in vivo Evaluation of Immunomodulatory Activity of Lipopolysaccharide Zinc Oxide Nanoparticles (LPS-ZnNPS).

Klebsiella pneumoniae causes a wide variety of infectious diseases. Lipopolysaccharide (LPS) is a large heat-stable polymer that is gram-negative bacteria's major outer membrane component, accounting for roughly 75% of the surface area and 5-10% of the total dry weight. Therefore the current in vivo study was carried out to investigate the immunomodulatory effect of purified lipopolysaccharide produced from local clinical Klebsiella pneumoniae isolates compared with ZnO-NPs and LPS-ZnO NPs. To do the experimental evaluations 35, Balb/c mouse was injected intramuscularly (i.m.) with different concentrations of the purified LPS, ZnoNPs and LPS-ZnoNPs for 12 days and immunized with 10% SRBCs (i.p) on day 4 and 8 of the schedule, while K. pneumoniae suspension and normal saline for positive and negative control groups. Focus on estimating body weight before and after treatment, Arthus and delayed-type hypersensitivity, and detecting serum level of cytokines (TLR-2, IL1Beta, IL4, and IL10) using sandwich ELISA. The data showed the highest value before and after treatment with LPS-ZnO NPs recorded in 2µg/mouse was 27. 92±1.48 and 31.50±0.4, respectively. In Arthurs reaction and Delayed type hypersensitivity, the highest results showed in the positive control group injected with K. pneumoniae 4.08±0.17 and 4.86±80.02, respectively. The results of TLR-2 showed the highest value in the positive control group, 242.17±3.98 pg/ml, followed by Group LPS at 135.51.58 pg/ml. The results of Interleukin-1Beta showed the highest value in the positive control group, 254.88±3.51 pg/ml, followed by Group LPS 174.3± 1.46 pg/ml. The concentration of IL-4 in serum of treated albino mice showed the highest value in the positive control group, 136.2±1.12 pg/ml, followed by Group LPS 86.12±1.49 pg/ml. While the highest value of IL-10 was recorded in the positive control group, 98.58± 4.09 pg/ml, followed by Group LPS- ZnoNP in concentration 4µg/ mouse was 86.018±0.69 pg/ml. The results of the statistical analysis showed a significant difference (P≤0.05) between LPS, ZnoNPs, and LPs-ZnoNPs treated groups and control groups (positive & negative). In the present study, we can conclude that LPS-ZnO NPs had a positive immunomodulatory effect on immune response in immunized mice. As shown in the results of the level of IL-1 beta, IL-4, IL-10, and TLRs-2, Abs titer, and Arthus and DTH reactions.

Assessment and upgrading of Alexandria University nursing students' knowledge and attitudes about genetic counseling.

The aim of this study was to study the effect of a teaching unit on upgrading university-nursing students' knowledge and attitudes about genetic counseling. The study used pre- test, posttest with no control group design. All nursing students (100) who accepted to participate in the study and enrolled in the Maternity and Gynecological-nursing course, during the second semester of the third academic year 1999-2000, at the Faculty of Nursing, Alexandria University, were involved in the study. A self-administered questionnaire sheet was used to assess nursing students' knowledge about genetic counseling, while a 3-point Likert-like scale was used to assess their attitudes towards it. The content of six-hours' teaching unit -about genetic counseling was taught to students by the researchers based on their needs. The study revealed a general lack of knowledge among nursing students regarding basic information about genetic counseling, where poor total score of knowledge was obtained by all of them in the pre-test. In addition, the pre-test revealed that negative attitude was found in about half of the nursing students. The teaching unit had an obvious effect on the nursing students' knowledge and attitudes regarding genetic counseling, as the total score of their knowledge and attitudes had improved after implementation of the teaching unit. Moreover, there was a significant relationship between nursing students' knowledge and attitudes towards genetic counseling before and after implementation of the teaching unit. Therefore, a negative attitude was found with poor total score of knowledge in the pre-test and positive attitude was found with good total score of knowledge in the post-test. So, there is a need to improve knowledge and change attitude of nursing students about genetic counseling. As well as genetic counseling should be included in the university-nursing curriculum and should be reviewed periodically to accommodate the relevant fast change in the science and technology of genetics. Moreover, nursing students must be informed about the different community resources, which offer the genetic counseling services.