Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Resultados 1 - 2 de 2
Filtrar
Más filtros

Banco de datos
Tipo del documento
Publication year range
1.
Biochem Biophys Res Commun ; 641: 102-109, 2023 01 22.
Artículo en Inglés | MEDLINE | ID: mdl-36525924

RESUMEN

The premature death and degeneration of striatal neurons are typical hallmarks of HtrA2-inactivated motor neuron degeneration 2 (mnd2) mice. Although HtrA2 has been extensively studied in relation to the regulation of apoptosis using mnd2 mice, little is known about the other physiological functions of HtrA2. In this study, we found that the skin color of wild-type (WT) and mnd2 mice was black and pink on postnatal day 32. Using histological and molecular assays (i.e., assessing the activation of MAPK and expression patterns of PCNA), we demonstrated that this differential skin color change is consistent with the delay in the telogen - to - anagen phase of the hair cycle in mnd2 mice. We also examined adipocytes in the subcutaneous skin layer, finding that HtrA2 inactivation leads to the growth retardation of adipocytes, thereby delaying the hair cycle of mnd2 mice. Collectively, these findings show for the first time that HtrA2 plays an essential role in regulating the adipogenesis-associated hair cycle.


Asunto(s)
Proteínas Mitocondriales , Serina Endopeptidasas , Animales , Ratones , Apoptosis , Cabello/metabolismo , Serina Peptidasa A2 que Requiere Temperaturas Altas/genética , Proteínas Mitocondriales/metabolismo , Serina Endopeptidasas/genética , Serina Endopeptidasas/metabolismo
2.
Exp Mol Med ; 56(4): 890-903, 2024 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-38556552

RESUMEN

Acute phase proteins involved in chronic inflammatory diseases have not been systematically analyzed. Here, global proteome profiling of serum and urine revealed that orosomucoid-2 (ORM2), an acute phase reactant, was differentially expressed in rheumatoid arthritis (RA) patients and showed the highest fold change. Therefore, we questioned the extent to which ORM2, which is produced mainly in the liver, actively participates in rheumatoid inflammation. Surprisingly, ORM2 expression was upregulated in the synovial fluids and synovial membranes of RA patients. The major cell types producing ORM2 were synovial macrophages and fibroblast-like synoviocytes (FLSs) from RA patients. Recombinant ORM2 robustly increased IL-6, TNF-α, CXCL8 (IL-8), and CCL2 production by RA macrophages and FLSs via the NF-κB and p38 MAPK pathways. Interestingly, glycophorin C, a membrane protein for determining erythrocyte shape, was the receptor for ORM2. Intra-articular injection of ORM2 increased the severity of arthritis in mice and accelerated the infiltration of macrophages into the affected joints. Moreover, circulating ORM2 levels correlated with RA activity and radiographic progression. In conclusion, the acute phase protein ORM2 can directly increase the production of proinflammatory mediators and promote chronic arthritis in mice, suggesting that ORM2 could be a new therapeutic target for RA.


Asunto(s)
Artritis Reumatoide , Macrófagos , Orosomucoide , Artritis Reumatoide/metabolismo , Artritis Reumatoide/patología , Humanos , Animales , Orosomucoide/metabolismo , Ratones , Macrófagos/metabolismo , Masculino , Femenino , Membrana Sinovial/metabolismo , Membrana Sinovial/patología , Proteínas de Fase Aguda/metabolismo , Sinoviocitos/metabolismo , Sinoviocitos/patología , Citocinas/metabolismo , Persona de Mediana Edad , Líquido Sinovial/metabolismo , Inflamación/metabolismo , Inflamación/patología , Biomarcadores , Mediadores de Inflamación/metabolismo , Modelos Animales de Enfermedad
SELECCIÓN DE REFERENCIAS
Detalles de la búsqueda