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Objective To investigate the effect of the spleen on the function of intestinal barrier during the course of acute pancreatitis in rats.Methods Rats were randomly divided into four groups: sham operation group; actue pancreatitis group; splenectomy group;splenectomy plus acute pancreatitis group.The serum levels of TNF-?,IL-1,IL-6 and IL-10 in each group were examined 24 hours after operation.Two days after operation ,the rate of bacterial translocation(BT) was determined and the terminal ileum was excised and examined by transmission electron microscopy to detect the injury of intestinal mucosa.Results The serum levels of TNF-?,IL-1?,IL-6 and IL-10 in splenectomy plus acute pancreatitis group were 3.06?3.61,16.46?5.52,19.90?6.89,6.94?3.93,and in acute pancreatitis group were 19.93?2.38, 42.79?4.31, 20.19?3.35, 39.28?12.69 respectively. The values of TNF-?, IL-1? and IL-10 were significantly lower in splenectomy plus acute pancreatitis group than those in acute pancreatitis group(P
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Objective: Recent researches suggest that tumors are organized in a hierarchy of heterogeneous cell populations and that the capability of maintaining tumor formation/growth specifically resides in a small population of cells called cancer stem cells(CSCs).CSCs are resistant to traditional cancer chemotherapy and radiation therapy because they highly express ATP-binding cassette(ABC) drug transporters and are relatively quiescent,with higher DNA repair and anti-apoptosis abilities.The purpose of this study is to investigate the relationship of ABC transporters with the multi-drug resistance and cancer stem cells of SPC-A1 cell lines.Methods: On the basis of Docetaxel-resistant cell line-SPC-A1/ Docetaxel,we compared the content and biological characteristics of SP cells,the expression of ABC transporters and its effect on the multi-resistance to Docetaxel between SPC-A1 and SPC-A1/ Docetaxel cell lines.Results: SP cells existed in both the SPC-A1 and the SPC-A1/ Docetaxel cell line,with a higher content in the latter.P-gp and BCRP obviously expressed in the SPC-A1/ Docetaxel and SPC-A1/ Docetaxel-SP cells,but only the expression of BCRP was increased in SPC-A1-SP cells.SPC-A1-SP cells also showed obviously higher abilities of proliferation,cloning and tumor formation than SPC-A1/ Docetaxel-SP cells.The multi-drug resistance of SPC-A1/ Docetaxel cells could be reversed with verapamil,but their resistance to Docetaxel could not be reversed completely with the same concentration of the drug.Conclusion: BCRP plays a major role in separating SP cells with tumor stem cell traits and its expression counts for the multi-drug resistance of SPC-A1-SP cells.