RESUMEN
Anti-inflammatory component (AIC) known as an important constituent of artificial musk exhibits bioactive effects on many pharmacological models. This study describes an immunochemical assay for the quality control of artificial musk in traditional Chinese medicine using an enzyme-linked immunosorbent assay. A polyclonal antibody against AIC was produced by rabbit. The method, at an effective measuring range of 3.13-200 ng/ml of AIC, 3 ng/ml limit of detection, and no cross-reaction with natural musk, successfully detected artificial musk in many traditional Chinese prescriptions containing artificial musk. The results demonstrated that a novel and reliable assay system for detection of artificial musk was generated.
Asunto(s)
Antiinflamatorios/farmacología , Ensayo de Inmunoadsorción Enzimática/métodos , Ácidos Grasos Monoinsaturados , Medicina Tradicional China , Animales , Antiinflamatorios/análisis , Antiinflamatorios/inmunología , Ácidos Grasos Monoinsaturados/análisis , Ácidos Grasos Monoinsaturados/química , Ácidos Grasos Monoinsaturados/farmacología , Masculino , Estructura Molecular , ConejosRESUMEN
In this letter, (R)-muscone and (S)-muscone were prepared by optical resolution of dl-muscone using N,N'-dibenzyl-l-tartaramide or N,N'-dibenzyl-d-tartaramide, according to the method reported by Kunihiko Takabe. But we separated the diastereomers using EtOH instead of MeOH in the recrystallization step to get the goal product with higher optical purity and yield. The regulatory effect of muscone and its enantiomer on the neural system showed that they could prolong mouse hypoxia tolerance and dose-dependently enhance mouse spinal cord stimulation induced by strychnine nitrate. (R)-muscone and (S)-muscone had a synergistic action on shortening sleep time induced by sodium pentobarbital in mice.
Asunto(s)
Cicloparafinas/química , Hipocampo/fisiología , Sistema Nervioso/efectos de los fármacos , Neuronas/fisiología , Animales , Cicloparafinas/farmacología , Relación Dosis-Respuesta a Droga , Hipoxia/metabolismo , Ratones , Estructura Molecular , Fenobarbital/farmacología , Estereoisomerismo , Estricnina/farmacologíaRESUMEN
OBJECTIVE: To identify the anti-inflammatory effects of artificial musk aqueous extract(AME)on lipopolysaccharide-stimulated cytokines secreted or released by RAW264.7 cells. METHODS: Cytokines including interleukin(IL)-6,IL-10,and tumor necrosis factor Α were determined using cytokine enzyme-linked immunosorbent assay kits. RESULT: Compared with model group,the levels of major cytokines such as tumor necrosis factor Α,IL-6,and IL-10 significantly decreased in different AME groups in a dose-dependent manner. CONCLUSION: In lipopolysaccharide-stimulated RAW264.7 macrophages,AME can remarkably inhibit the release of inflammatory cytokines and thus exerts its anti-inflammatory effects.
Asunto(s)
Antiinflamatorios/farmacología , Ácidos Grasos Monoinsaturados/farmacología , Mediadores de Inflamación/metabolismo , Animales , Línea Celular Tumoral , Citocinas/metabolismo , Ensayo de Inmunoadsorción Enzimática , Interleucina-6/metabolismo , Lipopolisacáridos , Macrófagos , Factor de Necrosis Tumoral alfa/metabolismoRESUMEN
OBJECTIVE: To investigate the effects of Musk glucoprotein on chemotaxis of Polymorphonuclear leukocytes(PMN). METHOD: The chemotaxis of PMN in abdominal cavity in rat induced by carboxymethyl cellulose(CMC) was used as an in vivo animal model and in in vitro it was evaluated by Boyden chamber. The concentration of cytosolic free Ca2+ was quantitated with the fluorescent Ca2+ indicator Fura-2. RESULT: The water extract of Musk at dose of 5, 20, 80 mg.kg-1 (s.c.) significantly inhibited the chemotaxis of PMN in rat; Musk-1 at concentration of 1-100 micrograms.mL-1 can significantly inhibit the chemotaxis of rabbit PMN in vitro; Musk-1 at concentration of 1-100 micrograms.mL-1 can significantly inhibit the increase of cytosolic Ca2+ concentration in PMN of rat. CONCLUSION: Part of mechanisms underlying antiinflammatory action of Musk is to inhibit the chemotaxis of PMN.