RESUMEN
OBJECTIVE: To develop a method to measure trihexyphenidyl, chlorpromazine and clozapine in human blood with GC-MS. METHODS: The specimens were alkalized (pH > 10) and extracted with V (benzene):V(ethyl acetate) = 1:1, and qualitatively analyzed using GC-MS-Full Scan with internal standard SKF525A. The specimens were alkalized (pH > 10) and extracted with V(benzene):V(ethyl acetate) = 1:1, and quantitatively analyzed using GC-MS-SIM with internal standard diazepam-d5. RESULTS: The lowest detection limits of trihexyphenidyl, chlorpromazine and clozapine were 0.3, 0.3 and 0.7 ng/mL (S/N > or = 3) respectively. The calibration curve in 20-10 000 ng/mL showed a good linear distribution. The recovery rate was 79.9% to 85.5%. The RSDs of intraday and interday were less than 5.1%. CONCLUSION: The established method was simple, sensitive and accurate for simultaneous determination of trihexyphenidyl, chlorpromazine and clozapine in human blood, and can be applied in forensic toxicological cases.
Asunto(s)
Antipsicóticos/sangre , Clorpromazina/sangre , Clozapina/sangre , Cromatografía de Gases y Espectrometría de Masas/métodos , Trihexifenidilo/sangre , Adulto , Antipsicóticos/envenenamiento , Femenino , Toxicología Forense , Humanos , Concentración de Iones de Hidrógeno , Masculino , Persona de Mediana Edad , Reproducibilidad de los Resultados , Sensibilidad y Especificidad , Solventes/químicaRESUMEN
OBJECTIVE: To study the chemical components from dried roots of Zanthoxylum dimorphophyllum var. spinifoliun. METHOD: The chemical components were isolated by low pressure column chromatography and their structures were identified by spectroscopic methods. RESULT: Five compounds were isolated and identified as 6-(2', 3'-dihydroxy-3'-methyl-butyl)-7-hydroxy-5-methoxy-2H-1-benzopyran-2-one (I), 6-(2',3'-dihydroxy-3'-methyl-butyl)-7-methoxy-8-(3'-methyl-but-2'-enyl)-2H-1-benzopyran-2-one (II),6-(2',3'-dihydroxy-3'-methyl-butyl)-7-hydroxy-2H-1-benzopyran-2-one (III), 6-(2', 3'-oxiranyl-3'-methyl-butyl)-7-methoxy-8- (3-methyl-but-2-enyl)-2H-1-benzopyran-2-one (IV), 7-methoxy-8-(3'-methyl-but-2'-enyl)-2H-1-benzopyran-2-one (V). CONCLUSION: These compounds were isolated from the plant for the first time.
Asunto(s)
Cumarinas/aislamiento & purificación , Plantas Medicinales/química , Zanthoxylum/química , Cumarinas/química , Raíces de Plantas/químicaRESUMEN
OBJECTIVE: To study the chemical constituents of the dried roots of Zanthoxylum dimorphophyllumr. spinifolium and to find out the active constituents of the plant. METHOD: Modern chromatography was used to purify chemical constituents, and their structures were identified by various spectral methods. RESULT: Four compounds were isolated and identified as isopimpinellin (I), xanthoxyletin (II), 6-(2', 3'-dihydroxy-3'-methyl-butyl)-7-hydroxy-2H-1-benzopyran-2-one (III), 6-(2'-O-beta-D-glucopyranosyloxy, 3'-dihydroxy-3'-methybutyl)-7-hydroxy-2H-1-benzopyran-2-one (IV). CONCLUSION: All of the above compounds were isolated from the above mentioned plant for the first time.