RESUMEN
A new, simplified methodology of preparation of lyophilized chrome-coated turkey blood cells (formalin-treated ) for rapid identification of tetanus antibodies in indirect hemagglutination test is described. Blood cells diagnostic preparations obtained in this way were easier and three times faster to prepare than tanned cell preparations . They maintained unchanged capability for specific agglutination during at least one year of storage when kept at 4 degrees C. proposed methodology enable to start a production of laboratory kits necessary for controlled prophylaxis of tetanus.
Asunto(s)
Anticuerpos Antibacterianos/análisis , Clostridium tetani/inmunología , Tétanos/inmunología , Animales , Cromo/farmacología , Eritrocitos/efectos de los fármacos , Eritrocitos/inmunología , Pruebas de Hemaglutinación/métodos , Humanos , Técnicas In Vitro , Taninos/farmacología , Tétanos/diagnóstico , Factores de Tiempo , Pavos/sangreRESUMEN
Seventy-two normal human sera from healthy blood donors were tested by an enzyme-linked immunosorbent assay in order to determine the amounts and avidities of immunoglobulins M and G antibodies to lipopolysaccharides of seven Fisher's immunotypes of Pseudomonas aeruginosa and to exotoxin A. The patterns of specificity for seven immunotypes in all individual sera were determined. These data show a predominance of antibodies directed to Fisher's immunotypes 7 and 4 in the human population tested and may reflect frequency of occurrence of immunotypes outside the hospital environment.
Asunto(s)
ADP Ribosa Transferasas , Anticuerpos Antibacterianos/análisis , Antígenos Bacterianos/inmunología , Toxinas Bacterianas , Pseudomonas aeruginosa/inmunología , Factores de Virulencia , Anticuerpos Antibacterianos/inmunología , Especificidad de Anticuerpos , Donantes de Sangre , Ensayo de Inmunoadsorción Enzimática , Exotoxinas/inmunología , Humanos , Sueros Inmunes/inmunología , Inmunoglobulina G/análisis , Inmunoglobulina G/inmunología , Inmunoglobulina M/análisis , Inmunoglobulina M/inmunología , Lipopolisacáridos/inmunología , Exotoxina A de Pseudomonas aeruginosaRESUMEN
ELISA was used to measure the amount and avidity of IgG antibodies to exotoxin A (ExA) and 7 Fisher's immunotypes of lipopolysaccharide (LPS) in the sera of 13 patients with mild or moderate Pseudomonas aeruginosa infections. Changes in the specificity of tested sera during the course of infection were demonstrated. A statistically significant increase was seen in the amount and avidity of the antibodies to ExA in a majority of the sera, and an increase was seen in amount of antibodies to LPS immunotype 4 in the sera of patients with moderate infections.