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It is believed that the basic pathogenesis of oculomotor nerve paralysis （ONP） is qi and blood depletion and channel obstruction. Taking channel pattern differentiation as the core， it is recommended to use acupuncture mainly based on location， syndrome and channel deficiency-excess differentiation. Location and channel tropism differentiation mainly follows the principle of “where the channels and collaterals pass， the main treatment can reach”， and commonly uses acupuncture points on Dumai （督脉）， the hand Shaoyang （少阳） Sanjiao （三焦） channel， the hand Taiyang （太阳） small intestine channel， three yang channels of the foot， the hand Shaoyin （少阴） heart channel， the foot Jueyin （厥阴） liver channel， and others， and combines scalp acupuncture with electro-acupuncture to enhance channel stimulation. Pattern and channel tropism differentiation is to differentiate the entered channel according to clinical symptoms and zang-fu patterns. The differentiation of deficiency-excess should be payed attention to， and the principle of “supplementing the deficiency， and draining the excess” should be followed. The supplementing and draining method should be used rationally by considering the differences in the supplementing and draining acupoints themselves， as well as the actual condition of the patients. Simultaneously， it is suggested to combine manipulation and stimulation amount and effect in clinical practice.
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Objective To analyze effect of Treadmill Training on neuropsychiatric symptoms and quality of life in elderly patients with Alzheimer′s disease. Methods From May 2018 to November 2018, the experimental design was adopted. The patients with 55-75 years old AD, the First Affiliated Hospital of Quanzhou Fujian Medical University were randomly divided into groups. Group (treadmill training, 68 patients), control group (no intervention, 68 patients). The two groups were evaluated before the training and 20 weeks after training, using the Neuropsychology Scale (NPI-Q) and the Alzheimer's disease Quality of Life Questionnaire (QOL-AD), and the t-test, two-factor repeated measures analysis of variance, etc. Statistical methods for data analysis. Results There were no significant differences in neuropsychiatric scores and QOL scores between the two groups before training (P＞0.05). After training through a 20-week treadmill, the total score of neuropsychiatric symptoms in patients with Alzheimer's disease in the experimental group (t=-1.317, P=0.204 before training; t=-10.01, P=0.046 after 20 weeks of training), affective symptoms ( Before training, t=-1.684, P=0.092; t=-11.26, P=0.043 after 20 weeks of training, psychiatric symptoms (t=1.168 before training, P=0.235; t=-9.94 after training for 20 weeks, P=0.034) ), behavioral symptoms (t=1.952 before training, P=0.129; t=-3.61, P=0.028 after 20 weeks of training), and QOL total score (t=1.452, P=0.140 before training; t=22.27 after training, P=0.040) were statistically different; and the improvement of neuropsychiatric symptoms in the experimental group was significantly different from that in the control group (F=23.10, P=0.000), while the QOL of the experimental group was also significantly different from the control group (t=- 8.88, P=0.000). Conclusion Non-pharmacological treatment with treadmill training can improve neuropsychiatric symptoms and improve quality of life in elderly AD patients.
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Objective@#To analyze effect of Treadmill Training on neuropsychiatric symptoms and quality of life in elderly patients with Alzheimer′s disease.@*Methods@#From May 2018 to November 2018, the experimental design was adopted. The patients with 55-75 years old AD, the First Affiliated Hospital of Quanzhou Fujian Medical University were randomly divided into groups. Group (treadmill training, 68 patients), control group (no intervention, 68 patients). The two groups were evaluated before the training and 20 weeks after training, using the Neuropsychology Scale (NPI-Q) and the Alzheimer's disease Quality of Life Questionnaire (QOL-AD), and the t-test, two-factor repeated measures analysis of variance, etc. Statistical methods for data analysis.@*Results@#There were no significant differences in neuropsychiatric scores and QOL scores between the two groups before training (P>0.05). After training through a 20-week treadmill, the total score of neuropsychiatric symptoms in patients with Alzheimer's disease in the experimental group (t=-1.317, P=0.204 before training; t=-10.01, P=0.046 after 20 weeks of training), affective symptoms (Before training, t=-1.684, P=0.092; t=-11.26, P=0.043 after 20 weeks of training, psychiatric symptoms (t=1.168 before training, P=0.235; t=-9.94 after training for 20 weeks, P=0.034)), behavioral symptoms (t=1.952 before training, P=0.129; t=-3.61, P=0.028 after 20 weeks of training), and QOL total score (t=1.452, P=0.140 before training; t=22.27 after training, P=0.040) were statistically different; and the improvement of neuropsychiatric symptoms in the experimental group was significantly different from that in the control group (F=23.10, P=0.000), while the QOL of the experimental group was also significantly different from the control group (t=- 8.88, P=0.000).@*Conclusion@#Non-pharmacological treatment with treadmill training can improve neuropsychiatric symptoms and improve quality of life in elderly AD patients.
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Objective To observe the effect and adverse reaction of fentanyl ransdermal system on the cancer pain resulted from lung cancer metastasizing to bone.Methods Thirty -two patients with moderate to severe cancer pain of bone metastasis from lung cancer were treated with fentanyl transdermal system.Analgesic effect was observed.The quality of life and adverse reactions were evaluated.Results The PI evaluation were (7.62 ± 1.35)and (1.93 ±2.41)before /after treated with fentanyl ransdermal system.The differences of bone pain between before and after treated were significant(t =3.983,P <0.01).Among the thirty -two patients with moderate to severe cancer pain used by fentanyl transdermal system,complete relief was in 12 cases(37.5%),apparent relief was in 11 cases(34.3%),moderate relief was in 7 cases(21.8%),mild relief was in 2 cases(6.3%),the total relief rate was 93.7%.The adverse reactions included hypersomnia were in 13 cases,dizziness in 11 cases,nausea and vomiting in 6 cases,constipation in 5 cases,pruritus and erythra in 4 cases,dysuria in 2 cases.All above adverse reactions dis-appeared after stopping fentanyl ransdermal system and /or undergoing symptomatic treatment.Conclusion Fentanyl transdermal system is easy to use,the effect is significant,there is few adverse reactions,which can significantly improve quality of life of cancer patients.
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Objective To observe apoptosis of primary cultured synoviocytes from patients with rheumtoid arthritis (RA) which induced by transfected recombinant plasmid carring Fas ligand (FasL) gene, aimed to detect the aim targets and develope gene therapy for RA by intra-articular injection. Methods FasL cDNA was introducted into the plasmid pcDNA3.1-neo by reverse transcriptase-polymerse chain reaction (RT-PCR) to set up the recombinated human FasL gene expression carrier, Then the pcDNA3.1-FasL was transducted into the primary cultured synovial cells by catholyte liposomes. After screened by G418, transgenic synovial cells were choosen. Finally through the microscope, TUNEL, Annexin Ⅴ-FITC and PI staining with flow cytometry, we observed the apoptosis state of each synovial cell. Results Prokaryotic and eukaryon carrier with FasL gene were established successfilly. The gene sequencing had completely coincident with the lierature. When eukaryon carrier with FasL (pcDNA3.1-FasL) was transducted into the index number livinging RA synovial cells after 15 h, most synovial cells presented distortion transformation, and the minority sheded from the bottom wall. The transgenic RA synovial cells after screened by G418, only a little amount survived about 1~2 per sight, and the cells grew slowly, the normal matched cells were cloned after 2~4 weeks. The FasL gene transgenic RA synovial cells presented positive findings by TUNEL staining. Early apoptosis cells by Annexin Ⅴ/PI staining, but the difference wasn′t significant. Conclusion The eukaryon carrier with FasL (pcDNA3.1-FasL) can be transferred into RA synovial cells. It can induce synovial cell apoptosis.
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AIM: To investigate the correlation between the signal pathway of IKK/NF-?B and the anti-oxidant activity in asthmatic rats and the modulation of Ginkgo biloba extract (Egb). METHODS: Thirty-six male SD rats were randomly divided into three groups: control group (group C), asthmatic group (group A) and Egb group(group E). Asthma in rats was established by ovalbumin (OVA) challenge methods. The mRNA of IKK? and the protein of NF-?B P65 in lung tissue were assessed by using in situ hybridization with oligonucleotide probe and immunohistochemisty, respectively. RESULTS: The expression of IKK? mRNA and NF-?B P65 protein in group A were significantly increased when compared with group C (P
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OBJECTIVE: We used the SD rat's bone marrow stromal cells (BMSCs) cultured in vitro to observe the effects of Bugu Mixture on the apoptosis and to explore the molecular biologic mechanism of the treatment of osteoporosis with Bugu Mixture. METHODS: BMSCs were separated from the bones of the extremities of SD rats in vitro. The morphologic changes, the apoptosis cell cycles, the mitochondrion membrane potential changes, and the Bcl-2 and Bax gene expression were observed, and the effects of Bugu Mixture on the course of cell apoptosis were evaluated. RESULTS: The earlier use of Bugu Mixture could decrease the cells blocked in G0/G1 phase, and promote their synthesis of DNA in S phase. The expression of Bcl-2 was higher in the Bugu Mixture group than that in the all-trans retinoic acid (ATRA) induced group, and the expression of Bax was lower in the Bugu Mixture group than that in the ATRA induced group. The mitochondrion membrane potential descended significantly in the Bugu Mixture group than that in the ATRA induced group. CONCLUSION: The mechanism of the treatment of osteoporosis with Bugu Mixture is that the earlier use of Bugu Mixture can decrease the amount of apoptostic cells induced by ATRA, thus promoting the cell mitosis and restraining the apoptosis. It can also act as a protector to Bcl-2 located on the mitochondrion membrane. By preventing the transferring of the Bax protein from cell-plasma to mitochondrion membrane, it takes the advantage of Bcl-2 in forming Bcl-2/Bax homodimer so as to prevent the opening of the permeability transition pore to avoid the changing of mitochondrion membrane potential and the destruction of biosynthesis caused by the mitochondrion release of apoptosis inducing factors and to reach the objective of restraining apoptosis.