Omni- and unidirectional data unit photolithography for high similarity and multiple angular segment display-based encoded microparticle fabrication.

In this study, we propose high similarity and multiple angular segment display-based encoded microparticle fabrication using omni- and unidirectional data unit photolithography systems. Uniform high-correlation values are obtained regardless of the decoding direction when an omnidirectional data unit is used. In addition, multiple display angles are analyzed based on unidirectional data units with varying slit widths. Finally, encoded microparticles for multiple angular segment displays are fabricated and selective information recognition is demonstrated.

Four-Dimensional Physical Unclonable Functions and Cryptographic Applications Based on Time-Varying Chaotic Phosphorescent Patterns.

Physical unclonable functions (PUFs) have attracted interest in demonstrating authentication and cryptographic processes for Internet of Things (IoT) devices. We demonstrated four-dimensional PUFs (4D PUFs) to realize time-varying chaotic phosphorescent randomness on MoS2 atomic seeds. By forming hybrid states involving more than one emitter with distinct lifetimes in 4D PUFs, irregular lifetime distribution throughout patterns functions as a time-varying disorder that is impossible to replicate. Moreover, we established a bit extraction process incorporating multiple 64 bit-stream challenges and experimentally obtained physical features of 4D PUFs, producing countless random 896 bit-stream responses. Furthermore, the weak and strong PUF models were conceptualized and demonstrated based on 4D PUFs, exhibiting superior cryptological performances, including randomness, uniqueness, degree of freedom, and independent bit ratio. Finally, the data encryption and decryption in pictures were performed by a single 4D PUF. Therefore, 4D PUFs could enhance the counterfeiting deterrent of existing optical PUFs and be used as an anticounterfeiting security strategy for advanced authentication and cryptographic processes of IoT devices.

All-in-one platform: Versatile, Easy, and User-friendly System (VEUS) based on automated and expert-independent antibody immobilization and immunoassay by utilizing customized movement of magnetic particles.

The ELISA is the most worldwide method for immunoassay. However, the ELISA is losing ground due to low reproducibility of manual experimental processes in both R&D and IVD areas. An automated platform is a good solution, but there are still limitations owning to extremely high cost and requiring large space to set up especially for a small size laboratory. Here, we present a novel all-in-one platform called "VEUS" settable on the laboratory table that offers comprehensive automation of the entire multiplex immunoassay process by exploiting antibody conjugated magnetic particles, quality control and then immunoanalytical reaction, thereby enhancing detection sensitivity and high reproducibility. As a proof of concept, the system exhibits a sensitive LOD of 0.6 and 3.1 pg mL-1 within 1 h run, comparable precision that of molecular diagnostic systems based on PCR method, enabling rapid multiplex diagnosis of Influenza A, Influenza B, and COVID-19 viruses with similar symptoms. Through automation by the all-in-one system, it can be used by novice users, something innovative for immunoassays, relying heavily on user experience. Furthermore, it can contribute to streamline entire immunoassay processes of diverse biomarkers with high reproducibility and convenience in laboratories.

Chaotic Organic Crystal Phosphorescent Patterns for Physical Unclonable Functions.

Since the 4th Industrial Revolution, Internet of Things based environments have been widely used in various fields ranging from mobile to medical devices. Simultaneously, information leakage and hacking risks have also increased significantly, and secure authentication and security systems are constantly required. Physical unclonable functions (PUF) are in the spotlight as an alternative. Chaotic phosphorescent patterns are developed based on an organic crystal and atomic seed heterostructure for security labels with PUFs. Phosphorescent organic crystal patterns are formed on MoS2 . They seem similar on a macroscopic scale, whereas each organic crystal exhibits highly disorder features on the microscopic scale. In image analysis, an encoding capacity as a single PUF domain achieves more than 1017 on a MoS2 small fragment with lengths of 25 µm. Therefore, security labels with phosphorescent PUFs can offer superior randomness and no-cloning codes, possibly becoming a promising security strategy for authentication processes.

Gradient-Wrinkled Microparticle with Grayscale Lithography Controlling the Cross-Linking Densities for High Security Level Anti-Counterfeiting Strategies.

Physical unclonable functions (PUFs) enable different characteristics according to the purpose, such as easy to access identification, high security level, and high code capacity, against counterfeiting a product. However, most multiplex approaches have been implemented by embedding several security features rather than one feature. In this paper, we present a high security level anti-counterfeiting strategy using only labyrinth wrinkle patterns with different complexities, which can be used as unique and unclonable codes. To generate codes with different levels in a microtaggant, we fabricated wrinkle patterns with characteristic wavelength gradients using grayscale lithography. The elastic modulus of the polymer substrate and corresponding wavelength after the wrinkling process were controlled by designing the gray level of each subcode region in a gray-level mask image for photopolymerization of the microparticle substrate. We then verified the uniqueness of the extracted minutia codes through a cross-correlation analysis. Finally, we demonstrated the authentication strategies by decoding different minutia codes according to the scanning resolution during the decoding. Overall, the presented patterning method can be widely used in security code generation.

Bacterial Isolation Microwell-Plug (µWELLplug) for Rapid Antibiotic Susceptibility Testing Using Morphology Analysis.

The rapid and accurate diagnosis of infectious diseases with high morbidity rates is crucial because it can minimize the misuse and overuse of antibiotics and increase survival rates in dreadful conditions. The conventional antibiotic susceptibility test (AST) systems used to choose appropriate antibiotics require long wait times to obtain results and cannot prevent the misuse or overuse of antibiotics by clinicians who need to quickly treat patients and cannot wait to identify the underlying cause of their symptoms. Therefore, several rapid AST (rAST) methods have been developed to provide quick test results, but they are complicated to operate, require additional equipment or materials, and give less accurate results than the conventional AST methods. In this study, we propose an rAST method that can obtain precise outcomes from a simple process with a short running time using a bacterial isolation microwell-plug (µWELLplug) in a conventional 96-well plate. The specifically designed hydrogel component of the µWELLplug provides a simple process for cell isolation and the observation of bacterial growth and morphological changes induced by a variety of antibiotic concentrations. The µWELLplug is placed over each well of the 96-well plate, and then bacterial or eukaryotic cells are isolated in the microwells and treated with different antibiotic concentrations to observe their effects. Saccharomyces cerevisiae (yeast, eukaryote), Streptomyces atratus (actinomycetes, prokaryote), Escherichia coli, Staphylococcus aureus, and methicillin-resistant S. aureus were cultivated and tested using the µWELLplug. The minimum inhibitory concentration values from this system were obtained in 3-4 h and correlated well with those from the conventional AST methods whose running time is 18-24 h.

Self-organization of maze-like structures via guided wrinkling.

Sophisticated three-dimensional (3D) structures found in nature are self-organized by bottom-up natural processes. To artificially construct these complex systems, various bottom-up fabrication methods, designed to transform 2D structures into 3D structures, have been developed as alternatives to conventional top-down lithography processes. We present a different self-organization approach, where we construct microstructures with periodic and ordered, but with random architecture, like mazes. For this purpose, we transformed planar surfaces using wrinkling to directly use randomly generated ridges as maze walls. Highly regular maze structures, consisting of several tessellations with customized designs, were fabricated by precisely controlling wrinkling with the ridge-guiding structure, analogous to the creases in origami. The method presented here could have widespread applications in various material systems with multiple length scales.