM analogues. The immunosensor was further applied to quantify AM at three levels in spiked samples of typical foods of animal origin, and yielded recoveries of 83.2-93.4% and standard deviations (SDs, n = 3) of 2.4-4.5%, which are comparable to the results (recoveries: 82.6-94.3%; SDs: 1.7-4.2%) obtained using a high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) method. Furthermore, the piezoelectric immunosensing chip can be regenerated multiple (at least 20) times with low signal attenuation (about 10%). A sample analysis can be completed within 50 min (sample pretreatment: about 40 min, QCM measurement: 5 min). These results demonstrate that the developed piezoelectric immunosensor provides a sensitive, accurate, portable, and low-cost analytical strategy for the antiviral drug AM in foods of animal origin, and this label-free detection method could also be applied to analyze other targets in the field of food safety. Graphical abstract.

Reproducible Molecularly Imprinted Piezoelectric Sensor for Accurate and Sensitive Detection of Ractopamine in Swine and Feed Products.

This paper describes the development of a reproducible molecularly imprinted piezoelectric sensor for the accurate and sensitive detection of ractopamine (RAC) in swine and feed products. The synthesized molecularly imprinted polymer (MIP) was directly immobilized on the surface of a quartz crystal microbalance (QCM) Au chip as the recognition element. The experimental parameters in the fabrication, measurement and regeneration process were evaluated in detail to produce an MIP-based piezoelectric sensor with high sensing capability. The developed piezoelectric sensor was verified to perform favorably in the RAC analysis of swine and feed products, with acceptable accuracy (recovery: 75.9⁻93.3%), precision [relative standard deviation (n = 3): 2.3⁻6.4%], and sensitivity [limit of detection: 0.46 ng g-1 (swine) and 0.38 ng g-1 (feed)]. This portable MIP-based chip for the piezoelectric sensing of RAC could be reused for at least 30 cycles and easily stored for a long time. These results demonstrated that the developed MIP-based piezoelectric sensor presents an accurate, sensitive and cost-effective method for the quantitative detection of RAC in complex samples. This research offers a promising strategy for the development of novel effective devices used for use in food safety analysis.

Corrigendum to "Epstein-Barr Virus-Induced Gene 3 (EBI3) Blocking Leads to Induce Antitumor Cytotoxic T Lymphocyte Response and Suppress Tumor Growth in Colorectal Cancer by Bidirectional Reciprocal-Regulation STAT3 Signaling Pathway".

[This corrects the article DOI: 10.1155/2016/3214105.].

Epstein-Barr Virus-Induced Gene 3 (EBI3) Blocking Leads to Induce Antitumor Cytotoxic T Lymphocyte Response and Suppress Tumor Growth in Colorectal Cancer by Bidirectional Reciprocal-Regulation STAT3 Signaling Pathway.

Epstein-Barr virus-induced gene 3 (EBI3) is a member of the interleukin-12 (IL-12) family structural subunit and can form a heterodimer with IL-27p28 and IL-12p35 subunit to build IL-27 and IL-35, respectively. However, IL-27 stimulates whereas IL-35 inhibits antitumor T cell responses. To date, little is known about the role of EBI3 in tumor microenvironment. In this study, firstly we assessed EBI3, IL-27p28, IL-12p35, gp130, and p-STAT3 expression with clinicopathological parameters of colorectal cancer (CRC) tissues; then we evaluated the antitumor T cell responses and tumor growth with a EBI3 blocking peptide. We found that elevated EBI3 may be associated with IL-12p35, gp130, and p-STAT3 to promote CRC progression. EBI3 blocking peptide promoted antitumor cytotoxic T lymphocyte (CTL) response by inducing Granzyme B, IFN-γ production, and p-STAT3 expression and inhibited CRC cell proliferation and tumor growth to associate with suppressing gp130 and p-STAT3 expression. Taken together, these results suggest that EBI3 may mediate a bidirectional reciprocal-regulation STAT3 signaling pathway to assist the tumor escape immune surveillance in CRC.

One stone kills three birds: novel boron-containing vesicles for potential BNCT, controlled drug release, and diagnostic imaging.

A new conjugate polymer was prepared by an efficient thiol-ene coupling of one carborane with a linear PEG chain (Mn = 2,000 g/mol), and each carborane was further labeled with a fluorescence rhodamine dye. Such a novel polymer can associate in water to form narrowly distributed spherical vesicles, which were characterized using a range of methods, including laser light scattering, confocal laser scanning microscopy, and TEM. The vesicular structure is potentially multifunctional in biomedical applications, namely, serving as a boron neutron capture therapy (BNCT) agent, a hydrophilic drug carrier, and a diagnostic imaging fluorescent probe. As expected, either cleaving the thiol-ene linked PEO chain by esterase or destroying carborane by neutron irradiation results in a dismantlement of such a vesicle structure to release its encapsulated drugs. Its potential biomedical applications have been evaluated in vitro and in vivo. Our preliminary results reveal that these small vesicles can be quickly taken up by cells and have an enhanced stability in the bloodstream so that their targeting to specific cancer cells becomes feasible.

Plasmon AgNPs/MoS2/ZnO nanorods array ternary heterojunctions enabling high-efficiency solar-light energy utilization for photocatalysis and recyclable SERS detection.

BACKGROUND: Surface-enhanced Raman scattering (SERS) has gained widespread use in molecule-level detection benefiting from its high sensitivity, nondestructive data acquisition, and capacity for providing molecular fingerprint information. However, the strong adhesion of target molecules to the substrate (known as the "memory effect") inherently hinders the reusability of SERS substrates. Research has shown that self-cleaning SERS substrates based on versatile semiconductor materials with SERS enhancement capabilities and solar photocatalytic properties offer an effective platform for the sensitive detection and degradation of harmful molecules. RESULTS: In this research, a resuable SERS-active substrate was facilely fabricated by anchoring silver nanoparticles (AgNPs) to the edges of MoS2 nanosheet decorated on ZnO nanorod arrays (NRAs). This innovative design exhibited a remarkable SERS enhancement factor (EF) of 4.6 × 107 and demonstrated significant solar photocatalytic efficiency. Such superior characteristics of ternary plasma heterojunction were ascribable to the synergistic effect of the "Schottky barrier" and "hot spots" between MoS2 and AgNPs, the inherent chemical enhancement proficiency of the MoS2/ZnO NRAs heterojunction, as well as the ultrafast electron transfer within the ternary heterojunction. SIGNIFICANCE: The developed ternary heterojunction substrate enabled highly sensitive SERS detection of trace amounts of organic molecules. Moreover, this SERS substrate exhibited self-cleaning and recyclability via solar-light-driven photocatalysis. This bifunctional recyclable SERS substrate proved capable of meeting various requirements for routine monitoring of environmental organic pollutants and provided a robust avenue for advancing energy utilization materials that serve as high-performance SERS sensors and catalysts.

Computational simulation-assisted design and experimental verification of molecularly imprinted polymers for selective extraction of chlorogenic acid.

Chlorogenic acid (CGA) is an active ingredient in honeysuckle with a broad-spectrum of antibacterial activity, suppressing tumor growth and other pharmacological effects. However, it is susceptible to damage during traditional extraction and separation processes. Therefore, developing selective and efficient extraction methods of CGA is essential. Based on computational molecular simulations, a reliable and efficient molecularly imprinted polymers (MIPs) were successfully developed for selective extraction of CGA. MIPs and non-molecularly imprinted polymers (NIPs) were synthesized using a precipitation polymerization method, employing three different functional monomers: [methacrylic acid (MAA), 4-vinylpyridine (4-VP), and methyl methacrylate (MMA)], with CGA serving as the template molecule. To simulate the polymers and predict the optimal ratio between the template and functional monomer, the computational studies and adsorption performance experiments were carried out. The adsorption characteristics and thermal stability of polymers were evaluated by isothermal adsorption, adsorption kinetics, selective adsorption and thermogravimetric analysis, aiming to obtain the MIPs with specific recognition and selectivity for CGA. When the molar ratio of template CGA to functional monomer 4-VP was 1:8, the prepared MIPs was found to have the maximum adsorption capacity (14.85 mg g-1) and the highest imprinting factor (1.74) at the CGA concentration of 100 mg L-1. These results were consistent with those obtained by computational molecular simulation. This study not only provides good guidance for developing separation materials for extracting CGA from natural plants but also inspires the application of computer simulation and molecular docking techniques in the preparation of specific MIPs materials.

Bimetallic Ag/Au nanoclusters encapsulated in ZIF-8 framework: A novel strategy for ratiometric fluorescence detection of doxycycline in food.

This study successfully encapsulated the Ag+-doped Au nanoclusters (Ag/AuNCs) within the ZIF-8 framework to construct a novel Ag/AuNCs@ZIF-8 ratiometric fluorescent probe for the antibiotic doxycycline (DOX) detection. The incorporation of Ag+ contributed to the fluorescence enhancement of the nanoclusters through the "silver effect", consequently improving the stability of the developed bimetallic Ag/AuNCs. Furthermore, the encapsulation of bimetallic Ag/AuNCs within the ZIF-8 framework restricted their intramolecular vibrations, resulting in further amplification of fluorescence intensity at 595 nm. The ZIF-8 also sensitized the restoration of DOX green fluorescence at 515 nm. Within the concentration range of 0.001-20 µg mL-1, the ratio of fluorescence intensity (F515/F595) exhibited a favorable linearity for DOX concentration, with a detection limit of 36.8 ng mL-1. This ratiometric fluorescence approach had the promising potential for accurate and efficient quantitative detection of DOX residue in food and served as a valuable reference for rapid monitoring of food contaminants.

Microfluidic paper-based analysis device applying black phosphorus nanosheets@MWCNTs-COOH: A portable and efficient strategy for detection of ß-Lactoglobulin in dairy products.

This study prepared a novel, portable and cost-effective microfluidic paper-based electrochemical analysis device (µ-PAD) using black phosphorus nanosheets@carboxylated multi-walled carbon nanotubes (BPNSs@MWCNTs-COOH) nanocomposites for ß-lactoglobulin (ß-LG) detection. At the appreciate ratio, the synthesized BPNSs@MWCNTs-COOH was demonstrated to not only serve as a high-quality substrate for the specific aptamer immobilization, but also improve the electron transfer capability of the sensing interface. The µ-PADs, utilizing BPNSs@MWCNTs-COOH and aptamer recognition, exhibited a wider detection range (10-1000 ng mL-1) and lower detection limit (LOD: 0.12 ng mL-1) for ß-LG, and demonstrated enhanced specificity, satisfactory anti-interference ability and stability. When applied to the ß-LG determination in dairy samples, the µ-PAD yielded ß-LG concentrations highly correlated with those obtained using the HPLC method (R2: 0.9982). These results emphasized the reliable performance of the developed µ-PADs in ß-LG allergen quantification, highlighting their potential as an efficient platform for the rapid screening of ß-LG allergens.