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1.
Genet Epidemiol ; 44(4): 339-351, 2020 06.
Artículo en Inglés | MEDLINE | ID: mdl-32100375

RESUMEN

Testing millions of single nucleotide polymorphisms (SNPs) in genetic association studies has become a standard routine for disease gene discovery. In light of recent re-evaluation of statistical practice, it has been suggested that p-values are unfit as summaries of statistical evidence. Despite this criticism, p-values contain information that can be utilized to address the concerns about their flaws. We present a new method for utilizing evidence summarized by p-values for estimating odds ratio (OR) based on its approximate posterior distribution. In our method, only p-values, sample size, and standard deviation for ln(OR) are needed as summaries of data, accompanied by a suitable prior distribution for ln(OR) that can assume any shape. The parameter of interest, ln(OR), is the only parameter with a specified prior distribution, hence our model is a mix of classical and Bayesian approaches. We show that our method retains the main advantages of the Bayesian approach: it yields direct probability statements about hypotheses for OR and is resistant to biases caused by selection of top-scoring SNPs. Our method enjoys greater flexibility than similarly inspired methods in the assumed distribution for the summary statistic and in the form of the prior for the parameter of interest. We illustrate our method by presenting interval estimates of effect size for reported genetic associations with lung cancer. Although we focus on OR, the method is not limited to this particular measure of effect size and can be used broadly for assessing reliability of findings in studies testing multiple predictors.


Asunto(s)
Susceptibilidad a Enfermedades , Modelos Genéticos , Teorema de Bayes , Sitios Genéticos , Humanos , Polimorfismo de Nucleótido Simple
2.
PLoS Comput Biol ; 16(4): e1007819, 2020 04.
Artículo en Inglés | MEDLINE | ID: mdl-32287273

RESUMEN

Historically, the majority of statistical association methods have been designed assuming availability of SNP-level information. However, modern genetic and sequencing data present new challenges to access and sharing of genotype-phenotype datasets, including cost of management, difficulties in consolidation of records across research groups, etc. These issues make methods based on SNP-level summary statistics particularly appealing. The most common form of combining statistics is a sum of SNP-level squared scores, possibly weighted, as in burden tests for rare variants. The overall significance of the resulting statistic is evaluated using its distribution under the null hypothesis. Here, we demonstrate that this basic approach can be substantially improved by decorrelating scores prior to their addition, resulting in remarkable power gains in situations that are most commonly encountered in practice; namely, under heterogeneity of effect sizes and diversity between pairwise LD. In these situations, the power of the traditional test, based on the added squared scores, quickly reaches a ceiling, as the number of variants increases. Thus, the traditional approach does not benefit from information potentially contained in any additional SNPs, while our decorrelation by orthogonal transformation (DOT) method yields steady gain in power. We present theoretical and computational analyses of both approaches, and reveal causes behind sometimes dramatic difference in their respective powers. We showcase DOT by analyzing breast cancer and cleft lip data, in which our method strengthened levels of previously reported associations and implied the possibility of multiple new alleles that jointly confer disease risk.


Asunto(s)
Biología Computacional/métodos , Estudio de Asociación del Genoma Completo/métodos , Desequilibrio de Ligamiento/genética , Polimorfismo de Nucleótido Simple/genética , Neoplasias de la Mama/genética , Labio Leporino/genética , Femenino , Marcadores Genéticos/genética , Predisposición Genética a la Enfermedad/genética , Humanos , Modelos Estadísticos
3.
Genet Epidemiol ; 41(8): 726-743, 2017 12.
Artículo en Inglés | MEDLINE | ID: mdl-28913944

RESUMEN

The increasing accessibility of data to researchers makes it possible to conduct massive amounts of statistical testing. Rather than follow specific scientific hypotheses with statistical analysis, researchers can now test many possible relationships and let statistics generate hypotheses for them. The field of genetic epidemiology is an illustrative case, where testing of candidate genetic variants for association with an outcome has been replaced by agnostic screening of the entire genome. Poor replication rates of candidate gene studies have improved dramatically with the increase in genomic coverage, due to factors such as adoption of better statistical practices and availability of larger sample sizes. Here, we suggest that another important factor behind the improved replicability of genome-wide scans is an increase in the amount of statistical testing itself. We show that an increase in the number of tested hypotheses increases the proportion of true associations among the variants with the smallest P-values. We develop statistical theory to quantify how the expected proportion of genuine signals (EPGS) among top hits depends on the number of tests. This enrichment of top hits by real findings holds regardless of whether genome-wide statistical significance has been reached in a study. Moreover, if we consider only those "failed" studies that produce no statistically significant results, the same enrichment phenomenon takes place: the proportion of true associations among top hits grows with the number of tests. The enrichment occurs even if the true signals are encountered at the logarithmically decreasing rate with the additional testing.


Asunto(s)
Modelos Genéticos , Teorema de Bayes , Estudio de Asociación del Genoma Completo , Humanos , Modelos Estadísticos
4.
Genet Epidemiol ; 40(3): 210-221, 2016 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-27027515

RESUMEN

Recent technological advances equipped researchers with capabilities that go beyond traditional genotyping of loci known to be polymorphic in a general population. Genetic sequences of study participants can now be assessed directly. This capability removed technology-driven bias toward scoring predominantly common polymorphisms and let researchers reveal a wealth of rare and sample-specific variants. Although the relative contributions of rare and common polymorphisms to trait variation are being debated, researchers are faced with the need for new statistical tools for simultaneous evaluation of all variants within a region. Several research groups demonstrated flexibility and good statistical power of the functional linear model approach. In this work we extend previous developments to allow inclusion of multiple traits and adjustment for additional covariates. Our functional approach is unique in that it provides a nuanced depiction of effects and interactions for the variables in the model by representing them as curves varying over a genetic region. We demonstrate flexibility and competitive power of our approach by contrasting its performance with commonly used statistical tools and illustrate its potential for discovery and characterization of genetic architecture of complex traits using sequencing data from the Dallas Heart Study.


Asunto(s)
Estudios de Asociación Genética , Modelos Lineales , Fenotipo , Negro o Afroamericano/genética , Proteína 4 Similar a la Angiopoyetina , Angiopoyetinas/genética , Femenino , Genotipo , Corazón , Hispánicos o Latinos/genética , Humanos , Masculino , Modelos Genéticos , Polimorfismo Genético/genética , Encuestas y Cuestionarios , Texas , Triglicéridos/sangre , Población Blanca/genética
5.
Breast Cancer Res Treat ; 161(2): 333-344, 2017 01.
Artículo en Inglés | MEDLINE | ID: mdl-27848153

RESUMEN

PURPOSE: Genome-wide association studies (GWAS) have identified dozens of single-nucleotide polymorphisms (SNPs) associated with breast cancer. Few studies focused on young-onset breast cancer, which exhibits etiologic and tumor-type differences from older-onset disease. Possible confounding by prenatal effects of the maternal genome has also not been considered. METHODS: Using a family-based design for breast cancer before age 50, we assessed the relationship between breast cancer and 77 GWAS-identified breast cancer risk SNPs. We estimated relative risks (RR) for inherited and maternally mediated genetic effects. We also used published RR estimates to calculate genetic risk scores and model joint effects. RESULTS: Seventeen of the candidate SNPs were nominally associated with young-onset breast cancer in our 1296 non-Hispanic white affected families (uncorrected p value <0.05). Top-ranked SNPs included rs3803662-A (TOX3, RR = 1.39; p = 7.0 × 10-6), rs12662670-G (ESR1, RR = 1.56; p = 5.7 × 10-4), rs2981579-A (FGFR2, RR = 1.24; p = 0.002), and rs999737-G (RAD51B, RR = 1.37; p = 0.003). No maternally mediated effects were found. A risk score based on all 77 SNPs indicated that their overall relationship to young-onset breast cancer risk was more than additive (additive-fit p = 2.2 × 10-7) and consistent with a multiplicative joint effect (multiplicative-fit p = 0.27). With the multiplicative formulation, the case sister's genetic risk score exceeded that of her unaffected sister in 59% of families. CONCLUSIONS: The results of this family-based study indicate that no effects of previously identified risk SNPs were explained by prenatal effects of maternal variants. Many of the known breast cancer risk variants were associated with young-onset breast cancer, with evidence that TOX3, ESR1, FGFR2, and RAD51B are important for young-onset disease.


Asunto(s)
Neoplasias de la Mama/epidemiología , Neoplasias de la Mama/genética , Predisposición Genética a la Enfermedad , Estudio de Asociación del Genoma Completo , Adulto , Edad de Inicio , Alelos , Etnicidad , Femenino , Genotipo , Humanos , Persona de Mediana Edad , Oportunidad Relativa , Polimorfismo de Nucleótido Simple , Medición de Riesgo , Factores de Riesgo , Adulto Joven
6.
Proc Natl Acad Sci U S A ; 111(16): E1581-90, 2014 Apr 22.
Artículo en Inglés | MEDLINE | ID: mdl-24711389

RESUMEN

Identification of genes associated with specific biological phenotypes is a fundamental step toward understanding the molecular basis underlying development and pathogenesis. Although RNAi-based high-throughput screens are routinely used for this task, false discovery and sensitivity remain a challenge. Here we describe a computational framework for systematic integration of published gene expression data to identify genes defining a phenotype of interest. We applied our approach to rank-order all genes based on their likelihood of determining ES cell (ESC) identity. RNAi-mediated loss-of-function experiments on top-ranked genes unearthed many novel determinants of ESC identity, thus validating the derived gene ranks to serve as a rich and valuable resource for those working to uncover novel ESC regulators. Underscoring the value of our gene ranks, functional studies of our top-hit Nucleolin (Ncl), abundant in stem and cancer cells, revealed Ncl's essential role in the maintenance of ESC homeostasis by shielding against differentiation-inducing redox imbalance-induced oxidative stress. Notably, we report a conceptually novel mechanism involving a Nucleolin-dependent Nanog-p53 bistable switch regulating the homeostatic balance between self-renewal and differentiation in ESCs. Our findings connect the dots on a previously unknown regulatory circuitry involving genes associated with traits in both ESCs and cancer and might have profound implications for understanding cell fate decisions in cancer stem cells. The proposed computational framework, by helping to prioritize and preselect candidate genes for tests using complex and expensive genetic screens, provides a powerful yet inexpensive means for identification of key cell identity genes.


Asunto(s)
Células Madre Embrionarias/citología , Células Madre Embrionarias/metabolismo , Homeostasis/genética , Animales , Diferenciación Celular/genética , Proliferación Celular , Regulación de la Expresión Génica , Proteínas de Homeodominio/metabolismo , Ratones , Proteína Homeótica Nanog , Estrés Oxidativo/genética , Fosfoproteínas/genética , Fosfoproteínas/metabolismo , Células Madre Pluripotentes/citología , Células Madre Pluripotentes/metabolismo , Interferencia de ARN , Proteínas de Unión al ARN/genética , Proteínas de Unión al ARN/metabolismo , Especies Reactivas de Oxígeno/metabolismo , Reproducibilidad de los Resultados , Transcripción Genética , Proteína p53 Supresora de Tumor/metabolismo , Nucleolina
7.
Arthritis Rheum ; 64(2): 584-93, 2012 Feb.
Artículo en Inglés | MEDLINE | ID: mdl-21905019

RESUMEN

OBJECTIVE: Fibromyalgia (FM) represents a complex disorder that is characterized by widespread pain and tenderness and is frequently accompanied by additional somatic and cognitive/affective symptoms. Genetic risk factors are known to contribute to the etiology of the syndrome. The aim of this study was to examine >350 genes for association with FM, using a large-scale candidate gene approach. METHODS: The study group comprised 496 patients with FM (cases) and 348 individuals with no chronic pain (controls). Genotyping was performed using a dedicated gene array chip, the Pain Research Panel, which assays variants characterizing >350 genes known to be involved in the biologic pathways relevant to nociception, inflammation, and mood. Association testing was performed using logistic regression. RESULTS: Significant differences in allele frequencies between cases and controls were observed for 3 genes: GABRB3 (rs4906902; P = 3.65 × 10(-6)), TAAR1 (rs8192619; P = 1.11 × 10(-5)), and GBP1 (rs7911; P = 1.06 × 10(-4)). These 3 genes and 7 other genes with suggestive evidence for association were examined in a second, independent cohort of patients with FM and control subjects who were genotyped using the Perlegen 600K platform. Evidence of association in the replication cohort was observed for TAAR1, RGS4, CNR1, and GRIA4. CONCLUSION: Variation in these 4 replicated genes may serve as a basis for development of new diagnostic approaches, and the products of these genes may contribute to the pathophysiology of FM and represent potential targets for therapeutic action.


Asunto(s)
Fibromialgia/genética , Predisposición Genética a la Enfermedad , Polimorfismo de Nucleótido Simple , Adulto , Anciano , Alelos , Estudios de Casos y Controles , Femenino , Proteínas de Unión al GTP/genética , Frecuencia de los Genes , Estudios de Asociación Genética , Genotipo , Humanos , Persona de Mediana Edad , Receptores Acoplados a Proteínas G/genética , Receptores de GABA-B/genética
8.
Nucleic Acids Res ; 39(Database issue): D730-5, 2011 Jan.
Artículo en Inglés | MEDLINE | ID: mdl-21113022

RESUMEN

DOMINE is a comprehensive collection of known and predicted domain-domain interactions (DDIs) compiled from 15 different sources. The updated DOMINE includes 2285 new domain-domain interactions (DDIs) inferred from experimentally characterized high-resolution three-dimensional structures, and about 3500 novel predictions by five computational approaches published over the last 3 years. These additions bring the total number of unique DDIs in the updated version to 26,219 among 5140 unique Pfam domains, a 23% increase compared to 20,513 unique DDIs among 4346 unique domains in the previous version. The updated version now contains 6634 known DDIs, and features a new classification scheme to assign confidence levels to predicted DDIs. DOMINE will serve as a valuable resource to those studying protein and domain interactions. Most importantly, DOMINE will not only serve as an excellent reference to bench scientists testing for new interactions but also to bioinformaticans seeking to predict novel protein-protein interactions based on the DDIs. The contents of the DOMINE are available at http://domine.utdallas.edu.


Asunto(s)
Bases de Datos de Proteínas , Dominios y Motivos de Interacción de Proteínas , Mapeo de Interacción de Proteínas
9.
Proc Natl Acad Sci U S A ; 107(11): 5148-53, 2010 Mar 16.
Artículo en Inglés | MEDLINE | ID: mdl-20212137

RESUMEN

The gene SCN9A is responsible for three human pain disorders. Nonsense mutations cause a complete absence of pain, whereas activating mutations cause severe episodic pain in paroxysmal extreme pain disorder and primary erythermalgia. This led us to investigate whether single nucleotide polymorphisms (SNPs) in SCN9A were associated with differing pain perception in the general population. We first genotyped 27 SCN9A SNPs in 578 individuals with a radiographic diagnosis of osteoarthritis and a pain score assessment. A significant association was found between pain score and SNP rs6746030; the rarer A allele was associated with increased pain scores compared to the commoner G allele (P = 0.016). This SNP was then further genotyped in 195 pain-assessed people with sciatica, 100 amputees with phantom pain, 179 individuals after lumbar discectomy, and 205 individuals with pancreatitis. The combined P value for increased A allele pain was 0.0001 in the five cohorts tested (1277 people in total). The two alleles of the SNP rs6746030 alter the coding sequence of the sodium channel Nav1.7. Each was separately transfected into HEK293 cells and electrophysiologically assessed by patch-clamping. The two alleles showed a difference in the voltage-dependent slow inactivation (P = 0.042) where the A allele would be predicted to increase Nav1.7 activity. Finally, we genotyped 186 healthy females characterized by their responses to a diverse set of noxious stimuli. The A allele of rs6746030 was associated with an altered pain threshold and the effect mediated through C-fiber activation. We conclude that individuals experience differing amounts of pain, per nociceptive stimulus, on the basis of their SCN9A rs6746030 genotype.


Asunto(s)
Dolor/genética , Percepción , Polimorfismo de Nucleótido Simple/genética , Canales de Sodio/genética , Adulto , Alelos , Fenómenos Biofísicos/genética , Estudios de Cohortes , Femenino , Predisposición Genética a la Enfermedad , Humanos , Proteínas Mutantes/genética , Canal de Sodio Activado por Voltaje NAV1.7 , Dolor/fisiopatología , Umbral del Dolor , Análisis de Regresión
10.
Genet Epidemiol ; 34(7): 725-38, 2010 Nov.
Artículo en Inglés | MEDLINE | ID: mdl-20976797

RESUMEN

An appealing genome-wide association study design compares one large control group against several disease samples. A pioneering study by the Wellcome Trust Case Control Consortium that employed such a design has identified multiple susceptibility regions, many of which have been independently replicated. While reusing a control sample provides effective utilization of data, it also creates correlation between association statistics across diseases. An observation of a large association statistic for one of the diseases may greatly increase chances of observing a spuriously large association for a different disease. Accounting for the correlation is also particularly important when screening for SNPs that might be involved in a set of diseases with overlapping etiology. We describe methods that correct association statistics for dependency due to shared controls, and we describe ways to obtain a measure of overall evidence and to combine association signals across multiple diseases. The methods we describe require no access to individual subject data, instead, they efficiently utilize information contained in P-values for association reported for individual diseases. P-value based combined tests for association are flexible and essentially as powerful as the approach based on aggregating the individual subject data.


Asunto(s)
Estudio de Asociación del Genoma Completo/métodos , Estudios de Casos y Controles , Distribución de Chi-Cuadrado , Simulación por Computador , Bases de Datos Genéticas , Estudio de Asociación del Genoma Completo/estadística & datos numéricos , Humanos , Modelos Genéticos , Epidemiología Molecular , Método de Montecarlo , Polimorfismo de Nucleótido Simple
11.
Hum Mol Genet ; 18(6): 1037-51, 2009 Mar 15.
Artículo en Inglés | MEDLINE | ID: mdl-19103668

RESUMEN

The mu-opioid receptor (OPRM1) is the principal receptor target for both endogenous and exogenous opioid analgesics. There are substantial individual differences in human responses to painful stimuli and to opiate drugs that are attributed to genetic variations in OPRM1. In searching for new functional variants, we employed comparative genome analysis and obtained evidence for the existence of an expanded human OPRM1 gene locus with new promoters, alternative exons and regulatory elements. Examination of polymorphisms within the human OPRM1 gene locus identified strong association between single nucleotide polymorphism (SNP) rs563649 and individual variations in pain perception. SNP rs563649 is located within a structurally conserved internal ribosome entry site (IRES) in the 5'-UTR of a novel exon 13-containing OPRM1 isoforms (MOR-1K) and affects both mRNA levels and translation efficiency of these variants. Furthermore, rs563649 exhibits very strong linkage disequilibrium throughout the entire OPRM1 gene locus and thus affects the functional contribution of the corresponding haplotype that includes other functional OPRM1 SNPs. Our results provide evidence for an essential role for MOR-1K isoforms in nociceptive signaling and suggest that genetic variations in alternative OPRM1 isoforms may contribute to individual differences in opiate responses.


Asunto(s)
Polimorfismo de Nucleótido Simple/genética , Receptores Opioides mu/genética , Adolescente , Adulto , Alelos , Animales , Secuencia de Bases , Estudios de Cohortes , Exones/genética , Femenino , Predisposición Genética a la Enfermedad , Haplotipos , Humanos , Intrones/genética , Ratones , Datos de Secuencia Molecular , Conformación de Ácido Nucleico , Dolor/genética , Isoformas de Proteínas/genética , Empalme del ARN/genética
12.
Sleep ; 44(3)2021 03 12.
Artículo en Inglés | MEDLINE | ID: mdl-33034629

RESUMEN

Poor sleep quality can have harmful health consequences. Although many aspects of sleep are heritable, the understandings of genetic factors involved in its physiology remain limited. Here, we performed a genome-wide association study (GWAS) using the Pittsburgh Sleep Quality Index (PSQI) in a multi-ethnic discovery cohort (n = 2868) and found two novel genome-wide loci on chromosomes 2 and 7 associated with global sleep quality. A meta-analysis in 12 independent cohorts (100 000 individuals) replicated the association on chromosome 7 between NPY and MPP6. While NPY is an important sleep gene, we tested for an independent functional role of MPP6. Expression data showed an association of this locus with both NPY and MPP6 mRNA levels in brain tissues. Moreover, knockdown of an orthologue of MPP6 in Drosophila melanogaster sleep center neurons resulted in decreased sleep duration. With convergent evidence, we describe a new locus impacting human variability in sleep quality through known NPY and novel MPP6 sleep genes.


Asunto(s)
Drosophila melanogaster , Estudio de Asociación del Genoma Completo , Animales , Etnicidad , Predisposición Genética a la Enfermedad , Humanos , Proteínas de la Membrana , Neuronas , Polimorfismo de Nucleótido Simple/genética , Sueño/genética
13.
Genetics ; 180(1): 533-45, 2008 Sep.
Artículo en Inglés | MEDLINE | ID: mdl-18757931

RESUMEN

The correlation between alleles at a pair of genetic loci is a measure of linkage disequilibrium. The square of the sample correlation multiplied by sample size provides the usual test statistic for the hypothesis of no disequilibrium for loci with two alleles and this relation has proved useful for study design and marker selection. Nevertheless, this relation holds only in a diallelic case, and an extension to multiple alleles has not been made. Here we introduce a similar statistic, R2, which leads to a correlation-based test for loci with multiple alleles: for a pair of loci with k and m alleles, and a sample of n individuals, the approximate distribution of n(k - 1)(m - 1)/(km)R2 under independence between loci is chi2(k-1(m-1). One advantage of this statistic is that it can be interpreted as the total correlation between a pair of loci. When the phase of two-locus genotypes is known, the approach is equivalent to a test for the overall correlation between rows and columns in a contingency table. In the phase-known case, R2 is the sum of the squared sample correlations for all km 2 x 2 subtables formed by collapsing to one allele vs. the rest at each locus. We examine the approximate distribution under the null of independence for R2 and report its close agreement with the exact distribution obtained by permutation. The test for independence using R2 is a strong competitor to approaches such as Pearson's chi square, Fisher's exact test, and a test based on Cressie and Read's power divergence statistic. We combine this approach with our previous composite-disequilibrium measures to address the case when the genotypic phase is unknown. Calculation of the new multiallele test statistic and its P-value is very simple and utilizes the approximate distribution of R2. We provide a computer program that evaluates approximate as well as "exact" permutational P-values.


Asunto(s)
Alelos , Desequilibrio de Ligamiento , Animales , Mapeo Cromosómico , Frecuencia de los Genes , Genotipo , Haplotipos , Humanos , Modelos Genéticos , Modelos Estadísticos , Modelos Teóricos , Método de Montecarlo , Polimorfismo de Nucleótido Simple , Programas Informáticos , Procesos Estocásticos
14.
Front Genet ; 10: 1051, 2019.
Artículo en Inglés | MEDLINE | ID: mdl-31824555

RESUMEN

We approach the problem of combining top-ranking association statistics or P-values from a new perspective which leads to a remarkably simple and powerful method. Statistical methods, such as the rank truncated product (RTP), have been developed for combining top-ranking associations, and this general strategy proved to be useful in applications for detecting combined effects of multiple disease components. To increase power, these methods aggregate signals across top ranking single nucleotide polymorphisms (SNPs), while adjusting for their total number assessed in a study. Analytic expressions for combined top statistics or P-values tend to be unwieldy, which complicates interpretation and practical implementation and hinders further developments. Here, we propose the augmented rank truncation (ART) method that retains main characteristics of the RTP but is substantially simpler to implement. ART leads to an efficient form of the adaptive algorithm, an approach where the number of top ranking SNPs is varied to optimize power. We illustrate our methods by strengthening previously reported associations of µ-opioid receptor variants with sensitivity to pain.

15.
Pain ; 160(3): 579-591, 2019 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-30431558

RESUMEN

Painful temporomandibular disorders (TMDs) are the leading cause of chronic orofacial pain, but its underlying molecular mechanisms remain obscure. Although many environmental factors have been associated with higher risk of developing painful TMD, family and twin studies support a heritable genetic component as well. We performed a genome-wide association study assuming an additive genetic model of TMD in a discovery cohort of 999 cases and 2031 TMD-free controls from the Orofacial Pain: Prospective Evaluation and Risk Assessment (OPPERA) study. Using logistic models adjusted for sex, age, enrollment site, and race, we identified 3 distinct loci that were significant in combined or sex-segregated analyses. A single-nucleotide polymorphism on chromosome 3 (rs13078961) was significantly associated with TMD in males only (odds ratio = 2.9, 95% confidence interval: 2.02-4.27, P = 2.2 × 10). This association was nominally replicated in a meta-analysis of 7 independent orofacial pain cohorts including 160,194 participants (odds ratio = 1.16, 95% confidence interval: 1.0-1.35, P = 2.3 × 10). Functional analysis in human dorsal root ganglia and blood indicated this variant is an expression quantitative trait locus, with the minor allele associated with decreased expression of the nearby muscle RAS oncogene homolog (MRAS) gene (beta = -0.51, P = 2.43 × 10). Male mice, but not female mice, with a null mutation of Mras displayed persistent mechanical allodynia in a model of inflammatory pain. Genetic and behavioral evidence support a novel mechanism by which genetically determined MRAS expression moderates the resiliency to chronic pain. This effect is male-specific and may contribute to the lower rates of painful TMD in men.


Asunto(s)
Dolor Facial/etiología , Polimorfismo de Nucleótido Simple/genética , Trastornos de la Articulación Temporomandibular/complicaciones , Trastornos de la Articulación Temporomandibular/genética , Proteínas ras/genética , Adolescente , Adulto , Anciano , Anciano de 80 o más Años , Animales , Estudios de Cohortes , Modelos Animales de Enfermedad , Estudios de Asociación Genética , Estudio de Asociación del Genoma Completo , Genotipo , Humanos , Masculino , Ratones , Ratones Noqueados , Persona de Mediana Edad , ARN Mensajero/metabolismo , Adulto Joven , Proteínas ras/deficiencia
16.
J Clin Invest ; 127(9): 3353-3366, 2017 Sep 01.
Artículo en Inglés | MEDLINE | ID: mdl-28783046

RESUMEN

The EGFR belongs to the well-studied ErbB family of receptor tyrosine kinases. EGFR is activated by numerous endogenous ligands that promote cellular growth, proliferation, and tissue regeneration. In the present study, we have demonstrated a role for EGFR and its natural ligand, epiregulin (EREG), in pain processing. We show that inhibition of EGFR with clinically available compounds strongly reduced nocifensive behavior in mouse models of inflammatory and chronic pain. EREG-mediated activation of EGFR enhanced nociception through a mechanism involving the PI3K/AKT/mTOR pathway and matrix metalloproteinase-9. Moreover, EREG application potentiated capsaicin-induced calcium influx in a subset of sensory neurons. Both the EGFR and EREG genes displayed a genetic association with the development of chronic pain in several clinical cohorts of temporomandibular disorder. Thus, EGFR and EREG may be suitable therapeutic targets for persistent pain conditions.


Asunto(s)
Dolor Crónico/metabolismo , Epirregulina/genética , Epirregulina/fisiología , Receptores ErbB/fisiología , Adolescente , Adulto , Animales , Conducta Animal , Estudios de Casos y Controles , Estudios de Cohortes , Drosophila melanogaster , Femenino , Humanos , Hiperalgesia/metabolismo , Inflamación , Ligandos , Masculino , Metaloproteinasa 9 de la Matriz/metabolismo , Ratones , Mutación , Neuronas/metabolismo , Manejo del Dolor , Fosforilación , Polimorfismo de Nucleótido Simple , Unión Proteica , Transducción de Señal , Adulto Joven
17.
18.
Genetics ; 171(2): 813-23, 2005 Oct.
Artículo en Inglés | MEDLINE | ID: mdl-16020784

RESUMEN

With the recent advances in high-throughput genotyping techniques, it is now possible to perform whole-genome association studies to fine map causal polymorphisms underlying important traits that influence susceptibility to human diseases and efficacy of drugs. Once a genome scan is completed the results can be sorted by the association statistic value. What is the probability that true positives will be encountered among the first most associated markers? When a particular polymorphism is found associated with the trait, there is a chance that it represents either a "true" or a "false" association (TA vs. FA). Setting appropriate significance thresholds has been considered to provide assurance of sufficient odds that the associations found to be significant are genuine. However, the problem with genome scans involving thousands of markers is that the statistic values of FAs can reach quite extreme magnitudes. In such situations, the distributions corresponding to TAs and the most extreme FAs become comparable and significance thresholds tend to penalize TAs and FAs in a similar fashion. When sorting between true and false associations, the "typical" place (i.e., rank) of TAs among the most significant outcomes becomes important, ordered by the association statistic value. The distribution of ranks that we study here allows calculation of several useful quantities. In particular, it gives the number of most significant markers needed for a follow-up study to guarantee that a true association is included with certain probability. This can be calculated conditionally on having applied a multiple-testing correction. Effects of multilocus (e.g., haplotype association) tests and impact of linkage disequilibrium on the distribution of ranks associated with TAs are evaluated and can be taken into account.


Asunto(s)
Enfermedades Genéticas Congénitas/genética , Predisposición Genética a la Enfermedad , Genómica/métodos , Modelos Genéticos , Polimorfismo Genético , Proyectos de Investigación , Simulación por Computador , Marcadores Genéticos/genética , Haplotipos/genética , Desequilibrio de Ligamiento
19.
Eur J Hum Genet ; 24(9): 1316-23, 2016 08.
Artículo en Inglés | MEDLINE | ID: mdl-26883092

RESUMEN

Young-onset breast cancer shows certain phenotypic and etiologic differences from older-onset breast cancer and may be influenced by some distinct genetic variants. Few genetic studies of breast cancer have targeted young women and no studies have examined whether maternal variants influence disease in their adult daughters through prenatal effects. We conducted a family-based, genome-wide association study of young-onset breast cancer (age at diagnosis <50 years). A total of 602 188 single-nucleotide polymorphisms (SNPs) were genotyped for 1279 non-Hispanic white cases and their parents or sisters. We used likelihood-based log-linear models to test for transmission asymmetry within families and for maternally mediated genetic effects. Three autosomal SNPs (rs28373882, P=2.8 × 10(-7); rs879162, P=9.2 × 10(-7); rs12606061, P=9.1 × 10(-7)) were associated with risk of young-onset breast cancer at a false-discovery rate below 0.20. None of these loci has been previously linked with young-onset or overall breast cancer risk, and their functional roles are unknown. There was no evidence of maternally mediated, X-linked, or mitochondrial genetic effects, and no notable findings within cancer subcategories defined by menopausal status, estrogen receptor status, or by tumor invasiveness. Further investigations are needed to explore other potential genetic, epigenetic, or epistatic mechanisms and to confirm the association between these three novel loci and young-onset breast cancer.


Asunto(s)
Neoplasias de la Mama/genética , Impresión Genómica , Herencia Paterna , Polimorfismo de Nucleótido Simple , Adulto , Edad de Inicio , Neoplasias de la Mama/patología , Femenino , Sitios Genéticos , Estudio de Asociación del Genoma Completo , Humanos , Masculino , Persona de Mediana Edad , Madres , Linaje , Hermanos
20.
Genetics ; 168(2): 1029-40, 2004 Oct.
Artículo en Inglés | MEDLINE | ID: mdl-15514073

RESUMEN

There has been much recent interest in describing the patterns of linkage disequilibrium (LD) along a chromosome. Most empirical studies that have examined this issue have concentrated on LD between collections of pairs of markers and have not considered the joint effect of a group of markers beyond these pairwise connections. Here, we examine many different patterns of LD defined by both pairwise and joint multilocus LD terms. The LD patterns we considered were chosen in part by examining those seen in real data. We examine how changes in these patterns affect the power to detect association when performing single-marker and haplotype-based case-control tests, including a novel haplotype test based on contrasting LD between affected and unaffected individuals. Through our studies we find that differences in power between single-marker tests and haplotype-based tests in general do not appear to be large. Where moderate to high levels of multilocus LD exist, haplotype tests tend to be more powerful. Single-marker tests tend to prevail when pairwise LD is high. For moderate pairwise values and weak multilocus LD, either testing strategy may come out ahead, although it is also quite likely that neither has much power.


Asunto(s)
Biología Computacional/estadística & datos numéricos , Variación Genética/genética , Desequilibrio de Ligamiento/genética , Polimorfismo Genético/genética , Carácter Cuantitativo Heredable , Estudios de Casos y Controles , Biología Computacional/métodos , Marcadores Genéticos/genética , Genotipo , Haplotipos , Humanos , Fenotipo
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