RESUMEN
Two new ruthenium complexes with [Ru(L)(2)(qip)](2+) (L=bpy (2,2'- bipyridine), phen (1,10-phenanthroline); qip=2-(2-quinolinyl)imidazo[4,5-f][1,10]phenanthroline), have been synthesized and characterized by elemental analysis, ES-MS, (1)H NMR. The binding properties of two complexes towards CT-DNA were investigated by various optical methods and viscosity measurements. The experiment results suggested that both Ru(II) complexes can intercalate into DNA base pairs. Strong quenching in emission intensity of two Ru(II) complexes were observed upon addition of Ag(+) in the absence and presence of CT-DNA. Furthermore, the two complexes can promote cleavage of pBR322 DNA under irradiation at 365 nm, and complex 2 exhibits a stronger DNA-photocleavage efficiency than complex 1. The mechanism of DNA cleavage suggests that singlet oxygen ((1)O(2)) is likely to be the cleaving agent.
Asunto(s)
2,2'-Dipiridil/análogos & derivados , ADN/metabolismo , Sustancias Intercalantes/química , Sustancias Intercalantes/farmacología , Compuestos Organometálicos/química , Compuestos Organometálicos/farmacología , Fenantrolinas/química , Fenantrolinas/farmacología , 2,2'-Dipiridil/química , 2,2'-Dipiridil/farmacología , Animales , Bovinos , ADN/química , División del ADN/efectos de los fármacos , División del ADN/efectos de la radiación , Imidazoles/química , Imidazoles/farmacología , Mediciones Luminiscentes , Procesos Fotoquímicos , Plata/metabolismoRESUMEN
A novel asymmetric ligand 2-(pyridine-2-yl)-1-H-imidazo[4,5-i]dibenzo[2,3-a:2',3'-c]phenazine (pidbp) and its ruthenium complexes [Ru(L)(2)(pidbp)](2+) (L=bpy (2, 2'- bipyridine), phen (1, 10 - phenanthroline)), have been synthesized and characterized by elemental analysis, ES-MS, (1)H NMR. Various methods support the conclusion that both Ru(II) complexes can intercalate into DNA base pairs. Complex [Ru(bpy)(2)(pidbp)](2+)4 exhibits its DNA "molecular light switch" properties. Furthermore, the two complexes are efficient DNA-photocleavers under irradiation at 365 nm, and complex 5 exhibits a stronger DNA-photocleavage efficiency than complex 4. The mechanism of DNA cleavage is an oxidative process by generating singlet oxygen.
Asunto(s)
Complejos de Coordinación/metabolismo , División del ADN/efectos de la radiación , ADN/metabolismo , Sustancias Intercalantes/metabolismo , Rutenio/metabolismo , Absorción/efectos de la radiación , Complejos de Coordinación/síntesis química , Complejos de Coordinación/química , Electrones , Electroforesis en Gel de Agar , Etidio/química , Sustancias Intercalantes/química , Ligandos , Luz , Plásmidos/metabolismo , Rutenio/química , Espectrometría de Fluorescencia , Volumetría , Viscosidad/efectos de la radiaciónRESUMEN
Two novel Ru(II) complexes [Ru(bpy)(2)(pyip)](2+)1 and [Ru(phen)(2)(pyip)](2+)2 (bpy=2,2'-bipyridine; phen=1,10-phenanthroline; pyip=2-(pyridine-2-yl)imidazo-[4,5-f][1,10]-phenanthroline), have been synthesized and characterized by elemental analysis, ES-MS, (1)H NMR, UV-Vis. The DNA-binding behaviors of both complexes were studied by spectroscopic methods and viscosity measurements. The results indicate that the two complexes can bind to CT-DNA in an intercalative mode, and also show that these two Ru(II) complexes can promote the photocleavage of pBR322 DNA. In addition, In the presence of Co(2+), the emission of DNA-[Ru(L)(2)pyip](2+) can be quenched, which exhibited the DNA "light switch" properties.