Downregulation of CDC42 inhibits the proliferation and stemness of human trophoblast stem cell via EZRIN/YAP inactivation.

Placental dysplasia increases the risk of recurrent spontaneous abortion (RSA). However, the underlying mechanism regulating placental development remains unclear. In this study, we showed that the expression of CDC42 was decreased in the villous tissue of RSA samples compared to healthy controls. Further examination demonstrated that CDC42 deficiency led to the differentiation of human trophoblast stem cells (hTSCs) and inhibited their proliferation. Genetic manipulation of YAP and EZRIN in hTSCs revealed that CDC42 regulates the stemness and proliferation of hTSCs; this is dependent on EZRIN, which translocates YAP into the nucleus. Moreover, the expression pattern of EZRIN, YAP, and Ki67 was also abnormal in the villous tissue of RSA samples, consistent with in vitro experiments. In summary, these findings suggest that the CDC42/EZRIN/YAP pathway plays an important role in placental development.

The Risk of Postpartum Hemorrhage Following Prior Prelabor Cesarean Delivery Stratified by Abnormal Placentation: A Multicenter Historical Cohort Study.

Background: Prior prelabor cesarean delivery (CD) was associated with an increase in the risk of placenta previa (PP) in a second delivery, whether it may impact postpartum hemorrhage (PPH) independent of abnormal placentation. This study aimed to assess the risk of PPH stratified by abnormal placentation following a first CD before the onset of labor (prelabor) or intrapartum CD. Methods: This multicenter, historical cohort study involved singleton, pregnant women at 28 weeks of gestation or greater with a CD history between January 2017 and December 2017 in 11 public tertiary hospitals within 7 provinces of China. PPH was analyzed in the subsequent pregnancy between women with prior prelabor CD and women with intrapartum CD. Furthermore, PPH was analyzed in pregnant women stratified by complications with PP alone [without placenta accreta spectrum (PAS) disorders], complications with PP and PAS, complications with PAS alone (without PP), and normal placentation. We performed multivariate logistic regression to calculate adjusted odds ratios (aOR) and 95% CI controlling for predefined covariates. Results: Out of 10,833 pregnant women, 1,197 (11%) women had a history of intrapartum CD and 9,636 (89%) women had a history of prelabor CD. Prior prelabor CD increased the risk of PP (aOR 1.91, 95% CI 1.40-2.60), PAS (aOR 1.68, 95% CI 1.11-2.24), and PPH (aOR 1.33, 95% CI 1.02-1.75) in a subsequent pregnancy. After stratification by complications with PP alone, PP and PAS, PAS alone, and normal placentation, prior prelabor CD only increased the risk of PPH (aOR 3.34, 95% CI 1.35-8.23) in a subsequent pregnancy complicated with PP and PAS. Conclusion: Compared to intrapartum CD, prior prelabor CD increased the risk of PPH in a subsequent pregnancy only when complicated by PP and PAS.

The risk factors for puncture site bleeding after transfemoral artery puncture intervention:a meta-analysis / 介入放射学杂志

Objective To explore the risk factors for bleeding at the puncture site after femoral artery puncture intervention.Methods A computerized retrieval of observation studies,including cross-sectional studies,case-control studies,and cohort studies,about the risk factors for bleeding at the puncture site after femoral artery puncture intervention from the databases of China National Knowledge Infrastructure(CNKI),Wanfang,VIP,China Biomedical Literature Service(CBM),PubMed,Medline,The Cochrane Library,EMbase and Web of Science was conducted.The retrieval time period was from the establishment of the database to December 31,2022.Newcastle-Ottawa scale(NOS)was used to evaluate the quality of the included studies,and RevMan5.3 software was used to make meta-analysis of the literature data.Results A total of 8 articles with a total sample size of 35 250 patients were included in this analysis.There were 1 410 patients in the postoperative bleeding group and 33 840 patients in the non-bleeding group.The results of the meta-analysis showed that the aged(OR=2.71,95％ CI=2.17-3.38),female(OR=4.26,95％ CI=1.08-16.89),hypertension(OR=2.48,95％CI=1.69-3.63),obesity(OR=2.33,95％ CI=1.59-3.42),use of thrombolytic agents,anticoagulants or platelet antagonists(OR=2.95,95％ CI=2.24-3.89),manual compression(OR=6.78,95％ CI=1.34-34.43)were the risk factors for the bleeding at the puncture site after femoral artery puncture intervention.Conclusion The aged,female,hypertension,obesity,use of thrombolytic agents,anticoagulants or platelet antagonists,and manual compression are the risk factors for the bleeding at the puncture site after femoral artery puncture intervention.(J Intervent Radiol,2024,33:314-320)

Epidemic characteristics of emerging respiratory infectious diseases and coping strategies / 重庆医学

The prevention and control of emerging respiratory infectious diseases is an arduous task. The current outbreak of the new coronavirus pneumonia (COVID-19) is severe and seriously endangers people's health. In order to better guide the clinical work, this article briefly expounded the epidemic characteristics and corresponding coping strategies of the main emerging respiratory infectious diseases in recent years.

Investigation on quality of life and its influencing factors in senior women with urinary incontinence / 中国实用护理杂志

Objective:To investigate the quality of life of senior women with urinary incontinence and to explore its influencing factors.Methods:A convenient sampling method was used to select 152 senior women with urinary incontinence in Fuzhou Hot-Spring Street Community, Dongjie Street Community and Old-age Care Institutions from June 2017 to September 2017. The quality of life of elderly women with urinary incontinence were observed and the influencing factors were analyzed via International Consultation on Incontinence Questionnaire Short Form (ICI-Q-SF) and Incontinence Quality of Life Scale (I-QOL).Results:The quality of life of senior women with urinary incontinence was (98.7±12.2) points. The scores of the three dimensions including restrictive behavior, social activity limitation and psychological impact were 34.5±4.4 and 41.4±5.4 and 20.8 ± 3.5, respectively. There were 4 factors that influencing the quality of life of patients, namely, the severity of urinary incontinence, the type of urinary incontinence, diabetes and the frequency of leaking urine ( P<0.01). Conclusions:Urinary incontinence has a certain negative impact on the quality of life of patients, and its influencing factors are complex. Individualized care measures should be taken based on the influencing factors to improve the quality of life of patients.

Relationship between miR-655 with the clinical features of glioma and its role in diagnosis and prognosis / 国际检验医学杂志

Objective To study the relationship between miR-655 with the clinical features of glioma and its role in diagnosis and prognosis.Methods The tissues and para cancerous tissues of 97 patients with glioma were collected from January 2013 to May 2017 at the Affiliated Hospital of Inner Mongolia Medical Universi-ty.The expression of miR 655 was detected by real-time fluorescence quantitative PCR,and the diagnosis and prognosis of miR-655 in glioma were analyzed.Results The expression of miR-655 in the cancerous tissues was significantly lower than that of the paracancerous tissues of the glioma (P<0.001),which was signifi-cantly related to the size of the tumor (P=0.024) and the WHO grading (P=0.002).COX regression analy-sis showed that the expression of miR-655 (P=0.004)and the degree of tumor resection(P=0.008) were in-dependent risk factors for glioma;ROC analysis showed that the area under the curve of the glioma (AUC) under the miR-655 expression was 0.959 (95% CI:0.936 -0.982);when the expression of miR-655 was 0.755,the sensitivity and specificity of the diagnosis were 89.7% and 88.7% respectively.The Kaplan Meier analysis of the expression of glioma was statistically significant (P=0.004).The lower expression of miR-655 indicated the worse the prognosis of glioma patients,the COX analysis miR-655 was an independent risk factor for the survival of glioma patients.Conclusion miR-655 can be used as a marker of poor prognosis in diagnosis and prognosis.

Survey on replacement care demands of family caregivers of the aged in Zhengzhou city / 中国实用护理杂志

Objective To discuss the demands of replacement care service for family caregivers of the aged in Zhengzhou City,so as to provide the reference for replacement care service of the aged.Methods A total of 145 family caregivers of the aged were collected in Zhengzhou city and were investigated with a self-designed questionnaire.Results In the 145 questionnaires,106 were valid.81 (76.4％) of them expressed needing to be replaced in the process of care,and 68 (64.1％) were willing to accept replacement care service.Order of demands of replacement service content was daily life nursing (77,72.6％),disease (66,62.3％) and psychological care (60,56.6％),etc.Influencing factors of selecting replacement care service for family caregivers included quality of service (63,59.4％),service price (38,35.8％) and professional skill (37,34.9％).The degree of demand of different ages,different careers,as well as different duration of daily care of family caregivers was statistically significant for replacement care service.Conclusions The degree of demand of family caregivers of the aged for replacement care service was higher.The government needs to establish replacement care service mechanism which is suitable for China's national conditions through policy guidance and support.

Clinical analysis of 23 cases of infantile diarrhea with intussusception / 中国医师进修杂志

Objective To investigate the clinical features of infantile diarrhea with intussusception,improve the level of diagnosis and treatment,reduce the misdiagnosis and delayed treatment.Methods The clinical data of 23 cases with infantile intussusception were retrospectively analyzed.Results Twenty-three cases of children with diarrhea symptoms at the beginning of the disease had different degree,12 cases characterized by vomiting,diarrhea,fever and other symptoms of acute gastroenteritis,5 cases stool were mucous pus blood,6 cases for blood in the stool.Five cases were confirmed within 24 h of the diagnosis (3 cases with air enema reduction of intussusception,2 cases with manual reduction),11 cases were confirmed at 24 h to 3 d of the diagnosis and operated,7 cases were confirmed more than 3 d of the diagnosis and operated.Except 1 cases was died from shock,22 cases were cured.Conclusion Infantile intussusception atypical symptoms,illness development is rapid,in critical condition,easy misdiagnosis,missed diagnosis,should actively improve the standard of diagnosis.,early diagnosis,so as not to delay treatment.

Discussion on How to maintain the path of hospital's scientific research sustainable development / 中华医学科研管理杂志

Medical scientific research is the main driving force of development of medical science.In order to ensure the Hospital take the road of sustainable development in a market economy environment,We must rely on technologIcal progress and innovation to improve the comprehensive competitiveness of the hospital.Through analysis the present of our hospital scientific research,Proposed the bottleneck problem how to maintain the momentum of scientific research development.And on this basis investigate the hospital scientific research trends:Achieve major breakthrough in scientific research; To Create scientific For the sake of research team with strong competitiveness,To Strengthen personnel training gradient,reserve new force for development of hospital's scientific research; Apply for the high level scientific research projects,Strengthen the regulatory regime of the scientific research projects; Around a stable long-term scientific research direction to do depth research; Ensure The hospital's scientific research work on a sustainable development path.

Change of the guanine nucleotide-binding protein in lamina Ⅰ-Ⅲ of spinal cord posterior horn and lateral spinal nucleus of rats after transection of dorsal roots / 中国组织工程研究

BACKGROUND: In China, this laboratory is the first one to report such researches, confirming that strong αo-immunoreactive (IR) appears in the substantia gelatinosa (SG) of spinal cord and lateral spinal nucleus which is similar to the distribution of certain neuropeptides that participate in sensory regulation, which suggests that guanine nucleotide binding protein (G protein) may be related to primary afferent informational transfer. OBJECTIVE: To observe the change of αo-IR in gelatinous substance by the method of transection of unilateral spinal dorsal roots.DESIGN: A randomized controlled experiment on animals.SETTING: Staff Room of Neurobiology, Tongji Medical College, Huazhong University of Science and Technology.MATERIALS: The experiment was conducted at the Staff Room of Neurobiology, Tongji Medical College, Huazhong University of Science and Technology from December 1995 to December 1996. Fifteen healthy adult SD rats were selected and divided into 3 groups: ①normal group with five rats (not dealt with any disposal), ②transected dorsal root group with 10 rats (right side) and ③control group (non-transected left sidedness as control).METHODS: Right lumbar 1-3 spinal neural dorsal roots were cut off under the anesthesia of 100 g/L chloral hydrate (300 mg/kg)through intraperitoneal injection in rats, living for 48-60 hours after operation. The subunit αo of guanine nucleotide-binding protein (rabbit polyclonal antiserum) was demonstrated in the αo-IR of rat spinal cord by immunohisto chemical methods. G protein was oriented, and its change was observed after transection ofneural dorsal roots MAIN OUTCOME MEASURES: ①The αo-IR of Ⅰ to Ⅲ of the dorsal horn and lateral spinal nucleus of the normal rats and control rats. ②The αo-IR of Ⅰ to Ⅲ of the dorsal horn and lateral spinal nucleus of rats in the transected dorsal root group. RESULTS: Data of a total of 15 rats were involved in the result analysis. ①In the normal group and control group, intense αo-IR was presented in rexed lamina ( Ⅰ to Ⅲ ) of the dorsal horn of rats, and the highest αo-IR in second lamina (SG). Lateral spinal nucleus of rat revealed higher density of αo-IR containing fiber networks. Following unilateral transection of dorsal roots in SG, αo-IR was markedly decreased. ②Quantitative analysis of absorbance (A) of αo-IR, it was (0.847±0.081) in the inside of the control group, (0.633±0.073)(t=5.71 ,P ＜ 0.001 ) in the inside of transected dorsal root group. It was (0.823±0.089) in the middle area of the control group,(0.660 4±0.074)(t=6.90,P ＜ 0.001 ) in the middle area of the transected dorsal root group. It was (0.915±0.090) in the lumbar region of the control group, and (0.656±0.077)(t=10.31 ,P ＜ 0.001 ) in the lumbar region of the transected dorsal root group. Average value of the control group was (0.852±0.084), and average value of the transected dorsal root group was (0.639±0.078)(t=10.23 ,P ＜ 0.001 ).CONCLUSION: Part of G protein of end-brush neurons related with the primary afferent noxious stimulation in SG derives from primary sensory neurons, which maybe join the adjustment of primary sensory transfer.

The study of humoral immunity function and the short- and long-term effective of immunoglobulin treatment in intractable childhood epilepsy / 中国免疫学杂志

Objective:To explore the short and long term effective and the changes of the immunoglobulin and complements after immunoglobulins therapy in intractable childhood epilepsy.Methods:To observe the clinical efficacy of 6 month～1 year in 36 cases with intractable epilepsy was treated added immunoglobulins and to detect the immunoglobulins and complements 3、4、CH50 which were measured by using ELISA and nephelometry method in them and 22 healthy children were control group and to comparing the changers among them in match before and after treatment.Results:The result showed that the efficacy was 17 cases to be ceased entirely,in 5 cases the attacks markedly improved, the other 6 cases improvement too,and remained 8 cases unchanged, the total effective rate of short term was 77 8%,28 cases following over one years, of them 13(46 4%)attacks repeated, the long term effective rate was 53 6%.The present time of clinical efficacy was in a week,EEG improvement at same time.The results of humoral immunity Ig and C3,C4,CH50 were lower in epilepsy group than in control group (P

The research on inflammatory factors of serum in the patient with Parkinson's disease / 中国免疫学杂志

Objective:To detect the level of immune related cytokines in the serum of the patient with Parkinson’s Disease (PD) and to explore the influencing factors of the cytokines.Methods:51 patients with PD (PD group) and 35 healthy control (control group) were studied.The two groups were detected the serum concentration of IL-1?,IL-2,IL-6 and TNF-? by the way of radio immunity.Results:The serum level of IL-6 and TNF-? in the PD group is significantly higher than that of the control group (P