Effect of various phosphorus levels on the extraction of Cd, the transformation of P, and phosphorus-related gene during the phytoremediation of Cd contaminated soil.

Phytoremediation has emerged as a common technique for remediating Cd pollution in farmland soil. Moreover, phosphorus, an essential element for plants, can alter the pectin content of plant cell walls and facilitate the accumulation of Cd in plant tissues, thereby enhancing phytoremediation efficiency. Therefore, pot experiments were conducted in order to investigate the effect of phosphorus levels on Cd extraction, phosphorus transformation and phosphorus-related genes during phytoremediation. The results revealed that an optimal application of suitable phosphate fertilizers elevated the soil's pH and electrical conductivity (EC), facilitated the conversion of soil from insoluble phosphorus into available forms, augmented the release of pertinent enzyme activity, and induced the expression of phosphorus cycling-related genes. These enhancements in soil conditions significantly promoted the growth of ryegrass. When applying phosphorus at a rate of 600 mg/kg, ryegrass exhibited plant height, dry weight, and chlorophyll relative content that were 1.27, 1.26, and 1.18 times higher than those in the control group (P0), while the Cd content was 1.12 times greater than that of P0. The potentially toxic elements decline ratio and bioconcentration factor were 42.86% and 1.17 times higher than those of P0, respectively. Consequently, ryegrass demonstrated the highest Cd removal efficiency under these conditions. Results from redundancy analysis (RDA) revealed a significant correlation among pH, total phosphorus, heavy metal content, phosphorus forms, soil enzyme activity, and phosphorus-related genes. In conclusion, this study suggests applying an optimal amount of suitable phosphate fertilizers can enhance restoration efficiency, leading to a reduction in soil Cd content and ultimately improving the safety of crop production in farmlands.

Effect of the Combination of Phosphate-Solubilizing Bacteria with Orange Residue-Based Activator on the Phytoremediation of Cadmium by Ryegrass.

Amendments with activators or microorganisms to enhance phytoremediation in toxic-metal-polluted soils have been widely studied. In this research, the production of indoleacetic acid, siderophore, and 1-aminocyclopropane-1-carboxylate (ACC) deaminase by phosphate-solubilizing bacteria was investigated during a pure culture experiment. Pot experiments were performed using Cd-polluted soil with the following treatments: control (CK, only ultrapure water), orange-peel-based activator (OG), and a combination of phosphate-solubilizing bacteria (Acinetobacter pitti) and OG (APOG). Ryegrass plant height and fresh weight, Cd content in ryegrass, total and available Cd soil content, soil enzyme activity, and soil bacterial diversity were determined in this work. The findings showed that the height of ryegrass in OG and APOG increased by 14.78% and 21.23%. In the APOG group, a decreased ratio of Cd was 3.37 times that of CK, and the bioconcentration factor was 1.28 times that of CK. The neutral phosphatase activity of APOG was 1.33 times that of CK and catalase activity was 1.95 times that of CK. The activity of urease was increased by 35.48%. APOG increased the abundance of beneficial bacteria and Proteobacteria was the dominant bacterium, accounting for 57.38% in APOG. Redundancy analysis (RDA) showed that nutrient elements were conducive to the propagation of the dominant bacteria, the secretion of enzymes, and the extraction rate of Cd in the soil. The possible enhancement mechanism of phytoremediation of cadmium by A. pitti combined with OG was that, on the one hand, APOG increased soil nutrient elements and enzyme activities promoted the growth of ryegrass. On the other hand, APOG activated Cd and boosted the movement of Cd from soil to ryegrass. This research offers insight for the combination of phosphate-solubilizing bacteria with an orange-peel-based activator to improve phytoremediation of Cd-contaminated soils and also provides a new way for the resource utilization of fruit residue.

Effect of different amounts of fruit peel-based activator combined with phosphate-solubilizing bacteria on enhancing phytoextraction of Cd from farmland soil by ryegrass.

To improve the uptake of heavy metals by plants and increase the effectiveness of phytoextraction, chelating agents are employed to change the speciation of heavy metals in soil and increase their bioavailability. However, the effect of a single activator is limited. In recent years, compound activators have been applied widely to improve phytoextraction efficiency. In this study, a fruit peel-based activator (OG) was prepared, containing a mixture of orange peel extracts and tetrasodium glutamate diacetate (GLDA) (1.6% v/v) in a ratio of 1:1 (v/v). The pot experiment was used to investigate the effects of different amounts of OG combined with phosphate-solubilizing bacteria (Acinetobacter pitti, AP) on the extraction of Cd from farmland soil by ryegrass (Lolium perenne L). The results indicated that the addition of OG and AP increased the pH and EC of the soil and improved the content of nutrient elements in the soil. The optimal combination of the application rates of OG and AP improved the growth of ryegrass and enhanced the phytoextraction of Cd. Redundancy analysis (RDA) showed that total soil nitrogen had the greatest influence on phytoextraction, with a contribution rate of 85.3%, followed by pH, with a contribution rate of 7.7%. Total nitrogen, pH, available phosphorus, alkaline nitrogen, and total organic matter were correlated positively with plant Cd, soil Cd decrease ratio, and the bioaccumulation factor but negatively with total Cd and available Cd. Based on the findings of this study, it is feasible to apply the fruit peel-based activator (amended with GLDA) and phosphate-solubilizing bacteria to enhance phytoextraction of Cd, which will provide a valuable reference for the treatment of heavy metal-contaminated soils and the reutilization of fruit peel waste. When applying the compound activator, it is recommended to consider the influence of the additional amount of compound activator on the extraction efficiency.