Genetic mapping reveals a dominant awn-inhibiting gene related to differentiation of the variety anathera in the wild diploid wheat Aegilops tauschii.

Aegilops tauschii, a wild wheat relative, is the D-genome donor of common wheat. Subspecies and varieties of Ae. tauschii are traditionally classified based on differences in their inflorescence architecture. However, the genetic information for their diversification has been quite limited in the wild wheat relatives. The variety anathera has no awn on the lemma, but the genetic basis for this diagnostic character is unknown. Wide variations in awn length traits at the top and middle spikes were found in the Ae. tauschii core collection, and the awn length at the middle spike was significantly smaller in the eastward-dispersed sublineage than in those in other sublineages. To clarify loci controlling the awnless phenotype of var. anathera, we measured awn length of an intervariety F2 mapping population, and found that the F2 individuals could be divided into two groups mainly based on the awn length at the middle of spike, namely short and long awn groups, significantly fitting a 3:1 segregation ratio, which indicated that a single locus controls the awnless phenotype. The awnless locus, Anathera (Antr), was assigned to the distal region of the short arm of chromosome 5D. Quantitative trait locus analysis using the awn length data of each F2 individual showed that only one major locus was at the same chromosomal position as Antr. These results suggest that a single dominant allele determines the awnless diagnostic character in the variety anathera. The Antr dominant allele is a novel gene inhibiting awn elongation in wheat and its relatives.

Stomatal Traits and Barley (Hordeum vulgare L.) Forage Yield in Drought Conditions of Northeastern Mexico.

Infrared technology is a practical, fast, non-destructive method that helps in forecasting plant development and can be used to select physiological traits, instead of other methodologies that require more time and breeding efforts. According to the statistical analyses and the relationship between the direct and indirect effects of the variables, this technology could serve as the basis for implementing a genotype selection methodology. Awnless barley was assessed in a randomized block design with three replicates in two crop seasons at Mexico's northeastern region. Two samplings were carried out during crop development: at 75 and 90 days after sowing. The infrared and stomatal sensors were used to identify the direct and indirect effects of stomata's traits on dry forage yield. The data were analyzed in a subdivided plot design, using mean comparison tests, correlation coefficients and path analyses, finding significant differences (p < 0.05) among localities and among samplings. Dry forage yield was significant and positively correlated with plant height (r = 0.578) and canopy temperature (r = 0.724), and negatively correlated with the leaf upper side stomatal width (r = -0.409) and the leaf lower side stomatal width (r = -0.641), chlorophyll content and vegetation index. Temperature, chlorophyll, density and leaf lower side stomatal index had the strongest direct effects on yield. Therefore, the infrared technology appears as a way to select high yielding awnless forage barley, to obtain the correlation, the positive direct effect of temperature and the negative effect of chlorophyll. Due to their direct effects, low density and low leaf underside stomatal indexes can also help in the indirect selection of higher yielding forage barley genotypes.

Nanopore sequencing-based genome assembly and evolutionary genomics of circum-basmati rice.

BACKGROUND: The circum-basmati group of cultivated Asian rice (Oryza sativa) contains many iconic varieties and is widespread in the Indian subcontinent. Despite its economic and cultural importance, a high-quality reference genome is currently lacking, and the group's evolutionary history is not fully resolved. To address these gaps, we use long-read nanopore sequencing and assemble the genomes of two circum-basmati rice varieties. RESULTS: We generate two high-quality, chromosome-level reference genomes that represent the 12 chromosomes of Oryza. The assemblies show a contig N50 of 6.32 Mb and 10.53 Mb for Basmati 334 and Dom Sufid, respectively. Using our highly contiguous assemblies, we characterize structural variations segregating across circum-basmati genomes. We discover repeat expansions not observed in japonica-the rice group most closely related to circum-basmati-as well as the presence and absence variants of over 20 Mb, one of which is a circum-basmati-specific deletion of a gene regulating awn length. We further detect strong evidence of admixture between the circum-basmati and circum-aus groups. This gene flow has its greatest effect on chromosome 10, causing both structural variation and single-nucleotide polymorphism to deviate from genome-wide history. Lastly, population genomic analysis of 78 circum-basmati varieties shows three major geographically structured genetic groups: Bhutan/Nepal, India/Bangladesh/Myanmar, and Iran/Pakistan. CONCLUSION: The availability of high-quality reference genomes allows functional and evolutionary genomic analyses providing genome-wide evidence for gene flow between circum-aus and circum-basmati, describes the nature of circum-basmati structural variation, and reveals the presence/absence variation in this important and iconic rice variety group.