Molecular mechanism and functional significance of Wapl interaction with the Cohesin complex.

The ring-shaped Cohesin complex, consisting of core subunits Smc1, Smc3, Scc1, and SA2 (or its paralog SA1), topologically entraps two duplicated sister DNA molecules to establish sister chromatid cohesion in S-phase. It remains largely elusive how the Cohesin release factor Wapl binds the Cohesin complex, thereby inducing Cohesin disassociation from mitotic chromosomes to allow proper resolution and separation of sister chromatids. Here, we show that Wapl uses two structural modules containing the FGF motif and the YNARHWN motif, respectively, to simultaneously bind distinct pockets in the extensive composite interface between Scc1 and SA2. Strikingly, only when both docking modules are mutated, Wapl completely loses the ability to bind the Scc1-SA2 interface and release Cohesin, leading to erroneous chromosome segregation in mitosis. Surprisingly, Sororin, which contains a conserved FGF motif and functions as a master antagonist of Wapl in S-phase and G2-phase, does not bind the Scc1-SA2 interface. Moreover, Sgo1, the major protector of Cohesin at mitotic centromeres, can only compete with the FGF motif but not the YNARHWN motif of Wapl for binding Scc1-SA2 interface. Our data uncover the molecular mechanism by which Wapl binds Cohesin to ensure precise chromosome segregation.

A Single Image 3D Reconstruction Method Based on a Novel Monocular Vision System.

Three-dimensional (3D) reconstruction and measurement are popular techniques in precision manufacturing processes. In this manuscript, a single image 3D reconstruction method is proposed based on a novel monocular vision system, which includes a three-level charge coupled device (3-CCD) camera and a ring structured multi-color light emitting diode (LED) illumination. Firstly, a procedure for the calibration of the illumination's parameters, including LEDs' mounted angles, distribution density and incident angles, is proposed. Secondly, the incident light information, the color distribution information and gray level information are extracted from the acquired image, and the 3D reconstruction model is built based on the camera imaging model. Thirdly, the surface height information of the detected object within the field of view is computed based on the built model. The proposed method aims at solving the uncertainty and the slow convergence issues arising in 3D surface topography reconstruction using current shape-from-shading (SFS) methods. Three-dimensional reconstruction experimental tests are carried out on convex, concave, angular surfaces and on a mobile subscriber identification module (SIM) card slot, showing relative errors less than 3.6%, respectively. Advantages of the proposed method include a reduced time for 3D surface reconstruction compared to other methods, demonstrating good suitability of the proposed method in reconstructing surface 3D morphology.

Dislocation Defect Layer-Induced Magnetic Bi-states Phenomenon in Epitaxial La0.7Sr0.3MnO3(111) Thin Films.

La0.7Sr0.3MnO3 (LSMO) is one of the most fascinating strongly correlated oxides in which the spin polarization and magnetic property are sensitive to strain, especially in the (111)-oriented LSMO. In the paper, epitaxial LSMO(111) thin films with different thicknesses were prepared, and they showed continuous dislocation defect arrays with thickness greater than 45 nm. Then, the thick LSMO(111) films were divided into a double-layer structure with two slightly different oriented cells. The LSMO(111) films present a stronger lattice-spin coupling, thus the double-layer structure triggers an obvious magnetic heterogeneity phenomenon (magnetic bi-states) by the way of creating a double-mode ferromagnetic resonance (FMR) spectrum. Therefore, the nanostructures, especially the ordered structure defects, may trigger enriched physical phenomena and offer new forms of spin coupling and device functionality in strain-sensitive strongly correlated oxide systems.

Flexible CoFeB/Silk Films for Biocompatible RF/Microwave Applications.

Aiming to realize the integration of signal processing with the human body, wearable and implantable radio frequency (RF)/microwave devices are rapidly developed. However, the insufficiency in skin commonality and biocompatibility brings up challenges in making such devices. In this study, an ultra-flexible and biocompatible CoFeB/silk film is developed potentially for on-skin and implantable RF/microwave applications. Moreover, the CoFeB/silk films present controllable dissolvability in aqueous solutions and exhibit great potentials in applications for environmentally friendly disposable devices. A strain-tunable bandstop filter based on the CoFeB/silk film was fabricated, which exhibited a large frequency tunability of 3 GHz and ability in monitoring the finger-joints' motions. This concept and method of combining ferromagnetic materials with the biocompatible substrate offers a novel pathway for wearable/implantable applications.

An injectable supramolecular self-healing bio-hydrogel with high stretchability, extensibility and ductility, and a high swelling ratio.

Reversible networks are a key factor for designing self-healing hydrogels with high stretching properties. To achieve that, it is often necessary to modify or graft functional groups to the main chains for inducing the formation of reversible covalent-bond-based chemical cross-linking or hydrogen-bond-based physical cross-linking, thus leading to a complicated chemical process and high cost. Here, we proposed a dynamic sliding physical crosslinking mechanism of chains to design and synthesize hydrogels with both good self-healing ability and extensibility by introducing interstitial phases of small organic molecules into the hydrogel networks to enhance hydrogen bonds, which has been proved to be a quite facile and practical approach to achieve stretchable and self-healing properties. Our work might greatly promote our ability to understand the role of hydrogen bonds that are often overlooked in the design of materials. The as-synthesized hydrogels displayed extraordinary swelling properties with a swelling ratio of 2750% in PBS and of nearly 10 000% in stilled water, respectively, and they also showed excellent performance after many stress cycles under 95% compressive deformation. The use of 10% diethylene glycol could allow the elongation to be increased from 238% to 2705%. Our cell and animal experimental studies indicated that the as-synthesized supramolecular hydrogels have good biocompatibility and bioactivity and show potential for clinical application.