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Chemoenzymatic epoxidation of olefin mediated by lipase is a green and environmentally friendly alternative process. However, the mass transfer barrier and lipase deactivation caused by the traditional organic-water biphasic reaction system have always been the focus of researchers' attention. To overcome these issues, we investigated the effects of reaction temperature and two important substrates (H2O2 and acyl donor) on the epoxidation reaction and interfacial mass transfer. As a result, we determined the optimal reaction conditions: a temperature of 30 °C, 30 wt-% H2O2 as the oxygen source, and 1 M lauric acid as the oxygen carrier. Additionally, by simulating the conditions of shaking flask reactions, we designed a batch reactor and added a metal mesh to effectively block the direct contact between high-concentration hydrogen peroxide and the enzyme. Under these optimal conditions, the epoxidation reaction was carried out for 5 h, and the product yield reached a maximum of 93.2%. Furthermore, after seven repetitive experiments, the lipase still maintained a relative activity of 51.2%.
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Peróxido de Hidrógeno , Lipasa , Monoterpenos Bicíclicos , AlquenosRESUMEN
An enzyme-catalyzed fluorescence "switch" type sensor was constructed for the determination of alkaline phosphatase (ALP) activity by combining the fluorescence quenching effect of Ag+ on ultrathin g-C3N4 nanosheets (CNNSs) with the simple redox reaction of AA and Ag+. Briefly, Ag+ exhibits a significant quenching effect on the fluorescence of CNNSs. Thus the fluorescence signal of the CNNS-Ag+ system is extremely weak even in the presence of l-ascorbic acid-2-phosphate (AAP) ("off" state). When ALP coexists in the system, the enzyme can specifically catalyze the hydrolysis of AAP to form ascorbic acid (AA), which reduces Ag+ to Ag0. In this case, the fluorescence signal of the system is recovered ("on" state). Based on this principle, a signal-enhanced CNNS fluorescence sensor was developed to determine the activity of alkaline phosphatase. The experimental results show that the detection range of alkaline phosphatase is 0.5-20 U L-1, and the detection limit is 0.05 U L-1 (S/N = 3). Meanwhile, this method was used to assay ALP in serum samples.
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Fosfatasa Alcalina , Técnicas Biosensibles , Catálisis , NitrilosRESUMEN
Pyrophosphate anion (P2 O7 4- , PPi) is considered as a potential biomarker for arthritic diseases because high levels of PPi may result in calcium pyrophosphate dehydrate crystal deposition diseases. In this study, a simple fluorescence method for PPi was demonstrated by organic integration of the efficient fluorescence quenching ability of copper ions to DNA-scaffolded silver nanoclusters and the strong affinity of PPi towards copper ions. This simple fluorescence sensor showed a low detection limit (0.28 µM based on signal/noise = 3) towards the detection of PPi. Practical application of this method was also validated by detection of PPi in the synovial fluid.
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ADN/química , Difosfatos/análisis , Colorantes Fluorescentes/química , Nanopartículas del Metal/química , Plata/química , Iones/análisis , Espectrometría de FluorescenciaRESUMEN
Water-dispersed silicon quantum dots (SiQDs) with the quantum yield of 25% was prepared using aminopropyltrimethoxysilane as the silicon source and ascorbic acid (AA) as the reduction reagent. The SiQDs display blue fluorescence with excitation/emission peaks at 350 nm/440 nm. The synthesized SiQDs are shown to be a viable "on-off-on" fluorescent probe for the detection of Cr(VI) and AA. Cr(VI) ions exert an inner filter effect on the fluorescence of the SiQDs which results in a reduction of fluorescence (off-state). On addition of AA, Cr(VI) is chemically reduced to Cr(III) which weakens the inner filter effect and restores fluorescence (on-state). The method has low detection limits for both Cr(VI) and AA (0.16 µM and 0.57 µM, respectively). It was applied to the analysis of spiked lotus seeds and human serum samples. Graphical abstract A simple and facile "on-off-on" fluorometric method for Cr(VI) and ascorbic acid (AA) was developed using water-soluble silicon quantum dots (SiQDs) as the fluorescent probe. The approach was also used to assay Cr(VI) and AA in the lotus seeds and human serum, respectively.
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A simple and sensitive analytical method for four isomers of glycopyrrolate in rat plasma was developed using cation-selective exhaustive injection-sweeping cyclodextrin-modified electrokinetic chromatography (CSEI-Sweeping-CDEKC) for online enrichment combined with dispersive micro-solid-phase extraction pretreatment. The CSEI-Sweeping-CDEKC was conducted on an uncoated fused silica capillary (40.2 cm × 75 µm) with an applied voltage of -20 kV. The electrophoretic analysis was carried out in 30 mM phosphate solution at pH 2.0 containing 20 mg/mL sulfated-ß-cyclodextrin and 5% acetonitrile. Under these optimized conditions, the detection limit for racemic glycopyrrolate was found to be 2.0 ng/mL and this method could increase 495-fold detection sensitivity compared with the traditional injection method. Additionally, the parameters that affected the extraction efficiency of dispersive micro-solid-phase extraction were also examined systematically. The glycopyrrolate isomers in rat plasma samples as low as 0.0625 µg/mL were able to be separated and detected by capillary electrophoresis with the aid of CSEI-sweeping. The findings of this study show that the dispersive micro-solid-phase extraction pretreatment coupled with CSEI-Sweeping-CDEKC is a rapid and convenient method for analyzing glycopyrrolate isomers in rat plasma.
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Glicopirrolato/sangre , Internet , Microextracción en Fase Sólida , Animales , Electroforesis Capilar , Glicopirrolato/química , Concentración de Iones de Hidrógeno , Ratas , EstereoisomerismoRESUMEN
OBJECTIVE: To study the effect of Shexiang Tongxin Dropping Pill (STDP) on angiogenesis in diabetic cardiomyopathy mice with coronary microcirculation dysfunction (CMD). METHODS: According to a random number table, 6 of 36 SPF male C57BL/6 mice were randomly selected as the control group, and the remaining 30 mice were injected with streptozotocin intraperitoneally to replicate the type 1 diabetes model. Mice successfully copied the diabetes model were randomly divided into the model group, STDP low-dose group [15 mg/(kg·d)], medium-dose group [30 mg/(kg·d)], high-dose group [60 mg/(kg·d)], and nicorandil group [15 mg/(kg·d)], 6 in each group. The drug was given by continuous gavage for 12 weeks. The cardiac function of mice in each group was detected at the end of the experiment, and coronary flow reserve (CFR) was detected by chest Doppler technique. Pathological changes of myocardium were observed by hematoxylin-eosin staining, collagen fiber deposition was detected by masson staining, the number of myocardial capillaries was detected by platelet endothelial cell adhesion molecule-1 staining, and the degree of myocardial hypertrophy was detected by wheat germ agglutinin staining. The expression of the vascular endothlial growth factor (VEGF)/endothelial nitric oxide synthase (eNOS) signaling pathway-related proteins in myocardial tissue was detected by Western blot. RESULTS: Compared with the model group, medium- and high-dose STDP significantly increased the left ventricular ejection fraction and left ventricular fraction shortening (P<0.01), obviously repaired the disordered cardiac muscle structure, reduced myocardial fibrosis, reduced myocardial cell area, increased capillary density, and increased CFR level (all P<0.01). Western blot showed that high-dose STDP could significantly increase the expression of VEGF and promote the phosphorylation of vascular endothelial growth factor receptor 2, phosphoinositide 3-kinase, protein kinase B, and eNOS (P<0.05 or P<0.01). CONCLUSION: STDP has a definite therapeutic effect on diabetic CMD, and its mechanism may be related to promoting angiogenesis through the VEGF/eNOS signaling pathway.
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In this study, a simple and reproducible method for enantioseparation and determination of dl-tryptophan (DL-Trp) was developed by using a partial filling technique in combination with MEKC. The corresponding L-Trp specific DNA aptamer was used as a chiral selector. Sodium cholate was used to form the chiral micelles and to enhance the enantioseparation of the enantiomers. Effects of aptamer concentration, filling time, buffer composition, and separation voltage on the enantioseparation were evaluated. The Mg(2+) and Na(+) concentration in separation buffer was found to effectively affect the separation efficiency and reproducibility. Under the optimal conditions, D- and L-Trp were completely enantioseparated in less than 9 min. This aptamer-based partial-filling approach has the potential to be extended to the separation of other enantiomers after the replacement of corresponding specific aptamers.
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Aptámeros de Nucleótidos/química , Cromatografía Capilar Electrocinética Micelar/métodos , Triptófano/análisis , Triptófano/química , Cationes/química , Magnesio/química , Análisis de Regresión , Reproducibilidad de los Resultados , Sodio/química , EstereoisomerismoRESUMEN
Interferon-regulatory factor 5 (IRF5) participates in the regulation of apoptosis, affects the phenotype of inflammatory macrophages and plays an essential role in the inflammatory response. However, the role of IRF5 in the progression of amyotrophic lateral sclerosis (ALS) remains largely unknown. Here, we show that IRF5 mainly accumulated in the nucleus in cells expressing the truncated 25 kD C-terminal fragments of TDP-43 (TDP-25, named TDP-25 cells hereafter). IRF5 knockdown using a lentivirus carrying an shRNA in TDP-25 cells exerted a protective effect and reduced the level of the apoptosis-related protein cleaved caspase-9 and the cell cycle arrest protein p21, while increasing the expression of the antioxidant transcription factor nuclear factor erythroid 2-related factor 2 (Nrf2) and its target molecule glutamate-cysteine ligase modulatory subunit (GCLM). Furthermore, IRF5-knockdown cells showed improved mitochondrial swelling and cristae dilation. In addition, we found that IRF5 mediated neuronal injury partly through the negative regulation of TANK-binding kinase 1 (TBK1). These data indicate that the loss of IRF5 in TDP-25 cells exerts a protective effect mainly by inhibiting apoptosis, regulating cell cycle arrest and alleviating oxidative stress.
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Esclerosis Amiotrófica Lateral , Humanos , Esclerosis Amiotrófica Lateral/metabolismo , Proteínas de Unión al ADN/metabolismo , Factores Reguladores del Interferón/genética , Factores Reguladores del Interferón/metabolismo , Estrés Oxidativo/fisiología , Proteínas de Ciclo Celular/metabolismo , Fenotipo , Proteínas Serina-Treonina Quinasas/genética , Fragmentos de Péptidos/metabolismoRESUMEN
Transverse aortic constriction (TAC) is a frequently used model to investigate pressure overload-induced progressive heart failure (HF); however, there is considerable phenotypic variation among different mouse strains and even sub-strains. Moreover, less is known about the TAC model in ICR mice. Therefore, to determine the suitability of the ICR strain for TAC-induced HF research, we compared the effects of TAC on ICR and C57BL/6J mice at one, two and four weeks post-TAC via echocardiography, organ index, morphology, and histology. At the end of the study, behavior and gene expression patterns were assessed, and overall survival was monitored. Compared to the sham-operated mice, ICR and C57BL/6J mice displayed hypertrophic phenotypes with a significant increase in ventricle wall thickness, heart weight and ratio, and cross-sectional area of cardiomyocytes after a 2-week TAC exposure. In addition, ICR mice developed reduced systolic function and severe lung congestion 4 weeks post-TAC, whereas C57BL/6J did not. Besides, ICR mice demonstrated comparable survival, similar gene expression alteration but severer fibrotic remodeling and poor behavioral performance compared to the C57BL/6J mice. Our data demonstrated that ICR was quite sensitive to TAC-induced heart failure and can be an ideal research tool to investigate mechanisms and drug intervention for pressure overload-induced HF.
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A new method for separation and determination of amygdalin and its epimer (neoamygdalin) in the epimerization of amygdalin by MEEKC is proposed. For the chiral separation of amygdalin and neoamygdalin, a running buffer composed of 80 mM sodium cholate, 5.0% v/v butan-1-ol, 0.5% v/v heptane and 94.5% v/v 30 mM Na(2) B(4) O(7) buffer (pH 9.00) is proposed. Under optimum conditions, the basic separation of amygdalin and neoamygdalin can be achieved within 7 min. The calibration curve for amygdalin showed excellent linearity in the concentration range of 20-1000 µg/mL with a detection limit of 5.0 µg/mL (S/N=3). The epimerization rate constant of amygdalin in basic microemulsion was first determined by monitoring the concentration changes of amygdalin, and the epimerization rate constant of amygdalin was found to be 2×10(-3) min(-1) at 25°C under the above optimum microemulsion conditions.
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Amigdalina/química , Electrocromatografía Capilar/métodos , Amigdalina/análisis , Tampones (Química) , Emulsiones/química , CinéticaRESUMEN
In order to improve the sensitivity of CE-ESI-MS for the analysis of brain-gut peptides, a solid-phase extraction combined with field-amplified sample injection was used for the pre-concentration of the brain-gut peptides. Compared with the conventional pressure injection method, the sensitivity in the detection of brain-gut peptides was improved more than 100-fold. The possible factors affecting sample stacking, such as the sample matrix, the composition and the length of the water column, the types and the volumes of eluent, have been investigated in detail. Under the optimum conditions, the detectable concentration of brain-gut peptides was found to be as low as 0.02 µM. A linear response concentration for the detection was developed in the range of 0.08-25 µmol/L. A real sample of human cerebrospinal fluids, which was spiked with brain-gut peptides, was also examined in order to evaluate the reliability of the proposed approach. The recovery of the method was in a range from 69.2 to 85.4%. The method was found to be reliable, accurate and potentially applicable for clinical drug analysis.
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Electroforesis Capilar/métodos , Neurotensina/análisis , Extracción en Fase Sólida/métodos , Espectrometría de Masa por Ionización de Electrospray/métodos , Tetragastrina/análisis , Humanos , Límite de Detección , Neurotensina/líquido cefalorraquídeo , Fragmentos de Péptidos , Análisis de Regresión , Reproducibilidad de los Resultados , Tetragastrina/líquido cefalorraquídeoRESUMEN
A novel, simple and sensitive method for the enantioseparation and determination of DL-tetrahydropalmatine (DL-THP) was developed using ACE in combination with partial filling technique and field-amplified sample injection. A chiral selector, i.e. BSA, was used for the enantioseparation of DL-THP in ACE. Effects of BSA concentration, pH and separation voltage on the effectiveness of the enantiomer separation were evaluated. In an optimal condition, D- and L-THP were completely enantio-separated in less than 9 min by partially filling an electrophoretic capillary with 50 micromol/L BSA (50 mbar, 100 s) and carrying out an electrophoresis with 20 mmol/L phosphate buffer (pH 7.4) at 15 kV. The sensitivity was further improved by making use of field-amplified sample injection to lower the LOD (defined as S/N=3) down to 6 ng/mL. Real samples were also tested and promising results for the determination of DL-THP enantiomers were obtained.
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Alcaloides de Berberina/química , Electroforesis Capilar/métodos , Animales , Alcaloides de Berberina/análisis , Tampones (Química) , Bovinos , Modelos Lineales , Fosfatos/química , Albúmina Sérica Bovina/química , EstereoisomerismoRESUMEN
In this article, an approach has been developed for the analysis of some small peptides with similar pI values by CE-ESI-MS based on the online concentration strategy of dynamic pH junction. The factors affected on the separation, detection and online enrichment, such as BGE, injection pressure, sheath flow liquid and separation voltage have been investigated in detail. Under the optimum conditions, i.e. using 0.5 mol/L formic acid (pH 2.15) as the BGE, preparing the sample in 50 mM ammonium acetate solution (pH 7.5), 50 mbar of injection pressure for 300 s, using 7.5 mM of acetic acid in methanol-water (80% v/v) solution as the sheath flow liquid and 20 kV as the separation voltage, four peptides with similar pI values, such as L-Ala-L-Ala (pI = 5.57), L-Leu-D-Leu (pI = 5.52), Gly-D-Phe (pI = 5.52) and Gly-Gly-L-Leu (pI = 5.52) achieved baseline separation within 18.3 min with detection limits in the range of 0.2-2.0 nmol/L. RSDs of peak migration time and peak area were in the range of 1.45-3.57 and 4.93-6.32%, respectively. This method has been applied to the analysis of the four peptides in the spiked urine sample with satisfactory results.
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Electroforesis Capilar/métodos , Oligopéptidos/orina , Espectrometría de Masa por Ionización de Electrospray/métodos , Humanos , Concentración de Iones de Hidrógeno , Punto Isoeléctrico , Modelos Lineales , Masculino , Reproducibilidad de los Resultados , Sensibilidad y EspecificidadRESUMEN
As a typical kind of bioactive flavonoid glycoside, rutin and its aglycone quercetin possess similar chemical structures and properties. It still remains a challenge to achieve reliably and accurately detection of rutin in the presence of quercetin. In this work, a simple fluorescent method combining water-dispersed silicon nanoparticles (SiNPs) with bovine serum albumin (BSA) were constructed for the selective detection of rutin in the presence of quercetin and other common compounds in traditional Chinese herbs. SiNPs with high fluorescent quantum yield and good thermostability were prepared by one-pot hydrothermal method using ferulic acid as the reduction reagent for the first time. The fluorescence of SiNPs could be obviously quenched both by rutin and quercetin in phosphate buffer solution. Interestingly, when the solution contained certain concentration of BSA, the fluorescence of the SiNPs can only be remarkably quenched by rutin. The innovative use of BSA to block the interference of quercetin make it possible to selectively detect of rutin by fluorescence spectrometry under the coexistence of quercetin. Under the optimum conditions, the fluorescence displayed a linear decrease response as the rutin concentration increased in the range of 0.33-33.30 µM with a detection limit of 0.04 µM (S/N = 3). The possible quenching mechanism of rutin to SiNPs has also explored and concluded to be mainly caused by inner filter effect. This work provides a novel methodology for the simple, low-cost and selective determination method for rutin.
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Nanopartículas , Albúmina Sérica Bovina , Quercetina , Rutina , SilicioRESUMEN
New methods based on MEEKC coupling with field-amplified sample injection (FASI) induced by ACN were proposed for five isoquinoline alkaloids (berberine, palmatine, jatrorrhizine, sinomenine and homoharringtonine) in no salt and high salt sample solution (HS). For the separation of five isoquinoline alkaloids, a running buffer composed of 18 mM sodium cholate, 2.4% v/v butan-1-ol, 0.6% v/v ethyl acetate, 10% v/v (or 30% v/v) methanol and 87.0% v/v (or 67% v/v) 5 mM Na2B4O7~10 mM NaH2PO4 buffer (pH 7.5) was developed. In order to improve the sensitivity, FASI induced by ACN was applied to increase the detection sensitivity. The detection limit was found to be as low as 0.0002 microg/mL in no salt sample solution and 0.062 microg/mL in HS. The method has been applied for the analysis of human urine spiked with analytes, and the assay results were proved to be satisfactory, and also the determination of berberine in urine sample after oral administration berberine.
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Acetonitrilos/química , Alcaloides/orina , Cromatografía Capilar Electrocinética Micelar/métodos , 1-Butanol/química , Acetatos/química , Alcaloides de Berberina/orina , Harringtoninas/orina , Homoharringtonina , Humanos , Concentración de Iones de Hidrógeno , Metanol/química , Morfinanos/orina , Reproducibilidad de los Resultados , Sensibilidad y Especificidad , Dodecil Sulfato de Sodio/química , Agua/químicaRESUMEN
In this study, approaches to improve chiral resolutions in simultaneous enantioseparation of beta-agonists by CE via a CD inclusion complexation modified with ionic liquids (ILs) are described. Different types of ILs, including tetraalkylammonium-based ILs, alkylimidazolium-based ILs and alkylpyridinium-based ILs, were examined and compared for controlling the EOF in order to improve resolutions of beta-agonists enantiomers. In this regard, tetraalkylammonium-based ILs were more effective because they could be used at much higher concentrations than other types of ILs. N-octylpyridinium hexafluorophosphate gave poor resolutions of beta-agonists enantiomers. In addition, when different ILs were mixed to use, they would present particular properties of their own. Moreover, the presence of ILs was essential in the chiral separations of (+/-) salbutamol, (+/-) cimaterol and (+/-) formoterol, which were reportedly not enantioseparated by using the buffer electrolytes containing only beta-CD as a chiral selector.
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Agonistas Adrenérgicos beta/aislamiento & purificación , Electroforesis Capilar/métodos , Líquidos Iónicos/química , beta-Ciclodextrinas/agonistas , Albuterol/aislamiento & purificación , Clenbuterol/aislamiento & purificación , Imidazoles/química , Compuestos de Piridinio/química , Compuestos de Amonio Cuaternario/química , Terbutalina/aislamiento & purificación , beta-Ciclodextrinas/aislamiento & purificaciónRESUMEN
A new rapid and reproducible method using microemulsion electrokinetic chromatography (MEEKC) combining field amplified sample injection and electroosmotic flow suppressant for the analysis of five quinolizidine alkaloids is developed in this paper. For the separation of five quinolizidine alkaloids, a running buffer composed of 1.2% (v/v) 1-butanol, 0.6% (v/v) ethyl acetate and 98.2% (v/v) 1 mM Na(2)B(4)O(7)-2 mM NaH(2)PO(4) buffer solution containing 21 mM sodium cholate (SC) (pH 6.5) was developed. The resolution of the analytes was improved significantly by adding a divalent cation (e.g., Mg(2+)) to the running buffer as an electroosmotic flow modification. In order to analyze trace quinolizidine alkaloids in traditional Chinese herbal medicines, field amplified sample injection (FASI) was applied to increase the detection sensitivity. The detection limits (defined as S/N=3) for the analytes could be as low as 0.0001 microg/mL. This method was applied for the determination of quinolizidine alkaloids in real samples with simple extraction procedures, and the assay results were satisfactory.
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Alcaloides/análisis , Cromatografía Capilar Electrocinética Micelar/métodos , Electroósmosis/métodos , Quinolizidinas/análisis , Alcaloides/química , Estructura Molecular , Quinolizidinas/química , Reproducibilidad de los ResultadosRESUMEN
ETHNOPHARMACOLOGICAL RELEVANCE: Pien-Tze-Huang (PZH) is a famous formula of traditional Chinese medicine used to treating stroke. However, the protective effect of PZH and its mechanisms in acute ischemic stroke remain to be explored. AIM OF THE STUDY: To investigate the protective effect of PZH on neuronal apoptosis in acute cerebral ischemic injury rats and explore its underlying mechanisms. MATERIALS AND METHODS: The effects of PZH were studied in acute ischemic stroke rats induced by transient middle cerebral artery occlusion, and the mitochondria-mediated apoptotic proteins including cytochrome C (Cyt C), Bax, Bcl-xl, P53, caspase-3, and caspase-9 as well as AKT and glycogen synthase kinase-3 beta (GSK-3ß) were assessed. RESULTS: Four days of PZH treatment (180â¯mg/kg) could significantly reduce cerebral infarct volume, improve neurological deficit, attenuate inflammatory response, and inhibit neuronal apoptosis in acute ischemic stroke rats. Moreover, PZH treatment significantly decreased cytosolic Cyt C, Bax, P53, cleaved caspase-3, and cleaved caspase-9 levels, but elevated mitochondrial Cyt C and Bcl-xl levels. PZH treatment also increased phosphorylation of AKT and GSK-3ß. CONCLUSION: PZH potently protects the brain from cerebral ischemia/reperfusion injury in vivo, and inhibiting mitochondria-mediated neuronal apoptosis as well as attenuating inflammatory responses may be involved in this effect. This study provides experimental basis of PZH in treating acute cerebral ischemic stroke, which would provide some novel insights for its prevention and treatment of ischemic stroke.
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Apoptosis/efectos de los fármacos , Isquemia Encefálica/prevención & control , Medicamentos Herbarios Chinos/uso terapéutico , Inhibición Neural/efectos de los fármacos , Fármacos Neuroprotectores/uso terapéutico , Accidente Cerebrovascular/tratamiento farmacológico , Animales , Apoptosis/fisiología , Isquemia Encefálica/patología , Relación Dosis-Respuesta a Droga , Medicamentos Herbarios Chinos/farmacología , Masculino , Inhibición Neural/fisiología , Fármacos Neuroprotectores/farmacología , Ratas , Ratas Sprague-Dawley , Accidente Cerebrovascular/patologíaRESUMEN
The kinetics of keto-enol tautomerism of p-hydroxyphenylpyruvic acid (pHPP) as a model of alpha-carbonyl compounds in aqueous solution at room temperature (25 degrees C) was first investigated by capillary electrophoresis with UV detection at 200 nm. The two tautomers could be separated and detected within 3 min. Since the ketonization of enolic pHPP varied with the buffer composition and buffer pH, the kinetics of pHPP was studied under different conditions, and relevant distributing fractions of enolic pHPP, ketonization rate constants and half-life were determined. In addition, beta-CD played an important part in the separation of the two tautomers, thus, the interaction between pHPP and beta-CD was also investigated by electrochemical techniques.
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Electroforesis Capilar/métodos , Ácidos Fenilpirúvicos/química , Isomerismo , Cinética , Reproducibilidad de los Resultados , beta-Ciclodextrinas/químicaRESUMEN
This study investigated possible correlations between the presence of circulating tumor cells (CTCs) and the pathologic types and staging of non-small cell lung cancer (NSCLC) during the early postoperative period. Sixty-nine patients with NSCLC were enrolled in the study. Clinical staging was performed by postoperative pathological examination and imaging. Multiple mRNA in situ analyses targeting specifically expressed genes were carried out to identify the presence of CTCs. Correlations between age, sex, TNM stage and pathological types with the detection rate of CTCs were also established. The results showed the positivity rate of CTCs in patients >55 years was significantly higher than that of patients <55 years (94.74 vs. 70.97%, P<0.05). There was no significant difference in the positivity rate of CTCs between male and female patients (85.71 vs. 85.29%, P>0.05). The correlations between the detection rate of epithelial type or mixed type CTCs with tumor size, lymph node metastasis and distant metastasis TNM in patients with NSCLC were not significant (P>0.05). However, higher TNM stages correlated with higher detection rates of mesenchymal CTCs (P<0.05). There were also significant differences in the detection rates of CTCs amongst the three different pathologic types (adenocarcinoma, squamous cell and large cell carcinomas) (P<0.05). Based on our results, the detection of mesenchymal CTCs during the early postoperative period can help prognosticate the recurrence and metastasis of NSCLC, which is beneficial to the development of individualized treatment strategies.