Enhancing the prediction of symptomatic radiation pneumonitis for locally advanced non-small-cell lung cancer by combining 3D deep learning-derived imaging features with dose-volume metrics: a two-center study.

OBJECTIVE: This study aims to examine the ability of deep learning (DL)-derived imaging features for the prediction of radiation pneumonitis (RP) in locally advanced non-small-cell lung cancer (LA-NSCLC) patients. MATERIALS AND METHODS: The study cohort consisted of 90 patients from the Fudan University Shanghai Cancer Center and 59 patients from the Affiliated Hospital of Jiangnan University. Occurrences of RP were used as the endpoint event. A total of 512 3D DL-derived features were extracted from two regions of interest (lung-PTV and PTV-GTV) delineated on the pre-radiotherapy planning CT. Feature selection was done using LASSO regression, and the classification models were built using the multilayered perceptron method. Performances of the developed models were evaluated by receiver operating characteristic curve analysis. In addition, the developed models were supplemented with clinical variables and dose-volume metrics of relevance to search for increased predictive value. RESULTS: The predictive model using DL features derived from lung-PTV outperformed the one based on features extracted from PTV-GTV, with AUCs of 0.921 and 0.892, respectively, in the internal test dataset. Furthermore, incorporating the dose-volume metric V30Gy into the predictive model using features from lung-PTV resulted in an improvement of AUCs from 0.835 to 0.881 for the training data and from 0.690 to 0.746 for the validation data, respectively (DeLong p < 0.05). CONCLUSION: Imaging features extracted from pre-radiotherapy planning CT using 3D DL networks could predict radiation pneumonitis and may be of clinical value for risk stratification and toxicity management in LA-NSCLC patients. CLINICAL RELEVANCE STATEMENT: Integrating DL-derived features with dose-volume metrics provides a promising noninvasive method to predict radiation pneumonitis in LA-NSCLC lung cancer radiotherapy, thus improving individualized treatment and patient outcomes.

In Situ Growth of Highly Compatible Cu2O-GO Hybrids via Amino-Modification for Melt-Spun Efficient Antibacterial Polyamide 6 Fibers.

Polyamide 6 fiber has the advantages of high strength and good wear resistance. However, it is still challenging to effectively load inorganic antibacterial agents into polymer substrates without antimicrobial activity. In this work, graphene oxide was used as a carrier, which was modified with an aminosilane coupling agent (AEAPTMS) to enhance the compatibility and antimicrobial properties of the inorganic material, as well as to improve its thermal stability in a high-temperature melting environment. Cuprous oxide-loaded aminated grapheme (Cu2O-GO-NH2) was constructed by in situ growth method, and further PA6/Cu2O-GO-NH2 fibers were prepared by in situ polymerization. The composite fiber has excellent washing resistance. After 50 times washing, its bactericidal rates against Bacillus subtilis and Escherichia coli were 98.85% and 99.99%, respectively. In addition, the enhanced compatibility of Cu2O-GO-NH2 with the PA6 matrix improved the orientation and crystallinity of the composite fibers. Compared with PA6/Cu2O-GO fibers, the fracture strength of PA6/Cu2O-GO-NH2 fibers increased from 3.0 cN/dtex to 4.2 cN/dtex when the addition of Cu2O-GO-NH2 was 0.2 wt.%. Chemical modification and in situ concepts help to improve the compatibility of inorganic antimicrobial agents with organic polymers, which can be applied to the development of medical textiles. This article is protected by copyright. All rights reserved.

High incidence of EDNRB gene mutation in seven southern Chinese familial cases with Hirschsprung's disease.

PURPOSE: Hirschsprung's disease (HSCR) is the leading cause of neonatal functional intestinal obstruction, which has been identified in many familial cases. HSCR, a multifactorial disorder of enteric nervous system (ENS) development, is associated with at least 24 genes and seven chromosomal loci, with RET and EDNRB as its major genes. We present a genetic investigation of familial HSCR to clarify the genotype-phenotype relationship. METHODS: We performed whole exome sequencing (WES) on Illumina HiSeq X Ten platform to investigate genetic backgrounds of core family members, and identified the possibly harmful mutation genes. Mutation carriers and pedigree relatives were validated by Sanger sequencing for evaluating the gene penetrance. RESULTS: Four familial cases showed potential disease-relative variants in EDNRB and RET gene, accounting for all detection rate of 57.1%. Three familial cases exhibited strong pathogenic variants as frameshift or missense mutations in EDNRB gene. A novel c.367delinsTT mutation of EDNRB was identified in one family member. The other two EDNRB mutations, c.553G>A in family 2 and c.877delinsTT in family 5, have been reported in previous literatures. The penetrance of EDNRB variants was 33-50% according mutation carries. In family 6, the RET c.1858T>C (C620R) point mutation has previously been reported to cause HSCR, with 28.5% penetrance. CONCLUSION: We identified a novel EDNRB (deleted C and inserted TT) mutation in this study using WES. Heterozygote variations in EDNRB gene were significantly enriched in three families and RET mutations were identified in one family. EDNRB variants showed an overall higher incidence and penetrance than RET in southern Chinese families cases.

A Dragon Fruit Picking Detection Method Based on YOLOv7 and PSP-Ellipse.

Dragon fruit is one of the most popular fruits in China and Southeast Asia. It, however, is mainly picked manually, imposing high labor intensity on farmers. The hard branches and complex postures of dragon fruit make it difficult to achieve automated picking. For picking dragon fruits with diverse postures, this paper proposes a new dragon fruit detection method, not only to identify and locate the dragon fruit, but also to detect the endpoints that are at the head and root of the dragon fruit, which can provide more visual information for the dragon fruit picking robot. First, YOLOv7 is used to locate and classify the dragon fruit. Then, we propose a PSP-Ellipse method to further detect the endpoints of the dragon fruit, including dragon fruit segmentation via PSPNet, endpoints positioning via an ellipse fitting algorithm and endpoints classification via ResNet. To test the proposed method, some experiments are conducted. In dragon fruit detection, the precision, recall and average precision of YOLOv7 are 0.844, 0.924 and 0.932, respectively. YOLOv7 also performs better compared with some other models. In dragon fruit segmentation, the segmentation performance of PSPNet on dragon fruit is better than some other commonly used semantic segmentation models, with the segmentation precision, recall and mean intersection over union being 0.959, 0.943 and 0.906, respectively. In endpoints detection, the distance error and angle error of endpoints positioning based on ellipse fitting are 39.8 pixels and 4.3°, and the classification accuracy of endpoints based on ResNet is 0.92. The proposed PSP-Ellipse method makes a great improvement compared with two kinds of keypoint regression method based on ResNet and UNet. Orchard picking experiments verified that the method proposed in this paper is effective. The detection method proposed in this paper not only promotes the progress of the automatic picking of dragon fruit, but it also provides a reference for other fruit detection.

Two Novel, Nonoperative Methods of Stoma Prolapse in Newborns: 3 Case Reports.

BACKGROUND: Prolapse is a common complication following enterostomy; the defect and consequences of a prolapse significantly affect health-related quality of life. Creative techniques must be employed to manage the prolapse. CASES: This article describes management of 3 neonates with stoma prolapse. CONCLUSION: Management of stoma prolapse should be individualized, employing successful nonoperative techniques rather than more difficult operative procedures to prevent recurrent prolapse.

Enhanced molecular binding affinity toward aromatic dications by anthracene-derived crown ethers in water.

The pursuit of high molecular binding affinity using conventional crown ethers in water remains a challenging task in the field of supramolecular chemistry and may hold great promise in the creation of advanced biocompatible nanoconstructs. In this work, the molecular binding strength toward a series of structurally relevant cationic guests has been greatly enhanced by tetrasulfonated 1,5-dianthracenyl-42-crown-10 and as investigated by means of 1H NMR, UV-vis, and fluorescence spectroscopy, the host-guest association constants can reach up to 108 M-1 order of magnitude in aqueous solution. X-ray crystal diffraction analysis further demonstrates that the aromatic dication can be tightly encapsulated in the ring of anthracene-derived crown ether via multiple π-stacking and electrostatic interactions. Meanwhile, the obtained association constants are remarkably higher than the ones in the cases of the known benzene- and naphthalene-derived sulfonated crown ethers, substantiating that the appropriate extension of π-conjugation in the molecular skeleton of crown ether is a feasible method in attaining a highly affiliative host-guest complex. Taken together, our results indicate that the anthracene-based sulfonated crown ether can be developed as a new family of water-soluble macrocyclic receptors in the fabrication of functional nanoarchitectures.

Knockdown of MAGE-A6 enhanced the irradiation sensitivity of non-small cell lung cancer cells by activating the AMPK pathway.

Non-small cell lung cancer is a common respiratory tumor. The mortality rate of lung cancer patients has continued to rise in recent years. Several studies revealed that the expression of melanoma antigen 6 (MAGE-A6) promoted the development of multiple types of cancer. In addition, the suppression of AMPK pathway could restrict the radiosensitization of prostate cancer cells. Inhibition of MAGE-A6 activated the AMPK pathway in colorectal cancer cells. However, whether the MAGE-A6 could regulate the radiosensitivity of non-small cell lung cancer cells by regulating of the AMPK pathway is unclear. In this study, we established the MAGE-A6 knockdown in A549 and H1299 cells. Next, the apoptosis and proliferation of these cells were detected by the flow cytometry analysis and colony formation assay after the irradiation, respectively. Then, the expression of p-AMPKα1 and p-S6K1 in these cells was explored by the western blotting. After that, we inhibited the expression of AMPKα1 in MAGE-A6 knockdown cells. The proliferation and apoptosis of these cells were detected with colony formation assay and flow cytometry analysis. Finally, the tumor formation of these cells was detected in nude mice. Our results showed that inhibition of MAGE-A6 suppressed the proliferation and aggravated the apoptosis of A549 and H1299 cells after the irradiation. Knockdown of MAGE-A6 activated the expression of p-AMPKα1 and repressed the expression of p-S6K1 in these cells. Suppression of AMPKα1 in MAGE-A6 knockdown cells abolished these effects. Knockdown of MAGE-A6 also enhanced the radiosensitivity of these cells in vivo. These results suggested that inhibition of MAGE-A6 promoted the radiosensitivity of non-small cell lung cancer cells by activating AMPK pathway. Therefore, MAGE-6 has the potential to be explored as the therapeutic target for the treatment of non-small cell lung cancer in clinical.

Wnt5a-Ror2 signaling mediates root resorption.

INTRODUCTION: This study aimed to investigate the role of wingless-type MMTV integration site family member 5a (Wnt5a)-receptor tyrosine kinase-like orphan receptor 2 (Ror2) signaling in root resorption. METHODS: The messenger RNA (mRNA) expression of Wnt5a, Ror2, and RANKL in periodontal ligament cells (PDLCs) under compression force (CF) with or without Ror2 small interfering RNA (siRNA) were measured by quantitative reverse transcription-polymerase chain reaction, and these proteins released into culture supernatants were measured using enzyme-linked immunosorbent assay. Then these PDLC-conditioned media under CF with or without Ror2 siRNA were used to culture osteoclast precursors to detect osteoclastogenesis effects via tartrate-resistant acid phosphatase staining. In in vivo studies, the odontoclast number and the root resorption volume under excessive CF with or without Ror2 siRNA were investigated by tartrate-resistant acid phosphatase immunohistochemical staining and microcomputed tomography. The protein levels for Wnt5a, Ror2, and receptor activator of nuclear factor-kappa B ligand (RANKL) in the periodontal ligament tissues were also detected using immunohistochemical staining. Finally, the odontoclast number, root resorption volume, and the mRNA and protein expressions were compared between immature and mature teeth. RESULTS: The mRNA production and protein release level of Wnt5a, Ror2, and RANKL increased after CF, whereas they were significantly downregulated with Ror2 siRNA. The osteoclast number increased treating with culture medium from PDLC applying CF, but the increase was inhibited after adding Ror2 siRNA. In the animal model, the odontoclast number and root resorption volume significantly increased in the CF group but decreased in the CF with the Ror2 siRNA group. The protein levels of Wnt5a, Ror2, and RANKL in periodontal ligament were upregulated under excessive CF, and the pathway was inhibited with Ror2 siRNA. In the immature tooth group, the odontoclast number, root resorption volume, and the mRNA and protein expressions of Wnt5a-Ror2 signaling were reduced. CONCLUSIONS: Wnt5a-Ror2 signaling in PDLCs enhanced by excessive CF could promote RANKL release and induce precursor differentiation, partly leading to increased odontoclast activity and ultimate root resorption. The less resorption of the immature tooth may be due to odontoclastogenesis inhibition by decreased expression of Wnt5a-Ror2 signaling.

Thermal Stability of Bio-Based Aliphatic-Semiaromatic Copolyester for Melt-Spun Fibers with Excellent Mechanical Properties.

Flexible aliphatic poly(lactic acid) is introduced into polyethylene terephthalate through copolymerization to prepare biodegradable copolyester, which aims to solve the non-degradability of polyethylene terephthalate (PET) and realize the greening of raw materials. In this work, poly(ethylene terephthalate-co-lactic acid) random copolyesters (PETLAs) of lactic acid composition from 10 to 50% is synthesized via one-pot method. The chemical structure and composition, thermal property, and crystallization property of prepared PETLAs resin are characterized. The results shows that the introduction of LA segment forms random copolyester, and the flexible LA segment results in slight decrease in the glass transition temperatures (Tg ), melting point (Tm ), and crystallinity (Xc ) of the copolyesters. The thermal stability of PETLAs is better, and the initial decomposition temperature of PETLA-10 can reach 394 °C. The PETLAs resin exhibits good processability, and PETLAs fibers are prepared by melt spinning. The strength of PETLA-10 fiber can reach 260 MPa after drawing treatment, and the elongation at break can reach 130%. Taking advantage of their features, PETLAs as an innovative bio-based polymer are expected to achieve ecofriendly applications in the fields of fiber, plastic, and film.

Lead stress affects the reproduction of Spodoptera litura but not by regulating the vitellogenin gene promoter.

Lead (Pb) stress affects hormone-mediated responses (e.g., reproduction) in insects. In this study, the effects of Pb stress (12.5-50 mg Pb/kg in larval artificial diets) on the reproduction of the common cutworm Spodoptera litura (Lepidoptera: Noctuidae) were investigated after 7 generations. The results showed that Pb stress did not reduce the longevity of adult females, but 50 mg Pb/kg significantly reduced the longevity of adult males, regardless of the generation. After 50 mg Pb/kg stress for one or 7 generations, the peak time of egg-laying was delayed, and egg production and hatchability were decreased significantly. The vitellin content in eggs was significantly inhibited by Pb stress. The S. litura vitellogenin (Vg) gene promoter was cloned and analyzed. Multiple putative transcription factors were predicted for the 2321 bp Vg promoter region, including the TATA box, GATA, basic helix-loop-helix (bHLH) transcription factor, Broad-Complex (BR-C) binding sites, etc. The fragment from -2222 to -211 bp of the Vg promoter was the activation domain for Vg, whereas the region from -211 to -55 bp repressed the activity of the Vg promoter. The construct promoter (-782/+76) in Trichoplusia ni (Hi5) cells significantly improved Vg expression, which was not affected by Pb stress (1 or 10 mg/ml). Therefore, Pb stress significantly inhibited the reproduction of S. litura but not by regulating the Vg promoter.

New sonographic feature (C-sign) to improve the prenatal accuracy of jejunal atresia.

OBJECTIVES: To describe a new sonographic feature of the C-sign for prenatal diagnosis of jejunal atresia and evaluate its role in prenatal jejunal atresia, particularly preceding bowel dilatation and polyhydramnios. METHODS: This was a retrospective study from a tertiary maternal hospital. Patients with prenatal sonographic examination and confirmed small bowel atresia postdelivery were included. All sonographic images were reviewed by two senior sonographers. Comparison of sonographic images between prenatal jejunal and ileal atresia using the C-sign resembles the shape of the entire duodenum and other traditional sonographic features. The control group without bowel atresia was assessed for the presence of the C-sign. RESULTS: The C-sign and combined bowel dilatation with polyhydramnios were more frequent in jejunal atresia than ileal atresia, but the C-sign can be used to detect jejunal atresia earlier. The C-sign can be more likely to diagnose jejunal atresia in persisting bowel dilatation and polyhydramnios. The C-sign was not reported in any of the control fetuses. CONCLUSION: The C-sign is a new sonographic feature that can be used to improve the prenatal accuracy and early detection of jejunal atresia. However, further prospective validation is needed.

A novel three-dimensional morphological analysis of idiopathic condylar resorption following stabilisation splint treatment.

Bone modelling evaluation is important for monitoring idiopathic condylar resorption (ICR) progress. To compare condylar modelling in ICR patients treated with or without stabilisation splints (SSs). Eighty-four condyles from 84 ICR patients were studied: 42 received SS therapy (SS group); 42 received conventional therapy (control group). Cone-beam computed tomography images at diagnosis (T0) and after at least 6 months (T1) were used for three-dimensional reconstruction. Volume differences between T0 and T1 (δV) were used to evaluate the amount of modelling. Percentage of growth area (PCT) was used to assess the condylar surface growth tendency. No significant change in condylar volume was found in the SS group, whereas that in the control group was significantly decreased at T1 (P <.0001). The amount of modelling differed among condylar subregions within the SS group: among 6 subregions (P =.0137), between anterior and posterior regions (P =.0336) and between lateral, intermediate and medial regions (P =.0275). Control group condylar subregions showed no significant differences in the amount of modelling. The anabolic modelling tendency of the total condylar surface in the SS group was greater than that in the control group (P =.0251); however, there were no statistical differences in PCTs among condylar subregions in either group. SS therapy effectively reduced further bone destruction and promoted condylar modelling. Three-dimensional morphological analysis is a novel method that can accurately evaluate the amount of bone modelling and growth tendency in ICR patients.

MIR22HG acts as a tumor suppressor via TGFß/SMAD signaling and facilitates immunotherapy in colorectal cancer.

BACKGROUND: Long noncoding RNAs (lncRNAs) are emerging as critical regulatory elements and play fundamental roles in the biology of various cancers. However, we are still lack of knowledge about their expression patterns and functions in human colorectal cancer (CRC). METHODS: Differentially expressed lncRNAs in CRC were identified by bioinformatics screen and the level of MIR22HG in CRC and control tissues were determined by qRT-PCR. Cell viability and migration capacities were examined by MTT and transwell assay. Mouse model was used to examine the function and rational immunotherapy of MIR22HG in vivo. RESULTS: We systematically investigated the expression pattern of lncRNAs and revealed MIR22HG acts as a tumor suppressor in CRC. The expression of MIR22HG was significantly decreased in CRC, which was mainly driven by copy number deletion. Reduced expression of MIR22HG was significantly associated with poor overall survival. Silencing of MIR22HG promoted cell survival, proliferation and tumor metastasis in vitro and in vivo. Mechanistically, MIR22HG exerts its tumor suppressive activity by competitively interacting with SMAD2 and modulating the activity of TGFß pathway. Decreased MIR22HG promoted the epithelial-mesenchymal transition in CRC. Importantly, we found that MIR22HG expression is significantly correlated with CD8A and overexpression of MIR22HG triggers T cell infiltration, enhancing the clinical benefits of immunotherapy. CONCLUSION: MIR22HG acts as a tumor suppressor in CRC. Our data provide mechanistic insights into the regulation of MIR22HG in TGFß pathway and facilitates immunotherapy in cancer.

Spatial-temporal evolution and peer effects of urban green development efficiency in China.

In the process of global urban development, there are urgent ecological security and environmental pollution problems, green development is the fundamental way for urban sustainable development, economic transformation and mitigation of ecological and environmental problems. Based on the panel data of 283 cities at prefecture level and above in China from 2003 to 2017, this paper analyzes spatial-temporal evolution characteristics of urban green development efficiency (UGDE) and the peer effects of UGDE between cities of different grades. It is found that during the study period, in terms of temporal evolution, the average UGDE in China increased from 0.47 in 2003 to 0.61 in 2017, with a cumulative growth rate of 29.79%, showing a rising trend in general. In terms of spatial evolution, the number of low-efficiency cities and medium-efficiency cities continued to decrease. The eastern region has always been the main distribution area of higher-efficiency cities and high-efficiency cities; in the central region, UGDE in most cities improved significantly; in the western region, UGDE has always lagged behind that in the eastern and central regions. In addition, the center of gravity of UGDE presented a trend of northwest migration in general, with a total displacement of 100.07 km, and UGDE showed a spatial dispersion trend. The empirical results indicate that the improvement of UGDE in large cities has a driving effect on that in neighboring medium cities and small cities through the positive peer effect, and the growth of UGDE in medium cities has a promoting effect on that in neighboring small cities through the positive peer effect; the increase of UGDE in medium cities has a positive peer effect on that in neighboring large cities, and the growth of UGDE in small cities has a positive peer effect on that in neighboring medium cities; UGDE promotes each other between large cities through the positive peer effect.

Revisiting the hydrological legacy of revegetation on China's Loess Plateau using Eagleson's ecohydrological perspective.

Revegetation has resulted in a trend of increasing vegetation greenness on the Chinese Loess Plateau. However, it remains unclear whether the regional vegetation coverage exceeds hydroclimatic limitations in the context of revegetation, and the hydrological effects of greening are controversial. Eagleson's optimality hypothesis can explain some of the hydrological effects on the Loess Plateau. Here, building on previous research, the geospatial vegetation states were estimated for pre- and post-revegetation periods on the Loess Plateau from 1982 to 2015 using Eagleson's ecological optimality theory. Additionally, a drought composite analysis approach was utilized to investigate the hydrological effects related to drought (including sensitivity and partitioning) under various vegetation states. It was found that revegetation increased the proportion of catchments in the equilibrium state and decreased the proportion in the disturbed state, owing to a wetter climate compared with the pre-revegetation period. Root-zone soil drought, driven by precipitation (P) deficit, asymmetrically triggered hydrological effects for both the pre- and post-revegetation periods, with reduced runoff (Q) for both periods and a decrease in evapotranspiration (ET) during the pre-revegetation period but an increase in ET during the post-revegetation period. Moreover, catchments in an equilibrium state exhibited lower sensitivity between ET and P, and more stable partitioning of ET with regards to P, compared with those in a disturbed state. These results underscore the theoretical framework that an equilibrium state is crucial for maintaining ecosystem ET. Our results highlight the necessity of considering the hydrologic regulation of vegetation states when assessing the hydrological effects of vegetation change.

A Flexible Long-Wave Infrared Radiation Modulator Integrated with Electrochromic Behavior for Dual-Band Camouflage.

Electrochromic devices (ECDs), which are capable of modulating optical properties in the visible and long-wave infrared (LWIR) spectra under applied voltage, are of great significance for military camouflage. However, there are a few materials that can modulate dual frequency bands. In addition, the complex and specialized structural design of dual-band ECDs poses significant challenges. Here, we propose a novel approach for a bendable ECD capable of modulating LWIR radiation and displaying multiple colors. Notably, it eliminates the need for a porous electrode or a grid electrode, thereby improving both the response speed and fabrication feasibility. The device employs multiwalled carbon nanotubes (MWCNTs) as both the transparent electrode and the LWIR modulator, polyaniline (PANI) as the electrochromic layer, and ionic liquids (HMIM[TFSI]) as the electrolyte. The ECD is able to reduce its infrared emissivity (Δε = 0.23) in a short time (resulting in a drop in infrared temperature from 50 to 44 °C) within a mere duration of 0.78 ± 0.07 s while changing its color from green to yellow within 3 s when a positive voltage of 4 V is applied. In addition, it exhibits excellent flexibility, even under bending conditions. This simplified structure provides opportunities for applications such as wearable adaptive camouflage and multispectral displays.

Ruscogenin Attenuates Ulcerative Colitis in Mice by Inhibiting Caspase-1-Dependent Pyroptosis via the TLR4/NF-κB Signaling Pathway.

Inflammatory bowel diseases (IBD) are chronic inflammatory disorders affecting the digestive tract, including ulcerative colitis and Crohn's disease. Ruscogenin, a prominent steroidal sapogenin present in radix ophiopogon japonicus, has shown a protective effect on attenuating the inflammatory response associated with inflammatory diseases, but the efficacy of ruscogenin in IBD remains unclear. The aim of this study is to explore the effect of ruscogenin on intestinal barrier dysfunction and inflammatory responses as well as the underlying mechanism in ulcerative colitis. A dextran sulfate sodium salt (DSS)-induced C57BL/6 mouse colitis model was employed for the in vivo studies, while in vitro experiments were performed in THP-1 cells and human intestinal epithelial cells involved in inducing inflammatory responses and pyroptosis using LPS/nigericin. The results indicated that ruscogenin treatment attenuated the symptoms of ulcerative colitis, reduced the release of inflammatory cytokines and the expression of pyroptosis-associated proteins, and restored the integrity of the intestinal epithelial barrier in colon tissue in mice. Moreover, ruscogenin inhibited LPS/nigericin-induced pyroptosis in THP-1 cells. Mechanically, ruscogenin inhibited NLRP3 inflammasome activation and canonical pyroptosis, at least in part, through the suppression of the TLR4/NF-κB signaling pathway. These findings might provide new insights and a solid foundation for further exploration into the therapeutic potential of ruscogenin in the treatment of IBD.

Spatial-temporal evolution and spatial spillover of the green efficiency of urban construction land in the Yangtze River Economic Belt, China.

There are urgent ecological and environmental problems in the process of the utilization of urban construction land, promoting green utilization of construction land is conducive to urban sustainable development and high-quality economic development. Based on the panel data of 108 prefecture-level and above cities in the Yangtze River Economic Belt, China from 2003 to 2017, this paper uses the super-efficiency SBM model to measure the green efficiency of urban construction land (GEUCL), analyzes its spatial-temporal evolution characteristics, and constructs the spatial autoregressive model to study its spatial spillover effects from the perspective of urban hierarchy. It is found that, in terms of temporal variation, the average efficiency value shows a fluctuating upward trend during the study period, rising from 0.27 in 2003 to 0.39 in 2017, the cumulative growth rate is 44.44%, with an average annual growth rate of 3.14%. In terms of spatial distribution characteristics, during the study period, the number of medium-efficiency and high-efficiency cities increases significantly, while the number of low-efficiency cities decreases sharply; high-efficiency cities always present scattered distribution, while medium-efficiency cities change from scattered distribution to agglomeration distribution. In addition, GEUCL has significantly positive spatial spillover effects between neighboring cities of different grades and between neighboring cities of the same grade, among them, the increase of GEUCL in higher-grade cities has significantly positive spatial spillover effects on that in adjacent lower-grade cities; the increase of GEUCL in lower-grade cities has significantly positive spatial spillover effects on that in neighboring higher-grade cities; GEUCL has significantly positive spatial spillover effects between neighboring cities of the same grade.

Disc-condyle relationship alterations following stabilization splint therapy or arthrocentesis plus hyaluronic acid injection in patients with anterior disc displacement: a retrospective cohort study.

OBJECTIVE: The objective of this study was to quantitatively evaluate the efficacy of stabilization splint (SS) therapy or arthrocentesis plus hyaluronic acid (HA) injection in the treatment of anterior disc displacement (ADD) through magnetic resonance imaging (MRI). METHODS: 99 subjects were collected in this study. 46 subjects received SS treatment (SS group), 53 subjects received arthrocentesis plus HA injection (HA group). Joints with anterior disc displacement with reduction (ADDwR) and anterior disc displacement without reduction (ADDwoR) were compared separately. MRI before the beginning of the treatment and after a set of treatment were used for measurement. Disc-condyle relationship and positions of condyles and discs were determined by disc-condyle angles and X-Y coordinates. RESULTS: The disc-condyle angles decreased significantly in the SS group (P < .0001). Whereas no significant change was found in the HA group. Substantial anteroinferior condyle movement was detected in the SS group, slight anterior movement of condyles was discovered in the HA group. Anterior shift of discs position was observed in HA group and joints with ADDwoR in the SS group. CONCLUSIONS: SS was effective in improving the disc-condyle relationship in ADD subjects, while significant improvement of disc-condyle relationship cannot be achieved through arthrocentesis plus HA injection.