Feeding broilers with wheat germ, hops and grape seed extract mixture improves growth performance.

In the study, Wheat germ, Hops and Grape seed extracts were made into a mixture (BX). The BX was supplemented in AA + broilers diets to investigate the effects of BX on broiler growth performance, blood indicators, microbiota, and noxious gas emissions in faeces. Four hundred and eighty 1-day-old AA + male broilers with an average initial body weight (44.82 ± 0.26) were randomly divided into four dietary treatments of six replicates each, with 20 birds per replicate. The experimental groups consisted of a group fed a basal diet and groups fed basal diet supplemented with 0.05%, 0.1%, and 0.2% BX. The trail was 42 days. The results showed that supplementing the dietary with graded levels of BX linearly increased ADG and ADFI from days 22-42 and 1-42. When dietarys supplemented with 0.2% BX significantly increased ADG and ADFI on days 22-42 and 1-42 (p < 0.05). The addition of BX reduced H2S and NH3 emissions in the faeces; the levels of E. coli and Salmonella in the faeces were significantly reduced and the levels of Lactobacillus were increased (p < 0.05). In this trial, when the diet was supplemented with 0.2% BX, faecal levels of E. coli and Salmonella were consistently at their lowest levels and Lactobacillus were at their highest. At the same time, NH3 and H2S emissions from broiler faecal also had been at their lowest levels. Conclusion: Dietary supplementation with a 0.2% BX could improve the growth performance of broilers and also reduced faecal H2S and NH3 emissions, as well as faecal levels of E. coli and Salmonella, and increased levels of Lactobacillus. Thus, BX made by Wheat germ, Hops and Grape seed extract is expected to be an alternative to antibiotics. And based on the results of this trial, the recommended dose for use in on-farm production was 0.2%.

Sodium dichloroisocyanurate: improving broiler health by reducing harmful microbial levels in the waterline.

Sodium dichloroisocyanurate (NaDCC) is commonly used for treating drinking water, industrial water, and wastewater. This study aimed to investigate the potential effects of NaDCC-treated waterline drinking water on the growth of AA+ broilers by reducing microbial levels in the waterline. A total of 480 healthy 1-day-old AA+ broilers (46.77 ± 0.50 g) were selected for the experiment and randomly divided into four groups with six replicates of 20 birds each. The control group received regular drinking water, while the test groups received drinking water with NaDCC concentrations of 10, 30, and 50 mg/L. The test groups consumed the treated water on specific days throughout the 42-day experimental period. Results showed that NaDCC treatment significantly reduced the levels of E. coli, Salmonella, S. aureus and Moulds in the drinking water at the waterline (p < 0.05). Drinking water with NaDCC also led to reduced broiler fecal emissions of NH3 and H2S, as well as reduced counts of E. coli, Salmonella, S. aureus and Moulds (p < 0.05), particularly at 30 mg/L and 50 mg/L concentrations. Broilers consuming NaDCC at 50 mg/L exhibited a significant increase in ADG from days 1-42 (p < 0.05). The levels of E. coli, Salmonella, S. aureus and Moulds in the drinking water at the waterline were significantly and positively correlated with the bacterial count in the feces (p < 0.05, R > 0.6). Additionally, bacterial levels in drinking water and broiler feces were negatively correlated with broiler production performance indicators, including ADG, ADFI, F/G and AWC. In conclusion, NaDCC can indirectly enhance broiler performance by reducing the levels of harmful bacteria in the waterline without affecting normal drinking water. The addition of 30 mg/L or 50 mg/L of NaDCC to the waterline in poultry production can effectively control harmful microorganisms and improve poultry health. Based on the experiment's results, it is recommended to preferentially use 30 mg/L NaDCC in the waterline to reduce farming costs.

Growth performance and cecal microbiota of broiler chicks as affected by drinking water disinfection and/or herbal extract blend supplementation.

Environmental exposures during early life are important for animals' intestinal microbiota composition and their production performance. This experiment investigated the growth performance, hematology parameters, jejunal morphology, and cecal microbiota of broiler chicks as affected by exogenous factors from the aspects of drinking water quality and dietary manipulation. A total of 480-day-old broiler chicks (Arbor acre; 41.59 ± 0.88 g) were randomly assigned into 4 groups (CON, HWGM, CA, CAHWGM). Each group had 6 replicates with 20 birds per replicate. Broiler chicks in CON group were fed with basal diet and drank normal drinking water; in HWGM group were fed with basal diet supplemented with 1.5g/kg herbal extract blend (hops, grape seed, and wheat germ) and drank normal drinking water; in CA group were fed with basal diet and drank sodium dichlorocyanurate (50 mg/L) treated-drinking water; in CAHWGM group were fed with basal diet supplemented with 1.5 g/kg herbal extract blend and drank chlorinated drinking water. The experimental period was 42 d. We found that broiler chicks drank chlorinated drinking water led to an increase in body weight gain and feed efficiency during d 22 to 42 and 1 to 42, as well as a decrease in cecal Dysgonomonas and Providencia abundance. Dietary supplementation of herbal extract blend increased cecal Lactobacillus and Enterococcus abundance, whereas decreased Dysgonomonas abundance. Moreover, we observed that cecal Dysgonomonas abundance synergistically decreased by treating drinking water with sodium dichlorocyanurate and supplementing herbal extract blend to the diet. Therefore, results obtained in this study indicated that providing chlorinated drinking water is an effective strategy to improve the growth performance of broiler chicks by regulating intestinal microbiota. Additionally, dietary supplementation of herbal extract blend alone or combined with chlorinated drinking water is able to regulate cecal microbiota.

Regulating effects of chlorinated drinking water on cecal microbiota of broiler chicks.

In this study, 2 types of drinking water were provided to broiler chicks to evaluate the relationship between the bacterial load of drinking water and cecal microbiota. One type of drinking water was untreated, while the other type was daily treated with sodium dichlorocyanurate (50 mg/L). A total of 240 broiler chicks were divided into 2 groups based on their initial body weight. There were 6 replicates in each group, and each replicate cage contained 20 birds. Each cage was assigned to a different floor of the battery cage. On the final day, water samples were collected from each replicate cage at the opening of the drinking cup height, and one bird was selected from each replicate cage to obtain cecal content samples for measuring microbiota composition using the 16S rRNA technique. We found that drinking water treated with sodium dichlorocyanurate significantly reduced the richness and diversity of microbiota and diminished/disappeared most gram-negative bacteria. Broiler chicks that consumed chlorinated drinking water exhibited changes in the composition of cecal microbiota, with Alistipes serving as the marker species in the cecal content of broiler chicks that consumed untreated water, whereas AF12 served as the marker species in the cecal content of broiler chicks that consumed chlorinated drinking water. Functional prediction using the MetaCyc database and species composition analysis of metabolic pathways showed that changes in 7 metabolic pathways were related to the abundance of Providencia. Therefore, we concluded that chlorinated drinking water reduced the bacterial load in drinking water, thereby altering the cecal microbiota composition and regulating the metabolic activity of broiler chicks.

Effects of dietary supplementation probiotic complex on growth performance, blood parameters, fecal harmful gas, and fecal microbiota in AA+ male broilers.

In this study, Bacillus subtilis, Clostridium butyricum and Enterococcus faecalis were made into a probiotic complex (PC). The PC was supplemented in AA+ male broilers' diets to investigate the effects of PC on broiler growth performance, carcass traits, blood indicators, harmful gas emissions in feces and microbiota. Three hundred and sixty 1-day-old AA+ male broilers with an average initial body weight (data) were randomly divided into 3 dietary treatments of 6 replicates each, with 20 birds per replicate. The control group (T0) was fed a basal diet, while the test groups (T1 and T2) were supplemented with 0.025 and 0.05% PC in the basal diet, respectively. The trail was 42 days. The results showed that the supplementation of 0.05% PC significantly (p < 0.05) improved average daily gain (ADG) and average daily feed intake (ADFI) of broilers from 22 to 42 days and 1-42 days. Compared to the control group, the breast rate was significantly higher in T2, and the thymic index was significantly higher than that in T1 treatment (p < 0.05). The addition of PC had no significant effects on antibody potency in broiler serum (p > 0.05), but significantly increased albumin and total protein content in serum (p < 0.05). The addition of PC reduced H2S and NH3 emissions in the feces; the levels of Escherichia coli and Salmonella in the feces were significantly reduced and the levels of Lactobacillus were increased. And the most significant results were achieved when PC was added at 0.05%. Correlation analysis showed a significant positive correlation (p < 0.05) between the levels of E. coli and Salmonella and the emissions of H2S and NH3. Conclusion: Dietary supplementation with a 0.05% probiotic complex could improve the growth performance of broilers and also reduced fecal H2S and NH3 emissions, as well as fecal levels of E. coli and Salmonella, and increased levels of Lactobacillus. Thus, PC made by Bacillus subtilis, Clostridium butyricum and Enterococcus faecalis is expected to be an alternative to antibiotics. And based on the results of this trial, the recommended dose for use in on-farm production was 0.05%.

Effect of multi-strain probiotics on the performance of AA+ male broilers.

The aim of the experiment was to investigate the effects of a probiotic complex (PC) consisting of Bacillus subtilis, Clostridium butyricum and Enterococcus faecalis on productive performance, carcass traits, immune organ indices, fecal microbiota counts and noxious gas emissions in AA+ male broilers. Three hundred and sixty 1-day-old AA+ male broilers with similar body weight (44.77 ± 0.25) were randomly divided into 3 treatment groups of 6 replicates each, with 20 broilers in each replicate. The experimental groups consisted of a group fed a basal diet and groups fed basal diet supplemented with 0.1 and 0.2% PC. The results showed that the addition of PC had no significant effect (P > 0.05) on growth performance, and carcass traits of AA+ broilers during the experimental period (1-42 days of age). Dietary addition of PC significantly increased the thymus index of AA+ broilers (P < 0.05), reduced the number of E. coli and Salmonella in feces (P < 0.01) and reduced the concentrations of fecal NH3 and H2S emissions (P < 0.01). Furthermore, birds fed 0.2% PC diet had the highest number of fecal Lactobacillus counts. Results indicate that probiotic complex consisting of Bacillus subtilis, Clostridium butyricum and Enterococcus faecalis enhances immune organ development, reduces the number of E. coli and Salmonella in feces, increases the number of Lactobacillus and reduces NH3 and H2S emissions in feces. This trial provides a theoretical basis for the use of probiotic complexes in broiler production.

Feeding Broiler Chicks with Bacillus subtilis, Clostridium butyricum, and Enterococcus faecalis Mixture Improves Growth Performance and Regulates Cecal Microbiota.

A total of 300 day-old Arbor Acres Plus broiler chicks (mixed sex) was used to evaluate the effects of dietary supplementation of Bacillus subtilis, Clostridium butyricum, and Enterococcus faecalis mixture (PB) on growth performance, ileal morphology, and cecal microbiota. All birds were randomly assigned into 3 groups based on the initial body weight. There were 5 replicate cages per group and 20 birds per cage. The experimental period was 42 days. Dietary treatments were based on a basal diet and supplemented with 0, 0.05, or 0.10% PB. The results indicated that broiler chicks fed with the diet supplemented with graded levels of PB have quadratically improved their body weight gain and feed intake; the highest value was presented in 0.05% PB-containing group. In addition, villus to crypt ratio linearly increased with the concentration of PB increased in the diet. The alpha diversity linearly increased by PB supplementation, and the highest value was presented in 0.10% PB-containing group. In terms of growth performance, the suitable dose of PB used in the diet was 0.05%. However, ternary plot showed that the harmful bacteria, Escherichia-Shigella, was enriched in 0.05% PB-containing group. In brief, we considered that dietary supplementation of graded levels of PB improved growth performance and regulated cecal microbiota in broiler chicks.